Kfla_4677 (K. flavida DSM 17836 )
In pMCSG68 (Bacterial Expression)

Explanation of Terms

Gene:
Gene Symbol:  Kfla_4677
Gene Name:  LacI family transcriptional regulator
Original Clone ID:
APC103529.103
Keyword:
None
Species:
Kribbella flavida DSM 17836
Type:
cDNA
Vector Name:
pMCSG68
Format: 
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
Argonne National Laboratory
Michael Endres
PSI:Biology
Midwest Center for Structural Genomics
KfCD00586243
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1020nts
Open reading frame: 51 to 1070

ATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCGTGCGGGTCA
CGCTGAAGGACATCGCCGACGAGGCCGGGGTCAGCATGATGACGGTGTCGAACGTGGTGA
ACGGCAAGCGGGCGCGGGTCCCCCCGGAGACGATCGAGCGGATCCAGCGCATCGTCGCCG
AGCGCGGCTACGTGCCGAGCGCGTCGGCGCGCAGCCTGGCGGCGAAGTCGTCGAAGCTGA
TCGGCCTGCTGGTGCCGGCCGCCGACGAGGACAGCCTGATGATCAGCCCGCACAACGTCG
CGATCGTCGGCCTGATCGAGCGCGAGCTGCGCAAGGGCGGCTACCACCTGCTGCTGCGCG
GGATCTCCCAGCCGGCCGAGGTGGCCGAGGCGCTGCAGTCGTGGAGCCTGGACGGCGCGG
TCCTGCTCGGCTTCCTCGACGAGGAGATCGACCGGCTGACCGCGAGGACCGTCGGCAAGG
TGTCGCTGCTGGCGATCGACAGCTACTCGGCGAACCCGCTGACCACCGGTGTACGGTCCG
ACGACAACACCGGCGCCCTGCTCGCTGCCCGGCACCTGCTGGCCCTCGGGCACCGCGAGA
TCGTGTTCGCGGGACCGGCCTTCTCCGATGTCGGCGTGGTGCACCAGCGCTACCAGGGCT
TCCGCGCCGCGTACGCCGAGGCGGGACTGACCTGGATCGAGCGGATCGTCACCGTCGAGA
CGACCACGCACGGCAGCGGCCGCGACCTCGGTCTGAGGCTGATGCAGTCCCACCCGGAGG
CGACCGCCGTCTTCGCCACGGCCGACATCCTGGCGATCGGCATCATGGAAGGGCTCGCCG
AGGCGGGCTGCTCGGTGCCCGGCGACGTGTCCGTGCTCGGCTTCGACGATCTCGACCTGA
GCCGTTACGTCACACCGAAGCTGACCACGATCGCGCAGGACATCCCGCAGAAGGCGGCGA
TCGCGGTCCGGTTGCTGCTGGCCGCCGTCGAGCGCCGCGAGCATCCCGCCGACCCGATCA
CCCTGGGCGTCCGCCTCATCACCCGCGACTCGACCGCCGCGCCGCGCTAACATTGGAAGT
GGATAATAA

Protein sequence predicted based on nucleotide sequence

VRVTLKDIADEAGVSMMTVSNVVNGKRARVPPETIERIQRIVAERGYVPSASARSLAAKS
SKLIGLLVPAADEDSLMISPHNVAIVGLIERELRKGGYHLLLRGISQPAEVAEALQSWSL
DGAVLLGFLDEEIDRLTARTVGKVSLLAIDSYSANPLTTGVRSDDNTGALLAARHLLALG
HREIVFAGPAFSDVGVVHQRYQGFRAAYAEAGLTWIERIVTVETTTHGSGRDLGLRLMQS
HPEATAVFATADILAIGIMEGLAEAGCSVPGDVSVLGFDDLDLSRYVTPKLTTIAQDIPQ
KAAIAVRLLLAAVERREHPADPITLGVRLITRDSTAAPR*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
t91c,S31P;c496a,H166N
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in MCSG-APC103529
Recommended expression in:
Not Applicable