Atu3204 (A. tumefaciens )
In pET21_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  Atu3204
Gene Name:  diguanylate cyclase
Original Clone ID:
AtR344E-21.4
Keyword:
targeted domain
Species:
Agrobacterium tumefaciens
Type:
cDNA
Vector Name:
pET21_NESG
Format: 
FUSION
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
Rutgers University
PSI:Biology
Northeast Structural Genomics Consortium
AtCD00589308
Price: 
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AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 498nts
Open reading frame: 51 to 548

AATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGCGGAAG
CCTATCTCGAATATCTCGGCAAGCACGATGTGCTGTCCAAGCTGCATAACCGCGCTTTCT
ATTCGGACGAGCTGAACCGGCTGGAGCGCAGCGCGCTCAGGCCGGTTTCTGCCGTCGTCA
TCGATCTCAACGGCCTGAAGGAGGCGAATGACAAGCTCGGCCATGATGCCGGCGACGCGT
TGCTGCGGCGGGTGGGCGAGGTGCTGAACGGCGTGGTGACTGCGCCAAATCATGCCGCGC
GCATCGGCGGCGACGAATTCGCCATCCTTCTTCCCGGCGCCGACAAGCAGGTGGCCGCCG
CCATGGTCGACACGGTCTATGAGTTGCTGAAGATCAATAACCAGTTCTATTCCAGCGCAC
CGCTCAGCCTGTCGCTGGGTGTGGCGACCACCGAAGCCGGTGAGACGATGGAATCACTAG
TGCGCCGGGCCGATATGAACATGTACGAGCAGAAGAATGCCTATTATGCGGCCCTGCGCA
ATCGATCGCTCGAGCACCACCACCACCACCACTGAGATCCGGCTGCTAACAAAGCCCGA

Protein sequence predicted based on nucleotide sequence

MAEAYLEYLGKHDVLSKLHNRAFYSDELNRLERSALRPVSAVVIDLNGLKEANDKLGHDA
GDALLRRVGEVLNGVVTAPNHAARIGGDEFAILLPGADKQVAAAMVDTVYELLKINNQFY
SSAPLSLSLGVATTEAGETMESLVRRADMNMYEQKNAYYAALRNRS

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
a46t,T16S;a124g,I42V;

g369a,A123A
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in AtR344E
Recommended expression in:
Not Applicable