MCM3 (H. sapiens )
In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  MCM3
Gene Name:  minichromosome maintenance complex component 3
Original Clone ID:
HR5454-228-808-14.14
Keyword:
targeted domain
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pET15_NESG
Format: 
CLOSED
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI:Biology
Northeast Structural Genomics Consortium
HsCD00585826
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AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 1749nts
Open reading frame: 14 to 1762

CCATCACAGCCATATGAAAGCGAAGCCTGGTGACCGGGTTCAGGTGGTGGGGACCTACCG
TTGCCTTCCTGGAAAGAAGGGAGGCTACACCTCTGGGACCTTCAGGACTGTCCTGATTGC
CTGTAATGTTAAGCAGATGAGCAAGGATGCTCAGCCCTCTTTCTCTGCTGAGGATATAGC
CAAGATCAAGAAGTTCAGTAAAACCCGATCCAAGGATATCTTTGACCAGCTGGCCAAGTC
ATTGGCCCCAAGTATCCATGGGCATGACTATGTCAAGAAAGCAATCCTCTGCTTGCTCTT
GGGAGGGGTGGAACGAGACCTAGAAAATGGCAGCCACATCCGTGGGGACATCAATATTCT
TCTAATAGGAGACCCATCCGTTGCCAAGTCTCAGCTTCTGCGGTATGTGCTTTGCACTGC
ACCCCGAGCTATCCCCACCACTGGCCGGGGCTCCTCTGGAGTGGGTCTGACGGCTGCTGT
CACCACAGACCAGGAAACAGGAGAGCGCCGTCTGGAAGCAGGGGCCATGGTCCTGGCTGA
CCGAGGCGTGGTTTGCATTGATGAATTTGACAAAATGTCTGACATGGATCGCACAGCCAT
CCATGAAGTGATGGAGCAGGGTCGAGTGACCATTGCCAAGGCTGGCATCCATGCTCGGCT
GAATGCCCGCTGCAGTGTTTTGGCAGCTGCCAACCCTGTCTACGGCAGGTATGACCAGTA
TAAGACTCCAATGGAGAACATTGGGCTACAGGACTCACTGCTGTCACGATTTGACTTGCT
CTTCATCATGCTGGATCAGATGGATCCTGAGCAGGATCGGGAGATCTCAGACCATGTCCT
TCGGATGCACCGTTACAGAACACCTGGGGAGCAGGATGGCGATGCTATGCCCTTGGGTAG
TGCTGTGGATATCCTGGCCACAGATGATCCCAACTTTAGCCAGGAAGATCAGCAGGACAC
CCAGATTTATGAGAAGCATGACAACCTTCTACATGGGACCAAGAAGAAAAAGGAGAAGAT
GGTGAGCGCAGCATTCATGAAGAAGTACATCTATGTGGCCAAAATCATCAAGCCTGTCCT
GACACAGGAGTCGGCCACCTACATTGCAGAAGAGTATTCACGCCTGCGCAGCCAGGATAG
CATGAGCTCAGACACCGCCAGGACATCTCCAGTTACAGCCCGAACACTGGAAACTCTGAT
TCGACTGGCCACAGCCCATGCGAAGGCCCGCATGAGCAAGACTGTGGACCTGCAGGATGC
AGAGGAAGCTGTGGAGTTGGTCCAGTATGCTTACTTTAAGAAGGTTCTGGAGAAGGAGAA
GAAACGTAAGAAGCGAAGTGAGGATGAATCAGAGACAGAAGATGAAGAGGAGAAAAGCCA
AGAGGACCAGGAGCAGAAGAGGAAGAGAAGGAAGACTCGCCAGCCAGATGCCAAAGATGG
GGATTCATACGACCCCTATGACTTCAGTGACACAGAGGAGGAAATGCCTCAAGTACACGC
TCCAAAGACGGCAGACTCACAGGAGACCAAGGAATCCCAGAAAGTGGAGTTGAGTGAATC
CAGGTTGAAGGCATTCAAGGTGGCCCTCTTGGATGTGTTCCGGGAAGCTCATGCGCAGTC
AATCGGCATGAATCGCCTCACAGAATCCATCAACCGGGACAGCAAAGAGCCCTTCTCTTC
AGTTGAGATCCAGGTTGCTCTGAGCAAGATGCAGGATGACAATCAGGTCATGGTGTCTGA
GGGCATCATCTTCCTCATCTAG

Protein sequence predicted based on nucleotide sequence

MKAKPGDRVQVVGTYRCLPGKKGGYTSGTFRTVLIACNVKQMSKDAQPSFSAEDIAKIKK
FSKTRSKDIFDQLAKSLAPSIHGHDYVKKAILCLLLGGVERDLENGSHIRGDINILLIGD
PSVAKSQLLRYVLCTAPRAIPTTGRGSSGVGLTAAVTTDQETGERRLEAGAMVLADRGVV
CIDEFDKMSDMDRTAIHEVMEQGRVTIAKAGIHARLNARCSVLAAANPVYGRYDQYKTPM
ENIGLQDSLLSRFDLLFIMLDQMDPEQDREISDHVLRMHRYRTPGEQDGDAMPLGSAVDI
LATDDPNFSQEDQQDTQIYEKHDNLLHGTKKKKEKMVSAAFMKKYIYVAKIIKPVLTQES
ATYIAEEYSRLRSQDSMSSDTARTSPVTARTLETLIRLATAHAKARMSKTVDLQDAEEAV
ELVQYAYFKKVLEKEKKRKKRSEDESETEDEEEKSQEDQEQKRKRRKTRQPDAKDGDSYD
PYDFSDTEEEMPQVHAPKTADSQETKESQKVELSESRLKAFKVALLDVFREAHAQSIGMN
RLTESINRDSKEPFSSVEIQVALSKMQDDNQVMVSEGIIFLI*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
a39g,G13G;g847a,A283T;

t1014c,S338S;c1039t,H347Y;

a1486g,T496A;g1651a,E551K;

c1682t,A561V
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in HR5454
Recommended expression in:
Not Applicable