Gene:
Gene Symbol:
Sked_25060
Gene Name:
metal-dependent hydrolase
Original Clone ID:
APC103688.102
Keyword:
None
Species:
Sanguibacter keddieii DSM 10542
Type:
cDNA
Vector Name:
pMCSG68
Format:
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Argonne National Laboratory
Michael Endres
PSI:Biology
Midwest Center for Structural Genomics
Michael Endres
PSI:Biology
Midwest Center for Structural Genomics
SkCD00586414
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 804nts
Open reading frame: 51 to 854
ATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGAAGCTGA
CGATCCTCGGGATGGCAGGTTCCTTCCCGTCGCCGGAGTCCCCCGCGTCCTCGTACCTCG
TGCAGGCCACGGACGCGGACGGCAGGACGTGGTCGGTCCTCATGGACCTGGGCAGCGGTG
CCCTCGGCGCGCTCCAGCGCGTCGCCAGCCCCTTCGACGTCGACGCGGTCGCGATCTCGC
ACATGCACCCCGACCACTTCGCGGACCTCTGCGGCTTCTACGTCTACCTCAAGTACCACC
CGGTGTGCGGCTCCGAGCGCGGGACCGGCCGTGCACCGACGCCGGTCTACGCCCCGGCAG
CGGCCGAGCAGCGCCTCGCCGAGGCCTACGGCCTCGAGGCCGAGGAGACCATGCGGGGTC
AGCTGGACTTCCGCTCGTGGGAGCCCGGGACGACCACGACGGTGGGCCCCTTCGAGATCG
AGCCCGTCGCGGTCTTCCACCCGGTGCCCGCCTATGGCGTCCGGGTCACCGGACCCAGCT
CGGTCGAGCCCGGTCGTCGCGTCACCCTCGCCTACACGGGGGACACCGACGCCTGCGACG
GCGTCGTCGACCTCGCTCGCTCCGCCGACCTGCTGCTCGCGGAGGCGGCCTTCGTCGAGG
GGCGCGACAGCGTGCGCGGCGTGCACCTCACGGGGCTGCGGGCCGGTGAGGTCGCGACCC
AGGCGGGTGCGGCCCGGCTGCTCCTCACGCACGTCCCGTCCTGGAACGACCCGGCCGTCA
CCGTCGCCGAGGCGGTCTCTGCCTACGACGGCCCCGTCGAGGTCGTGTCGCCGGGTGCGA
CCTACGAGCTCTAACATTGGAAGTGGATAATAA
Protein sequence predicted based on nucleotide sequence
MKLTILGMAGSFPSPESPASSYLVQATDADGRTWSVLMDLGSGALGALQRVASPFDVDAV AISHMHPDHFADLCGFYVYLKYHPVCGSERGTGRAPTPVYAPAAAEQRLAEAYGLEAEET MRGQLDFRSWEPGTTTTVGPFEIEPVAVFHPVPAYGVRVTGPSSVEPGRRVTLAYTGDTD ACDGVVDLARSADLLLAEAAFVEGRDSVRGVHLTGLRAGEVATQAGAARLLLTHVPSWND PAVTVAEAVSAYDGPVEVVSPGATYEL*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in MCSG-APC103688
Recommended expression in:
Not Applicable
Vector Name:
pMCSG68
Synonyms:
''
Sequencing Primer:
Forward:
T7short
Reverse:
T7 terminator
Description:
Bacterial expression vector with a T7 promoter and TEV-cleavable N-terminal 6xHis and StrepII tags; rare argU and ileX codons; ampicillin resistance in bacteria; ligation independent cloning
Comments:
BglI-XbaI fragment removed from pMCSG57 and ligated into pMCSG53 digested with the same enzymes
Size (bp):
4832
Parent Vector:
None
Properties:
bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
PSI:Biology
Midwest Center for Structural Genomics
PSI:Biology
Midwest Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
pBR322 origin
pBR322 origin of replication
2825
3413
ligation independent cloning
LIC
ligation independent cloning site
204
235
promoter
T7
T7 promoter
384
400
protease cleavage site
TEV
TEV protease cleavage site
223
243
repressor protein gene
LacI
Lac I gene
1437
2396
selectable marker
AmpR
ampicillin resistance gene
3584
4444
tRNA
ileX
ileX tRNA gene
983
1134
tRNA
argU
argU tRNA gene
467
853
tag
strep
N-terminal strepII tag
250
273
tag
His
N-terminal 6xHis tag
292
309
trxn regulatory element
lac operator
lac operator sequence
359
381
trxn termination sequence
T7 term
T7 terminator
26
72
