DNASU Plasmid | PIP4K2A (H. sapiens )<br>In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: PIP4K2A

Gene Name: phosphatidylinositol-5-phosphate 4-kinase, type II, alpha

Original Clone ID:

HR3110-14.5

Keyword:

full length protein truncation

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pET15_NESG

Format:

CLOSED

Source:

Northeast Structural Genomics Consortium

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

PSI:Biology
Northeast Structural Genomics Consortium

HsCD00585414

Price:

AVAILABLE

No restriction
Special MTA: PSI/NESG

Insert Sequence
Protein Sequence

Insert sequence: 1221nts

Open reading frame: 15 to 1235

Reference Sequence Alignment

ACCATCACAGCCATATGGCGACCCCCGGCAACCTAGGGTCCTCTGTCCTGGCGAGCAAGA
CCAAGACCAAGAAGAAGCACTTCGTAGCGCAGAAAGTGAAGCTGTTTCGGGCCAGCGACC
CGCTGCTCAGCGTCCTCATGTGGGGGGTAAACCACTCGATCAATGAACTGAGCCATGTTC
AAATCCCTGTTATGTTGATGCCAGATGACTTCAAAGCCTATTCAAAAATAAAGGTGGACA
ATCACCTTTTTAACAAAGAAAACATGCCGAGCCATTTCAAGTTTAAGGAATACTGCCCGA
TGGTCTTCCGTAACCTGCGGGAGAGGTTTGGAATTGATGATCAAGATTTCCAGAATTCCC
TGACCAGGAGCGCACCCCTCCCCAACGACTCCCAGGCCCGCAGTGGAGCTCGTTTTCACA
CTTCCTACGACAAAAGATACATCATCAAGACTATTACCAGTGAAGACGTGGCCGAAATGC
ACAACATCCTGAAGAAATACCACCAGTACATAGTGGAATGTCATGGGATCACCCTTCTTC
CCCAGTTCTTGGGCATGTACCGGCTTAATGTTGATGGAGTTGAAATATACGTGATAGTTA
CAAGAAATGTATTCAGCCACCGTTTGTCTGTGTATAGGAAATACGACTTAAAGGGCTCTA
CAGTGGCTAGAGAAGCTAGTGACAAAGAAAAGGCCAAAGAACTGCCAACTCTGAAAGATA
ATGATTTCATTAATGAGGGCCAAAAGATTTATATTGATGACAACAGCAAGAAGGTCTTCC
TGGAAAAACTAAAAAAGGATGTTGAGTTTCTGGCCCAGCTGAAGCTCATGGACTACAGTC
TGCTGGTGGGAATTCATGATGTGGAGAGAGCCGAACAGGAGGAAGTGGAGTGTGAGGAGA
ACGATGGGGAGGAGGAGGGCGAGAGCGATGGCACCCACCCGGTGGGAACCCCCCCAGATA
GCCCCGGGAATACACTGAACAGCCCACCACCCCTGGCTCCCGGGGAGTTCGATCCGAACA
TCGACGTCTATGGAATTAAGTGCCATGAAAACTCGCCTAGGAAGGAGGTGTACTTCATGG
CAATTATTGACATCCTTACTCATTATGATGCAAAAAAGAAAGCTGCCCATGCTGCAAAAA
CTGTTAAACATGGCGCTGGCGCGGAGATCTCCACCGTGAACCCAGAACAGTATTCAAAGC
GCTTTTTGGACTTTATTGGCCACATCTTGACGTAG

Protein sequence predicted based on nucleotide sequence

MATPGNLGSSVLASKTKTKKKHFVAQKVKLFRASDPLLSVLMWGVNHSINELSHVQIPVM
LMPDDFKAYSKIKVDNHLFNKENMPSHFKFKEYCPMVFRNLRERFGIDDQDFQNSLTRSA
PLPNDSQARSGARFHTSYDKRYIIKTITSEDVAEMHNILKKYHQYIVECHGITLLPQFLG
MYRLNVDGVEIYVIVTRNVFSHRLSVYRKYDLKGSTVAREASDKEKAKELPTLKDNDFIN
EGQKIYIDDNSKKVFLEKLKKDVEFLAQLKLMDYSLLVGIHDVERAEQEEVECEENDGEE
EGESDGTHPVGTPPDSPGNTLNSPPPLAPGEFDPNIDVYGIKCHENSPRKEVYFMAIIDI
LTHYDAKKKAAHAAKTVKHGAGAEISTVNPEQYSKRFLDFIGHILT*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

t576c,Y192Y;a752g,N251S;

t970c,S324P

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinExpressed: Protein_Confirmed

ProteinPurified: Protein_Purified

SolubleProtein: Protein_Soluble

Find experimental protocols in HR3110

Recommended expression in:

Not Applicable

Vector Name:

pET15_NESG

Synonyms:

pET15, pET15-modified, pET15Cc, pET15_NESG

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.

