DNASU Plasmid | AP1M2 (H. sapiens )<br>In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: AP1M2

Gene Name: adaptor-related protein complex 1, mu 2 subunit

Original Clone ID:

HR2952A-147-423-14.13

Keyword:

targeted domain

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pET15_NESG

Format:

CLOSED

Source:

Northeast Structural Genomics Consortium

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

Rutgers University
PSI:Biology
Northeast Structural Genomics Consortium

HsCD00590921

Price:

AVAILABLE

No restriction
Special MTA: PSI/NESG

Insert Sequence
Protein Sequence

Insert sequence: 837nts

Open reading frame: 51 to 887

Reference Sequence Alignment

CTTTAAGAAGGAGATATACCATGGGCCATCACCATCACCATCACAGCCATATGTCACGGG
TGCCACCCACTGTCACCAACGCTGTGTCCTGGCGCTCCGAGGGTATCAAGTATAAGAAGA
ACGAGGTCTTCATTGATGTCATAGAGTCTGTCAACCTGCTGGTCAATGCCAACGGCAGCG
TCCTTCTGAGCGAAATCGTCGGTACCATCAAGCTCAAGGTGTTTCTGTCAGGAATGCCAG
AGCTGCGGCTGGGCCTCAATGACCGCGTGCTCTTCGAGCTCACTGGCCGCAGCAAGAACA
AATCAGTAGAGCTGGAGGATGTAAAATTCCACCAGTGCGTGCGGCTCTCTCGCTTTGACA
ACGACCGCACCATCTCCTTCATCCCGCCTGATGGTGACTTTGAGCTCATGTCATACCGCC
TCAGCACCCAGGTCAAGCCACTGATCTGGATTGAGTCTGTCATTGAGAAGTTCTCCCACA
GCCGCGTGGAGATCATGGTCAAGGCCAAGGGGCAGTTTAAGAAACAGTCAGTGGCCAACG
GTGTGGAGATATCTGTGCCTGTACCCAGCGATGCCGACTCCCCCAGATTCAAGACCAGTG
TGGGCAGCGCCAAGTATGTGCCGGAGAGAAACGTCGTGATTTGGAGTATTAAGTCTTTCC
CGGGGGGCAAGGAGTACTTGATGCGAGCCCACTTTGGCCTCCCCAGTGTGGAAAAGGAAG
AGGTGGAGGGCCGGCCCCCCATCGGGGTCAAGTTTGAGATCCCCTACTTCACCGTCTCTG
GGATCCAGGTCCGATACATGAAGATCATTGAGAAAAGTGGTTACCAGGCCCTGCCCTGGG
TTCGCTACATCACCCAGAGTGGCGATTACCAACTTCGTATCAGCTAGCTCGAGATCCGGC
T

Protein sequence predicted based on nucleotide sequence

MSRVPPTVTNAVSWRSEGIKYKKNEVFIDVIESVNLLVNANGSVLLSEIVGTIKLKVFLS
GMPELRLGLNDRVLFELTGRSKNKSVELEDVKFHQCVRLSRFDNDRTISFIPPDGDFELM
SYRLSTQVKPLIWIESVIEKFSHSRVEIMVKAKGQFKKQSVANGVEISVPVPSDADSPRF
KTSVGSAKYVPERNVVIWSIKSFPGGKEYLMRAHFGLPSVEKEEVEGRPPIGVKFEIPYF
TVSGIQVRYMKIIEKSGYQALPWVRYITQSGDYQLRIS*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

c830t,T277I

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinPurified: Not_Tested

ProteinExpressed: Protein_Confirmed

SolubleProtein: Tested_Not_Soluble

Find experimental protocols in HR2952A

Recommended expression in:

Not Applicable

Vector Name:

pET15_NESG

Synonyms:

pET15, pET15-modified, pET15Cc, pET15_NESG

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.

