DNASU Plasmid | NFYA (H. sapiens )<br>In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: NFYA

Gene Name: nuclear transcription factor Y, alpha

Original Clone ID:

HR6427-1-290-14.3

Keyword:

targeted domain

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pET15_NESG

Format:

CLOSED

Source:

Northeast Structural Genomics Consortium

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Rutgers University
PSI:Biology
Northeast Structural Genomics Consortium

HsCD00588953

Price:

AVAILABLE

No restriction
Special MTA: PSI/NESG

Insert Sequence
Protein Sequence

Insert sequence: 786nts

Open reading frame: 10 to 795

Reference Sequence Alignment

CACAGCCATATGGAGCAGTATACAGCAAACAGCAATAGTTCGACAGAGCAGATTGTTGTC
CAGGCAGGACAGATTCAGCAGCAGGTCCAAGGGCAGCCATTAATGGTGCAGGTCAGTGGA
GGCCAGCTAATCACATCAACTGGCCAACCCATCATGGTCCAGGCTGTCCCTGGTGGACAA
GGTCAAACCATCATGCAAGTACCTGTTTCTGGAACACAGGGTTTGCAGCAAATACAGTTG
GTCCCACCTGGACAGATCCAGATCCAGGGTGGACAGGCTGTGCAGGTGCAGGGCCAGCAG
GGCCAGACCCAGCAGATCATCATCCAGCAGCCCCAGACGGCTGTCACTGCTGGCCAGACT
CAGACACAGCAGCAGATTGCTGTCCAGGGACAGCAAGTGGCACAGACTGCTGAAGGGCAG
ACCATCGTCTATCAACCAGTTAATGCAGATGGCACCATTCTCCAGCAAGTTACAGTCCCT
GTTTCAGGCATGATCACTATCCCAGCAGCCAGTTTGGCAGGAGCACAGATTGTTCAAACA
GGAGCCAATACCAACACAACCAGCAGTGGGCAAGGGACTGTCACTGTGACACTACCAGTG
GCAGGCAATGTGGTCAATTCAGGAGGGATGGTCATGATGGTTCCTGGGGCTGGCTCTGTG
CCTGCTATCCAAAGAATCCCTCTACCTGGAGCAGAGATGCTTGAAGAAGAGCCTCTCTAC
GTGAATGCCAAACAATACCACCGTATTCTTAAGAGGAGGCAAGCCCGAGCTAAACTAGAG
GCAGAAGGGAAATAG

Protein sequence predicted based on nucleotide sequence

MEQYTANSNSSTEQIVVQAGQIQQQVQGQPLMVQVSGGQLITSTGQPIMVQAVPGGQGQT
IMQVPVSGTQGLQQIQLVPPGQIQIQGGQAVQVQGQQGQTQQIIIQQPQTAVTAGQTQTQ
QQIAVQGQQVAQTAEGQTIVYQPVNADGTILQQVTVPVSGMITIPAASLAGAQIVQTGAN
TNTTSSGQGTVTVTLPVAGNVVNSGGMVMMVPGAGSVPAIQRIPLPGAEMLEEEPLYVNA
KQYHRILKRRQARAKLEAEGK*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

del@76,87

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinPurified: Not_Tested

ProteinExpressed: Protein_Confirmed

SolubleProtein: Tested_Not_Soluble

Find experimental protocols in HR6427

Recommended expression in:

Not Applicable

Vector Name:

pET15_NESG

Synonyms:

pET15, pET15-modified, pET15Cc, pET15_NESG

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.

Comments:

Modified Novagen pET vector-common MCS

Size (bp):

5723

Parent Vector:

None

Properties:

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker

Author Name:

Gaetano T. Montleione
Jessica Bearden
Northeast Structural Genomics Consortium

Publications:

PMID: 15808222

Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:

Vector Sequence:

Sequence:

TTCTCATGTTTGACAGCTTATCATCGATAAGCTAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCGAGAAGCTTTGCGTCGACAGAGCTCGCAGGATCCGCAGAATTCGCCATATGGCTGTGATGGTGATGGTGATGGCCCATGGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAA

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI)

327

452

bacterial origin

ori

ColE1 origin of replication

3881

4563

gene fragment

ROP

repressor of primer ROP sequence

3281

3457

primer

T7 forward

T7 forward sequencing primer

481

496

primer

T7 reverse

T7 reverse sequencing primer

257

275

promoter

T7 promoter

478

497

repressor binding site

lacO

lacO regulatory sequence

456

478

selectable marker

AmpR

ampicillin resistance gene

4661

5230

tag

His

N-terminal 6x His tag

387

404

trxn termination sequence

T7 term

T7 termination sequence

257

275

Gene

Gene Symbol :

NFYA

Symbol Nomenclature :

NFYA

SYNONYM :

CBF-A; CBF-B; HAP2; NF-YA

Designation :

Full Nomenclature :

nuclear transcription factor Y, alpha

GENEID :

