Gene:
Gene Symbol:
PFA5
Gene Name:
Pfa5p
Original Clone ID:
YDR459C_pSGP47
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pSGP47
Format:
FUSION
Source:
Membrane Protein Structural Biology Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Rochester
PSI:Biology
Membrane Protein Structural Biology Consortium
PSI:Biology
Membrane Protein Structural Biology Consortium
ScCD00586003
Price:
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 1122nts
Open reading frame: 39 to 1160
AGGAAATTCCGACACCTTTCTTAAACAAATATACCAAAATGGCTCTATCATGGAATATTC
GAATCAGGCGCAGGTCATGGTTTAGATTCATCTTACCGATTATCGTGCTCGGTTTACTGT
GCTACGGTACGTGGGCTTACTGCCATAAACTATGTTACGAGCAAGTCGACAAACGACTTC
GTCAGAAATCAGTTTCGGTTGGTCTTATTTGTGCGGTATGCTTCCTAGACGTAGTCGTGA
TTTTCATATGGCTTCAAATAGTTATCTTGGTCGGTCCAGGTACCCAACCGCACGTAGCAC
CTTTTTTGATCCTTCCTATAGCCTCTGAAGAGAAGACTAGCAACACTTCTCAAAACACTT
CAGTGGAATACGATGCTGTGGTTCCGCCTAAGTGTTATCAGTCTGATCCTCACGGATACC
CAATATGGTGCAGTGAATGTCAAAGTCTGAAAATGGAACGAACGCATCATTCTTCAGAAC
TAGGCCACTGCATTCCTCGCTTTGATCATTACTGCATGTGGATAGGGACTGTTATTGGTA
GAGACAATTATAGACTCTTTGTGCAGTTTGCTGCTTATTTTTCAACACTTTTACTTATCA
TGTGGGTGTCTATTTGCGTTTACATAAGAATTATTACTCAACACAATCATAATTATAGCC
CCAATTTAAACGCAAATATAATTTCAACGTTAGTGTTTGCCATCCTTGGATGGTTATTAA
CCGCAAGTTTGTTGGCATCGAGTATTTTTTACATGAGTCAAAACAAGACGTCTTTGGAGG
CTATCATAGATAGCAAAAGGAAAAAATTTGGAACAAGGAAGATATTTTGTTATTACTCGG
AAGCAAACAAGTTGAGGTTTGTGGTAGAGTTCGATAGATCTGAATTTCACTCTTTCTGGG
ACAAAAAGTCAATCTTAGCTAATATAAAAGACTTTATGGGTTCTAACATACTTATGTGGA
TAATTCCTTTAGGCAAGCCCTATACATCTCGATGCAAATCTGATGGCAAAAGCGGTTCAA
AAACAACGCTAGTGGAAATATTGGGTCCCTATGAAGAAACTCTTAGTGACTACACCATTC
AGGCCATTGAGGACAAGATATCAAGAGGAGAATATCTGGCCACATTGCGGGCTTCTGGTG
ACGATAGTGATCCTGCTTACAGTGGTTTGGAGGTTTTGTTTCAAGGTCCAAACGGTGCTA
GAGGTTCCCAT
Protein sequence predicted based on nucleotide sequence
MALSWNIRIRRRSWFRFILPIIVLGLLCYGTWAYCHKLCYEQVDKRLRQKSVSVGLICAV CFLDVVVIFIWLQIVILVGPGTQPHVAPFLILPIASEEKTSNTSQNTSVEYDAVVPPKCY QSDPHGYPIWCSECQSLKMERTHHSSELGHCIPRFDHYCMWIGTVIGRDNYRLFVQFAAY FSTLLLIMWVSICVYIRIITQHNHNYSPNLNANIISTLVFAILGWLLTASLLASSIFYMS QNKTSLEAIIDSKRKKFGTRKIFCYYSEANKLRFVVEFDRSEFHSFWDKKSILANIKDFM GSNILMWIIPLGKPYTSRCKSDGKSGSKTTLVEILGPYEETLSDYTIQAIEDKISRGEYL ATLRASGDDSDPAY
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
yeast
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
SolubleProtein: Not_Applicable
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
Find experimental protocols in MPSBC-Pfa5p
Recommended expression in:
Not Applicable
Vector Name:
pSGP47
Synonyms:
''
Sequencing Primer:
Forward:
pSGP-Forward
Reverse:
pSGP-Reverse
Description:
Yeast expression vector with Adh2 promoter, adds 3C protease cleavage site and 10xHis tag; ampicillin resistance (bacteria); uracil (yeast); ligation independent cloning (LIC).
Comments:
S. cerevisiae expression strain
Size (bp):
6296
Parent Vector:
None
Properties:
ligation independent cloning (LIC), with tag/fusion/marker, yeast expression
Author Name:
Mark Dumont
Nadia Fedoriw
Center for High Throughput Structural Biology
PSI
Nadia Fedoriw
Center for High Throughput Structural Biology
PSI
Publications:
PMID:
20045057
Title: Purification of transmembrane proteins from Saccharomyces cerevisiae for X-ray crystallography
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
ligation independent cloning
LIC
Ligation independent cloning site
4220
4231
primer
primer
sequencing primer: CAATCAACTATCAACTATTAACTA
4155
4178
promoter
Ura3 promoter
Ura3 promoter
5981
6010
protease cleavage site
3C
Rhinovirus 3C protease cleavage site
4233
4256
selectable marker
AmpR
ampicillan selectable marker
1742
2401
selectable marker
Ura3
URA3 nutritional marker
5092
5895
tag
His
10xHis tag
4275
4304
