VPS4 (S. cerevisiae ) in pET151-D-Topo (Bacterial Expression)

Explanation of Terms

Gene:
Gene Symbol:  VPS4
Gene Name:  AAA family ATPase VPS4
Original Clone ID:
WISP13-150
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pET151-D-Topo
Format: 
CLOSED
Source:
University of Utah
Description:
None
Comments:
pET151-D-Topo-Vps4p
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Utah
Wesley I. Sundquist
Christopher P. Hill
Malgorzata Gonciarz
ScCD00586084
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AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 1314nts
Open reading frame: 51 to 1364

GTCTCGATTCTACGGAAAACCTGTATTTTCAGGGAATTGATCCCTTCACCATGAGCACGG
GAGATTTTTTAACTAAGGGAATAGAACTGGTTCAAAAGGCCATTGACCTGGACACAGCCA
CGCAGTATGAGGAGGCGTATACAGCATACTATAACGGATTAGATTATTTGATGTTGGCTT
TGAAATACGAGAAAAACCCTAAGTCGAAGGATTTGATAAGAGCGAAGTTTACAGAGTATC
TAAACCGTGCAGAGCAGTTGAAAAAGCACTTGGAAAGTGAAGAGGCAAACGCGGCAAAGA
AATCTCCTAGCGCTGGTAGTGGTTCCAACGGTGGTAATAAGAAAATCTCTCAGGAAGAGG
GTGAGGATAATGGTGGAGAAGACAACAAAAAGCTAAGAGGTGCCTTATCGAGTGCTATTT
TGTCTGAGAAGCCCAATGTTAAGTGGGAAGACGTGGCTGGACTGGAAGGAGCCAAAGAGG
CCCTTAAAGAAGCCGTTATTTTACCTGTCAAGTTCCCACATTTGTTCAAAGGTAACCGTA
AGCCCACTTCGGGAATCTTATTGTACGGACCACCAGGTACAGGTAAATCATATTTGGCAA
AAGCAGTGGCCACCGAGGCCAATTCTACCTTTTTCAGTGTTAGTTCCAGTGATTTGGTTT
CTAAGTGGATGGGTGAATCTGAAAAACTTGTCAAACAGTTATTTGCGATGGCAAGAGAAA
ATAAACCTTCTATTATTTTTATAGATGAAGTGGATGCGCTAACAGGTACCAGAGGAGAAG
GAGAGAGCGAAGCAAGTAGAAGAATTAAAACAGAATTATTGGTACAAATGAATGGTGTTG
GGAATGACTCCCAAGGTGTTTTGGTCCTTGGCGCAACAAATATCCCATGGCAACTGGACA
GTGCCATCAGGAGAAGATTTGAAAGAAGAATATATATACCATTACCAGATTTGGCGGCAA
GAACCACCATGTTCGAGATTAACGTCGGCGACACTCCATGCGTATTGACCAAGGAAGATT
ATAGAACTTTAGGTGCAATGACTGAAGGTTATTCCGGGAGTGATATTGCTGTAGTGGTGA
AAGATGCGCTAATGCAACCGATAAGAAAAATTCAAAGCGCGACACATTTCAAAGATGTCT
CTACGGAGGACGACGAAACAAGAAAACTAACACCATGCTCTCCAGGTGATGATGGCGCCA
TCGAGATGAGTTGGACGGATATTGAAGCTGATGAATTAAAAGAACCAGATCTCACCATAA
AGGATTTCTTAAAGGCTATCAAATCGACGAGACCTACCGTGAATGAGGATGACTTGCTGA
AGCAAGAACAGTTCACTAGAGATTTTGGTCAAGAAGGTAACTAG

Protein sequence predicted based on nucleotide sequence

MSTGDFLTKGIELVQKAIDLDTATQYEEAYTAYYNGLDYLMLALKYEKNPKSKDLIRAKF
TEYLNRAEQLKKHLESEEANAAKKSPSAGSGSNGGNKKISQEEGEDNGGEDNKKLRGALS
SAILSEKPNVKWEDVAGLEGAKEALKEAVILPVKFPHLFKGNRKPTSGILLYGPPGTGKS
YLAKAVATEANSTFFSVSSSDLVSKWMGESEKLVKQLFAMARENKPSIIFIDEVDALTGT
RGEGESEASRRIKTELLVQMNGVGNDSQGVLVLGATNIPWQLDSAIRRRFERRIYIPLPD
LAARTTMFEINVGDTPCVLTKEDYRTLGAMTEGYSGSDIAVVVKDALMQPIRKIQSATHF
KDVSTEDDETRKLTPCSPGDDGAIEMSWTDIEADELKEPDLTIKDFLKAIKSTRPTVNED
DLLKQEQFTRDFGQEGN*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing of cDNA insert
Mutations:
None
Discrepancy:
t94g,S32A
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Expressed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Recommended expression in:
Not Applicable