DNASU Plasmid | ACTL6A (H. sapiens )<br>In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: ACTL6A

Gene Name: actin-like 6A

Original Clone ID:

HR2893A-55-429-14.33

Keyword:

targeted domain

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pET15_NESG

Format:

CLOSED

Source:

Northeast Structural Genomics Consortium

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

Rutgers University
PSI:Biology
Northeast Structural Genomics Consortium

HsCD00589647

Price:

AVAILABLE

No restriction
Special MTA: PSI/NESG

Insert Sequence
Protein Sequence

Insert sequence: 1131nts

Open reading frame: 26 to 1156

Reference Sequence Alignment

CCATCACCATCACCATCACAGCCATATGGAAATAGATGGCGATAAAGGCAAACAAGGCGG
TCCCACCTACTACATAGATACTAATGCTCTGCGTGTTCCGAGGGAGAATATGGAGGCCAT
TTCACCTCTAAAAAATGGGATGGTTGAAGACTGGGATAGTTTCCAAGCTATTTTGGATCA
TACCTACAAAATGCATGTCAAATCAGAAGCCAGTCTCCATCCTGTTCTCATGTCAGAGGC
ACCGCGGAATACTAGAGCAAAGAGAGAGAAACTGACAGAGTTAATGTTTGAACACTACAA
CATCCCTGCCTTCTTCCTTTGCAAAACTGCAGTTTTGACAGCATTTGCTAATGGTCGTTC
TACTGGGCTGATTTTGGACAGTGGAGCCACTCATACCACGGCAATTCCAGTCCACGATGG
CTATGTCCTTCAACAAGGCATTGTGAAATCCCCTCTTGCTGGAGACTTTATTACTATGCA
GTGCAGAGAACTCTTCCAAGAAATGAATATTGAATTGGTTCCTCCATATATGATTGCATC
AAAAGAAGCTGTTCGTGAAGGATCTCCAGCAAACTGGAAAAGAAAAGAGAGGTTGCCTCA
GGTTACGAGGTCTTGGCACAATTATATGTGTAATTGTGTTATCCAGGATTTTCAAGCTTC
GGTACTTCAAGTGTCAGATTCAACTTATGATGAACAAGTGGCTGCACAGATGCCAACTGT
TCATTATGAATTCCCCAATGGCTACGATTGTGATTTTGGTGCAGAGCGGCTAGAGATTCC
AGAAGGATTATTTGACCCTTCCAATGTAAAGGGGTTATCAGGAAACACAATGTTAGGAGT
CAGTCATGTTGTCACCACAAGTGTTGGGATGTGTGATATTGATGTCAGACCAGGTCTCTA
TGGCAGTGTAATAGTGGCAGGAGGAAACACACTAATACAGAGTTTTACTGACAGGTTGAA
TAGAGAGCTGTCTCAGAAAACTCCTCCAAGTATGCGGTTGAAATTGATTGCAAATAATAC
AACAGTGGAACGGAGGTTTAGCTCATGGATTGGCGGCTCCATTCTAGCCTCTTTGGGTAC
CTTTCAACAGATGTGGATTTCCAAGCAAGAATATGAAGAAGGAGGGAAGCAGTGTGTAGA
AAGAAAATGCCCTTAG

Protein sequence predicted based on nucleotide sequence

MEIDGDKGKQGGPTYYIDTNALRVPRENMEAISPLKNGMVEDWDSFQAILDHTYKMHVKS
EASLHPVLMSEAPRNTRAKREKLTELMFEHYNIPAFFLCKTAVLTAFANGRSTGLILDSG
ATHTTAIPVHDGYVLQQGIVKSPLAGDFITMQCRELFQEMNIELVPPYMIASKEAVREGS
PANWKRKERLPQVTRSWHNYMCNCVIQDFQASVLQVSDSTYDEQVAAQMPTVHYEFPNGY
DCDFGAERLEIPEGLFDPSNVKGLSGNTMLGVSHVVTTSVGMCDIDVRPGLYGSVIVAGG
NTLIQSFTDRLNRELSQKTPPSMRLKLIANNTTVERRFSSWIGGSILASLGTFQQMWISK
QEYEEGGKQCVERKCP*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

t220c,W74R;t375g,T125T;

a566g,K189R;a721g,N241D;

a748g,K250E;a859g,I287V

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinPurified: Not_Tested

ProteinExpressed: Protein_Confirmed

SolubleProtein: Tested_Not_Soluble

Find experimental protocols in HR2893A

Recommended expression in:

Not Applicable

Vector Name:

pET15_NESG

Synonyms:

pET15, pET15-modified, pET15Cc, pET15_NESG

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.

