DNASU Plasmid | FBXL5 (H. sapiens )<br>In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: FBXL5

Gene Name: F-box and leucine-rich repeat protein 5

Original Clone ID:

HR3547-205-691-14.5

Keyword:

targeted domain

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pET15_NESG

Format:

CLOSED

Source:

Northeast Structural Genomics Consortium

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

Rutgers University
PSI:Biology
Northeast Structural Genomics Consortium

HsCD00590993

Price:

AVAILABLE

No restriction
Special MTA: PSI/NESG

Insert Sequence
Protein Sequence

Insert sequence: 1467nts

Open reading frame: 12 to 1478

Reference Sequence Alignment

ATCACAGCCATATGATAACCCATCTTCCTCCTGAGGTAATGCTGTCAATTTTCAGCTATC
TTAATCCTCAAGAGTTATGTCGATGCAGTCAAGTAAGCATGAAATGGTCTCAGCTGACAA
AAACGGGATCGCTTTGGAAACATCTTTACCCTGTTCATTGGGCCAGAGGTGACTGGTATA
GTGGTCCCGCAACTGAACTTGATACTGAACCTGATGATGAATGGGTGAAAAATAGGAAAG
ATGAAAGTCGTGCTTTTCATGAGTGGGATGAAGATGCTGACATTGATGAATCTGAAGAGT
CTGCGGAGGAATCAATTGCTATCAGCATTGCACAAATGGAAAAACGTTTACTCCATGGCT
TAATTCATAACGTTCTACCATATGTTGGTACTTCTGTAAAAACCTTAGTATTAGCATACA
GCTCTGCAGTTTCCAGCAAAATGGTTAGGCAGATTTTAGAGCTTTGTCCTAACCTGGAGC
ATCTGGATCTTACCCAGACTGACATTTCAGATTCTGCATTTGACAGTTGGTCTTGGCTTG
GTTGCTGCCAGAGTCTTCGGCATCTTGATCTGTCTGGTTGTGAGAAAATCACAGATGTGG
CCCTAGAGAAGATTTCCAGAGCTCTTGGAATTCTGACATCTCATCAAAGTGGCTTTTTGA
AAACATCTACAAGCAAAATTACTTCAACTGCGTGGAAAAATAAAGACATTACCATGCAGT
CCACCAAGCAGTATGCCTGTTTGCACGATTTAACTAACAAGGGCATTGGAGAAGAAATAG
ATAATGAACACCCCTGGACTAAGCCTGTTTCTTCTGAGAATTTCACTTCTCCTTATGTGT
GGATGTTAGATGCTGAAGATTTGGCTGATATTGAAGATACTGTGGAATGGAGACATAGAA
ATGTTGAAAGTCTTTGTGTAATGGAAACAGCATCCAACTTTAGTTGTTCCACCTCTGGTT
GTTTTAGTAAGGACATTGTTGGACTAAGGACTAGTGTCTGTTGGCAGCAGCATTGTGCTT
CTCCAGCCTTTGCGTATTGTGGTCACTCATTTTGTTGTACAGGAACAGCTTTAAGAACTA
TGTCATCACTCCCAGAATCTTCTGCAATGTGTAGAAAAGCAGCAAGGACTAGATTGCCTA
GGGGAAAAGACTTAATTTACTTTGGGAGTGAAAAATCTGATCAAGAGACTGGACGTGTAC
TTCTGTTTCTCAGTTTATCTGGATGTTATCAGATCACAGACCATGGTCTCAGGATTTTGA
CTCTGGGAGGAGGGCTGCCTTATTTGGAGCACCTTAATCTCTCTGGTTGTCTTACTATAA
CTGGTGCAGGCCTGCAGGATTTGGTTTCAGCATGTCCTTCTCTGAATGATGAATACTTTT
ACTACTGTGACAACATTAACGGTCCTCATGCTGATACCGCCAGTGGATGCCAGAATTTGC
AGTGTGGTTTTCGAGCCTGCTGCCGCTCTGGCGAATAG

