Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
PFA3-4_pSGP47
Keyword:
None
Species:
Pichia pastoris
Type:
cDNA
Vector Name:
pSGP47
Format:
FUSION
Source:
Membrane Protein Structural Biology Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Rochester
PSI:Biology
Membrane Protein Structural Biology Consortium
PSI:Biology
Membrane Protein Structural Biology Consortium
PpCD00585999
Price:
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 1071nts
Open reading frame: 40 to 1110
CAGGAATTTCCGACACCTCTCTTACACAAATATACCAAAATGTATTATATCAAAAGATGC
TGCTGCCTTCTGGCGTCCACATTCCCGAGGGCCTTTTGCAGCGGATTGTTCCTTTGGGCC
ACCTACGTACTGTGCATTGAGATTGCTTTCAAGTTATACGGCGGAATAATAGGGCTGGTT
CTTTGCACTGTTGGTGTAATTCTACTAGTCATCTCCTTGCAGGCGTACTGTTTGGTAGTT
GCCTTAGGTCCTGGATCTCCGGCTGACTTTACCGAGCTTCATATCTTAAACTACGTTCCT
GGGATGCAGATCAAACCACCCCACGAACTGCTAAACAGAACAGTTACCACCAGTGGAAGT
GGCAATTTCAGGATATGCAATAAGTGTGAAGTGTTCAAACCAGATAGAAGTCACCACTGC
TCTTCTTGTGGTATATGTATACTCAAAATGGACCATCATTGTCCTTGGTTTGCATGTTGC
ATTGGTTTCAAGAATCACAAATTTTTTGTTCAATTTCTGATCTATACACAGATATATTCC
TTATTAGCATTGCTGGTCAGTGGGAAGGTTCTTTACGATTTTTTTGAGACGAAGAGGTAT
AAGGATCATTATTTATCTCTGAACTATGTGTTCTTAACGGTTGTTAGTTTTGTGACTTTT
CTTTCGTTAACCTTTTTCATAGGGTTCACCCTGTACCAACTATTTCGTAACAAAACCACG
ATCGAATCCTACGAAAGTCAAAGATACAGAGCGAACCTTAAGGTTGCTGATGACAGATAC
TACAAGTTTAACTCCAGAAAACCAACGGATAAAAGTTTAGGAAACGTGTTTGACTTGGGC
TGGAGAGAAAATTTCAAGCAAGTCATGGGCAATTCCTGGTATGAATGGCTGCTGCCAATA
AGGGTGGTACCTAAACGTTTGAATGACTATTATCTGAACAATGGATTGAACTACCCAGTG
AACGAGCATGTTTACCAAAAACTTGTTCAGAACTCTGAACTTCAACAACGATTGTATGAA
GACATATTAGATTACAGGAGGAAGCAGAAGCAAGACCAGGAAGCGGATTTGGAGAATGAA
TATCAACAATCCGGTACTGTCTATGGAGTTAGTGGTTTGGAGGTTTTGTTTCAAGGTCCA
AACGGTGCTAGAGGTTCCCAT
Protein sequence predicted based on nucleotide sequence
MYYIKRCCCLLASTFPRAFCSGLFLWATYVLCIEIAFKLYGGIIGLVLCTVGVILLVISL QAYCLVVALGPGSPADFTELHILNYVPGMQIKPPHELLNRTVTTSGSGNFRICNKCEVFK PDRSHHCSSCGICILKMDHHCPWFACCIGFKNHKFFVQFLIYTQIYSLLALLVSGKVLYD FFETKRYKDHYLSLNYVFLTVVSFVTFLSLTFFIGFTLYQLFRNKTTIESYESQRYRANL KVADDRYYKFNSRKPTDKSLGNVFDLGWRENFKQVMGNSWYEWLLPIRVVPKRLNDYYLN NGLNYPVNEHVYQKLVQNSELQQRLYEDILDYRRKQKQDQEADLENEYQQSGTVYGV
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
yeast
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
SolubleProtein: Not_Applicable
ProteinExpressed: Protein_Confirmed
ProteinPurified: Tested_Not_Purified
Find experimental protocols in MPSBC-Pfa3pPp
Recommended expression in:
Not Applicable
Vector Name:
pSGP47
Synonyms:
''
Sequencing Primer:
Forward:
pSGP-Forward
Reverse:
pSGP-Reverse
Description:
Yeast expression vector with Adh2 promoter, adds 3C protease cleavage site and 10xHis tag; ampicillin resistance (bacteria); uracil (yeast); ligation independent cloning (LIC).
Comments:
S. cerevisiae expression strain
Size (bp):
6296
Parent Vector:
None
Properties:
ligation independent cloning (LIC), with tag/fusion/marker, yeast expression
Author Name:
Mark Dumont
Nadia Fedoriw
Center for High Throughput Structural Biology
PSI
Nadia Fedoriw
Center for High Throughput Structural Biology
PSI
Publications:
PMID:
20045057
Title: Purification of transmembrane proteins from Saccharomyces cerevisiae for X-ray crystallography
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
ligation independent cloning
LIC
Ligation independent cloning site
4220
4231
primer
primer
sequencing primer: CAATCAACTATCAACTATTAACTA
4155
4178
promoter
Ura3 promoter
Ura3 promoter
5981
6010
protease cleavage site
3C
Rhinovirus 3C protease cleavage site
4233
4256
selectable marker
AmpR
ampicillan selectable marker
1742
2401
selectable marker
Ura3
URA3 nutritional marker
5092
5895
tag
His
10xHis tag
4275
4304
