TM0870 (T. maritima MSB8 ) in pMCSG73 (Bacterial Expression)

Explanation of Terms

Gene:
Gene Symbol:  TM0870
Gene Name:  penicillin-binding protein 2
Original Clone ID:
APC111275.103
Keyword:
None
Species:
Thermotoga maritima MSB8
Type:
cDNA
Vector Name:
pMCSG73
Format: 
FUSION
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Argonne National Laboratory
PSI:Biology
Midwest Center for Structural Genomics
TmCD00586835
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1686nts
Open reading frame: 5 to 1690

TGCCAATCTGTTCTTCTTTCCTCTGGCAGGGGAAAGAGAAAACTGGTTTGTTTCCATTCC
TCCTCGGAGAGGAAGTGTTCTGGATGTTCGGGGGAGGAAAATAGTTTATGATTCGCCAGA
ATACGTCGCTTATCTCGATGTTGACTTTTTTAAAAGGCAAAATGGGGATATAAAAGCACT
CGAGAAAACCTTACAAAACTGCGGTATCACAAAGTCAGCAGAGGAAGTAATGGAGTATAA
GTTTTTCAAACTCACAGAGGGAGAAGACAAAGTTGACGTGTTAAAGAAGATAGAATCAGA
GTTGCTGCCTTTTGTCAACATAGAACTGGTATACACCAGAAAAAAAATTCAAGATTACGC
CACAGGAATTCTGCTTGGAACGGTGATAGATGGAACGGGTAAGGGAGGAATAGAGGGTTT
TTTTGACGATCAGTTACAAGGAAAAAGAAAGGGTTTTTTAGAACTTCGATACAGAGGAGC
CCGCCTCTCTCCCACCCTTGTGAATTACTCCCCCCCCGAAAACGGAAAAGACGTCTGGCT
ATCTCTGGATCTTGACCTTCAAAGGCGCGTCTACGATATCATTTCGAAAGCCGTGGAAGG
AAACTCCGCAGAAGCTGGTCATGTGATTGTGATGGAATCGAAAACCGGCAGGATACTCTC
AATGGTGACCACCAGAAACTGGAACGATTTGATCGGTGGATACATAGAGCCAGGCTCGAC
GATAAAACCTCTGGTGTACGCGATCGCTCTCGAGACACATTCTGCTTCCCCAGATTTCAC
CGTTGAATGTGAAGGCTCAATAAAACCAGTAGAGCAACTCCCTGTGATCATAAGAGATAT
AGAAAAGCACGGTCTGGTGGATTTCTCCGCGGGAATCGTAAAATCGTGCAACGTGATGAG
TGTTAAGGTAGGAGAGCTCATTACAGAAAAAACCGGTGTTGAAGGATTCTATGAGTGGCT
CCGAAAGGTAGGTTTTGGGGAAAAAACCGGTATCGAAATGGAAGGGGAAATCGCTGGTGT
TCTGAGAGAACCGAAAAAATGGTCTCTCATAGATCCGGCTGAGATCTCGATTGGCCAGGG
TATCGGTGTCACTCCTGTACAGCTGATTGCGTCTCTCAATATCTTTGCGAACGATGGCTA
CTGGGTGAAACCTACCATTTTGAAAGATTCCCCAGTGAAAAAAAGAAGGGTCTTTTCAAA
AGAAACAACAGACGTGATAAGACAGGCGATGGTCCGGGTCGTGGAAGAAGGAACAGGAAA
ACTCGCTCAGGTGAAGGGAATAGCGATTGCTGGTAAAACAGGAACCGCGCAGAAGGCTGT
CGGTGGAGAATATAGGAATCTCTATCACTCACTTTTTGTGGGTTTTTTCCCCGCTGAAGA
TCCAAAGTACACGATACTGGTTCATCTGGACAGTCCCTCAGGGGCTTTCTACGGAGGAGA
GGTTGCCGCTCCGGTGTTCAGAGAGATCGTAGAGATTCTCACGGAGAAAGAAGACGGAAG
AATAAGGATTGTAAAAGGTCTCATGCCGGATCTGAGAGGTCTTCCAGTTAGAGACGCTTT
ACTCGTTCTGGAATCTCTCGGAGTGAAAGACGTGGAAATAAAAGGAAAAGGGTGGAAGGT
GTCTGAACAAACACCCCCACCCAATCATCCTCTTGAAGGACCTGTGATTCTGTTTTTGAG
CGATCAGAAG

Protein sequence predicted based on nucleotide sequence

NLFFFPLAGERENWFVSIPPRRGSVLDVRGRKIVYDSPEYVAYLDVDFFKRQNGDIKALE
KTLQNCGITKSAEEVMEYKFFKLTEGEDKVDVLKKIESELLPFVNIELVYTRKKIQDYAT
GILLGTVIDGTGKGGIEGFFDDQLQGKRKGFLELRYRGARLSPTLVNYSPPENGKDVWLS
LDLDLQRRVYDIISKAVEGNSAEAGHVIVMESKTGRILSMVTTRNWNDLIGGYIEPGSTI
KPLVYAIALETHSASPDFTVECEGSIKPVEQLPVIIRDIEKHGLVDFSAGIVKSCNVMSV
KVGELITEKTGVEGFYEWLRKVGFGEKTGIEMEGEIAGVLREPKKWSLIDPAEISIGQGI
GVTPVQLIASLNIFANDGYWVKPTILKDSPVKKRRVFSKETTDVIRQAMVRVVEEGTGKL
AQVKGIAIAGKTGTAQKAVGGEYRNLYHSLFVGFFPAEDPKYTILVHLDSPSGAFYGGEV
AAPVFREIVEILTEKEDGRIRIVKGLMPDLRGLPVRDALLVLESLGVKDVEIKGKGWKVS
EQTPPPNHPLEGPVILFLSDQK

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in MCSG-APC111275
Recommended expression in:
Not Applicable