DNASU Plasmid | COPS3 (H. sapiens )<br>In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: COPS3

Gene Name: COP9 signalosome subunit 3

Original Clone ID:

HR3016-1-411-14.6

Keyword:

full length protein truncation

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pET15_NESG

Format:

CLOSED

Source:

Northeast Structural Genomics Consortium

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

Rutgers University
PSI:Biology
Northeast Structural Genomics Consortium

HsCD00590940

Price:

AVAILABLE

No restriction
Special MTA: PSI/NESG

Insert Sequence
Protein Sequence

Insert sequence: 1236nts

Open reading frame: 26 to 1261

Reference Sequence Alignment

CCATCACCATCACCATCACAGCCATATGGCGTCTGCCCTGGAGCAGTTCGTGAACAGTGT
CCGACAGCTCTCAGCTCAAGGGCAAATGACACAGCTTTGTGAACTGATCAACAAGAGTGG
GGAACTCCTTGCGAAGAACTTATCCCATCTGGACACTGTGCTCGGGGCTCTGGATGTACA
AGAACACTCCTTGGGCGTCCTTGCTGTTTTGTTTGTGAAGTTTTCTATGCCCAGTGTTCC
TGACTTCGAAACGCTATTCTCACAGGTTCAGCTCTTCATCAGCACTTGTAATGGGGAGCA
CATTCGATATGCAACAGACACTTTTGCTGGGCTTTGCCATCAGCTAACAAATGCACTTGT
GGAAAGAAAACAGCCCCTGCGAGGAATTGGCATCCTTAAGCAAGCCATAGACAAGATGCA
GATGAATACAAACCAGCTGACCTCAATACATGCTGATCTCTGCCAGCTTTGTTTGCTAGC
AAAATGCTTTAAGCCTGCCCTTCCATATCTTGACGTGGATATGATGGATATCTGTAAAGA
GAATGGAGCCTATGATGCAAAACACTTTTTATGTTACTATTATTATGGAGGGATGATCTA
TACTGGGCTGAAGAACTTTGAAAGAGCTCTCTACTTTTATGAACAGGCTATAACTACTCC
TGCCATGGCGGTCAGTCATATCATGTTGGAATCATATAAAAAGTATATTTTAGTGTCTTT
GATATTACTTGGCAAAGTACAACAGCTACCAAAATATACATCTCAAATTGTGGGTAGATT
CATTAAGCCTCTTAGCAATGCATACCACGAGTTAGCACAAGTGTATTCAACCAACAACCC
CTCAGAACTCCGAAACCTGGTGAATAAGCACAGTGAAACCTTCACTCGCGATAACAACAT
GGGGCTGGTGAAGCAATGCTTGTCATCTCTTTATAAGAAGAATATTCAGAGGCTAACAAA
GACCTTTTTAACTCTATCATTACAAGATATGGCAAGTCGTGTGCAGTTGTCTGGACCTCA
GGAGGCAGAGAAATACGTTCTGCACATGATAGAAGATGGTGAGATTTTTGCAAGTATTAA
CCAGAAGGACGGTATGGTCAGTTTCCATGATAACCCTGAAAAATATAATAACCCAGCCAT
GCTTCATAACATTGATCAGGAGATGCTGAAGTGCATTGAGCTGGATGAGCGGCTGAAAGC
CATGGACCAGGAGTTCACAGTGAACCCTCAGTTTGTACAAAAGAGTATGGGCTCACAATA
G

Protein sequence predicted based on nucleotide sequence

MASALEQFVNSVRQLSAQGQMTQLCELINKSGELLAKNLSHLDTVLGALDVQEHSLGVLA
VLFVKFSMPSVPDFETLFSQVQLFISTCNGEHIRYATDTFAGLCHQLTNALVERKQPLRG
IGILKQAIDKMQMNTNQLTSIHADLCQLCLLAKCFKPALPYLDVDMMDICKENGAYDAKH
FLCYYYYGGMIYTGLKNFERALYFYEQAITTPAMAVSHIMLESYKKYILVSLILLGKVQQ
LPKYTSQIVGRFIKPLSNAYHELAQVYSTNNPSELRNLVNKHSETFTRDNNMGLVKQCLS
SLYKKNIQRLTKTFLTLSLQDMASRVQLSGPQEAEKYVLHMIEDGEIFASINQKDGMVSF
HDNPEKYNNPAMLHNIDQEMLKCIELDERLKAMDQEFTVNPQFVQKSMGSQ*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

t573c,I191I;a1189t,I397F

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinExpressed: Protein_Confirmed