Comments:

Modified Novagen pET vector-common MCS

Size (bp):

5723

Parent Vector:

None

Properties:

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker

Author Name:

Gaetano T. Montleione
Jessica Bearden
Northeast Structural Genomics Consortium

Publications:

PMID: 15808222

Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:

Vector Sequence:

Sequence:

TTCTCATGTTTGACAGCTTATCATCGATAAGCTAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCGAGAAGCTTTGCGTCGACAGAGCTCGCAGGATCCGCAGAATTCGCCATATGGCTGTGATGGTGATGGTGATGGCCCATGGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAA

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI)

327

452

bacterial origin

ori

ColE1 origin of replication

3881

4563

gene fragment

ROP

repressor of primer ROP sequence

3281

3457

primer

T7 forward

T7 forward sequencing primer

481

496

primer

T7 reverse

T7 reverse sequencing primer

257

275

promoter

T7 promoter

478

497

repressor binding site

lacO

lacO regulatory sequence

456

478

selectable marker

AmpR

ampicillin resistance gene

4661

5230

tag

His

N-terminal 6x His tag

387

404

trxn termination sequence

T7 term

T7 termination sequence

257

275

Gene

Gene Symbol :

ACTL6A

Symbol Nomenclature :

ACTL6A

SYNONYM :

ACTL6; ARPN-BETA; Arp4; BAF53A; INO80K

Designation :

Full Nomenclature :

actin-like 6A

GENEID :

GI :

30089999

GenBank Accession :

NM_178042

HGNC :

24124

MIM :

604958

Vega :

OTTHUMG00000157782

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00589647      ------------------------------------------------------------
NM_004301.3       ATGAGCGGCGGCGTGTACGGGGGAGATGAAGTTGGAGCCCTTGTTTTTGACATTGGATCC
NM_178042.2       ------------------------------------------------------------
NM_177989.2       ------------------------------------------------------------
                                                                              

HsCD00589647      ------------------------------------------------------------
NM_004301.3       TATACTGTGAGAGCTGGTTATGCTGGTGAGGACTGCCCCAAGGTGGATTTTCCTACAGCT
NM_178042.2       ------------------------------------------------------------
NM_177989.2       ------------------------------------------------------------
                                                                              

HsCD00589647      --------------CCATCACCATCACCATCACAGCCATATGGAAATAGATGGCGATAAA
NM_004301.3       ATTGGTATGGTGGTAGAAAGAGATGACGGAAGCACATTAATGGAAATAGATGGCGATAAA
NM_178042.2       ------ATGGTGGTAGAAAGAGATGACGGAAGCACATTAATGGAAATAGATGGCGATAAA
NM_177989.2       ------ATGGTGGTAGAAAGAGATGACGGAAGCACATTAATGGAAATAGATGGCGATAAA
                                . *:... ** ** .:..** . ::*********************

HsCD00589647      GGCAAACAAGGCGGTCCCACCTACTACATAGATACTAATGCTCTGCGTGTTCCGAGGGAG
NM_004301.3       GGCAAACAAGGCGGTCCCACCTACTACATAGATACTAATGCTCTGCGTGTTCCGAGGGAG
NM_178042.2       GGCAAACAAGGCGGTCCCACCTACTACATAGATACTAATGCTCTGCGTGTTCCGAGGGAG
NM_177989.2       GGCAAACAAGGCGGTCCCACCTACTACATAGATACTAATGCTCTGCGTGTTCCGAGGGAG
                  ************************************************************

