PAS_chr1-4_0126 (P. pastoris )
In pSGP46 (ZZ and His-tagged yeast expression vector)

Explanation of Terms

Gene:
Gene Symbol:  PAS_chr1-4_0126
Gene Name:  Palmitoyltransferase for Vac8p, required for vacuolar membrane fusion
Original Clone ID:
PFA3-4_pSGP46
Keyword:
None
Species:
Pichia pastoris
Type:
cDNA
Vector Name:
pSGP46
Format: 
FUSION
Source:
Membrane Protein Structural Biology Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Rochester
PSI:Biology
Membrane Protein Structural Biology Consortium
PpCD00585984
Price: 
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1071nts
Open reading frame: 40 to 1110

ACGCAACTTCGAACACCTTTCTTACACAAATATACCAAAATGTATTATATCAAAAGATGC
TGCTGCCTTCTGGCGTCCACATTCCCGAGGGCCTTTTGCAGCGGATTGTTCCTTTGGGCC
ACCTACGTACTGTGCATTGAGATTGCTTTCAAGTTATACGGCGGAATAATAGGGCTGGTT
CTTTGCACTGTTGGTGTAATTCTACTAGTCATCTCCTTGCAGGCGTACTGTTTGGTAGTT
GCCTTAGGTCCTGGATCTCCGGCTGACTTTACCGAGCTTCATATCTTAAACTACGTTCCT
GGGATGCAGATCAAACCACCCCACGAACTGCTAAACAGAACAGTTACCACCAGTGGAAGT
GGCAATTTCAGGATATGCAATAAGTGTGAAGTGTTCAAACCAGATAGAAGTCACCACTGC
TCTTCTTGTGGTATATGTATACTCAAAATGGACCATCATTGTCCTTGGTTTGCATGTTGC
ATTGGTTTCAAGAATCACAAATTTTTTGTTCAATTTCTGATCTATACACAGATATATTCC
TTATTAGCATTGCTGGTCAGTGGGAAGGTTCTTTACGATTTTTTTGAGACGAAGAGGTAT
AAGGATCATTATTTATCTCTGAACTATGTGTTCTTAACGGTTGTTAGTTTTGTGACTTTT
CTTTCGTTAACCTTTTTCATAGGGTTCACCCTGTACCAACTATTTCGTAACAAAACCACG
ATCGAATCCTACGAAAGTCAAAGATACAGAGCGAACCTTAAGGTTGCTGATGACAGATAC
TACAAGTTTAACTCCAGAAAACCAACGGATAAAAGTTTAGGAAACGTGTTTGACTTGGGC
TGGAGAGAAAATTTCAAGCAAGTCATGGGCAATTCCTGGTATGAATGGCTGCTGCCAATA
AGGGTGGTACCTAAACGTTTGAATGACTATTATCTGAACAATGGATTGAACTACCCAGTG
AACGAGCATGTTTACCAAAAACTTGTTCAGAACTCTGAACTTCAACAACGATTGTATGAA
GACATATTAGATTACAGGAGGAAGCAGAAGCAAGACCAGGAAGCGGATTTGGAGAATGAA
TATCAACAATCCGGTACTGTCTATGGAGTTAGTGGTTTGGAGGTTTTGTTTCAAGGTCCA
AACGGTGCTCCATCGGCCGTG

Protein sequence predicted based on nucleotide sequence

MYYIKRCCCLLASTFPRAFCSGLFLWATYVLCIEIAFKLYGGIIGLVLCTVGVILLVISL
QAYCLVVALGPGSPADFTELHILNYVPGMQIKPPHELLNRTVTTSGSGNFRICNKCEVFK
PDRSHHCSSCGICILKMDHHCPWFACCIGFKNHKFFVQFLIYTQIYSLLALLVSGKVLYD
FFETKRYKDHYLSLNYVFLTVVSFVTFLSLTFFIGFTLYQLFRNKTTIESYESQRYRANL
KVADDRYYKFNSRKPTDKSLGNVFDLGWRENFKQVMGNSWYEWLLPIRVVPKRLNDYYLN
NGLNYPVNEHVYQKLVQNSELQQRLYEDILDYRRKQKQDQEADLENEYQQSGTVYGV

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  yeast    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
SolubleProtein: Not_Applicable
ProteinExpressed: Protein_Confirmed
ProteinPurified: Tested_Not_Purified
Find experimental protocols in MPSBC-Pfa3pPp
Recommended expression in:
Not Applicable