Comments:

Modified Novagen pET vector-common MCS

Size (bp):

5723

Parent Vector:

None

Properties:

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker

Author Name:

Gaetano T. Montleione
Jessica Bearden
Northeast Structural Genomics Consortium

Publications:

PMID: 15808222

Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:

Vector Sequence:

Sequence:

TTCTCATGTTTGACAGCTTATCATCGATAAGCTAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCGAGAAGCTTTGCGTCGACAGAGCTCGCAGGATCCGCAGAATTCGCCATATGGCTGTGATGGTGATGGTGATGGCCCATGGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAA

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI)

327

452

bacterial origin

ori

ColE1 origin of replication

3881

4563

gene fragment

ROP

repressor of primer ROP sequence

3281

3457

primer

T7 forward

T7 forward sequencing primer

481

496

primer

T7 reverse

T7 reverse sequencing primer

257

275

promoter

T7 promoter

478

497

repressor binding site

lacO

lacO regulatory sequence

456

478

selectable marker

AmpR

ampicillin resistance gene

4661

5230

tag

His

N-terminal 6x His tag

387

404

trxn termination sequence

T7 term

T7 termination sequence

257

275

Gene

Gene Symbol :

PIP4K2A

Symbol Nomenclature :

PIP4K2A

SYNONYM :

PI5P4KA; PIP5K2A; PIP5KII-alpha; PIP5KIIA; PIPK

Designation :

Full Nomenclature :

phosphatidylinositol-5-phosphate 4-kinase, type II, alpha

GENEID :

5305

Locus Tag :

RP11-301N24.1

GI :

6857820

GenBank Accession :

NM_005028

HGNC :

8997

MIM :

603140

Vega :

OTTHUMG00000017810

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00585414      ACCATCACAGCCATATGGCGACCCCCGGCAACCTAGGGTCCTCTGTCCTGGCGAGCAAGA
NM_005028.4       --------------ATGGCGACCCCCGGCAACCTAGGGTCCTCTGTCCTGGCGAGCAAGA
                                **********************************************

HsCD00585414      CCAAGACCAAGAAGAAGCACTTCGTAGCGCAGAAAGTGAAGCTGTTTCGGGCCAGCGACC
NM_005028.4       CCAAGACCAAGAAGAAGCACTTCGTAGCGCAGAAAGTGAAGCTGTTTCGGGCCAGCGACC
                  ************************************************************

HsCD00585414      CGCTGCTCAGCGTCCTCATGTGGGGGGTAAACCACTCGATCAATGAACTGAGCCATGTTC
NM_005028.4       CGCTGCTCAGCGTCCTCATGTGGGGGGTAAACCACTCGATCAATGAACTGAGCCATGTTC
                  ************************************************************

HsCD00585414      AAATCCCTGTTATGTTGATGCCAGATGACTTCAAAGCCTATTCAAAAATAAAGGTGGACA
NM_005028.4       AAATCCCTGTTATGTTGATGCCAGATGACTTCAAAGCCTATTCAAAAATAAAGGTGGACA
                  ************************************************************

HsCD00585414      ATCACCTTTTTAACAAAGAAAACATGCCGAGCCATTTCAAGTTTAAGGAATACTGCCCGA
NM_005028.4       ATCACCTTTTTAACAAAGAAAACATGCCGAGCCATTTCAAGTTTAAGGAATACTGCCCGA
                  ************************************************************

HsCD00585414      TGGTCTTCCGTAACCTGCGGGAGAGGTTTGGAATTGATGATCAAGATTTCCAGAATTCCC
NM_005028.4       TGGTCTTCCGTAACCTGCGGGAGAGGTTTGGAATTGATGATCAAGATTTCCAGAATTCCC
                  ************************************************************

HsCD00585414      TGACCAGGAGCGCACCCCTCCCCAACGACTCCCAGGCCCGCAGTGGAGCTCGTTTTCACA
NM_005028.4       TGACCAGGAGCGCACCCCTCCCCAACGACTCCCAGGCCCGCAGTGGAGCTCGTTTTCACA
                  ************************************************************

HsCD00585414      CTTCCTACGACAAAAGATACATCATCAAGACTATTACCAGTGAAGACGTGGCCGAAATGC
NM_005028.4       CTTCCTACGACAAAAGATACATCATCAAGACTATTACCAGTGAAGACGTGGCCGAAATGC
                  ************************************************************

HsCD00585414      ACAACATCCTGAAGAAATACCACCAGTACATAGTGGAATGTCATGGGATCACCCTTCTTC
NM_005028.4       ACAACATCCTGAAGAAATACCACCAGTACATAGTGGAATGTCATGGGATCACCCTTCTTC
                  ************************************************************

HsCD00585414      CCCAGTTCTTGGGCATGTACCGGCTTAATGTTGATGGAGTTGAAATATACGTGATAGTTA
NM_005028.4       CCCAGTTCTTGGGCATGTACCGGCTTAATGTTGATGGAGTTGAAATATATGTGATAGTTA
                  ************************************************* **********