SolubleProtein: Protein_Soluble

ProteinPurified: Tested_Not_Purified

Find experimental protocols in HR3016

Recommended expression in:

Not Applicable

Vector Name:

pET15_NESG

Synonyms:

pET15, pET15-modified, pET15Cc, pET15_NESG

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.

Comments:

Modified Novagen pET vector-common MCS

Size (bp):

5723

Parent Vector:

None

Properties:

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker

Author Name:

Gaetano T. Montleione
Jessica Bearden
Northeast Structural Genomics Consortium

Publications:

PMID: 15808222

Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:

Vector Sequence:

Sequence:

TTCTCATGTTTGACAGCTTATCATCGATAAGCTAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCGAGAAGCTTTGCGTCGACAGAGCTCGCAGGATCCGCAGAATTCGCCATATGGCTGTGATGGTGATGGTGATGGCCCATGGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAA

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI)

327

452

bacterial origin

ori

ColE1 origin of replication

3881

4563

gene fragment

ROP

repressor of primer ROP sequence

3281

3457

primer

T7 forward

T7 forward sequencing primer

481

496

primer

T7 reverse

T7 reverse sequencing primer

257

275

promoter

T7 promoter

478

497

repressor binding site

lacO

lacO regulatory sequence

456

478

selectable marker

AmpR

ampicillin resistance gene

4661

5230

tag

His

N-terminal 6x His tag

387

404

trxn termination sequence

T7 term

T7 termination sequence

257

275

Gene

Gene Symbol :

COPS3

Symbol Nomenclature :

COPS3

SYNONYM :

CSN3; SGN3

Designation :

COP9 complex subunit 3|COP9 constitutive photomorphogenic homolog subunit 3|COP9 signalosome complex subunit 3|JAB1-containing signalosome subunit 3|signalosome subunit 3

Full Nomenclature :

COP9 signalosome subunit 3

GENEID :

8533

GI :

23238222

GenBank Accession :

NM_003653

HGNC :

2239

MIM :

604665

Vega :

OTTHUMG00000059281

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00590940        CCATCACCATCACCATCACAGCCATATGGCGTCTGCCCTGGAGCAGTTCGTGAACAGTGT
NM_001199125.1      ------------------------------------------------------------
NM_003653.3         -------------------------ATGGCGTCTGCCCTGGAGCAGTTCGTGAACAGTGT
                                                                                

HsCD00590940        CCGACAGCTCTCAGCTCAAGGGCAAATGACACAGCTTTGTGAACTGATCAACAAGAGTGG
NM_001199125.1      -------------------------ATGACACAGCTTTGTGAACTGATCAACAAGAGTGG
NM_003653.3         CCGACAGCTCTCAGCTCAAGGGCAAATGACACAGCTTTGTGAACTGATCAACAAGAGTGG
                                             ***********************************

HsCD00590940        GGAACTCCTTGCGAAGAACTTATCCCATCTGGACACTGTGCTCGGGGCTCTGGATGTACA
NM_001199125.1      GGAACTCCTTGCGAAGAACTTATCCCATCTGGACACTGTGCTCGGGGCTCTGGATGTACA
NM_003653.3         GGAACTCCTTGCGAAGAACTTATCCCATCTGGACACTGTGCTCGGGGCTCTGGATGTACA
                    ************************************************************

HsCD00590940        AGAACACTCCTTGGGCGTCCTTGCTGTTTTGTTTGTGAAGTTTTCTATGCCCAGTGTTCC
NM_001199125.1      AGAACACTCCTTGGGCGTCCTTGCTGTTTTGTTTGTGAAGTTTTCTATGCCCAGTGTTCC
NM_003653.3         AGAACACTCCTTGGGCGTCCTTGCTGTTTTGTTTGTGAAGTTTTCTATGCCCAGTGTTCC
                    ************************************************************

