TOP2A (H. sapiens )
In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  TOP2A
Gene Name:  topoisomerase (DNA) II alpha 170kDa
Original Clone ID:
HR4666B-446-679-15.4
Keyword:
targeted domain
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pET15_NESG
Format: 
CLOSED
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI:Biology
Northeast Structural Genomics Consortium
HsCD00585338
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AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 708nts
Open reading frame: 51 to 758

CTTTAAGAAGGAGATATACCATGGGCCATCACCATCACCATCACAGCCATATGGATGCAG
GGGGCCGAAACTCCACTGAGTGTACGCTTATCCTGACTGAGGGAGATTCAGCCAAAACTT
TGGCTGTTTCAGGCCTTGGTGTGGTTGGGAGAGACAAATATGGGGTTTTCCCTCTTAGAG
GAAAAATACTCAATGTTCGAGAAGCTTCTCATAAGCAGATCATGGAAAATGCTGAGATTA
ACAATATCATCAAGATTGTGGGTCTTCAGTACAAGAAAAACTATGAAGATGAAGATTCAT
TGAAGACGCTTCGTTATGGGAAGATAATGATTATGACAGATCAGGACCAAGATGGTTCCC
ACATCAAAGGCTTGCTGATTAATTTTATCCATCACAACTGGCCCTCTCTTCTGCGACATC
GTTTTCTGGAGGAATTTATCACTCCCATTGTAAAGGTATCTAAAAACAAGCAAGAAATGG
CATTTTACAGCCTTCCTGAATTTGAAGAGTGGAAGAGTTCTACTCCAAATCATAAAAAAT
GGAAAGTCAAATATTACAAAGGTTTGGGCACCAGCACATCAAAGGAAGCTAAAGAATACT
TTGCAGATATGAAAAGACATCGTATCCAGTTCAAATATTCTGGTCCTGAAGATGATGCTG
CTATCAGCCTGGCCTTTAGCAAAAAACAGATAGATGATCGAAAGGAATGGTTAACTAATT
TCATGGAGGATAGAAGACAACGAAAGTTACTTGGGTAGCTCGAGATCCGGCTGCTAACAA
AGCCCGAAA

Protein sequence predicted based on nucleotide sequence

MDAGGRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMEN
AEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSL
LRHRFLEEFITPIVKVSKNKQEMAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEA
KEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQIDDRKEWLTNFMEDRRQRKLLG*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in HR4666B
Recommended expression in:
Not Applicable