ARAF (H. sapiens )
In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  ARAF
Gene Name:  v-raf murine sarcoma 3611 viral oncogene homolog
Original Clone ID:
HR6357-84-606-15.8
Keyword:
targeted domain
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pET15_NESG
Format: 
CLOSED
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
Rutgers University
PSI:Biology
Northeast Structural Genomics Consortium
HsCD00590529
Price: 
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AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 1575nts
Open reading frame: 14 to 1588

CCATCACAGCCATATGGAGGAGCTCATTGTCGAGGTCCTTGAAGATGTCCCGCTGACCAT
GCACAATTTTGTACGGAGGACCTTCTTCAGCCTGGCGTTCTGTGACTTCTGCCTTAAGTT
TCTGTTCCATGGCTTCCGTTGCCAAACCTGTGGCTACAAGTTCCACCAGCATTGTTCCTC
CAAGGTCCCCACAGTCTGTGTTGACATGAGTACCAACCGCCAACAGTTCTACCACAGTGT
CCAGGATTTGTCCGGAGGCTCCAGACAGCATGAGGCTCCCTCGAACCGCCCCCTGAATGA
GTTGCTAACCCCCCAGGGTCCCAGCCCCCGCACCCAGCACTGTGACCCGGAGCACTTCCC
CTTCCCTGCCCCAGCCAATGCCCCCCTACAGCGCATCCGCTCCACGTCCACTCCCAACGT
CCATATGGTCAGCACCACGGCCCCCATGGACTCCAACCTCATCCAGCTCACTGGCCAGAG
TTTCAGCACTGATGCTGCCGGTAGTAGAGGAGGTAGTGATGGAACCCCCCGGGGGAGCCC
CAGCCCAGCCAGCGTGTCCTCGGGGAGGAAGTCCCTACATTCCAAGTCACCAGCAGAGCA
GCGCGAGCGGAAGTCCTTGGCCGATGACAAGAAGAAAGTGAAGAACCTGGGGTACCGGGA
CTCAGGCTATTACTGGGAGGTACCACCCAGTGAGGTGCAGCTGCTGAAGAGGATCGGGAC
GGGCTCGTTTGGCACCGTGTTTCGAGGGCGGTGGCATGGCGATGTGGCCGTGAAGGTGCT
CAAGGTGTCCCAGCCCACAGCTGAGCAGGCCCAGGCTTTCAAGAATGAGATGCAGGTGCT
CAGGAAGACGCGACATGTCACCATCTTGCTGTTTATGGGCTTCATGACCCGGCCGGGATT
TGCCATCATCACACAGTGGTGTGAGGGCTCCGGCCTCTACCATCACCTGCATGTGGCCGA
CACACGCTTCGACATGGTCCAGCTCATCGACGTGGCCCGGCAGACTGCCCAGGGCATGGA
CTACCTCCATGCCAAGAACATCATCCACCGAGATCTCAAGTCTAACAACATCTTCCTACA
TGAGGGGCTCACGGTGAAGATCGGTGACTTTGGCTTGGCCACAGTGAAGACTCGATGGAG
CGGGGCCCAGCCCTTGGAGCAGCCCTCAGGATTTGTGCTGTGGATGGCAGCTGAGGTGAT
CCGTATGCAGGACCCGAACCCCTACAGCTTCCAGTCAGACGTCTATGCCTACGGGGTTGT
GCTCTACGAGCTTATGACTGGCTCACTGCCTTACAGCCACATTGGCTGCCGTGACCAGAT
TATCTTTATGGTGGGCCGTGGCTATCTGTCCCCGGACCTCAGCAAAATCTCCAGCAACTG
CCCCAAGGCCATGCGGCGCCTGCTGTCTGACTGCCTCAAGTTCCAGCGGGAGGAGCGGCC
CCTCTTCCCCCAGATCCTGGCCACAATTGAGCTGCTGCAACGGTCACTCCCCAAGATTGA
GCGGAGTGCCTCGGAACCCTCCTTGCACCGCACCCAGGCCGATGAGTTGCCTGCCTGCCT
ACTCAGCGCAGCCCGCCTTGTGCCTTAG

Protein sequence predicted based on nucleotide sequence

MEELIVEVLEDVPLTMHNFVRRTFFSLAFCDFCLKFLFHGFRCQTCGYKFHQHCSSKVPT
VCVDMSTNRQQFYHSVQDLSGGSRQHEAPSNRPLNELLTPQGPSPRTQHCDPEHFPFPAP
ANAPLQRIRSTSTPNVHMVSTTAPMDSNLIQLTGQSFSTDAAGSRGGSDGTPRGSPSPAS
VSSGRKSLHSKSPAEQRERKSLADDKKKVKNLGYRDSGYYWEVPPSEVQLLKRIGTGSFG
TVFRGRWHGDVAVKVLKVSQPTAEQAQAFKNEMQVLRKTRHVTILLFMGFMTRPGFAIIT
QWCEGSGLYHHLHVADTRFDMVQLIDVARQTAQGMDYLHAKNIIHRDLKSNNIFLHEGLT
VKIGDFGLATVKTRWSGAQPLEQPSGFVLWMAAEVIRMQDPNPYSFQSDVYAYGVVLYEL
MTGSLPYSHIGCRDQIIFMVGRGYLSPDLSKISSNCPKAMRRLLSDCLKFQREERPLFPQ
ILATIELLQRSLPKIERSASEPSLHRTQADELPACLLSAARLVP*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
a65g,K22R;c563t,P188L;

a848c,N283T;a919g,S307G;

c1160t,S387F
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
SolubleProtein: Not_Tested
ProteinExpressed: Tested_Not_Found
Find experimental protocols in HR6357
Recommended expression in:
Not Applicable