Dhaf_0025 (D. hafniense DCB-2 ) in pMCSG73 (Bacterial Expression)

Explanation of Terms

Gene:
Gene Symbol:  Dhaf_0025
Gene Name:  FMN-binding protein
Original Clone ID:
APC110485.103
Keyword:
None
Species:
Desulfitobacterium hafniense DCB-2
Type:
cDNA
Vector Name:
pMCSG73
Format: 
FUSION
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Argonne National Laboratory
Michael Endres
PSI:Biology
Midwest Center for Structural Genomics
DhCD00586713
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1473nts
Open reading frame: 4 to 1476

GCCTCAGCCGCCAACCAACCGGCGGAAAACCCACCGGCTGAAAAAGCAGTTGCAGCCGGG
ACTTACACGGCAAAAGCAGCCGGTTATCACGGAGATCTTGAAGTGACCACGGAAGTGGAT
CAGGAAGGAAAAATCACAAAGATTACCATAGGCGAAAATACGGAAACCGAGGATGTTGGC
GGCATCGCCATGGAGAAAATACCCCAGCGTATTATAGAAGCTCAATCCCTGGATGTGGAC
GTGATTGCCGGTGCGACACTGACCAGTAAGGGGATCATCAATGGGGTGGCCGCGGCCTTA
AAGGAGGCCGGTGCAGATCCTGTTCACTACGGTTATGTTGCCCCTGAAGAAGAGAGCGGG
GCGATCATGTCCCCTCTGAATAAGAGTGCCCTACCCGTAAAGAAAGAAACCACCGGTTCC
ATAACCATTAAGGATGTCAAAGGCCGGGAGGTAACCCTTGACCTGCCTATTTCCTCCTAT
GCTATCAGCACCATGGATGTCATCGACTATATTATACCGCTGAAAGGTGAAGAGGCTTTT
GCTATGCTGGTTGCTTCCGGACAGGACGGCGGTGGCGGTATCCAAAAATATGCTCAGCTG
TATAAGCCCATTGTCGGGGACTATACCCAACATTTAGGCCAGATTTCCGATCATAACGCG
CCTTTTGACCTGGAAATGGTTTTGTCCATGAATCCCGATGTCCTCATCGTCAATTCAGCT
ATGGCCGCTCATAAATATGCCTTGGAAATAGAGGAACAGCTTACGGCGGCCGGAATCAGC
ATTGTTCTCATCGATGTTCCCGGCAAGACATTGGATAAGTCGGTTCAGGAGACCATGAAG
CTCCTGGGCAAGATTTTCCAGGAGGGGGAAAAAGCCGCTGAAGTAGCAGCGTTCATAGAT
AAGCAGTATGAACTCCTTGCAGCCAAAAATCTTGCTCAGCGTCAGAATAAACCCAAGGTC
TATTATGAAAAGTCAGGATATTCCGAGGTCTACGGGAGCACGGGAACCAGCGTTTCCGGA
TGGGGTCTGCCGATTGCTATTGCCGGCGGCGACAATATTGCCGATGCCGTTCTTTTAGAT
AAAGCATCAGCCGGAGGAAGCAGCAATACCCTTGATCCTGAGTATGTCATTAAAGCCGAT
CCTGATTACATCATTCTCAGCGGAGTCAATGACGGCTGGCTGGACAGTCTTAAGCAAAAG
AAAGAACCTCCTCAGTATGATATTATCAACAGAACCGGCTGGAGCAATTTGCAGGCGGTT
AAAAACAACAATGTCTATGAATTTGCCCACTCAACCAGCCGCTCCATTTATGCTTTTTAC
CCTTGTCTGAAAATGGCCAAGCTCTTCTACCCTGAAGAATTTAAGGATCTGAATCCGGAA
GCGGTCCTTGATGAGTTTTTCGATCAATTTATGCTGGTGGGCAGCGATATCAGCACCTGG
TTTACGGGCCTTGAAGACCGTATTTCCCTAAAGAAA

Protein sequence predicted based on nucleotide sequence

SAANQPAENPPAEKAVAAGTYTAKAAGYHGDLEVTTEVDQEGKITKITIGENTETEDVGG
IAMEKIPQRIIEAQSLDVDVIAGATLTSKGIINGVAAALKEAGADPVHYGYVAPEEESGA
IMSPLNKSALPVKKETTGSITIKDVKGREVTLDLPISSYAISTMDVIDYIIPLKGEEAFA
MLVASGQDGGGGIQKYAQLYKPIVGDYTQHLGQISDHNAPFDLEMVLSMNPDVLIVNSAM
AAHKYALEIEEQLTAAGISIVLIDVPGKTLDKSVQETMKLLGKIFQEGEKAAEVAAFIDK
QYELLAAKNLAQRQNKPKVYYEKSGYSEVYGSTGTSVSGWGLPIAIAGGDNIADAVLLDK
ASAGGSSNTLDPEYVIKADPDYIILSGVNDGWLDSLKQKKEPPQYDIINRTGWSNLQAVK
NNNVYEFAHSTSRSIYAFYPCLKMAKLFYPEEFKDLNPEAVLDEFFDQFMLVGSDISTWF
TGLEDRISLKK

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in MCSG-APC110485
Recommended expression in:
Not Applicable