Sked_07700 (S. keddieii DSM 10542 )
In pMCSG73 (Bacterial Expression)

Explanation of Terms

Gene:
Gene Symbol:  Sked_07700
Gene Name:  transcriptional regulator
Original Clone ID:
APC111027.102
Keyword:
None
Species:
Sanguibacter keddieii DSM 10542
Type:
cDNA
Vector Name:
pMCSG73
Format: 
FUSION
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
Argonne National Laboratory
Michael Endres
PSI:Biology
Midwest Center for Structural Genomics
SkCD00590717
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 996nts
Open reading frame: 4 to 999

GCCTCCTCCGGGCCGATCGCCGAGAAGACCCGCGCGAAGGTGCTCGCCGCCGCCGAGGAG
CTCGGCTACCGCGGCCCCAACCCCCTCGGGCGCCAGCTGCGCTCCGGGCGCTCCGGGATC
GTGGGCGTGGTCATCGGCGACAAGCTCCGCAGGTCCTTCCGCGACCCGGTCTCCGTGCAG
GTGCTCGACGGCCTCGCGAGCACCCTGGGTTCCATGGGGCTCGGGGTGCTGCTCATCCCC
GGTGCGAGCGACGAGGACGGGCTGCAGACGGTCGACCCGCTCATCGAGACCGCCGCGATG
GACGTCGCGATCCTCATCTGGGGCGTGCACGCGAACGACACGAGCGTCGAGGCGCTCCAG
CGTCGCGGTGTCCCCGTGGTCGTCGGCGAGGGCCGTCCGATCGAGGGAGCCCCGCTCGTC
GGCATCGACGACCGCGGTGGCTCGGCAGAACTCGCCCGCTACCTCACAGGACTCGGGCAC
GAGCGCATCGCGCTCATCACCCTCCCCTTCTCTGCCGACCGTCGCGCAGGACAGGCGGTC
GACCTCGACGACATCGAGTGGACGCCCACCCGCAACCGGCTGGCCGGCCTGCGCGACGCG
GGCGTCGAGCCCGTGGTCGTGTGGGAGACCGAGGCGTCGCTCGTCGAGCACGGGCACGCC
GCGGCCATGGAGCTCCTCTCCCCCGACCGCTGGCCCGACCCGGCGCTGCGCCCGACGGCG
ATCGTCGCCCAGTCCGACCTGCTCGCCGCCGGCGCGATGATCGGTGCGCGCGAACTCGGC
CTGCGCGTCCCCGAGGACGTGTCGATCGCCGGCTTCGACGGCCTCGACCTGCCCTGGCTC
GGGTCCGACGTCCTCACCACGGTCACCCAGCCGCTCGCCGCCAAGGGCGCAGCGCTCGGT
CACGCCGTCGAGGGCACCCTCGCGGGCGAGGACCCGGTGACGACGATCCTCGAGGTCGAG
CTCACCGTCGGCACGACGACAGGCCCCCCGGCGCAGCCC

Protein sequence predicted based on nucleotide sequence

SSGPIAEKTRAKVLAAAEELGYRGPNPLGRQLRSGRSGIVGVVIGDKLRRSFRDPVSVQV
LDGLASTLGSMGLGVLLIPGASDEDGLQTVDPLIETAAMDVAILIWGVHANDTSVEALQR
RGVPVVVGEGRPIEGAPLVGIDDRGGSAELARYLTGLGHERIALITLPFSADRRAGQAVD
LDDIEWTPTRNRLAGLRDAGVEPVVVWETEASLVEHGHAAAMELLSPDRWPDPALRPTAI
VAQSDLLAAGAMIGARELGLRVPEDVSIAGFDGLDLPWLGSDVLTTVTQPLAAKGAALGH
AVEGTLAGEDPVTTILEVELTVGTTTGPPAQP

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
g771a,E257E;t914c,I305T
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in MCSG-APC111027
Recommended expression in:
Not Applicable