4800

GI :

11496974

GenBank Accession :

NM_021705

HGNC :

7804

MIM :

189903

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00588953      CACAGCCATATGGAGCAGTATACAGCAAACAGCAATAGTTCGACAGAGCAGATTGTTGTC
NM_002505.4       ---------ATGGAGCAGTATACAGCAAACAGCAATAGTTCGACAGAGCAGATTGTTGTC
NM_021705.3       ---------ATGGAGCAGTATACAGCAAACAGCAATAGTTCGACAGAGCAGATTGTTGTC
                           ***************************************************

HsCD00588953      CAGGCAGGACAGATTCAGCAGCAGGTCCAAG-----------------------------
NM_002505.4       CAGGCAGGACAGATTCAGCAGCAG----CAGCAGGGTGGTGTCACTGCTGTGCAGTTGCA
NM_021705.3       CAGGCAGGACAGATTCAGCAGCAG----GTCCAAG-------------------------
                  ************************     :                              

HsCD00588953      ------------------------------------------------------------
NM_002505.4       GACTGAGGCCCAGGTGGCATCCGCCTCAGGCCAGCAAGTCCAGACCCTCCAGGTAGTCCA
NM_021705.3       ------------------------------------------------------------
                                                                              

HsCD00588953      --GGCAGCCATTAATGGTGCAGGTCAGTGGAGGCCAGCTAATCACATCAACTGGCCAACC
NM_002505.4       AGGGCAGCCATTAATGGTGCAGGTCAGTGGAGGCCAGCTAATCACATCAACTGGCCAACC
NM_021705.3       --GGCAGCCATTAATGGTGCAGGTCAGTGGAGGCCAGCTAATCACATCAACTGGCCAACC
                    **********************************************************

HsCD00588953      CATCATGGTCCAGGCTGTCCCTGGTGGACAAGGTCAAACCATCATGCAAGTACCTGTTTC
NM_002505.4       CATCATGGTCCAGGCTGTCCCTGGTGGACAAGGTCAAACCATCATGCAAGTACCTGTTTC
NM_021705.3       CATCATGGTCCAGGCTGTCCCTGGTGGACAAGGTCAAACCATCATGCAAGTACCTGTTTC
                  ************************************************************

HsCD00588953      TGGAACACAGGGTTTGCAGCAAATACAGTTGGTCCCACCTGGACAGATCCAGATCCAGGG
NM_002505.4       TGGAACACAGGGTTTGCAGCAAATACAGTTGGTCCCACCTGGACAGATCCAGATCCAGGG
NM_021705.3       TGGAACACAGGGTTTGCAGCAAATACAGTTGGTCCCACCTGGACAGATCCAGATCCAGGG
                  ************************************************************

HsCD00588953      TGGACAGGCTGTGCAGGTGCAGGGCCAGCAGGGCCAGACCCAGCAGATCATCATCCAGCA
NM_002505.4       TGGACAGGCTGTGCAGGTGCAGGGCCAGCAGGGCCAGACCCAGCAGATCATCATCCAGCA
NM_021705.3       TGGACAGGCTGTGCAGGTGCAGGGCCAGCAGGGCCAGACCCAGCAGATCATCATCCAGCA
                  ************************************************************

HsCD00588953      GCCCCAGACGGCTGTCACTGCTGGCCAGACTCAGACACAGCAGCAGATTGCTGTCCAGGG
NM_002505.4       GCCCCAGACGGCTGTCACTGCTGGCCAGACTCAGACACAGCAGCAGATTGCTGTCCAGGG
NM_021705.3       GCCCCAGACGGCTGTCACTGCTGGCCAGACTCAGACACAGCAGCAGATTGCTGTCCAGGG
                  ************************************************************

HsCD00588953      ACAGCAAGTGGCACAGACTGCTGAAGGGCAGACCATCGTCTATCAACCAGTTAATGCAGA
NM_002505.4       ACAGCAAGTGGCACAGACTGCTGAAGGGCAGACCATCGTCTATCAACCAGTTAATGCAGA
NM_021705.3       ACAGCAAGTGGCACAGACTGCTGAAGGGCAGACCATCGTCTATCAACCAGTTAATGCAGA
                  ************************************************************

HsCD00588953      TGGCACCATTCTCCAGCAAGTTACAGTCCCTGTTTCAGGCATGATCACTATCCCAGCAGC
NM_002505.4       TGGCACCATTCTCCAGCAAGTTACAGTCCCTGTTTCAGGCATGATCACTATCCCAGCAGC
NM_021705.3       TGGCACCATTCTCCAGCAAGTTACAGTCCCTGTTTCAGGCATGATCACTATCCCAGCAGC
                  ************************************************************

HsCD00588953      CAGTTTGGCAGGAGCACAGATTGTTCAAACAGGAGCCAATACCAACACAACCAGCAGTGG
NM_002505.4       CAGTTTGGCAGGAGCACAGATTGTTCAAACAGGAGCCAATACCAACACAACCAGCAGTGG
NM_021705.3       CAGTTTGGCAGGAGCACAGATTGTTCAAACAGGAGCCAATACCAACACAACCAGCAGTGG
                  ************************************************************

HsCD00588953      GCAAGGGACTGTCACTGTGACACTACCAGTGGCAGGCAATGTGGTCAATTCAGGAGGGAT
NM_002505.4       GCAAGGGACTGTCACTGTGACACTACCAGTGGCAGGCAATGTGGTCAATTCAGGAGGGAT
NM_021705.3       GCAAGGGACTGTCACTGTGACACTACCAGTGGCAGGCAATGTGGTCAATTCAGGAGGGAT
                  ************************************************************

HsCD00588953      GGTCATGATGGTTCCTGGGGCTGGCTCTGTGCCTGCTATCCAAAGAATCCCTCTACCTGG
NM_002505.4       GGTCATGATGGTTCCTGGGGCTGGCTCTGTGCCTGCTATCCAAAGAATCCCTCTACCTGG
NM_021705.3       GGTCATGATGGTTCCTGGGGCTGGCTCTGTGCCTGCTATCCAAAGAATCCCTCTACCTGG
                  ************************************************************

HsCD00588953      AGCAGAGATGCTTGAAGAAGAGCCTCTCTACGTGAATGCCAAACAATACCACCGTATTCT
NM_002505.4       AGCAGAGATGCTTGAAGAAGAGCCTCTCTACGTGAATGCCAAACAATACCACCGTATTCT
NM_021705.3       AGCAGAGATGCTTGAAGAAGAGCCTCTCTACGTGAATGCCAAACAATACCACCGTATTCT
                  ************************************************************

HsCD00588953      TAAGAGGAGGCAAGCCCGAGCTAAACTAGAGGCAGAAGGGAAATAG--------------
NM_002505.4       TAAGAGGAGGCAAGCCCGAGCTAAACTAGAGGCAGAAGGGAAAATTCCAAAGGAGAGAAG
NM_021705.3       TAAGAGGAGGCAAGCCCGAGCTAAACTAGAGGCAGAAGGGAAAATTCCAAAGGAGAGAAG
                  *******************************************::               

HsCD00588953      ------------------------------------------------------------
NM_002505.4       GAAATACCTGCATGAGTCTCGGCACCGTCATGCCATGGCACGGAAGCGTGGTGAAGGTGG
NM_021705.3       GAAATACCTGCATGAGTCTCGGCACCGTCATGCCATGGCACGGAAGCGTGGTGAAGGTGG
                                                                              

HsCD00588953      ------------------------------------------------------------
NM_002505.4       ACGATTTTTCTCTCCAAAGGAAAAGGATAGTCCCCATATGCAGGATCCAAACCAAGCCGA
NM_021705.3       ACGATTTTTCTCTCCAAAGGAAAAGGATAGTCCCCATATGCAGGATCCAAACCAAGCCGA
                                                                              

HsCD00588953      -------------------------------------
NM_002505.4       TGAAGAAGCAATGACACAGATCATCCGAGTGTCCTAA
NM_021705.3       TGAAGAAGCAATGACACAGATCATCCGAGTGTCCTAA

Pathways

NCI :

Direct p53 effectors

NCI :

Validated targets of C-MYC transcriptional repression

Reactome :

Activation of gene expression by SREBF (SREBP)

Reactome :

ATF4 activates genes

Reactome :

ATF6-alpha activates chaperone genes

Reactome :

ATF6-alpha activates chaperones

Reactome :

Fatty acid, triacylglycerol, and ketone body metabolism

Reactome :

Metabolism

Reactome :

Metabolism of lipids and lipoproteins

Reactome :

Metabolism of proteins

Reactome :

PERK regulates gene expression

Reactome :

PPARA activates gene expression

Reactome :

Regulation of cholesterol biosynthesis by SREBP (SREBF)

Reactome :

Regulation of lipid metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha)

Reactome :

Unfolded Protein Response (UPR)

WikiPathway :

SIDS Susceptibility Pathways

WikiPathway :

SREBP signalling

WikiPathway :

Wnt Signaling Pathway and Pluripotency

Protein

SMART domain :

CBF : CCAAT-Binding transcription Factor

Structural Annotation Wiki :

HR6427

UniProt :

P23511

HPRD :

01793

Clone

Cloning Information :

627469

Original Clone ID :

HR6427-1-290-14.3

Experimental

TargetTrack - Experimental data :

HR6427

Organism

Species Specific ID:

4800

Genome

Chromosome :

Map Location :

6p21.3

Reagents

Labome :

NF-YA-antibody

NFYA (H. sapiens )In pET15_NESG (His-tagged bacterial expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Pathways

Protein

Clone

Experimental

Organism

Genome

Reagents

NFYA (H. sapiens )
In pET15_NESG (His-tagged bacterial expression vector)