Taci_0145 (T. acidaminovorans DSM 6589 )
In pMCSG73 (Bacterial Expression)

Explanation of Terms

Gene:
Gene Symbol:  Taci_0145
Gene Name:  biotin and thiamin synthesis associated protein
Original Clone ID:
APC100796.104
Keyword:
None
Species:
Thermanaerovibrio acidaminovorans DSM 6589
Type:
cDNA
Vector Name:
pMCSG73
Format: 
FUSION
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
Argonne National Laboratory
PSI:Biology
Midwest Center for Structural Genomics
TaCD00586816
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1284nts
Open reading frame: 7 to 1290

AATGCCCTGGAGGCGGCCCGTTCCCGGGCCGGGGATGTGGGGTACCTGAGGGAAGTTTTG
GCCAAGGCCCGTCAGGCCAAGGGGCTGGACCCCCAGGAGGCGGCGGCACTCCTGTCGGTT
CAGGATCCCGCAATGGAGGATGAGATGTTCTCCCTGGCTAGGGAGGTAAAGGAGCGGATA
TACGGGCGCCGCATAGTGCTCTTCGCCCCCCTCTACGTGAGCAACCACTGTGTGAACGAC
TGTTCCTACTGCGGGTACAAATGCTCCAATCGGGGATTCGAGCGGCGGCGGCTGACCATG
GAGGAACTGGCGGAGGAGGTCCGGATCCTGGAGGGCCTGGGGCACAAGAGGCTGGCGTTG
GAGGCGGGGGAGGACCCGGTGAACTGCCCCTTGGACTACATCCTGGATTGCATAGGGACC
ATATACTCTCTCAAGTTCGACAACGGCAGCATAAGGAGGGTTAACGTCAACATAGCGGCC
ACCACGGTGGAGGAGTACCGGCGCCTCAAGGAGGCCGGCATAGGTACTTACGTTCTGTTC
CAGGAGACCTACCATCGACCCACCTACCGGGCGCTTCACCCTAGGGGGCCCAAGTCGTCG
TACGACTATCACACCACCGCCATGGACCGGGCCATGGAAGCCGGGATAGACGATGTGGGG
CTTGGGGTCCTCTACGGCCTGTACGACCACGTGTACGACACGGTGGCCATGCTGTTCCAC
GCCCGGCACCTGGAGGAGCGTTTCGGGGTGGGGCCCCACACCATATCGGTGCCAAGGCTC
AGGAGGGCCCACGGGGTGGACGTTTCCGCGTTCCAACTGGTGGACGACGAGGCCTTCAAA
CGCTTGGTGGCGGTCATAAGGCTTGCGGTGCCCTACACGGGGATGATCCTGTCCACCCGG
GAGGACGCGGAGTTCCGGGACCAGGTGATCTCCCTGGGGATATCCCAGATAAGCGCCGGT
TCCTGCACCGGGGTTGGGGGTTACGCGGTCCACTACGGCCGTTGGGCGGAGGACGAGAAG
CCCCAATTCGAGGTGGGGGATCACCGATCCCCCATGGAGATCATAAAGAGCCTCATGAGG
GGGGGGTACATACCCAGCTACTGCACCGCCTGCTACCGGGAGGGGCGCACCGGGGACCGG
TTCATGAGCCTGGCCAAGTCGGGGCAGATAGGCAACGTGTGCCAGCCCAACGCCCTGCTT
ACCTTGAAGGAGTTCCTCCTGGACTACGGTGATGAGGAGGCCAGGGAGATCGGAGAGCGG
CTCATCGAGAGGGAGGTCAATGAGATCCCC

Protein sequence predicted based on nucleotide sequence

LEAARSRAGDVGYLREVLAKARQAKGLDPQEAAALLSVQDPAMEDEMFSLAREVKERIYG
RRIVLFAPLYVSNHCVNDCSYCGYKCSNRGFERRRLTMEELAEEVRILEGLGHKRLALEA
GEDPVNCPLDYILDCIGTIYSLKFDNGSIRRVNVNIAATTVEEYRRLKEAGIGTYVLFQE
TYHRPTYRALHPRGPKSSYDYHTTAMDRAMEAGIDDVGLGVLYGLYDHVYDTVAMLFHAR
HLEERFGVGPHTISVPRLRRAHGVDVSAFQLVDDEAFKRLVAVIRLAVPYTGMILSTRED
AEFRDQVISLGISQISAGSCTGVGGYAVHYGRWAEDEKPQFEVGDHRSPMEIIKSLMRGG
YIPSYCTACYREGRTGDRFMSLAKSGQIGNVCQPNALLTLKEFLLDYGDEEAREIGERLI
EREVNEIP

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
a983t,D328V;c997t,R333W
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in MCSG-APC100796
Recommended expression in:
Not Applicable