HsCD00585414      CAAGAAATGTATTCAGCCACCGTTTGTCTGTGTATAGGAAATACGACTTAAAGGGCTCTA
NM_005028.4       CAAGAAATGTATTCAGCCACCGTTTGTCTGTGTATAGGAAATACGACTTAAAGGGCTCTA
                  ************************************************************

HsCD00585414      CAGTGGCTAGAGAAGCTAGTGACAAAGAAAAGGCCAAAGAACTGCCAACTCTGAAAGATA
NM_005028.4       CAGTGGCTAGAGAAGCTAGTGACAAAGAAAAGGCCAAAGAACTGCCAACTCTGAAAGATA
                  ************************************************************

HsCD00585414      ATGATTTCATTAATGAGGGCCAAAAGATTTATATTGATGACAACAGCAAGAAGGTCTTCC
NM_005028.4       ATGATTTCATTAATGAGGGCCAAAAGATTTATATTGATGACAACAACAAGAAGGTCTTCC
                  *********************************************.**************

HsCD00585414      TGGAAAAACTAAAAAAGGATGTTGAGTTTCTGGCCCAGCTGAAGCTCATGGACTACAGTC
NM_005028.4       TGGAAAAACTAAAAAAGGATGTTGAGTTTCTGGCCCAGCTGAAGCTCATGGACTACAGTC
                  ************************************************************

HsCD00585414      TGCTGGTGGGAATTCATGATGTGGAGAGAGCCGAACAGGAGGAAGTGGAGTGTGAGGAGA
NM_005028.4       TGCTGGTGGGAATTCATGATGTGGAGAGAGCCGAACAGGAGGAAGTGGAGTGTGAGGAGA
                  ************************************************************

HsCD00585414      ACGATGGGGAGGAGGAGGGCGAGAGCGATGGCACCCACCCGGTGGGAACCCCCCCAGATA
NM_005028.4       ACGATGGGGAGGAGGAGGGCGAGAGCGATGGCACCCACCCGGTGGGAACCCCCCCAGATA
                  ************************************************************

HsCD00585414      GCCCCGGGAATACACTGAACAGCCCACCACCCCTGGCTCCCGGGGAGTTCGATCCGAACA
NM_005028.4       GCCCCGGGAATACACTGAACAGCTCACCACCCCTGGCTCCCGGGGAGTTCGATCCGAACA
                  *********************** ************************************

HsCD00585414      TCGACGTCTATGGAATTAAGTGCCATGAAAACTCGCCTAGGAAGGAGGTGTACTTCATGG
NM_005028.4       TCGACGTCTATGGAATTAAGTGCCATGAAAACTCGCCTAGGAAGGAGGTGTACTTCATGG
                  ************************************************************

HsCD00585414      CAATTATTGACATCCTTACTCATTATGATGCAAAAAAGAAAGCTGCCCATGCTGCAAAAA
NM_005028.4       CAATTATTGACATCCTTACTCATTATGATGCAAAAAAGAAAGCTGCCCATGCTGCAAAAA
                  ************************************************************

HsCD00585414      CTGTTAAACATGGCGCTGGCGCGGAGATCTCCACCGTGAACCCAGAACAGTATTCAAAGC
NM_005028.4       CTGTTAAACATGGCGCTGGCGCGGAGATCTCCACCGTGAACCCAGAACAGTATTCAAAGC
                  ************************************************************

HsCD00585414      GCTTTTTGGACTTTATTGGCCACATCTTGACGTAG
NM_005028.4       GCTTTTTGGACTTTATTGGCCACATCTTGACGTAA
                  **********************************.

Pathways

Reactome :

Metabolism

Reactome :

Metabolism of lipids and lipoproteins

Reactome :

Phospholipid metabolism

Reactome :

PI Metabolism

Reactome :

Synthesis of PIPs at the plasma membrane

WikiPathway :

G13 Signaling Pathway

WikiPathway :

Regulation of Actin Cytoskeleton

Protein

SMART domain :

PIPKc : Phosphatidylinositol phosphate kinases

Structural Annotation Wiki :

HR3110

UniProt :

P48426

HPRD :

11931

Clone

Cloning Information :

628603

Original Clone ID :

HR3110-14.5

Experimental

TargetTrack - Experimental data :

HR3110

Organism

Species Specific ID:

5305

Genome

Chromosome :

Map Location :

10p12.2

Ensembl :

ENSG00000150867

Reagents

Labome :

PIP4K2A-antibody

PIP4K2A (H. sapiens )In pET15_NESG (His-tagged bacterial expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Pathways

Protein

Clone

Experimental

Organism

Genome

Reagents

PIP4K2A (H. sapiens )
In pET15_NESG (His-tagged bacterial expression vector)