Protein sequence predicted based on nucleotide sequence

MITHLPPEVMLSIFSYLNPQELCRCSQVSMKWSQLTKTGSLWKHLYPVHWARGDWYSGPA
TELDTEPDDEWVKNRKDESRAFHEWDEDADIDESEESAEESIAISIAQMEKRLLHGLIHN
VLPYVGTSVKTLVLAYSSAVSSKMVRQILELCPNLEHLDLTQTDISDSAFDSWSWLGCCQ
SLRHLDLSGCEKITDVALEKISRALGILTSHQSGFLKTSTSKITSTAWKNKDITMQSTKQ
YACLHDLTNKGIGEEIDNEHPWTKPVSSENFTSPYVWMLDAEDLADIEDTVEWRHRNVES
LCVMETASNFSCSTSGCFSKDIVGLRTSVCWQQHCASPAFAYCGHSFCCTGTALRTMSSL
PESSAMCRKAARTRLPRGKDLIYFGSEKSDQETGRVLLFLSLSGCYQITDHGLRILTLGG
GLPYLEHLNLSGCLTITGAGLQDLVSACPSLNDEYFYYCDNINGPHADTASGCQNLQCGF
RACCRSGE*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

g1243a,V415I

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinPurified: Not_Tested

ProteinExpressed: Protein_Confirmed

SolubleProtein: Tested_Not_Soluble

Find experimental protocols in HR3547

Recommended expression in:

Not Applicable

Vector Name:

pET15_NESG

Synonyms:

pET15, pET15-modified, pET15Cc, pET15_NESG

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.

Comments:

Modified Novagen pET vector-common MCS

Size (bp):

5723

Parent Vector:

None

Properties:

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker

Author Name:

Gaetano T. Montleione
Jessica Bearden
Northeast Structural Genomics Consortium

Publications:

PMID: 15808222

Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:

Vector Sequence:

Sequence:

TTCTCATGTTTGACAGCTTATCATCGATAAGCTAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCGAGAAGCTTTGCGTCGACAGAGCTCGCAGGATCCGCAGAATTCGCCATATGGCTGTGATGGTGATGGTGATGGCCCATGGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAA

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI)

327

452

bacterial origin

ori

ColE1 origin of replication

3881

4563

gene fragment

ROP

repressor of primer ROP sequence

3281

3457

primer

T7 forward

T7 forward sequencing primer

481

496

primer

T7 reverse

T7 reverse sequencing primer

257

275

promoter

T7 promoter

478

497

repressor binding site

lacO

lacO regulatory sequence

456

478

selectable marker

AmpR

ampicillin resistance gene

4661

5230

tag

His

N-terminal 6x His tag

387

404

trxn termination sequence

T7 term

T7 termination sequence

257

275

Gene

Gene Symbol :

FBXL5

Symbol Nomenclature :

FBXL5

SYNONYM :

FBL4; FBL5; FLR1

Designation :

F-box protein FBL4/FBL5|F-box protein FBL5|F-box/LRR-repeat protein 5|p45SKP2-like protein

Full Nomenclature :

F-box and leucine-rich repeat protein 5

GENEID :

26234

GenBank Accession :

NR_036464

HGNC :

13602

MIM :

605655

Vega :

OTTHUMG00000097097

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00590993        ------------------------------------------------------------
NM_012161.3         ATGGCGCCCTTTCCTGAAGAAGTGGACGTCTTCACCGCCCCACACTGGCGGATGAAGCAG
NM_001193535.1      ATGGCGCCCTTTCCTGAAGAAGTGGACGTCTTCACCGCCCCACACTGGCGGATGAAGCAG
NM_001193534.1      ATGGCGCCCTTTCCTGAAGAAGTGGACGTCTTCACCGCCCCACACTGGCGGATGAAGCAG
                                                                                

HsCD00590993        ------------------------------------------------------------
NM_012161.3         CTGGTGGGGCTCTACTGCGACAAGCTTTCTAAAACCAATTTTTCCAACAACAACGATTTC
NM_001193535.1      CTGGTGGGGCTCTACTGCGACAAG------------------------------------
NM_001193534.1      CTGGTGGGGCTCTACTGCGACAAGCTTTCTAAAACCAATTTTTCCAACAACAACGATTTC
                                                                                

