DNASU Plasmid | mas (M. tuberculosis H37Rv ) in pMCSG73 (Bacterial Expression)

Explanation of Terms

Gene:

Gene Symbol: mas

Gene Name: Probable multifunctional mycocerosic acid synthase membrane-associated Mas

Original Clone ID:

APC112007.131

Keyword:

None

Species:

Mycobacterium tuberculosis H37Rv

Type:

cDNA

Vector Name:

pMCSG73

Format:

FUSION

Source:

Midwest Center for Structural Genomics

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

Argonne National Laboratory
PSI:Biology
Midwest Center for Structural Genomics

MtCD00587322

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 885nts

Open reading frame: 6 to 890

ATGCCGTCTTCTCCGGGCAAGGGTCGCAGTGGGCGGCGATGGGCACGCAATTGCTCGCCA
GCGAACCAGTGTTCGCGGCCACCATCGCCAAGCTGGAGCCGGTGATCGCCGCAGAATCGG
GATTCTCGGTGACCGAGGCGATAACGGCGCAGCAGACCGTGACCGGAATCGACAAAGTGC
AGCCGGCAGTGTTCGCCGTTCAGGTCGCGTTGGCCGCCACCATGGAGCAAACCTACGGAG
TGCGGCCGGGCGCGGTCGTCGGACACTCGATGGGTGAGTCGGCCGCGGCCGTCGTCGCGG
GGGCACTGTCGCTCGAGGACGCGGCGCGCGTCATTTGCCGCCGCTCGAAGCTGATGACCC
GCATAGCCGGTGCTGGTGCCATGGGCTCGGTGGAATTGCCCGCCAAGCAAGTGAATTCGG
AGCTGATGGCACGCGGAATCGACGATGTTGTGGTCTCGGTGGTGGCGTCCCCGCAATCCA
CGGTGATCGGCGGTACGAGCGACACCGTTCGTGACCTCATCGCCCGTTGGGAGCAGCGGG
ACGTGATGGCGCGCGAGGTGGCCGTCGACGTGGCGTCGCACTCGCCTCAAGTCGATCCGA
TACTCGACGATTTGGCCGCGGCGCTGGCGGACATTGCTCCGATGACGCCCAAGGTGCCGT
ACTACTCGGCGACCCTGTTCGACCCGCGCGAGCAGCCGGTGTGCGATGGCGCTTACTGGG
TGGACAATCTGCGCAACACGGTGCAGTTCGCCGCGGCGGTGCAGGCTGCGATGGAGGACG
GCTACCGGGTCTTCGCGGAGCTGTCGCCCCACCCGCTGTTTACCCACGCCGTCGAACAGA
CGGGCCGAAGCCTCGACATGTCGGTCGCCGCCCTGGCCGGCATGCGGCGA

Protein sequence predicted based on nucleotide sequence

VFSGQGSQWAAMGTQLLASEPVFAATIAKLEPVIAAESGFSVTEAITAQQTVTGIDKVQP
AVFAVQVALAATMEQTYGVRPGAVVGHSMGESAAAVVAGALSLEDAARVICRRSKLMTRI
AGAGAMGSVELPAKQVNSELMARGIDDVVVSVVASPQSTVIGGTSDTVRDLIARWEQRDV
MAREVAVDVASHSPQVDPILDDLAAALADIAPMTPKVPYYSATLFDPREQPVCDGAYWVD
NLRNTVQFAAAVQAAMEDGYRVFAELSPHPLFTHAVEQTGRSLDMSVAALAGMRR

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

c814t,L272F

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinPurified: Not_Tested

ProteinExpressed: Protein_Confirmed

SolubleProtein: Tested_Not_Soluble

Find experimental protocols in MCSG-APC112007

Recommended expression in:

Not Applicable

Vector Name:

pMCSG73

Synonyms:

Sequencing Primer:

Forward: MCSG73F

Reverse: T7 terminator

Description:

Bacterial expression vector with a T7 promoter and TVMV-cleavable N-terminal NusA tag and TEV-cleavable N-terminal 6xHis and StrepII tag; Contains rate argU and ileX tRNA genes; ampicillin resistance in bacteria; ligation independent cloning.

