sahH (M. tuberculosis H37Rv ) in pMCSG73 (Bacterial Expression)

Explanation of Terms

Gene:
Gene Symbol:  sahH
Gene Name:  Probable adenosylhomocysteinase SahH (S-adenosyl-L-homocysteine hydrolase) (adohcyase)
Original Clone ID:
APC107490.103
Keyword:
None
Species:
Mycobacterium tuberculosis H37Rv
Type:
cDNA
Vector Name:
pMCSG73
Format: 
FUSION
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Argonne National Laboratory
PSI:Biology
Midwest Center for Structural Genomics
MtCD00585528
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1485nts
Open reading frame: 12 to 1496

AATCCAATGCCATGACCGGAAATTTGGTGACCAAAAATTCGCTGACCCCTGACGTTCGTA
ACGGCATCGACTTTAAGATCGCCGACCTGTCACTAGCGGATTTCGGCCGCAAAGAACTCC
GGATCGCCGAGCACGAGATGCCCGGCCTGATGTCGCTGCGGCGCGAGTATGCCGAGGTGC
AACCCCTGAAGGGGGCCCGGATCTCGGGTTCGCTGCACATGACGGTGCAGACCGCGGTGT
TGATCGAAACCCTCACCGCGCTGGGCGCCGAAGTCCGCTGGGCCTCGTGCAACATCTTCT
CCACCCAGGATCACGCCGCCGCCGCCGTCGTGGTCGGCCCGCACGGCACCCCCGACGAGC
CCAAGGGTGTCCCGGTGTTCGCGTGGAAGGGCGAGACGCTCGAAGAGTACTGGTGGGCCG
CCGAGCAGATGCTCACCTGGCCGGACCCCGACAAGCCGGCCAACATGATCCTCGATGACG
GCGGTGACGCCACCATGTTGGTGCTGCGCGGCATGCAGTATGAGAAGGCCGGCGTGGTGC
CGCCCGCCGAGGAGGACGACCCCGCCGAGTGGAAGGTCTTCCTGAACCTGCTACGGACCC
GCTTCGAGACCGACAAGGACAAGTGGACCAAGATAGCCGAGTCGGTCAAGGGCGTCACCG
AGGAGACCACCACCGGCGTGCTGCGGCTCTACCAATTCGCCGCGGCCGGGGATCTGGCCT
TCCCGGCGATCAACGTCAACGACTCGGTGACCAAGTCCAAATTCGACAACAAGTACGGCA
CTCGGCACTCCCTGATCGACGGCATCAACCGCGGCACCGACGCGCTGATCGGCGGTAAGA
AGGTCCTCATCTGCGGCTACGGCGACGTCGGTAAGGGCTGTGCGGAGGCGATGAAGGGCC
AGGGAGCGCGGGTCTCCGTCACCGAGATCGACCCGATCAACGCGCTGCAGGCCATGATGG
AGGGCTTCGACGTGGTCACCGTCGAGGAGGCCATCGGGGACGCCGACATCGTCGTAACCG
CGACCGGCAACAAAGACATCATCATGCTCGAGCACATTAAGGCGATGAAGGACCACGCGA
TCCTGGGAAATATCGGCCACTTCGACAACGAGATCGACATGGCCGGGCTGGAGCGCTCCG
GGGCGACACGGGTCAACGTCAAGCCTCAGGTCGACCTGTGGACCTTTGGCGACACGGGCC
GCTCGATCATCGTGCTGTCCGAGGGGCGGCTGCTGAACCTGGGCAATGCCACCGGGCACC
CCTCGTTCGTGATGAGCAACAGCTTCGCTAACCAGACGATCGCCCAGATCGAGCTGTGGA
CCAAGAACGACGAGTACGACAACGAGGTGTACCGGCTGCCCAAGCACCTCGACGAGAAGG
TGGCTCGAATCCATGTCGAGGCCCTTGGCGGTCACCTGACCAAGCTGACCAAGGAGCAGG
CCGAATACCTCGGCGTCGACGTCGAGGGTCCCTACAAGCCGGACCACTACCGCTAC

Protein sequence predicted based on nucleotide sequence

MTGNLVTKNSLTPDVRNGIDFKIADLSLADFGRKELRIAEHEMPGLMSLRREYAEVQPLK
GARISGSLHMTVQTAVLIETLTALGAEVRWASCNIFSTQDHAAAAVVVGPHGTPDEPKGV
PVFAWKGETLEEYWWAAEQMLTWPDPDKPANMILDDGGDATMLVLRGMQYEKAGVVPPAE
EDDPAEWKVFLNLLRTRFETDKDKWTKIAESVKGVTEETTTGVLRLYQFAAAGDLAFPAI
NVNDSVTKSKFDNKYGTRHSLIDGINRGTDALIGGKKVLICGYGDVGKGCAEAMKGQGAR
VSVTEIDPINALQAMMEGFDVVTVEEAIGDADIVVTATGNKDIIMLEHIKAMKDHAILGN
IGHFDNEIDMAGLERSGATRVNVKPQVDLWTFGDTGRSIIVLSEGRLLNLGNATGHPSFV
MSNSFANQTIAQIELWTKNDEYDNEVYRLPKHLDEKVARIHVEALGGHLTKLTKEQAEYL
GVDVEGPYKPDHYRY

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in MCSG-APC107490
Recommended expression in:
Not Applicable