Comments:

Modified Novagen pET vector-common MCS

Size (bp):

5723

Parent Vector:

None

Properties:

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker

Author Name:

Gaetano T. Montleione
Jessica Bearden
Northeast Structural Genomics Consortium

Publications:

PMID: 15808222

Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:

Vector Sequence:

Sequence:

TTCTCATGTTTGACAGCTTATCATCGATAAGCTAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCGAGAAGCTTTGCGTCGACAGAGCTCGCAGGATCCGCAGAATTCGCCATATGGCTGTGATGGTGATGGTGATGGCCCATGGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAA

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI)

327

452

bacterial origin

ori

ColE1 origin of replication

3881

4563

gene fragment

ROP

repressor of primer ROP sequence

3281

3457

primer

T7 forward

T7 forward sequencing primer

481

496

primer

T7 reverse

T7 reverse sequencing primer

257

275

promoter

T7 promoter

478

497

repressor binding site

lacO

lacO regulatory sequence

456

478

selectable marker

AmpR

ampicillin resistance gene

4661

5230

tag

His

N-terminal 6x His tag

387

404

trxn termination sequence

T7 term

T7 termination sequence

257

275

Gene

Gene Symbol :

AP1M2

Symbol Nomenclature :

AP1M2

SYNONYM :

AP1-mu2; HSMU1B; MU-1B; MU1B; mu2

Designation :

Full Nomenclature :

adaptor-related protein complex 1, mu 2 subunit

GENEID :

10053

GI :

9506797

GenBank Accession :

NM_005498

HGNC :

558

MIM :

607309

Vega :

OTTHUMG00000180584

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00590921        ------------------------------------------------------------
NM_005498.4         ATGTCCGCCTCGGCTGTCTTCATTCTGGACGTTAAGGGCAAGCCATTGATCAGCCGCAAC
XM_005259701.1      ATGTCCGCCTCGGCTGTCTTCATTCTGGACGTTAAGGGCAAGCCATTGATCAGCCGCAAC
                                                                                

HsCD00590921        ------------------------------------------------------------
NM_005498.4         TACAAGGGCGATGTGGCCATGAGCAAGATTGAGCACTTCATGCCTTTGCTGGTACAGCGG
XM_005259701.1      TACAAGGGCGATGTGGCCATGAGCAAGATTGAGCACTTCATGCCTTTGCTGGTACAGCGG
                                                                                

HsCD00590921        ------------------------------------------------------------
NM_005498.4         GAGGAGGAAGGCGCCCTGGCCCCGCTGCTGAGCCACGGCCAGGTCCACTTCCTATGGATC
XM_005259701.1      GAGGAGGAAGGCGCCCTGGCCCCGCTGCTGAGCCACGGCCAGGTCCACTTCCTATGGATC
                                                                                

HsCD00590921        ------------------------------------------------------------
NM_005498.4         AAACACAGCAACCTCTACTTGGTGGCCACCACATCGAAGAATGCCAATGCCTCCCTGGTG
XM_005259701.1      AAACACAGCAACCTCTACTTGGTGGCCACCACATCGAAGAATGCCAATGCCTCCCTGGTG
                                                                                

HsCD00590921        ------------------------------------------------------------
NM_005498.4         TACTCCTTCCTGTATAAGACAATAGAGGTATTCTGCGAATACTTCAAGGAGCTGGAGGAG
XM_005259701.1      TACTCCTTCCTGTATAAGACAATAGAGGTATTCTGCGAATACTTCAAGGAGCTGGAGGAG
                                                                                

HsCD00590921        ------------------------------------------------------------
NM_005498.4         GAGAGCATCCGGGACAACTTTGTCATCGTCTACGAGTTGCTGGACGAGCTCATGGACTTT
XM_005259701.1      GAGAGCATCCGGGACAACTTTGTCATCGTCTACGAGTTGCTGGACGAGCTCATGGACTTT
                                                                                

HsCD00590921        -------------------------CTTTAAGAAGGAGATATACCATGGGCCATCACCAT
NM_005498.4         GGCTTCCCGCAGACCACCGACAGCAAGATCCTGCAGGAGTACATCACTCAGCAGAGCAAC
XM_005259701.1      GGCTTCCCGCAGACCACCGACAGCAAGATCCTGCAGGAGTACATCACTCAGCAGAGCAAC
                                             . :*.. ...*...** * **   . ** ..*.* 

HsCD00590921        CACCATCACAGCCATATGTCACGGGTGCCACCCACTGTCACCAACGCTGTGTCCTGGCGC
NM_005498.4         AAGCTGGAGACGGGCAAGTCACGGGTGCCACCCACTGTCACCAACGCTGTGTCCTGGCGC
XM_005259701.1      AAGCTGGAGACGGGCAAGTCACGGGTGCCACCCACTGTCACCAACGCTGTGTCCTGGCGC
                    .* *:  * *   . *:*******************************************

