MYO_120650 (S. sp. PCC 6803 )
In pET21_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  MYO_120650
Gene Name:  hypothetical protein
Original Clone ID:
SgR167A-29-105-21.5
Keyword:
targeted domain
Species:
Synechocystis sp. PCC 6803
Type:
cDNA
Vector Name:
pET21_NESG
Format: 
FUSION
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Northeast Structural Genomics Consortium
SsCD00591083
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AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 234nts
Open reading frame: 1 to 234

ATGGAAACCTTGTACACCGTCGGGGGATCGCCGAGGGCTGATATCCGGATCAAATCCCAA
TTGGTTTCCCGTGTCCATGCGGTGGTGGTGTGTAAATCCTCCGACGAGGTACAAGCCGCT
TACCGAATTATTGACGGCGATGAAGATGGACAGAGTAGTGTCAATGGACTGATGATTAAT
GGCAAAAAAGTGCAAGAGCATATCATCCAAACCGGAGACGAAATTGTCATGGGT

Protein sequence predicted based on nucleotide sequence

METLYTVGGSPRADIRIKSQLVSRVHAVVVCKSSDEVQAAYRIIDGDEDGQSSVNGLMIN
GKKVQEHIIQTGDEIVMG

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
del@5,1;c12g,F4L;

a19g,I7V;c25g,R9G;

c48g,R16R;t63g,F21L;

a73g,I25V;c85g,L29V;

c91t,R31C;t108g,D36E
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
SolubleProtein: Not_Tested
ProteinExpressed: Tested_Not_Found
Find experimental protocols in SgR167A
Recommended expression in:
Not Applicable