Gene:
Gene Symbol:
Ahos_1335
Gene Name:
cell division protein CdvC, Vps4
Original Clone ID:
WISP11-484
Keyword:
None
Species:
Acidianus hospitalis W1
Type:
cDNA
Vector Name:
pJExpress414-His-PP
Format:
CLOSED
Source:
University of Utah
Description:
None
Comments:
pJExpress414-AhosVps4
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Utah
Wesley I. Sundquist
DNA2.0
Wesley I. Sundquist
DNA2.0
AhCD00586085
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AVAILABLE
No restriction
Special MTA: SPTA
Special MTA: SPTA
Insert sequence: 1098nts
Open reading frame: 1 to 1098
ATGCTGGAGGAAATGGCCCGCAAGTACGCTATCGCAGCGGTTCGCGCGGACAAAGAGGGC
AACCGTGATGAAGCGATCACCTATTACAAAAAGGCGATTGACGTTCTGACCCAGATCGTG
GTGCTGTACCCGGATAGCCCGACGCGTCAAGCGTATGAGCAGATGATTGATGAGTATAAG
AAGCGTGTCTCGGTTCTGGAAAACATGCTGCCGGAAACCCCGCAAGAGGATAATAGCCAG
AAAACGGACGACGAGCTGATCATGAAGGAGAAGCCGAAAGTTAGCTTTAGCGATATTGTC
GGCCTGGACGACGTTAAAGAGGCGCTGAAAGAGGCGATCATCTATCCAAGCAAACGTCCG
GATCTGTTTCCACTGGGCTGGCCGCGTGGTATCCTGTTGTACGGTCCTCCGGGCAACGGT
AAAACCATGCTGGCGGCAGCTGTCGCCAACGAAATCGACTCTTACTTTATTCACGTCGAT
GCCGCTAGCATTATGAGCAAGTGGCTGGGCGAGGCCGAAAAGAATGTTGCGAAGATTTTC
AATACGGCTCGTGAATACTCTAAAAAGGACAATAAGCCTGCAATCATTTTTGTGGATGAG
ATCGATGCACTGCTGGGTACCTACACTAGCGAGGTTGGTGGTGAAGTCCGTGTGCGCAAT
CAATTCCTGAAAGAGATGGACGGTATTATGGACAAAAACGAGAACTATATGGTCTACGTG
ATTGGCGCGACTAACAAACCGTGGCGCCTGGATGAGCCGTTCCTGCGCCGTTTTCAAAAA
CGCATCTATGTGCCGCTGCCGGACTTCAATCAGCGTCTGGCACTGTTCAAATACTATACC
TCCAAAGTGAAGTTGGGTAACGTGGACCTGCAAGAGCTGGCGAAAATGACCGAAGGTTAT
TCCGCGAGCGATATCCGCGACATTGTACAGAGCGCACACATGCGTGTGGTTAAGGAGATG
TTCGAGAAGAACTTGTCCGAACCGCGTGAAATTACGATGGATGACTTTAAGGAAGTGCTG
AAAATTCGTAAGCCGAGCGTCAATCAGGAACTGTTGAAAGCATACGAAGCGTGGACCGAG
AAGTTCAAAGCCTTGTAA
Protein sequence predicted based on nucleotide sequence
MLEEMARKYAIAAVRADKEGNRDEAITYYKKAIDVLTQIVVLYPDSPTRQAYEQMIDEYK KRVSVLENMLPETPQEDNSQKTDDELIMKEKPKVSFSDIVGLDDVKEALKEAIIYPSKRP DLFPLGWPRGILLYGPPGNGKTMLAAAVANEIDSYFIHVDAASIMSKWLGEAEKNVAKIF NTAREYSKKDNKPAIIFVDEIDALLGTYTSEVGGEVRVRNQFLKEMDGIMDKNENYMVYV IGATNKPWRLDEPFLRRFQKRIYVPLPDFNQRLALFKYYTSKVKLGNVDLQELAKMTEGY SASDIRDIVQSAHMRVVKEMFEKNLSEPREITMDDFKEVLKIRKPSVNQELLKAYEAWTE KFKAL*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
End read sequencing of cDNA insert
Mutations:
None
Discrepancy:
a671n
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Expressed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Recommended expression in:
Not Applicable
Vector Name:
pJExpress414-His-PP
Synonyms:
''
Sequencing Primer:
Forward:
Unknown
Reverse:
Unknown
Description:
Bacterial expression vector with T7 promoter and 3C prescission protease-cleavable N-terminal 6xHis V5 tag; ampicillin resistance in bacteria; restriction enzyme cloning
Comments:
None
Size (bp):
4079
Parent Vector:
None
Properties:
bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
DNA2.0
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
promoter
T7
T7 promoter
1423
1442
protease cleavage site
3C
3C prescission protease cleavage site
1572
1595
repressor binding site
lac operator
lac operator
1442
1464
selectable marker
AmpR
Ampicillin resistance
2252
2911
tag
His
N-terminal 6xHis tag
1512
1529
tag
V5
N-terminal V5 tag
1530
1571
trxn termination sequence
T7 term
T7 terminator
1607
1653