HsCD00589647      AATATGGAGGCCATTTCACCTCTAAAAAATGGGATGGTTGAAGACTGGGATAGTTTCCAA
NM_004301.3       AATATGGAGGCCATTTCACCTCTAAAAAATGGGATGGTTGAAGACTGGGATAGTTTCCAA
NM_178042.2       AATATGGAGGCCATTTCACCTCTAAAAAATGGGATGGTTGAAGACTGGGATAGTTTCCAA
NM_177989.2       AATATGGAGGCCATTTCACCTCTAAAAAATGGGATGGTTGAAGACTGGGATAGTTTCCAA
                  ************************************************************

HsCD00589647      GCTATTTTGGATCATACCTACAAAATGCATGTCAAATCAGAAGCCAGTCTCCATCCTGTT
NM_004301.3       GCTATTTTGGATCATACCTACAAAATGCATGTCAAATCAGAAGCCAGTCTCCATCCTGTT
NM_178042.2       GCTATTTTGGATCATACCTACAAAATGCATGTCAAATCAGAAGCCAGTCTCCATCCTGTT
NM_177989.2       GCTATTTTGGATCATACCTACAAAATGCATGTCAAATCAGAAGCCAGTCTCCATCCTGTT
                  ************************************************************

HsCD00589647      CTCATGTCAGAGGCACCGCGGAATACTAGAGCAAAGAGAGAGAAACTGACAGAGTTAATG
NM_004301.3       CTCATGTCAGAGGCACCGTGGAATACTAGAGCAAAGAGAGAGAAACTGACAGAGTTAATG
NM_178042.2       CTCATGTCAGAGGCACCGTGGAATACTAGAGCAAAGAGAGAGAAACTGACAGAGTTAATG
NM_177989.2       CTCATGTCAGAGGCACCGTGGAATACTAGAGCAAAGAGAGAGAAACTGACAGAGTTAATG
                  ****************** *****************************************

HsCD00589647      TTTGAACACTACAACATCCCTGCCTTCTTCCTTTGCAAAACTGCAGTTTTGACAGCATTT
NM_004301.3       TTTGAACACTACAACATCCCTGCCTTCTTCCTTTGCAAAACTGCAGTTTTGACAGCATTT
NM_178042.2       TTTGAACACTACAACATCCCTGCCTTCTTCCTTTGCAAAACTGCAGTTTTGACAGCATTT
NM_177989.2       TTTGAACACTACAACATCCCTGCCTTCTTCCTTTGCAAAACTGCAGTTTTGACAGCATTT
                  ************************************************************

HsCD00589647      GCTAATGGTCGTTCTACTGGGCTGATTTTGGACAGTGGAGCCACTCATACCACGGCAATT
NM_004301.3       GCTAATGGTCGTTCTACTGGGCTGATTTTGGACAGTGGAGCCACTCATACCACTGCAATT
NM_178042.2       GCTAATGGTCGTTCTACTGGGCTGATTTTGGACAGTGGAGCCACTCATACCACTGCAATT
NM_177989.2       GCTAATGGTCGTTCTACTGGGCTGATTTTGGACAGTGGAGCCACTCATACCACTGCAATT
                  ***************************************************** ******

HsCD00589647      CCAGTCCACGATGGCTATGTCCTTCAACAAGGCATTGTGAAATCCCCTCTTGCTGGAGAC
NM_004301.3       CCAGTCCACGATGGCTATGTCCTTCAACAAGGCATTGTGAAATCCCCTCTTGCTGGAGAC
NM_178042.2       CCAGTCCACGATGGCTATGTCCTTCAACAAGGCATTGTGAAATCCCCTCTTGCTGGAGAC
NM_177989.2       CCAGTCCACGATGGCTATGTCCTTCAACAAGGCATTGTGAAATCCCCTCTTGCTGGAGAC
                  ************************************************************