HsCD00590921        TCCGAGGGTATCAAGTATAAGAAGAACGAGGTCTTCATTGATGTCATAGAGTCTGTCAAC
NM_005498.4         TCCGAGGGTATCAAGTATAAGAAGAACGAGGTCTTCATTGATGTCATAGAGTCTGTCAAC
XM_005259701.1      TCCGAGGGTATCAAGTATAAGAAGAACGAGGTCTTCATTGATGTCATAGAGTCTGTCAAC
                    ************************************************************

HsCD00590921        CTGCTGGTCAATGCCAACGGCAGCGTCCTTCTGAGCGAAATCGTCGGTACCATCAAGCTC
NM_005498.4         CTGCTGGTCAATGCCAACGGCAGCGTCCTTCTGAGCGAAATCGTCGGTACCATCAAGCTC
XM_005259701.1      CTGCTGGTCAATGCCAACGGCAGCGTCCTTCTGAGCGAAATCGTCGGTACCATCAAGCTC
                    ************************************************************

HsCD00590921        AAGGTGTTTCTGTCAGGAATGCCAGAGCTGCGGCTGGGCCTCAATGACCGCGTGCTCTTC
NM_005498.4         AAGGTGTTTCTGTCAGGAATGCCAGAGCTGCGGCTGGGCCTCAATGACCGCGTGCTCTTC
XM_005259701.1      AAGGTGTTTCTGTCAGGAATGCCAGAGCTGCGGCTGGGCCTCAATGACCGCGTGCTCTTC
                    ************************************************************

HsCD00590921        GAGCTCACTG------GCCGCAGCAAGAACAAATCAGTAGAGCTGGAGGATGTAAAATTC
NM_005498.4         GAGCTCACTG------GCCGCAGCAAGAACAAATCAGTAGAGCTGGAGGATGTAAAATTC
XM_005259701.1      GAGCTCACTGGCCTTTCAGGCAGCAAGAACAAATCAGTAGAGCTGGAGGATGTAAAATTC
                    **********       . *****************************************

HsCD00590921        CACCAGTGCGTGCGGCTCTCTCGCTTTGACAACGACCGCACCATCTCCTTCATCCCGCCT
NM_005498.4         CACCAGTGCGTGCGGCTCTCTCGCTTTGACAACGACCGCACCATCTCCTTCATCCCGCCT
XM_005259701.1      CACCAGTGCGTGCGGCTCTCTCGCTTTGACAACGACCGCACCATCTCCTTCATCCCGCCT
                    ************************************************************

HsCD00590921        GATGGTGACTTTGAGCTCATGTCATACCGCCTCAGCACCCAGGTCAAGCCACTGATCTGG
NM_005498.4         GATGGTGACTTTGAGCTCATGTCATACCGCCTCAGCACCCAGGTCAAGCCACTGATCTGG
XM_005259701.1      GATGGTGACTTTGAGCTCATGTCATACCGCCTCAGCACCCAGGTCAAGCCACTGATCTGG
                    ************************************************************

HsCD00590921        ATTGAGTCTGTCATTGAGAAGTTCTCCCACAGCCGCGTGGAGATCATGGTCAAGGCCAAG
NM_005498.4         ATTGAGTCTGTCATTGAGAAGTTCTCCCACAGCCGCGTGGAGATCATGGTCAAGGCCAAG
XM_005259701.1      ATTGAGTCTGTCATTGAGAAGTTCTCCCACAGCCGCGTGGAGATCATGGTCAAGGCCAAG
                    ************************************************************

HsCD00590921        GGGCAGTTTAAGAAACAGTCAGTGGCCAACGGTGTGGAGATATCTGTGCCTGTACCCAGC
NM_005498.4         GGGCAGTTTAAGAAACAGTCAGTGGCCAACGGTGTGGAGATATCTGTGCCTGTACCCAGC
XM_005259701.1      GGGCAGTTTAAGAAACAGTCAGTGGCCAACGGTGTGGAGATATCTGTGCCTGTACCCAGC
                    ************************************************************

