PP_5086 (P. putida )
In pET21_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  PP_5086
Gene Name:  nuclease
Original Clone ID:
PpR309A-21.1
Keyword:
targeted domain
Species:
Pseudomonas putida
Type:
cDNA
Vector Name:
pET21_NESG
Format: 
FUSION
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Northeast Structural Genomics Consortium
PpCD00591156
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 309nts
Open reading frame: 51 to 359

AATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGGTCGCA
AGGGACGTACCAGTGAGCCCTATGCCGAAGCTGCCAGACGGCGATTGCAGGCCTTGGTCA
AGGCCAATGACGATCGCGTCGGGCTGGTGCCTGGTGTTGAAGGTAAAGACAAGTACGGTC
GCACACTGGCGCACATCTACAGCCGCAATGGCGACAATCTGGAAGCGCAATTGCTTAGCG
AGGGCTTGGGCTACCGCGTGGCTATTGCCCCTAATGTACGTCTCAGCGGTTGTCAGCAGC
TTGCCGAACAAGCCGCGCGCCAGGCCGGCGTTGGCCTGTGGCGGCATTCGCCCGTAGTGC
TCGAGCACCACCACCACCACCACTGAGATCCGGCTGCTAACAAAGCCCGA

Protein sequence predicted based on nucleotide sequence

MGRKGRTSEPYAEAARRRLQALVKANDDRVGLVPGVEGKDKYGRTLAHIYSRNGDNLEAQ
LLSEGLGYRVAIAPNVRLSGCQQLAEQAARQAGVGLWRHSPVV

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
g48a,R16R;t264c,A88A
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
SolubleProtein: Not_Tested
ProteinExpressed: Tested_Not_Found
Find experimental protocols in PpR309A
Recommended expression in:
Not Applicable