HsCD00590940        TGACTTCGAAACGCTATTCTCACAGGTTCAGCTCTTCATCAGCACTTGTAATGGGGAGCA
NM_001199125.1      TGACTTCGAAACGCTATTCTCACAGGTTCAGCTCTTCATCAGCACTTGTAATGGGGAGCA
NM_003653.3         TGACTTCGAAACGCTATTCTCACAGGTTCAGCTCTTCATCAGCACTTGTAATGGGGAGCA
                    ************************************************************

HsCD00590940        CATTCGATATGCAACAGACACTTTTGCTGGGCTTTGCCATCAGCTAACAAATGCACTTGT
NM_001199125.1      CATTCGATATGCAACAGACACTTTTGCTGGGCTTTGCCATCAGCTAACAAATGCACTTGT
NM_003653.3         CATTCGATATGCAACAGACACTTTTGCTGGGCTTTGCCATCAGCTAACAAATGCACTTGT
                    ************************************************************

HsCD00590940        GGAAAGAAAACAGCCCCTGCGAGGAATTGGCATCCTTAAGCAAGCCATAGACAAGATGCA
NM_001199125.1      GGAAAGAAAACAGCCCCTGCGAGGAATTGGCATCCTTAAGCAAGCCATAGACAAGATGCA
NM_003653.3         GGAAAGAAAACAGCCCCTGCGAGGAATTGGCATCCTTAAGCAAGCCATAGACAAGATGCA
                    ************************************************************

HsCD00590940        GATGAATACAAACCAGCTGACCTCAATACATGCTGATCTCTGCCAGCTTTGTTTGCTAGC
NM_001199125.1      GATGAATACAAACCAGCTGACCTCAATACATGCTGATCTCTGCCAGCTTTGTTTGCTAGC
NM_003653.3         GATGAATACAAACCAGCTGACCTCAATACATGCTGATCTCTGCCAGCTTTGTTTGCTAGC
                    ************************************************************

HsCD00590940        AAAATGCTTTAAGCCTGCCCTTCCATATCTTGACGTGGATATGATGGATATCTGTAAAGA
NM_001199125.1      AAAATGCTTTAAGCCTGCCCTTCCATATCTTGACGTGGATATGATGGATATCTGTAAAGA
NM_003653.3         AAAATGCTTTAAGCCTGCCCTTCCATATCTTGACGTGGATATGATGGATATCTGTAAAGA
                    ************************************************************

HsCD00590940        GAATGGAGCCTATGATGCAAAACACTTTTTATGTTACTATTATTATGGAGGGATGATCTA
NM_001199125.1      GAATGGAGCCTATGATGCAAAACACTTTTTATGTTACTATTATTATGGAGGGATGATCTA
NM_003653.3         GAATGGAGCCTATGATGCAAAACACTTTTTATGTTACTATTATTATGGAGGGATGATCTA
                    ************************************************************

HsCD00590940        TACTGGGCTGAAGAACTTTGAAAGAGCTCTCTACTTTTATGAACAGGCTATAACTACTCC
NM_001199125.1      TACTGGGCTGAAGAACTTTGAAAGAGCTCTCTACTTTTATGAACAGGCTATAACTACTCC
NM_003653.3         TACTGGGCTGAAGAACTTTGAAAGAGCTCTCTACTTTTATGAACAGGCTATAACTACTCC
                    ************************************************************

HsCD00590940        TGCCATGGCGGTCAGTCATATCATGTTGGAATCATATAAAAAGTATATTTTAGTGTCTTT
NM_001199125.1      TGCCATGGCGGTCAGTCATATCATGTTGGAATCATATAAAAAGTATATTTTAGTGTCTTT
NM_003653.3         TGCCATGGCGGTCAGTCATATCATGTTGGAATCATATAAAAAGTATATTTTAGTGTCTTT
                    ************************************************************

HsCD00590940        GATATTACTTGGCAAAGTACAACAGCTACCAAAATATACATCTCAAATTGTGGGTAGATT
NM_001199125.1      GATATTACTTGGCAAAGTACAACAGCTACCAAAATATACATCTCAAATTGTGGGTAGATT
NM_003653.3         GATATTACTTGGCAAAGTACAACAGCTACCAAAATATACATCTCAAATTGTGGGTAGATT
                    ************************************************************

