DNASU Plasmid | Caci_1943 (C. acidiphila DSM 44928 )<br>In pMCSG68 (Bacterial Expression)

Explanation of Terms

Gene:

Gene Symbol: Caci_1943

Gene Name: LacI family transcriptional regulator

Original Clone ID:

APC110309.102

Keyword:

None

Species:

Catenulispora acidiphila DSM 44928

Type:

cDNA

Vector Name:

pMCSG68

Format:

CLOSED

Source:

Midwest Center for Structural Genomics

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

Argonne National Laboratory
Shonda Clancy
PSI:Biology
Midwest Center for Structural Genomics

CaCD00589719

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 966nts

Open reading frame: 51 to 1016

ATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCCGCCCCGACG
CGGTGTCCCCGGGCACCCGGGCACGGGTCCAGGCGGCGATCGCGGAGCTGGGCTTCGTGC
GCAGCGAGAGCGCGCGGCTGCTGCGCGCCGGCCGCAGCCAGGTGCTGGCCCTGCTAGCGC
TGGACCTGGCCGACCCGTTCTTCGTCGGCGTGCTGCGCGGCGCCGAGCAGGCCGCCCGCG
CCGCCGATCTCGGCGTGCTGGTGATGGACAGCGCCTCCAGCGCCGCCACCGAGGCCACGC
ACCTGGTGATGCTGGCCCAGCAACGGGTTCGGGGCGTGTTGGTGACTCCGGCGGACGCCA
GCGGCGCGACGCTGGCGGAGTTCGGCCGCGCGCGGATTCCGTACGTGTTGGTGGATCGTG
ACATACCGGGCGGAAACGGATGTCGGGTGCTCGTGGACGACGTCGCCGGCGGCGCGATGG
CGGTCGCGCACCTGATCGCCGGCGGACACCGGCGCATCGCCTACGTCGCCGGACCGCTGG
AGCTGCCGCAGTACCGGGACCGGCTGGAAGGCGCGCGTCAGGCGATCGCCGAAGCCGATC
CGGACGTGGAGCTGGTGACGCTGCTCGGCGCGCGCGGGGACGTGGCGGCCGGACGGGACG
CCGCGGCGCGCATGCTCGGGCTGGGGGAGCGCCCGACCGGGGTGTTCTGCGGCAACGACC
TGATCGCCTTGGGGATGCTGCAAGGGCTGTTCGCCGCCAGTGTGGCGGTACCGCAGGACG
TGGCGATCGTTGGCTACGACGACCTGGAGGTCGCGGCGGCGGCGGTCGTGCCACTGTCGT
CGATACGGCGCCCCGCCTCGCGCGTGGGCAAGGAGGCCGCGGAGCTTCTGGTCGAGGAGA
GCGGGGCGGCGGCGGGGCAGTATGTGCACCGCGAGCTGGTTTTTCAGCCGGAGCTGGTGG
TGCGGGCTTCTAGCTTGGGGGCGGCGGCTTCGACGGCTCGAAACCCGGACAGCTAACATT
GGAAGTGGATAATAA

Protein sequence predicted based on nucleotide sequence

RPDAVSPGTRARVQAAIAELGFVRSESARLLRAGRSQVLALLALDLADPFFVGVLRGAEQ
AARAADLGVLVMDSASSAATEATHLVMLAQQRVRGVLVTPADASGATLAEFGRARIPYVL
VDRDIPGGNGCRVLVDDVAGGAMAVAHLIAGGHRRIAYVAGPLELPQYRDRLEGARQAIA
EADPDVELVTLLGARGDVAAGRDAAARMLGLGERPTGVFCGNDLIALGMLQGLFAASVAV
PQDVAIVGYDDLEVAAAAVVPLSSIRRPASRVGKEAAELLVEESGAAAGQYVHRELVFQP
ELVVRASSLGAAASTARNPDS*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

c871t,H291Y

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinPurified: Not_Tested

ProteinExpressed: Protein_Confirmed

SolubleProtein: Tested_Not_Soluble

Find experimental protocols in MCSG-APC110309

Recommended expression in:

Not Applicable

Vector Name:

pMCSG68

Synonyms:

Sequencing Primer:

Forward: T7short

Reverse: T7 terminator

Description:

Bacterial expression vector with a T7 promoter and TEV-cleavable N-terminal 6xHis and StrepII tags; rare argU and ileX codons; ampicillin resistance in bacteria; ligation independent cloning

Comments:

BglI-XbaI fragment removed from pMCSG57 and ligated into pMCSG53 digested with the same enzymes

Size (bp):

4832

Parent Vector:

None

Properties:

bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker

Author Name:

Midwest Center for Structural Genomics
PSI:Biology
Midwest Center for Structural Genomics

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

ATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCAGTGGTGGTGGTGGTGGTGCTCGAGTGCGGCCGCAAGCTTGTCGACGGAGCTCGAATTCGGATCCGTTATCCACTTCCAATATTGGATTGGAAGTACAGGTTCTCGGTACCCTTTTCGAACTGCGGATGAGACCACAGATCTACACCAGAAGAATGATGATGATGATGGTGCATATGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGTGGATTCAGTCAGGCGTCCCATTATCAGTGCTTCAGGAAATGGGCGGATGGGAGTCCATAGAAATGGTTCGTAGGTATGCTCACCTTGCGCCTAATCATTTGACAGAGCATGCGAGGAAAATAGACGACATTTTTGGTGATAATGTCCCAAATATGTCCCACTCTGGAATTATGGAGGATATAAAGAAGGCGTAACTGATTGAATTGTAATGGCGCGCCCTGCAGGATTCGAACCTGCGGCCCACGACTTAGAAGGTCGTTGCTCTATCCAACTGAGCTAAGGGCGCGTTGATACCGCAATGCGGTGTAATCGCGTGAATTATACGGTCAACCCTTGCTGAGTCAATGGCTTTTGATCTCGCCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCAAAAAGGCTGACGATTTCTCGTCAGCCTTTGCTATATCTGGTGGCCCCTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAGGGGCCATGGTAGCGGATTATAAAGTAACTCCGTGTCGCAATCCGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTACAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAAATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTAAATTGTAAGCGTTAATGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCCCATTCGCCA

Vector Features:

Type

Name

Description

Start

End

bacterial origin

pBR322 origin

pBR322 origin of replication

2825

3413

ligation independent cloning

LIC

ligation independent cloning site

204

235

promoter

T7 promoter

384

400

protease cleavage site

TEV

TEV protease cleavage site

223

243

repressor protein gene

LacI

Lac I gene

1437

2396

selectable marker

AmpR

ampicillin resistance gene

3584

4444

tRNA

ileX

ileX tRNA gene

983

1134

tRNA

argU

argU tRNA gene

467

853

tag

strep

N-terminal strepII tag

250

273

tag

His

N-terminal 6xHis tag

292

309

trxn regulatory element

lac operator

lac operator sequence

359

381

trxn termination sequence

T7 term

T7 terminator

Gene

Gene Symbol :

Caci_1943

GENEID :

8333286

Locus Tag :

Caci_1943

GI :

256391140

Target GenBank:

None

Protein

SMART domain :

HTH_LACI : helix_turn _helix lactose operon repressor

Structural Annotation Wiki :

MCSG-APC110309

Clone

Cloning Information :

631768

Original Clone ID :

APC110309.102

Experimental

TargetTrack - Experimental data :

MCSG-APC110309

Organism

Species Specific ID:

8333286

Caci_1943 (C. acidiphila DSM 44928 )In pMCSG68 (Bacterial Expression)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Protein

Clone

Experimental

Organism

Caci_1943 (C. acidiphila DSM 44928 )
In pMCSG68 (Bacterial Expression)