HsCD00589647      TTTATTACTATGCAGTGCAGAGAACTCTTCCAAGAAATGAATATTGAATTGGTTCCTCCA
NM_004301.3       TTTATTACTATGCAGTGCAGAGAACTCTTCCAAGAAATGAATATTGAATTGGTTCCTCCA
NM_178042.2       TTTATTACTATGCAGTGCAGAGAACTCTTCCAAGAAATGAATATTGAATTGGTTCCTCCA
NM_177989.2       TTTATTACTATGCAGTGCAGAGAACTCTTCCAAGAAATGAATATTGAATTGGTTCCTCCA
                  ************************************************************

HsCD00589647      TATATGATTGCATCAAAAGAAGCTGTTCGTGAAGGATCTCCAGCAAACTGGAAAAGAAAA
NM_004301.3       TATATGATTGCATCAAAAGAAGCTGTTCGTGAAGGATCTCCAGCAAACTGGAAAAGAAAA
NM_178042.2       TATATGATTGCATCAAAAGAAGCTGTTCGTGAAGGATCTCCAGCAAACTGGAAAAGAAAA
NM_177989.2       TATATGATTGCATCAAAAGAAGCTGTTCGTGAAGGATCTCCAGCAAACTGGAAAAGAAAA
                  ************************************************************

HsCD00589647      GAGAGGTTGCCTCAGGTTACGAGGTCTTGGCACAATTATATGTGTAATTGTGTTATCCAG
NM_004301.3       GAGAAGTTGCCTCAGGTTACGAGGTCTTGGCACAATTATATGTGTAATTGTGTTATCCAG
NM_178042.2       GAGAAGTTGCCTCAGGTTACGAGGTCTTGGCACAATTATATGTGTAATTGTGTTATCCAG
NM_177989.2       GAGAAGTTGCCTCAGGTTACGAGGTCTTGGCACAATTATATGTGTAATTGTGTTATCCAG
                  ****.*******************************************************

HsCD00589647      GATTTTCAAGCTTCGGTACTTCAAGTGTCAGATTCAACTTATGATGAACAAGTGGCTGCA
NM_004301.3       GATTTTCAAGCTTCGGTACTTCAAGTGTCAGATTCAACTTATGATGAACAAGTGGCTGCA
NM_178042.2       GATTTTCAAGCTTCGGTACTTCAAGTGTCAGATTCAACTTATGATGAACAAGTGGCTGCA
NM_177989.2       GATTTTCAAGCTTCGGTACTTCAAGTGTCAGATTCAACTTATGATGAACAAGTGGCTGCA
                  ************************************************************

HsCD00589647      CAGATGCCAACTGTTCATTATGAATTCCCCAATGGCTACGATTGTGATTTTGGTGCAGAG
NM_004301.3       CAGATGCCAACTGTTCATTATGAATTCCCCAATGGCTACAATTGTGATTTTGGTGCAGAG
NM_178042.2       CAGATGCCAACTGTTCATTATGAATTCCCCAATGGCTACAATTGTGATTTTGGTGCAGAG
NM_177989.2       CAGATGCCAACTGTTCATTATGAATTCCCCAATGGCTACAATTGTGATTTTGGTGCAGAG
                  ***************************************.********************

HsCD00589647      CGGCTAGAGATTCCAGAAGGATTATTTGACCCTTCCAATGTAAAGGGGTTATCAGGAAAC
NM_004301.3       CGGCTAAAGATTCCAGAAGGATTATTTGACCCTTCCAATGTAAAGGGGTTATCAGGAAAC
NM_178042.2       CGGCTAAAGATTCCAGAAGGATTATTTGACCCTTCCAATGTAAAGGGGTTATCAGGAAAC
NM_177989.2       CGGCTAAAGATTCCAGAAGGATTATTTGACCCTTCCAATGTAAAGGGGTTATCAGGAAAC
                  ******.*****************************************************

HsCD00589647      ACAATGTTAGGAGTCAGTCATGTTGTCACCACAAGTGTTGGGATGTGTGATATTGATGTC
NM_004301.3       ACAATGTTAGGAGTCAGTCATGTTGTCACCACAAGTGTTGGGATGTGTGATATTGACATC
NM_178042.2       ACAATGTTAGGAGTCAGTCATGTTGTCACCACAAGTGTTGGGATGTGTGATATTGACATC
NM_177989.2       ACAATGTTAGGAGTCAGTCATGTTGTCACCACAAGTGTTGGGATGTGTGATATTGACATC
                  ******************************************************** .**

