mas (M. tuberculosis H37Rv ) in pMCSG73 (Bacterial Expression)

Explanation of Terms

Gene:
Gene Symbol:  mas
Gene Name:  Probable multifunctional mycocerosic acid synthase membrane-associated Mas
Original Clone ID:
APC112007.128
Keyword:
None
Species:
Mycobacterium tuberculosis H37Rv
Type:
cDNA
Vector Name:
pMCSG73
Format: 
FUSION
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
Argonne National Laboratory
PSI:Biology
Midwest Center for Structural Genomics
MtCD00587321
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1263nts
Open reading frame: 5 to 1267

TGCCACTCCCGTTGCGGTGATCGGGATGGGATGTCGGCTTCCTGGGGGGATCAACTCGCC
CGACAAACTGTGGGAGTCGTTGCTGCGCGGTGATGACTTGGTCACCGAGATTCCGCCCGA
CCGCTGGGACGCCGACGACTATTACGACCCTGAGCCAGGGGTTCCCGGGCGGTCGGTGTC
CCGGTGGGGTGGGTTCCTTGACGACGTCGCCGGTTTCGATGCTGAGTTCTTCGGGATTAG
CGAGCGGGAAGCGACCTCGATCGATCCGCAGCAGCGGCTACTGCTGGAAACGTCGTGGGA
GGCGATCGAGCATGCTGGTCTGGATCCGGCGTCGTTGGCCGGGTCCTCGACGGCCGTTTT
TACTGGGCTGACCCACGAGGACTACCTGGTACTCACCACAACGGCGGGCGGTTTGGCCAG
TCCATATGTGGTTACCGGCCTCAACAACAGTGTGGCGTCAGGGCGGATCGCGCACACATT
GGGTCTACATGGTCCGGCGATGACGTTTGACACCGCGTGTTCTTCGGGTCTGATGGCGGT
GCACCTGGCCTGCCGCAGCCTGCATGACGGCGAAGCTGACCTCGCTTTGGCGGGCGGTTG
TGCGGTGCTGCTGGAGCCGCATGCCAGTGTGGCGGCGTCTGCGCAGGGCATGCTGTCGTC
AACCGGTCGGTGCCATTCATTCGATGCTGACGCGGATGGGTTTGTGCGTTCCGAGGGCTG
CGCGATGGTGTTGCTGAAGCGACTGCCGGATGCGCTGCGCGACGGTAATCGGATCTTCGC
CGTGGTGCGTGGCACGGCCACCAATCAGGACGGCCGCACGGAGACGCTCACCATGCCGTC
CGAGGACGCCCAGGTTGCCGTGTACCGTGCGGCGTTGGCGGCGGCGGGCGTGCAGCCCGA
AACGGTCGGTGTGGTCGAGGCGCACGGCACCGGTACGCCAATCGGCGACCCGATTGAGTA
CCGCAGCCTGGCGCGGGTGTATGGCGCGGGCACCCCCTGCGCGCTTGGATCGGCCAAGAG
CAACATGGGGCACAGCACGGCATCGGCGGGGACCGTCGGGCTGATCAAGGCAATTCTGTC
ACTGCGGCACGGGGTGGTGCCGCCGCTGCTGCATTTCAATCGGCTGCCCGATGAGCTTTC
CGACGTCGAGACCGGGCTCTTTGTGCCGCAAGCGGTTACGCCGTGGCCCAACGGTAATGA
TCACACACTGAAGCGGGTCGCGGTGTCCTCGTTCGGGATGTCCGGGACCAACGTGCATGC
CATCGTG

Protein sequence predicted based on nucleotide sequence

TPVAVIGMGCRLPGGINSPDKLWESLLRGDDLVTEIPPDRWDADDYYDPEPGVPGRSVSR
WGGFLDDVAGFDAEFFGISEREATSIDPQQRLLLETSWEAIEHAGLDPASLAGSSTAVFT
GLTHEDYLVLTTTAGGLASPYVVTGLNNSVASGRIAHTLGLHGPAMTFDTACSSGLMAVH
LACRSLHDGEADLALAGGCAVLLEPHASVAASAQGMLSSTGRCHSFDADADGFVRSEGCA
MVLLKRLPDALRDGNRIFAVVRGTATNQDGRTETLTMPSEDAQVAVYRAALAAAGVQPET
VGVVEAHGTGTPIGDPIEYRSLARVYGAGTPCALGSAKSNMGHSTASAGTVGLIKAILSL
RHGVVPPLLHFNRLPDELSDVETGLFVPQAVTPWPNGNDHTLKRVAVSSFGMSGTNVHAI
V

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
c1205t,P402L
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in MCSG-APC112007
Recommended expression in:
Not Applicable