Comments:

None

Size (bp):

6227

Parent Vector:

Name: pMCSG53

Description:

Properties:

bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker

Author Name:

Midwest Center for Structural Genomics
PSI:Biology
Midwest Center for Structural Genomics

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

ATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATGTTATCCACTTCCAATATTGGATTGGAAGTACAGGTTCTCCTTCTCGAACTGTGGATGAGACCAGTGATGATGATGGTGGTGAGACTGGAAACGCACGGTTTCGTTGTTGTTATTACGGGCAGCCATAATCAGTGCTCCGGCTTTTTCGTCGGTCAACCCTTCGATATCAGCCAGATCATCAATGCCCTGTTCGGCGAGATCTTCCAGCGTACAAACGCCACGGGCGGCCAGTTTGAATGCCAAATCACGATCTACCCCTTCAAGGTTCAGCAGATCGTCAGCCGGTTTGTTATCACCGAGGCTTTCTTCCTGGGCCTGTGCAATGGTGGCCAGTGCATTTTTAGCACGCTCGCGCAGTGCTTCAACGGTCGGCTCATCAAGGCCTTCGATTTCCAACAGCTCTTTCATCGGCACATAGGCCAATTCTTCCAGCGTCGAGAAGCCTTCTTCTACCAGAACAGTCGCGAAGTCTTCGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCTTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGTTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGATTACCGGCTTCAACGGCGATGTCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCGGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCCACAGGACGATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGGCAGCATATCTTCGCGCAGGATCACGGCTTCAGCGTTGTTGCCCAGATCCAGAGAGATGTTGTCGCGGTTTACTTTTTTCACCACGCCGGTGATGATTTCACCTTCGTGTTCACGGAACTGATCAACCACCATCGCACGTTCGGCTTCACGCACTTTCTGCACGATAACCTGTTTTGCCGTCTGGGTAGTGATACGGTCAAAGGTAACAGACTCAATCTGATCTTCAACGTAATCGCCCAGGTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGACTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCGCGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATATGTATATCTCCTTCTTAAAGTTAAACAAAATTATTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGTGGATTCAGTCAGGCGTCCCATTATCAGTGCTTCAGGAAATGGGCGGATGGGAGTCCATAGAAATGGTTCGTAGGTATGCTCACCTTGCGCCTAATCATTTGACAGAGCATGCGAGGAAAATAGACGACATTTTTGGTGATAATGTCCCAAATATGTCCCACTCTGGAATTATGGAGGATATAAAGAAGGCGTAACTGATTGAATTGTAATGGCGCGCCCTGCAGGATTCGAACCTGCGGCCCACGACTTAGAAGGTCGTTGCTCTATCCAACTGAGCTAAGGGCGCGTTGATACCGCAATGCGGTGTAATCGCGTGAATTATACGGTCAACCCTTGCTGAGTCAATGGCTTTTGATCTCGCCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCAAAAAGGCTGACGATTTCTCGTCAGCCTTTGCTATATCTGGTGGCCCCTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAGGGGCCATGGTAGCGGATTATAAAGTAACTCCGTGTCGCAATCCGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTACAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAAATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTAAATTGTAAGCGTTAATGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCCCATTCGCCA

Vector Features:

Type

Name

Description

Start

End

bacterial origin

pBR322 origin

pBR322 origin of replication

4220

4808

ligation independent cloning

LIC

ligation independent cloning site

138

promoter

T7 promoter

1779

1795

protease cleavage site

TVMV

TVMV protease cleavage site

218

238

protease cleavage site

TEV

TEV protease cleavage site

155

175

repressor binding site

lac operator

1754

1776

repressor protein gene

LacI

LacI CDS

2832

3791

selectable marker

AmpR

ampicillin resistance gene

4979

5839

tRNA

ileX

ileX tRNA gene

2378

2529

tRNA

argU

argU tRNA

1862

2218

tag

NusA

N-terminal NusA

249

1708

tag

His

N-terminal 6xHis tag

200

217

tag

strep

N-terminal StrepII tag

176

199

trxn termination sequence

T7 term

T7 terminator

Gene

Gene Symbol :

mas

GENEID :

887982

Locus Tag :

Rv2940c

GI :

15610077

Target GenBank:

None

Protein

SMART domain :

PKS_AT : Acyl transferase domain in polyketide synthase (PKS) enzymes.

Structural Annotation Wiki :

MCSG-APC112007

Clone

Cloning Information :

650502

Original Clone ID :

APC112007.131

Experimental

TargetTrack - Experimental data :

MCSG-APC112007

Organism

Species Specific ID:

887982