fas (M. tuberculosis H37Rv ) in pMCSG68 (Bacterial Expression)

Explanation of Terms

Gene:
Gene Symbol:  fas
Gene Name:  Probable fatty acid synthase Fas (fatty acid synthetase)
Original Clone ID:
APC105953.123
Keyword:
None
Species:
Mycobacterium tuberculosis H37Rv
Type:
cDNA
Vector Name:
pMCSG68
Format: 
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Argonne National Laboratory
PSI:Biology
Midwest Center for Structural Genomics
MtCD00590006
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1311nts
Open reading frame: 51 to 1361

ATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCTCGGAGGCCG
ATGCGCGCGCCTTCGTCGAGTTCGATCCCGAGCACACGGTCATCCGGCCGGTGCCCGACT
CCACCGACTGGCAGGTCATCCGCAAGGCCGGCACCGAGATCCGGGTGCCGCGAAAGACCA
AGCTGTCCCGCGTCGTCGGCGGCCAGATCCCGACCGGGTTCGACCCGACGGTGTGGGGCA
TCAGCGCAGACATGGCCGGTTCCATCGACCGGTTGGCGGTATGGAACATGGTGGCGACCG
TCGACGCGTTCCTGTCGTCCGGTTTCAGCCCGGCCGAGGTGATGCGTTACGTGCACCCGA
GTTTGGTGGCCAACACCCAGGGCACCGGCATGGGCGGCGGCACGTCGATGCAGACGATGT
ACCACGGCAATCTGTTGGGCCGCAACAAGCCGAACGACATCTTCCAGGAAGTCTTGCCGA
ATATCATTGCCGCGCACGTGGTTCAGTCCTACGTCGGTAGCTACGGTGCGATGATCCACC
CGGTAGCCGCGTGCGCCACCGCCGCGGTGTCGGTCGAGGAAGGTGTCGACAAGATCCGGT
TGGGCAAGGCTCAACTGGTGGTGGCCGGCGGCCTGGATGACCTGACGCTGGAGGGCATCA
TCGGATTCGGTGACATGGCCGCCACCGCCGACACGTCCATGATGTGCGGCCGCGGCATCC
ACGACTCGAAGTTTTCCCGGCCCAACGACCGCCGCCGTCTGGGCTTCGTCGAAGCCCAAG
GCGGCGGGACGATCCTGTTGGCCCGCGGGGACCTGGCGCTGCGGATGGGGCTGCCGGTGC
TGGCGGTGGTGGCGTTCGCGCAGTCGTTCGGCGACGGCGTGCACACCTCGATCCCGGCCC
CGGGCCTGGGCGCGCTGGGGGCGGGCCGCGGCGGCAAGGATTCACCGCTGGCGCGGGCGC
TGGCCAAGCTGGGCGTGGCCGCCGACGACGTGGCGGTCATCTCCAAGCACGACACCTCGA
CGCTGGCCAACGATCCCAACGAGACCGAGTTGCATGAACGGCTCGCCGACGCCCTGGGCC
GTTCCGAGGGCGCCCCGCTGTTCGTGGTGTCGCAGAAGAGCCTGACCGGCCACGCCAAGG
GCGGCGCGGCGGTCTTCCAGATGATGGGGCTCTGCCAGATATTGCGGGATGGGGTGATCC
CACCCAACCGCAGCCTCGACTGCGTCGACGACGAGCTGGCCGGCTCCGCGCATTTCGTGT
GGGTGCGTGACACGTTGCGGCTCGGCGGCAAGTTCCCACTCAAGGCCGGCATGCTGACCA
GCCTCGGGTTCGGCCATGTGTCGGGCCTGGTCGCGTTGTAACATTGGAAGTGGATAATAA

Protein sequence predicted based on nucleotide sequence

SEADARAFVEFDPEHTVIRPVPDSTDWQVIRKAGTEIRVPRKTKLSRVVGGQIPTGFDPT
VWGISADMAGSIDRLAVWNMVATVDAFLSSGFSPAEVMRYVHPSLVANTQGTGMGGGTSM
QTMYHGNLLGRNKPNDIFQEVLPNIIAAHVVQSYVGSYGAMIHPVAACATAAVSVEEGVD
KIRLGKAQLVVAGGLDDLTLEGIIGFGDMAATADTSMMCGRGIHDSKFSRPNDRRRLGFV
EAQGGGTILLARGDLALRMGLPVLAVVAFAQSFGDGVHTSIPAPGLGALGAGRGGKDSPL
ARALAKLGVAADDVAVISKHDTSTLANDPNETELHERLADALGRSEGAPLFVVSQKSLTG
HAKGGAAVFQMMGLCQILRDGVIPPNRSLDCVDDELAGSAHFVWVRDTLRLGGKFPLKAG
MLTSLGFGHVSGLVAL*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in MCSG-APC105953
Recommended expression in:
Not Applicable