HsCD00590993        ------------------------------------------------------------
NM_012161.3         CGTGCTCTTCTGCAGTCTTTGTATGCTACTTTCAAGGAGTTCAAAATGCATGAGCAGATT
NM_001193535.1      ---------------TCTTTGTATGCTACTTTCAAGGAGTTCAAAATGCATGAGCAGATT
NM_001193534.1      CGTGCTCTTCTGCAGTCTTTGTATGCTACTTTCAAGGAGTTCAAAATGCATGAGCAGATT
                                                                                

HsCD00590993        ------------------------------------------------------------
NM_012161.3         GAAAATGAATACATTATTGGTTTGCTTCAACAACGCAGCCAGACCATTTATAATGTACAT
NM_001193535.1      GAAAATGAATACATTATTGGTTTGCTTCAACAACGCAGCCAGACCATTTATAATGTACAT
NM_001193534.1      GAAAATGAATACATTATTGGTTTGCTTCAACAACGCAGCCAGACCATTTATAATGTACAT
                                                                                

HsCD00590993        ------------------------------------------------------------
NM_012161.3         TCTGACAATAAACTCTCCGAGATGCTTAGCCTCTTTGAAAAGGGACTGAAGAATGTTAAG
NM_001193535.1      TCTGACAATAAACTCTCCGAGATGCTTAGCCTCTTTGAAAAGGGACTGAAGAATGTTAAG
NM_001193534.1      TCTGACAATAAACTCTCCGAGATGCTTAGCCTCTTTGAAAAGGGACTGAAGAATGTTAAG
                                                                                

HsCD00590993        ------------------------------------------------------------
NM_012161.3         AATGAATATGAACAGTTAAATTATGCAAAACAACTGAAAGAGAGATTGGAGGCTTTTACA
NM_001193535.1      AATGAATATGAACAGTTAAATTATGCAAAACAACTGAAAGAGAGATTGGAGGCTTTTACA
NM_001193534.1      AATGAATATGAACAGTTAAATTATGCAAAACAACTGAAAGAGAGATTGGAGGCTTTTACA
                                                                                

HsCD00590993        ------------------------------------------------------------
NM_012161.3         AGAGATTTTCTTCCTCACATGAAAGAGGAAGAGGAGGTTTTTCAGCCCATGTTAATGGAA
NM_001193535.1      AGAGATTTTCTTCCTCACATGAAAGAGGAAGAGGAGGTTTTTCAGCCCATGTTAATGGAA
NM_001193534.1      AGAGATTTTCTTCCTCACATGAAAGAGGAAGAGGAGGTTTTTCAGCCCATGTTAATGGAA
                                                                                

HsCD00590993        ------------------------------------------------------------
NM_012161.3         TATTTTACCTATGAAGAGCTTAAGGATATTAAAAAGAAAGTGATTGCACAACACTGCTCT
NM_001193535.1      TATTTTACCTATGAAGAGCTTAAGGATATTAAAAAGAAAGTGATTGCACAACACTGCTCT
NM_001193534.1      TATTTTACCTATGAAGAGCTTAAGGATATTAAAAAGAAAGTGATTGCACAACACTGCTCT
                                                                                

HsCD00590993        ------------------------------------------------------------
NM_012161.3         CAGAAGGATACTGCAGAACTCCTTAGAGGTCTTAGCCTATGGAATCATGCTGAAGAGCGA
NM_001193535.1      CAGAAGGATACTGCAGAACTCCTTAGAGGTCTTAGCCTATGGAATCATGCTGAAGAGCGA
NM_001193534.1      CAGAAGGATACTGCAGAACTCCTTAGAGGTCTTAGCCTATGGAATCATGCTGAAGAGCGA
                                                                                

HsCD00590993        ----------------------------------------------------------AT
NM_012161.3         CAGAAGTTTTTTAAATATTCCGTGGATGAAAAGTCAGATAAAGAAGCAGAAGTGTCAGAA
NM_001193535.1      CAGAAGTTTTTTAAATATTCCGTGGATGAAAAGTCAGATAAAGAAGCAGAAGTGTCAGAA
NM_001193534.1      CAGAAGTTTTTTAAATATTCCGTGGATGAAAAGTCAGATAAAGCAGAAGTG---TCAGAA
                                                                              *:

