VPS4A (Homo sapiens) in pEGFP-C1

Explanation of Terms

Gene:
Gene Symbol:  VPS4A
Gene Name:  vacuolar protein sorting 4 homolog A (S. cerevisiae)
Original Clone ID:
WISP01-111
Keyword:
VPS4A aa 1-437
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pEGFP-C1
Format: 
FUSION
Source:
University of Utah
Description:
None
Comments:
Flanking regions are 5¿ TCAGATCTCGAGCTCAAGCTTCGAATTCTGCG and 3¿ GGATCCACCGGATCTAGATAACTGATCAT, note there is a mutation of the last nucleotide in the 5¿ sequence above, G replaces an A in the empty vector.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Utah
Uta von Schwedler
Wesley I. Sundquist
HsCD00587768
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AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 1314nts
Open reading frame: 1 to 1314

ATGACAACGTCAACCCTCCAGAAAGCCATTGATCTGGTGACGAAAGCCACAGAGGAGGAC
AAAGCCAAGAACTACGAGGAGGCGCTGCGGCTGTACCAGCATGCGGTGGAGTACTTCCTC
CACGCTATCAAGTATGAGGCCCACAGCGACAAGGCCAAGGAGAGCATTCGAGCCAAGTGC
GTGCAGTACCTAGACCGGGCCGAGAAGCTGAAGGATTATTTACGAAGCAAAGAGAAACAC
GGCAAGAAGCCAGTCAAAGAGAACCAGAGTGAGGGCAAGGGCAGTGACAGTGACAGTGAA
GGGGATAATCCGGAGAAAAAGAAACTGCAAGAACAGCTGATGGGTGCCGTCGTGATGGAG
AAGCCCAACATACGGTGGAACGACGTGGCCGGGCTGGAGGGGGCCAAGGAGGCCCTCAAA
GAAGCTGTCATTTTGCCAATCAAATTCCCACACTTGTTCACAGGCAAGCGCACCCCCTGG
CGGGGGATTCTGCTGTTCGGACCCCCTGGCACAGGGAAATCCTACCTGGCCAAAGCCGTG
GCAACAGAGGCCAACAACTCCACCTTCTTCTCTGTGTCCTCCTCAGATCTGATGTCCAAG
TGGCTGGGGGAGAGTGAAAAGCTGGTCAAGAACCTGTTTGAGCTGGCCAGGCAGCACAAG
CCCTCCATCATCTTCATCGATGAGGTGGATTCCCTCTGCGGGTCCCGAAATGAAAATGAG
AGTGAGGCCGCCCGGAGGATCAAAACGGAGTTCTTGGTCCAGATGCAGGGGGTGGGGAAT
AACAATGATGGGACTCTGGTTCTTGGAGCCACAAACATCCCATGGGTGTTGGATTCGGCC
ATCAGGAGGAGGTTTGAAAAACGAATTTATATCCCCTTGCCGGAGGAAGCTGCCCGCGCC
CAGATGTTCCGGTTGCATCTCGGGAGCACTCCCCACAACCTCACGGATGCAAACATCCAC
GAGCTGGCCCGGAAGACGGAAGGCTACTCGGGCGCGGACATCAGCATCATCGTGCGGGAC
TCTCTCATGCAGCCCGTGAGGAAGGTGCAGTCGGCCACACACTTCAAAAAGGTCTGTGGC
CCCTCTCGCACCAACCCCAGCATGATGATTGATGACCTCCTGACTCCATGCTCACCAGGG
GACCCAGGAGCCATGGAGATGACTTGGATGGATGTCCCTGGGGACAAACTCTTAGAGCCT
GTGGTTTGCATGTCGGACATGCTGCGGTCTCTGGCCACCACCCGGCCCACGGTGAATGCA
GACGACCTCCTGAAAGTGAAGAAATTCTCAGAGGACTTTGGGCAAGAGAGTTAA

Protein sequence predicted based on nucleotide sequence

MTTSTLQKAIDLVTKATEEDKAKNYEEALRLYQHAVEYFLHAIKYEAHSDKAKESIRAKC
VQYLDRAEKLKDYLRSKEKHGKKPVKENQSEGKGSDSDSEGDNPEKKKLQEQLMGAVVME
KPNIRWNDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAV
ATEANNSTFFSVSSSDLMSKWLGESEKLVKNLFELARQHKPSIIFIDEVDSLCGSRNENE
SEAARRIKTEFLVQMQGVGNNNDGTLVLGATNIPWVLDSAIRRRFEKRIYIPLPEEAARA
QMFRLHLGSTPHNLTDANIHELARKTEGYSGADISIIVRDSLMQPVRKVQSATHFKKVCG
PSRTNPSMMIDDLLTPCSPGDPGAMEMTWMDVPGDKLLEPVVCMSDMLRSLATTRPTVNA
DDLLKVKKFSEDFGQES*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing of cDNA insert
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable