BUB1 (H. sapiens )
In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  BUB1
Gene Name:  BUB1 mitotic checkpoint serine/threonine kinase
Original Clone ID:
HR5460A-1-181-14.12
Keyword:
targeted domain
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pET15_NESG
Format: 
CLOSED
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
Rutgers University
PSI:Biology
Northeast Structural Genomics Consortium
HsCD00589011
Price: 
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AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 546nts
Open reading frame: 51 to 596

TTTAAGAAGGAGATATACCATGGGGCCATCACCATCACCATCACAGCCATATGGACACCC
CGGAAAATGTCCTTCAGATGCTTGAAGCCCACATGCAGAGCTACAAGGGCAATGACCCTC
TTGGTGAATGGGAAAGATACATACAGTGGGTAGAAGAGAATTTTCCTGAGAATAAAGAAT
ACTTGATAACTTTACTAGAACATTTAATGAAGGAATTTTTAGATAAGAAGAAATACCACA
ATGACCCAAGATTCATCAGTTATTGTTTAAAATTTGCTGAGTACAACAGTGACCTCCATC
AATTTTTTGAGTTTCTGTACAACCATGGGATTGGAACCCTGTCATCCCCTCTGTACATTG
CCTGGGCGGGGCATCTGGAAGCCCAAGGAGAGCTGCAGCATGCCAGTGCTGTCCTTCAGA
GAGGAATTCAAAACCAGGCTGAACCCAGAGAGTTCCTGCAACAACAATACAGGTTATTTC
AGACACGCCTCACTGAAACCCATTTGCTAGCTCAAGCTAGAACCTCAGAACCTCTGCATA
ATGTTCAGGTTTTAAATCAAATGATAACATCAAAATCAAATCCAGGAAATAACTAGCTCG
AGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTG

Protein sequence predicted based on nucleotide sequence

MDTPENVLQMLEAHMQSYKGNDPLGEWERYIQWVEENFPENKEYLITLLEHLMKEFLDKK
KYHNDPRFISYCLKFAEYNSDLHQFFEFLYNHGIGTLSSPLYIAWAGHLEAQGELQHASA
VLQRGIQNQAEPREFLQQQYRLFQTRLTETHLLAQARTSEPLHNVQVLNQMITSKSNPGN
N*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
c458t,P153L
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in HR5460A
Recommended expression in:
Not Applicable