Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
SKOST_Beta_pSGP49
Keyword:
None
Species:
Leucoraja erinacea
Type:
cDNA
Vector Name:
pSGP49
Format:
FUSION
Source:
Membrane Protein Structural Biology Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Rochester
PSI:Biology
Membrane Protein Structural Biology Consortium
PSI:Biology
Membrane Protein Structural Biology Consortium
LeCD00586001
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 546nts
Open reading frame: 1 to 546
ATGAGCGGTTTGTTGAAATACCTCTTTGGTTGCTTCATCCTGTGTCTGCTGTTGCAAGGC
AAGACACATATGACATCAGCAACAATCAGCAAACCACATGAGACAATTGACATCGAGAAG
CAGAACATGACTGGAGAACGGAACAGTACATTAGCTCAGCAGTTATCCTTTCCGATGGAA
GATCCCACTAACTGGAACTATGCGATATTGGCCCTTGCATTTGTGGTTCTGTTTCTGGCA
TTCCTCATCCTGGCACAAAACTCCAGAGCTAACAGGACACGAAAGATGAAGGCATTGAAC
GGAGCAGGGGGCAGGAATGAAACGGAGGCAGACTCGACACAGAAGGCAATGATGCAATAT
GTTGTTGAGGTTGACAATCTTGCTGAAACAGACCAGATGCTACAGAGCAAGCCAACGTAC
ATCTCCCTGAACCAGGTGGCACAGACCTCATCTCCAAAGGTCCTCCCAAAAGAAGGACAG
ATCCTGGTCGAATGGAAGGATGGAAACATTGGCTTCCTTTACACCGACAGCAAGGAAGAT
GATGTG
Protein sequence predicted based on nucleotide sequence
MSGLLKYLFGCFILCLLLQGKTHMTSATISKPHETIDIEKQNMTGERNSTLAQQLSFPME DPTNWNYAILALAFVVLFLAFLILAQNSRANRTRKMKALNGAGGRNETEADSTQKAMMQY VVEVDNLAETDQMLQSKPTYISLNQVAQTSSPKVLPKEGQILVEWKDGNIGFLYTDSKED DV
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
yeast
Marker:
LEU2
Host Type: bacterial Marker: ampicillin
Host Type: bacterial Marker: ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
SolubleProtein: Not_Applicable
ProteinExpressed: Protein_Confirmed
ProteinPurified: Tested_Not_Purified
Find experimental protocols in MPSBC-SKOST_Beta
Recommended expression in:
Not Applicable
Vector Name:
pSGP49
Synonyms:
''
Sequencing Primer:
Forward:
Unknown
Reverse:
Unknown
Description:
Yeast expression expression vector with ZZ 10xHis tag; ampicillin resistance in bacteria, LEU2 resistance in yeast
Comments:
None
Size (bp):
7125
Parent Vector:
None
Properties:
yeast expression
Author Name:
University of Rochester
Membrane Protein Structural Biology Consortium
PSI:Biology
Membrane Protein Structural Biology Consortium
PSI:Biology
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin
165
847
selectable marker
AmpR
Ampicillin resistance
945
1604
tag
His
ZZ-10xHis tag
6496
6525