HsCD00589647      AGACCAGGTCTCTATGGCAGTGTAATAGTGGCAGGAGGAAACACACTAATACAGAGTTTT
NM_004301.3       AGACCAGGTCTCTATGGCAGTGTAATAGTGGCAGGAGGAAACACACTAATACAGAGTTTT
NM_178042.2       AGACCAGGTCTCTATGGCAGTGTAATAGTGGCAGGAGGAAACACACTAATACAGAGTTTT
NM_177989.2       AGACCAGGTCTCTATGGCAGTGTAATAGTGGCAGGAGGAAACACACTAATACAGAGTTTT
                  ************************************************************

HsCD00589647      ACTGACAGGTTGAATAGAGAGCTGTCTCAGAAAACTCCTCCAAGTATGCGGTTGAAATTG
NM_004301.3       ACTGACAGGTTGAATAGAGAGCTGTCTCAGAAAACTCCTCCAAGTATGCGGTTGAAATTG
NM_178042.2       ACTGACAGGTTGAATAGAGAGCTGTCTCAGAAAACTCCTCCAAGTATGCGGTTGAAATTG
NM_177989.2       ACTGACAGGTTGAATAGAGAGCTGTCTCAGAAAACTCCTCCAAGTATGCGGTTGAAATTG
                  ************************************************************

HsCD00589647      ATTGCAAATAATACAACAGTGGAACGGAGGTTTAGCTCATGGATTGGCGGCTCCATTCTA
NM_004301.3       ATTGCAAATAATACAACAGTGGAACGGAGGTTTAGCTCATGGATTGGCGGCTCCATTCTA
NM_178042.2       ATTGCAAATAATACAACAGTGGAACGGAGGTTTAGCTCATGGATTGGCGGCTCCATTCTA
NM_177989.2       ATTGCAAATAATACAACAGTGGAACGGAGGTTTAGCTCATGGATTGGCGGCTCCATTCTA
                  ************************************************************

HsCD00589647      GCCTCTTTGGGTACCTTTCAACAGATGTGGATTTCCAAGCAAGAATATGAAGAAGGAGGG
NM_004301.3       GCCTCTTTGGGTACCTTTCAACAGATGTGGATTTCCAAGCAAGAATATGAAGAAGGAGGG
NM_178042.2       GCCTCTTTGGGTACCTTTCAACAGATGTGGATTTCCAAGCAAGAATATGAAGAAGGAGGG
NM_177989.2       GCCTCTTTGGGTACCTTTCAACAGATGTGGATTTCCAAGCAAGAATATGAAGAAGGAGGG
                  ************************************************************

HsCD00589647      AAGCAGTGTGTAGAAAGAAAATGCCCTTAG
NM_004301.3       AAGCAGTGTGTAGAAAGAAAATGCCCTTGA
NM_178042.2       AAGCAGTGTGTAGAAAGAAAATGCCCTTGA
NM_177989.2       AAGCAGTGTGTAGAAAGAAAATGCCCTTGA
                  ****************************..

Pathways

NCI :

C-MYC pathway

NCI :

Validated targets of C-MYC transcriptional activation

Reactome :

Chromatin modifying enzymes

Reactome :

Chromatin organization

Reactome :

HATs acetylate histones

Protein

SMART domain :

ACTIN : Actin

Structural Annotation Wiki :

HR2893A

UniProt :

O96019

HPRD :

05389

Clone

Cloning Information :

630579

Original Clone ID :

HR2893A-55-429-14.33

Experimental

TargetTrack - Experimental data :

HR2893A

Organism

Species Specific ID:

Genome

Chromosome :

Map Location :

3q26.33

Ensembl :

ENSG00000136518

Reagents

Labome :

ACTL6A-antibody

ACTL6A (H. sapiens )In pET15_NESG (His-tagged bacterial expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Pathways

Protein

Clone

Experimental

Organism

Genome

Reagents

ACTL6A (H. sapiens )
In pET15_NESG (His-tagged bacterial expression vector)