HsCD00590993        CACAGCCATATGATAACCCATCTTCCTCCTGAGGTAATGCTGTCAATTTTCAGCTATCTT
NM_012161.3         CACTCCACAGGTATAACCCATCTTCCTCCTGAGGTAATGCTGTCAATTTTCAGCTATCTT
NM_001193535.1      CACTCCACAGGTATAACCCATCTTCCTCCTGAGGTAATGCTGTCAATTTTCAGCTATCTT
NM_001193534.1      CACTCCACAGGTATAACCCATCTTCCTCCTGAGGTAATGCTGTCAATTTTCAGCTATCTT
                    ***: *..:.  ************************************************

HsCD00590993        AATCCTCAAGAGTTATGTCGATGCAGTCAAGTAAGCATGAAATGGTCTCAGCTGACAAAA
NM_012161.3         AATCCTCAAGAGTTATGTCGATGCAGTCAAGTAAGCATGAAATGGTCTCAGCTGACAAAA
NM_001193535.1      AATCCTCAAGAGTTATGTCGATGCAGTCAAGTAAGCATGAAATGGTCTCAGCTGACAAAA
NM_001193534.1      AATCCTCAAGAGTTATGTCGATGCAGTCAAGTAAGCATGAAATGGTCTCAGCTGACAAAA
                    ************************************************************

HsCD00590993        ACGGGATCGCTTTGGAAACATCTTTACCCTGTTCATTGGGCCAGAGGTGACTGGTATAGT
NM_012161.3         ACGGGATCGCTTTGGAAACATCTTTACCCTGTTCATTGGGCCAGAGGTGACTGGTATAGT
NM_001193535.1      ACGGGATCGCTTTGGAAACATCTTTACCCTGTTCATTGGGCCAGAGGTGACTGGTATAGT
NM_001193534.1      ACGGGATCGCTTTGGAAACATCTTTACCCTGTTCATTGGGCCAGAGGTGACTGGTATAGT
                    ************************************************************

HsCD00590993        GGTCCCGCAACTGAACTTGATACTGAACCTGATGATGAATGGGTGAAAAATAGGAAAGAT
NM_012161.3         GGTCCCGCAACTGAACTTGATACTGAACCTGATGATGAATGGGTGAAAAATAGGAAAGAT
NM_001193535.1      GGTCCCGCAACTGAACTTGATACTGAACCTGATGATGAATGGGTGAAAAATAGGAAAGAT
NM_001193534.1      GGTCCCGCAACTGAACTTGATACTGAACCTGATGATGAATGGGTGAAAAATAGGAAAGAT
                    ************************************************************

HsCD00590993        GAAAGTCGTGCTTTTCATGAGTGGGATGAAGATGCTGACATTGATGAATCTGAAGAGTCT
NM_012161.3         GAAAGTCGTGCTTTTCATGAGTGGGATGAAGATGCTGACATTGATGAATCTGAAGAGTCT
NM_001193535.1      GAAAGTCGTGCTTTTCATGAGTGGGATGAAGATGCTGACATTGATGAATCTGAAGAGTCT
NM_001193534.1      GAAAGTCGTGCTTTTCATGAGTGGGATGAAGATGCTGACATTGATGAATCTGAAGAGTCT
                    ************************************************************

HsCD00590993        GCGGAGGAATCAATTGCTATCAGCATTGCACAAATGGAAAAACGTTTACTCCATGGCTTA
NM_012161.3         GCGGAGGAATCAATTGCTATCAGCATTGCACAAATGGAAAAACGTTTACTCCATGGCTTA
NM_001193535.1      GCGGAGGAATCAATTGCTATCAGCATTGCACAAATGGAAAAACGTTTACTCCATGGCTTA
NM_001193534.1      GCGGAGGAATCAATTGCTATCAGCATTGCACAAATGGAAAAACGTTTACTCCATGGCTTA
                    ************************************************************

HsCD00590993        ATTCATAACGTTCTACCATATGTTGGTACTTCTGTAAAAACCTTAGTATTAGCATACAGC
NM_012161.3         ATTCATAACGTTCTACCATATGTTGGTACTTCTGTAAAAACCTTAGTATTAGCATACAGC
NM_001193535.1      ATTCATAACGTTCTACCATATGTTGGTACTTCTGTAAAAACCTTAGTATTAGCATACAGC
NM_001193534.1      ATTCATAACGTTCTACCATATGTTGGTACTTCTGTAAAAACCTTAGTATTAGCATACAGC
                    ************************************************************