HsCD00590940        CATTAAGCCTCTTAGCAATGCATACCACGAGTTAGCACAAGTGTATTCAACCAACAACCC
NM_001199125.1      CATTAAGCCTCTTAGCAATGCATACCACGAGTTAGCACAAGTGTATTCAACCAACAACCC
NM_003653.3         CATTAAGCCTCTTAGCAATGCATACCACGAGTTAGCACAAGTGTATTCAACCAACAACCC
                    ************************************************************

HsCD00590940        CTCAGAACTCCGAAACCTGGTGAATAAGCACAGTGAAACCTTCACTCGCGATAACAACAT
NM_001199125.1      CTCAGAACTCCGAAACCTGGTGAATAAGCACAGTGAAACCTTCACTCGCGATAACAACAT
NM_003653.3         CTCAGAACTCCGAAACCTGGTGAATAAGCACAGTGAAACCTTCACTCGCGATAACAACAT
                    ************************************************************

HsCD00590940        GGGGCTGGTGAAGCAATGCTTGTCATCTCTTTATAAGAAGAATATTCAGAGGCTAACAAA
NM_001199125.1      GGGGCTGGTGAAGCAATGCTTGTCATCTCTTTATAAGAAGAATATTCAGAGGCTAACAAA
NM_003653.3         GGGGCTGGTGAAGCAATGCTTGTCATCTCTTTATAAGAAGAATATTCAGAGGCTAACAAA
                    ************************************************************

HsCD00590940        GACCTTTTTAACTCTATCATTACAAGATATGGCAAGTCGTGTGCAGTTGTCTGGACCTCA
NM_001199125.1      GACCTTTTTAACTCTATCATTACAAGATATGGCAAGTCGTGTGCAGTTGTCTGGACCTCA
NM_003653.3         GACCTTTTTAACTCTATCATTACAAGATATGGCAAGTCGTGTGCAGTTGTCTGGACCTCA
                    ************************************************************

HsCD00590940        GGAGGCAGAGAAATACGTTCTGCACATGATAGAAGATGGTGAGATTTTTGCAAGTATTAA
NM_001199125.1      GGAGGCAGAGAAATACGTTCTGCACATGATAGAAGATGGTGAGATTTTTGCAAGTATTAA
NM_003653.3         GGAGGCAGAGAAATACGTTCTGCACATGATAGAAGATGGTGAGATTTTTGCAAGTATTAA
                    ************************************************************

HsCD00590940        CCAGAAGGACGGTATGGTCAGTTTCCATGATAACCCTGAAAAATATAATAACCCAGCCAT
NM_001199125.1      CCAGAAGGACGGTATGGTCAGTTTCCATGATAACCCTGAAAAATATAATAACCCAGCCAT
NM_003653.3         CCAGAAGGACGGTATGGTCAGTTTCCATGATAACCCTGAAAAATATAATAACCCAGCCAT
                    ************************************************************

HsCD00590940        GCTTCATAACATTGATCAGGAGATGCTGAAGTGCATTGAGCTGGATGAGCGGCTGAAAGC
NM_001199125.1      GCTTCATAACATTGATCAGGAGATGCTGAAGTGCATTGAGCTGGATGAGCGGCTGAAAGC
NM_003653.3         GCTTCATAACATTGATCAGGAGATGCTGAAGTGCATTGAGCTGGATGAGCGGCTGAAAGC
                    ************************************************************

HsCD00590940        CATGGACCAGGAGTTCACAGTGAACCCTCAGTTTGTACAAAAGAGTATGGGCTCACAATA
NM_001199125.1      CATGGACCAGGAGATCACAGTGAACCCTCAGTTTGTACAAAAGAGTATGGGCTCACAAGA
NM_003653.3         CATGGACCAGGAGATCACAGTGAACCCTCAGTTTGTACAAAAGAGTATGGGCTCACAAGA
                    *************:******************************************** *

HsCD00590940        G------------------------------------
NM_001199125.1      AGATGATTCAGGAAACAAACCATCCAGTTATTCTTGA
NM_003653.3         AGATGATTCAGGAAACAAACCATCCAGTTATTCTTGA
                    .