HsCD00590921        GATGCCGACTCCCCCAGATTCAAGACCAGTGTGGGCAGCGCCAAGTATGTGCCGGAGAGA
NM_005498.4         GATGCCGACTCCCCCAGATTCAAGACCAGTGTGGGCAGCGCCAAGTATGTGCCGGAGAGA
XM_005259701.1      GATGCCGACTCCCCCAGATTCAAGACCAGTGTGGGCAGCGCCAAGTATGTGCCGGAGAGA
                    ************************************************************

HsCD00590921        AACGTCGTGATTTGGAGTATTAAGTCTTTCCCGGGGGGCAAGGAGTACTTGATGCGAGCC
NM_005498.4         AACGTCGTGATTTGGAGTATTAAGTCTTTCCCGGGGGGCAAGGAGTACTTGATGCGAGCC
XM_005259701.1      AACGTCGTGATTTGGAGTATTAAGTCTTTCCCGGGGGGCAAGGAGTACTTGATGCGAGCC
                    ************************************************************

HsCD00590921        CACTTTGGCCTCCCCAGTGTGGAAAAGGAAGAGGTGGAGGGCCGGCCCCCCATCGGGGTC
NM_005498.4         CACTTTGGCCTCCCCAGTGTGGAAAAGGAAGAGGTGGAGGGCCGGCCCCCCATCGGGGTC
XM_005259701.1      CACTTTGGCCTCCCCAGTGTGGAAAAGGAAGAGGTGGAGGGCCGGCCCCCCATCGGGGTC
                    ************************************************************

HsCD00590921        AAGTTTGAGATCCCCTACTTCACCGTCTCTGGGATCCAGGTCCGATACATGAAGATCATT
NM_005498.4         AAGTTTGAGATCCCCTACTTCACCGTCTCTGGGATCCAGGTCCGATACATGAAGATCATT
XM_005259701.1      AAGTTTGAGATCCCCTACTTCACCGTCTCTGGGATCCAGGTCCGATACATGAAGATCATT
                    ************************************************************

HsCD00590921        GAGAAAAGTGGTTACCAGGCCCTGCCCTGGGTTCGCTACATCACCCAGAGTGGCGATTAC
NM_005498.4         GAGAAAAGTGGTTACCAGGCCCTGCCCTGGGTTCGCTACATCACCCAGAGTGGCGATTAC
XM_005259701.1      GAGAAAAGTGGTTACCAGGCCCTGCCCTGGGTTCGCTACATCACCCAGAGTGGCGATTAC
                    ************************************************************

HsCD00590921        CAACTTCGTATCAGCTAGCTCGAGATCCGGCT
NM_005498.4         CAACTTCGTACCAGCTAG--------------
XM_005259701.1      CAACTTCGTACCAGCTAG--------------
                    ********** *******

Pathways

Reactome :

Adaptive Immune System

Reactome :

Clathrin derived vesicle budding

Reactome :

Disease

Reactome :

Golgi Associated Vesicle Biogenesis

Reactome :

HIV Infection

Reactome :

Host Interactions of HIV factors

Reactome :

Immune System

Reactome :

Lysosome Vesicle Biogenesis

Reactome :

Membrane Trafficking

Reactome :

MHC class II antigen presentation

Reactome :

Nef mediated downregulation of MHC class I complex cell surface expression

Reactome :

Nef-mediates down modulation of cell surface receptors by recruiting them to clathrin adapters

Reactome :

The role of Nef in HIV-1 replication and disease pathogenesis

Reactome :

trans-Golgi Network Vesicle Budding

Protein

Structural Annotation Wiki :

HR2952A

UniProt :

Q9Y6Q5

HPRD :

06296

Clone

Cloning Information :

627214

Original Clone ID :

HR2952A-147-423-14.13

Experimental

TargetTrack - Experimental data :

HR2952A

Organism

Species Specific ID:

10053

Genome

Chromosome :

Map Location :

19p13.2

Ensembl :

ENSG00000129354

Reagents

Labome :

AP1M2-antibody

AP1M2 (H. sapiens )In pET15_NESG (His-tagged bacterial expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Pathways

Protein

Clone

Experimental

Organism

Genome

Reagents

AP1M2 (H. sapiens )
In pET15_NESG (His-tagged bacterial expression vector)