HsCD00590993        TCTGCAGTTTCCAGCAAAATGGTTAGGCAGATTTTAGAGCTTTGTCCTAACCTGGAGCAT
NM_012161.3         TCTGCAGTTTCCAGCAAAATGGTTAGGCAGATTTTAGAGCTTTGTCCTAACCTGGAGCAT
NM_001193535.1      TCTGCAGTTTCCAGCAAAATGGTTAGGCAGATTTTAGAGCTTTGTCCTAACCTGGAGCAT
NM_001193534.1      TCTGCAGTTTCCAGCAAAATGGTTAGGCAGATTTTAGAGCTTTGTCCTAACCTGGAGCAT
                    ************************************************************

HsCD00590993        CTGGATCTTACCCAGACTGACATTTCAGATTCTGCATTTGACAGTTGGTCTTGGCTTGGT
NM_012161.3         CTGGATCTTACCCAGACTGACATTTCAGATTCTGCATTTGACAGTTGGTCTTGGCTTGGT
NM_001193535.1      CTGGATCTTACCCAGACTGACATTTCAGATTCTGCATTTGACAGTTGGTCTTGGCTTGGT
NM_001193534.1      CTGGATCTTACCCAGACTGACATTTCAGATTCTGCATTTGACAGTTGGTCTTGGCTTGGT
                    ************************************************************

HsCD00590993        TGCTGCCAGAGTCTTCGGCATCTTGATCTGTCTGGTTGTGAGAAAATCACAGATGTGGCC
NM_012161.3         TGCTGCCAGAGTCTTCGGCATCTTGATCTGTCTGGTTGTGAGAAAATCACAGATGTGGCC
NM_001193535.1      TGCTGCCAGAGTCTTCGGCATCTTGATCTGTCTGGTTGTGAGAAAATCACAGATGTGGCC
NM_001193534.1      TGCTGCCAGAGTCTTCGGCATCTTGATCTGTCTGGTTGTGAGAAAATCACAGATGTGGCC
                    ************************************************************

HsCD00590993        CTAGAGAAGATTTCCAGAGCTCTTGGAATTCTGACATCTCATCAAAGTGGCTTTTTGAAA
NM_012161.3         CTAGAGAAGATTTCCAGAGCTCTTGGAATTCTGACATCTCATCAAAGTGGCTTTTTGAAA
NM_001193535.1      CTAGAGAAGATTTCCAGAGCTCTTGGAATTCTGACATCTCATCAAAGTGGCTTTTTGAAA
NM_001193534.1      CTAGAGAAGATTTCCAGAGCTCTTGGAATTCTGACATCTCATCAAAGTGGCTTTTTGAAA
                    ************************************************************

HsCD00590993        ACATCTACAAGCAAAATTACTTCAACTGCGTGGAAAAATAAAGACATTACCATGCAGTCC
NM_012161.3         ACATCTACAAGCAAAATTACTTCAACTGCGTGGAAAAATAAAGACATTACCATGCAGTCC
NM_001193535.1      ACATCTACAAGCAAAATTACTTCAACTGCGTGGAAAAATAAAGACATTACCATGCAGTCC
NM_001193534.1      ACATCTACAAGCAAAATTACTTCAACTGCGTGGAAAAATAAAGACATTACCATGCAGTCC
                    ************************************************************

HsCD00590993        ACCAAGCAGTATGCCTGTTTGCACGATTTAACTAACAAGGGCATTGGAGAAGAAATAGAT
NM_012161.3         ACCAAGCAGTATGCCTGTTTGCACGATTTAACTAACAAGGGCATTGGAGAAGAAATAGAT
NM_001193535.1      ACCAAGCAGTATGCCTGTTTGCACGATTTAACTAACAAGGGCATTGGAGAAGAAATAGAT
NM_001193534.1      ACCAAGCAGTATGCCTGTTTGCACGATTTAACTAACAAGGGCATTGGAGAAGAAATAGAT
                    ************************************************************

