Dtox_3626 (D. acetoxidans DSM 771 )
In pMCSG68 (Bacterial Expression)

Explanation of Terms

Gene:
Gene Symbol:  Dtox_3626
Gene Name:  N-acetylmuramoyl-L-alanine amidase
Original Clone ID:
APC100417.105
Keyword:
None
Species:
Desulfofarcimen acetoxidans DSM 771
Type:
cDNA
Vector Name:
pMCSG68
Format: 
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Argonne National Laboratory
Michael Endres
PSI:Biology
Midwest Center for Structural Genomics
DaCD00590861
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1338nts
Open reading frame: 51 to 1388

ATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCGCTTCCGTAG
CTTCAGTCAACGGTGACAATATTAATGTCAGGGAGGGACCTGGTACAACCTCCGATATTG
TTGGAGAGTTAAATAAGGGTGATAGTGTTACTGTTCTGGAAAAATCCGGTGATTGGTACA
AGGTTAAGCTGTCTAACGGTGACGGTTGGGTTTTAAGCAGTTTTTTAAATCTTAGTGAGC
AGAATTCTGACGATTCGGCAGACTGGCTCTACGCTGGGGCCGGTAGTAACGATAATCAGG
TTAAACCTGCTCAAACAGCTGCAACAGCACAAAAAGAGGTGACTCTGCCTGAATGGCTCC
GGCCAAGAGAGGGTAAGCTTGCATCTGCTGGTAACTCCGGCAGCACCGATAACTCCAATA
ACACGGGCAGCACCGGCAACTCGAACAACTCCAGTGATACAAACAACTCAGGCAGCAAAA
CCGGGGAACAGCCTTTTGTCTGGGACGGGGGGGAACTTACTAAGATAGATGTGTGTGACA
ATAGTGATGAAGTAGTTGTTACTGTCTATGCCACAGGTAAAATGCAGTTAAGCGCCTTTA
CTATTGACAACCCGTCTAGATTAGTTTTGGATTTAACTGGAGTAATACCGGGGGACATAC
CTTCCACTATGCAAGTATCTTCAAATATTGTTCAGCAAGTTCGCACTGCTTTATATAGCA
AGGAACCGGTTAAGTCCAGAGTTGTGCTTGATTTAATGAAACAGTCAGGATATAAAACTA
CTTTGTCAAGTGACCAAAAAACCTTAACTGTTCAGTTAGATAAAGGACAGCGGACTGTGG
TTAAAAGCGGCAAGAAGCTAATTGCGATAGATCCCGGACATGGTGGTAAGGATTGTGGGG
CTATCGGTTGTACCGGCTTATATGAAAAAGATGTAACCTTGGACGTTTCACGTCAGGTTG
TTGACTTGCTGAAGAACTCCGGCTATGACGCTGTGCTGACCAGAACAGATGACACCTATG
TAGGACTTGACGAGAGGACTGACTATGCTAACAGCCTGAATGCTGATTTATTTGTCAGTG
TGCATATAAACTCCAGTGAAGCACAGACTCCTTCAGGCACCAGTACTCACTATCGTTCTG
AGGAAGGAAAAGTCTTGAGCACATATATTCAGTCCGCTCTGATAGCAGGTTTGGGGCGCA
AGGACAGGGGAGTTCTATATAATAATTTTGCTGTATTGAGAACTTCCAATATGACTTCTG
CTTTAGCTGAACTGGCTTTTATATCTAACCCGGAGGAAGAGTCCCTGTTGAAAACAGCCG
ACTTTCGCAGTAAGGCGGCTCAGGCAATTGTTCAGGGTATAAATAATTATTATCGTGATA
TTCAGTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGA

Protein sequence predicted based on nucleotide sequence

ASVASVNGDNINVREGPGTTSDIVGELNKGDSVTVLEKSGDWYKVKLSNGDGWVLSSFLN
LSEQNSDDSADWLYAGAGSNDNQVKPAQTAATAQKEVTLPEWLRPREGKLASAGNSGSTD
NSNNTGSTGNSNNSSDTNNSGSKTGEQPFVWDGGELTKIDVCDNSDEVVVTVYATGKMQL
SAFTIDNPSRLVLDLTGVIPGDIPSTMQVSSNIVQQVRTALYSKEPVKSRVVLDLMKQSG
YKTTLSSDQKTLTVQLDKGQRTVVKSGKKLIAIDPGHGGKDCGAIGCTGLYEKDVTLDVS
RQVVDLLKNSGYDAVLTRTDDTYVGLDERTDYANSLNADLFVSVHINSSEAQTPSGTSTH
YRSEEGKVLSTYIQSALIAGLGRKDRGVLYNNFAVLRTSNMTSALAELAFISNPEEESLL
KTADFRSKAAQAIVQGINNYYRDIQ*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in MCSG-APC100417
Recommended expression in:
Not Applicable