MA3217 (M. acetivorans )
In pET21_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  MA3217
Gene Name:  cysteine protease
Original Clone ID:
MvR97A-29-340-21.92
Keyword:
full length protein truncation
Species:
Methanosarcina acetivorans
Type:
cDNA
Vector Name:
pET21_NESG
Format: 
FUSION
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Rutgers University
PSI:Biology
Northeast Structural Genomics Consortium
MaCD00589055
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 939nts
Open reading frame: 31 to 969

TTTTGTTTAACTTTAGAAGGAGATATACATATGCCGTCCTTAAAAGATTACACAGAAGAA
AGCGCTCAGATTCCCGAGATGGCAGAAAAACTCGGAATTCCGACTTCCAGAAAGGAAACA
AAAACTCTGGAACTGCCTGTTTCCATAGACTTCAGGAATTGGTGTTCGCCGATTGAAGAT
CAAATGGATCTGGGCTCCTGTACCGCCCACGCAGGTGTAGGAGTGGTAGAGTACTTTGAG
CGACGAGCCTTTGGGAAACATATTGACGGGTCAAGATTTTTTGTCTACAAGACGACCAGA
AACCTGATGGGAGTCAAGGGAGACACCGGAGCCTGGCTCCGGAACACCATGGGAGCACTG
GCTCTTTGCGGTGTCCCGCCTGAAAAGTATTTTCCCTACACCAGCAGGAAAACTCCGGGA
CCGGACGGAGAACCGACATTTGACGACGAACCTTCAAGCTTTGTATATTCGCTTGCCGAC
AACTTCGAAGCCCTTAACTACTTCTGCCATGACCCGCAGGGCATGAATTTACCACCTGAA
GATGTCATCCTCAGCGTGAAGAAGTACCTTGCGGCAGGAATTCCCTCAATGTTCGGCTTT
TACGGGTTTCCCTCCTTTAAGGATACAGATGTTAAAGGTGGAATTCCTTTCCCGGGTCCC
GATGAGAGGTCAATATGGGGCCACGCGGTCGTTGCAGTCGGCTATGATGATAACATGAAG
ATCAGAAATACCCTCAGCAACAAAGAAACCACCGGTGCCTTTTTGATCCGCAATTCCTGG
GGCACAGCCTGGGGTGATAAGGGGTATGGATGGCTGCCTTATGAATATGCCCTGAACAAA
TTTGCACTGGACTTCTGGTCTCTTATCTCAATGAAATGGGTTGAAACAGGGAATTTCGGA
CTTGAACTCGAGAGTTCCAGGAGTAGAGCTCGCGAAACTCTCAGCCGAGACATGCCTCTG
CCCCATGCACTCGAGCACCACCACCACCACCACTGAGATCCGGCTGCTAACAAAGCCCGA

Protein sequence predicted based on nucleotide sequence

MPSLKDYTEESAQIPEMAEKLGIPTSRKETKTLELPVSIDFRNWCSPIEDQMDLGSCTAH
AGVGVVEYFERRAFGKHIDGSRFFVYKTTRNLMGVKGDTGAWLRNTMGALALCGVPPEKY
FPYTSRKTPGPDGEPTFDDEPSSFVYSLADNFEALNYFCHDPQGMNLPPEDVILSVKKYL
AAGIPSMFGFYGFPSFKDTDVKGGIPFPGPDERSIWGHAVVAVGYDDNMKIRNTLSNKET
TGAFLIRNSWGTAWGDKGYGWLPYEYALNKFALDFWSLISMKWVETGNFGLELESSRSRA
RETLSRDMPLPHA

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in MvR97A
Recommended expression in:
Not Applicable