HsCD00590993        AATGAACACCCCTGGACTAAGCCTGTTTCTTCTGAGAATTTCACTTCTCCTTATGTGTGG
NM_012161.3         AATGAACACCCCTGGACTAAGCCTGTTTCTTCTGAGAATTTCACTTCTCCTTATGTGTGG
NM_001193535.1      AATGAACACCCCTGGACTAAGCCTGTTTCTTCTGAGAATTTCACTTCTCCTTATGTGTGG
NM_001193534.1      AATGAACACCCCTGGACTAAGCCTGTTTCTTCTGAGAATTTCACTTCTCCTTATGTGTGG
                    ************************************************************

HsCD00590993        ATGTTAGATGCTGAAGATTTGGCTGATATTGAAGATACTGTGGAATGGAGACATAGAAAT
NM_012161.3         ATGTTAGATGCTGAAGATTTGGCTGATATTGAAGATACTGTGGAATGGAGACATAGAAAT
NM_001193535.1      ATGTTAGATGCTGAAGATTTGGCTGATATTGAAGATACTGTGGAATGGAGACATAGAAAT
NM_001193534.1      ATGTTAGATGCTGAAGATTTGGCTGATATTGAAGATACTGTGGAATGGAGACATAGAAAT
                    ************************************************************

HsCD00590993        GTTGAAAGTCTTTGTGTAATGGAAACAGCATCCAACTTTAGTTGTTCCACCTCTGGTTGT
NM_012161.3         GTTGAAAGTCTTTGTGTAATGGAAACAGCATCCAACTTTAGTTGTTCCACCTCTGGTTGT
NM_001193535.1      GTTGAAAGTCTTTGTGTAATGGAAACAGCATCCAACTTTAGTTGTTCCACCTCTGGTTGT
NM_001193534.1      GTTGAAAGTCTTTGTGTAATGGAAACAGCATCCAACTTTAGTTGTTCCACCTCTGGTTGT
                    ************************************************************

HsCD00590993        TTTAGTAAGGACATTGTTGGACTAAGGACTAGTGTCTGTTGGCAGCAGCATTGTGCTTCT
NM_012161.3         TTTAGTAAGGACATTGTTGGACTAAGGACTAGTGTCTGTTGGCAGCAGCATTGTGCTTCT
NM_001193535.1      TTTAGTAAGGACATTGTTGGACTAAGGACTAGTGTCTGTTGGCAGCAGCATTGTGCTTCT
NM_001193534.1      TTTAGTAAGGACATTGTTGGACTAAGGACTAGTGTCTGTTGGCAGCAGCATTGTGCTTCT
                    ************************************************************

HsCD00590993        CCAGCCTTTGCGTATTGTGGTCACTCATTTTGTTGTACAGGAACAGCTTTAAGAACTATG
NM_012161.3         CCAGCCTTTGCGTATTGTGGTCACTCATTTTGTTGTACAGGAACAGCTTTAAGAACTATG
NM_001193535.1      CCAGCCTTTGCGTATTGTGGTCACTCATTTTGTTGTACAGGAACAGCTTTAAGAACTATG
NM_001193534.1      CCAGCCTTTGCGTATTGTGGTCACTCATTTTGTTGTACAGGAACAGCTTTAAGAACTATG
                    ************************************************************

HsCD00590993        TCATCACTCCCAGAATCTTCTGCAATGTGTAGAAAAGCAGCAAGGACTAGATTGCCTAGG
NM_012161.3         TCATCACTCCCAGAATCTTCTGCAATGTGTAGAAAAGCAGCAAGGACTAGATTGCCTAGG
NM_001193535.1      TCATCACTCCCAGAATCTTCTGCAATGTGTAGAAAAGCAGCAAGGACTAGATTGCCTAGG
NM_001193534.1      TCATCACTCCCAGAATCTTCTGCAATGTGTAGAAAAGCAGCAAGGACTAGATTGCCTAGG
                    ************************************************************

HsCD00590993        GGAAAAGACTTAATTTACTTTGGGAGTGAAAAATCTGATCAAGAGACTGGACGTGTACTT
NM_012161.3         GGAAAAGACTTAATTTACTTTGGGAGTGAAAAATCTGATCAAGAGACTGGACGTGTACTT
NM_001193535.1      GGAAAAGACTTAATTTACTTTGGGAGTGAAAAATCTGATCAAGAGACTGGACGTGTACTT
NM_001193534.1      GGAAAAGACTTAATTTACTTTGGGAGTGAAAAATCTGATCAAGAGACTGGACGTGTACTT
                    ************************************************************

HsCD00590993        CTGTTTCTCAGTTTATCTGGATGTTATCAGATCACAGACCATGGTCTCAGGATTTTGACT
NM_012161.3         CTGTTTCTCAGTTTATCTGGATGTTATCAGATCACAGACCATGGTCTCAGGGTTTTGACT
NM_001193535.1      CTGTTTCTCAGTTTATCTGGATGTTATCAGATCACAGACCATGGTCTCAGGGTTTTGACT
NM_001193534.1      CTGTTTCTCAGTTTATCTGGATGTTATCAGATCACAGACCATGGTCTCAGGGTTTTGACT
                    ***************************************************.********

HsCD00590993        CTGGGAGGAGGGCTGCCTTATTTGGAGCACCTTAATCTCTCTGGTTGTCTTACTATAACT
NM_012161.3         CTGGGAGGAGGGCTGCCTTATTTGGAGCACCTTAATCTCTCTGGTTGTCTTACTATAACT
NM_001193535.1      CTGGGAGGAGGGCTGCCTTATTTGGAGCACCTTAATCTCTCTGGTTGTCTTACTATAACT
NM_001193534.1      CTGGGAGGAGGGCTGCCTTATTTGGAGCACCTTAATCTCTCTGGTTGTCTTACTATAACT
                    ************************************************************

HsCD00590993        GGTGCAGGCCTGCAGGATTTGGTTTCAGCATGTCCTTCTCTGAATGATGAATACTTTTAC
NM_012161.3         GGTGCAGGCCTGCAGGATTTGGTTTCAGCATGTCCTTCTCTGAATGATGAATACTTTTAC
NM_001193535.1      GGTGCAGGCCTGCAGGATTTGGTTTCAGCATGTCCTTCTCTGAATGATGAATACTTTTAC
NM_001193534.1      GGTGCAGGCCTGCAGGATTTGGTTTCAGCATGTCCTTCTCTGAATGATGAATACTTTTAC
                    ************************************************************

HsCD00590993        TACTGTGACAACATTAACGGTCCTCATGCTGATACCGCCAGTGGATGCCAGAATTTGCAG
NM_012161.3         TACTGTGACAACATTAACGGTCCTCATGCTGATACCGCCAGTGGATGCCAGAATTTGCAG
NM_001193535.1      TACTGTGACAACATTAACGGTCCTCATGCTGATACCGCCAGTGGATGCCAGAATTTGCAG
NM_001193534.1      TACTGTGACAACATTAACGGTCCTCATGCTGATACCGCCAGTGGATGCCAGAATTTGCAG
                    ************************************************************

HsCD00590993        TGTGGTTTTCGAGCCTGCTGCCGCTCTGGCGAATAG
NM_012161.3         TGTGGTTTTCGAGCCTGCTGCCGCTCTGGCGAATGA
NM_001193535.1      TGTGGTTTTCGAGCCTGCTGCCGCTCTGGCGAATGA
NM_001193534.1      TGTGGTTTTCGAGCCTGCTGCCGCTCTGGCGAATGA
                    **********************************..

Pathways

Reactome :

Association of TriC/CCT with target proteins during biosynthesis

Reactome :

Chaperonin-mediated protein folding

Reactome :

Metabolism of proteins

Reactome :

Protein folding

Protein

SMART domain :

FBOX : A Receptor for Ubiquitination Targets

SMART domain :

LRR_CC : Leucine-rich repeat - CC (cysteine-containing) subfamily

Structural Annotation Wiki :

HR3547

UniProt :

Q9UKA1

HPRD :

05739

Clone

Cloning Information :

629009

Original Clone ID :

HR3547-205-691-14.5

Experimental

TargetTrack - Experimental data :

HR3547

Organism

Species Specific ID:

26234

Genome

Chromosome :

Map Location :

4p15.32

Ensembl :

ENSG00000118564

Reagents

Labome :

FBXL5-antibody

FBXL5 (H. sapiens )In pET15_NESG (His-tagged bacterial expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Pathways

Protein

Clone

Experimental

Organism

Genome

Reagents

FBXL5 (H. sapiens )
In pET15_NESG (His-tagged bacterial expression vector)