CPS_2654 (C. psychrerythraea )
In pET21_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  CPS_2654
Gene Name:  PKD domain-containing protein
Original Clone ID:
CsR136-144-690-21.3
Keyword:
full length protein truncation
Species:
Colwellia psychrerythraea
Type:
cDNA
Vector Name:
pET21_NESG
Format: 
FUSION
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Northeast Structural Genomics Consortium
CpCD00591334
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AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 1644nts
Open reading frame: 41 to 1684

TCCTCTAGATATTTTGTTTACTTTAGAAGGAGATATACATATGCGTGGTGAAAAGCGTAC
CTTTGAAATGCTGACTAATGAAGTTGAATATCAACATTATCGTTTTAGTTTTGCAAACAA
CCCAGCGACCGCTGCAGGCATATTCCAATTAGCTGAAATAGAATTGACTGTTGTGGCAGA
TGCGCCTTTGGTTGATTTTGCAAGTAATATCTCGCGTGCTGAAATAGGTGAAAACGTACA
GTTTTGGGATAGATCTTTAGCTAACCCAACGGGTTGGCAGTGGACATTTGAAGATGGAGA
ACCGGCAACGAGCACGAACAGAAATCCTGCGGTTACTTTCACTTCACTTGGTGCTAAATC
AGTAACACTGGTTGCGTCTAACGATAAAGGTTCTAATGAAAAGGTACAAGAGCAAGTTGT
ACATATTTGGGATTCTCAAAACCCATGGGCAGGTTATGTAAAACCTACCGTTACGCTTGT
TGCTCATAAACCAGAGCATGAAGGGCAAATAGCATTTAGTCGAGTTATGCCTGATATCGA
GGCCGTCATTCATGACATCTCATTACAGATAGTTAAAGTACTGTATAAAGATGTTGCTGA
AGCACCATTATTTAAAACGGTAACCTTTGAAACTGATGAATATGACTTCCCTGCTGCTAA
GAGTGGTACAGATGAAGACATGATCTTAATGATGGATGTTGCTCATCTTGCTAATAAAGC
AGCTGAAGGTGATGATGCCTTGCGCGATGAAGTGATAGGTATGTTATGGCATGAATTAAC
CCATGGTTATAATAATTCGCCAAACTCAGGTGAATACGCAACGGGTGATGAATACCATTC
ATATTTAGAGAGTTTAGCTAACTACATGCGTATTAAAGCCGGCTTTCATGAAAGTAGACG
TGTTGGTATTAAATGGGTTGATAACTGGAATGTTGATGCCTATGAGCAAACATCATTTTT
CTTAGAGTGGGTAGCAAATAGCCATCGTAGCGTTGACTTTATCTATTTATTTAATAAAGC
CGCAGGTGACCTAAAAGAATGGAGTTTTGAGGCCGCCTTTAAATCTATTTTTGGTGAAGA
CAAGGGCGTATCAATATTTTGGCAAGAGTATCATGATGCACAGGGTATTGAACCTCCTTA
TCCAACACCAGTAGATGGCTATAGAAACTTTGCGGTTGATGAAGGTGTGAGCATTTCAAC
TAATGCTACTACCGTTATTATTCCTGATTGGGATGCATATGAAGGGGAAGATAAATTAAT
TGATAATAACGTTACCAAAAAATTTAACGCGTTTATTGAAGGTACATGGTGGCTAGAGGA
ACATGCATCTCACTTATTTCCTATCAATGATATTACCAATGTGGAAGTTACTTTTGAATT
ACCCGATGCCATTGTTTTACATAAATACAGTGTAACAACAGGTAATGATAACCCACAGCG
TGATCCAACGTTATGGACTATATCAGGCTCTGTGGATGGTGAAGTTTGGACTCAATTAGA
TAGCGATAATTATCCTTCTGATCCAGAACGATTAATCACTTTCCATTATGACATTGATGA
TGCAGTGACGGCTTATAAGTATTATCAGTTTGTTTTTGAAAATACTCAACCAGCGGGTGA
TAGTATTGGTGGTGATAATGGTCGCTTGGTTCAAATTGGCGAAATTGCACTACTAACAGA
AGAG

Protein sequence predicted based on nucleotide sequence

MRGEKRTFEMLTNEVEYQHYRFSFANNPATAAGIFQLAEIELTVVADAPLVDFASNISRA
EIGENVQFWDRSLANPTGWQWTFEDGEPATSTNRNPAVTFTSLGAKSVTLVASNDKGSNE
KVQEQVVHIWDSQNPWAGYVKPTVTLVAHKPEHEGQIAFSRVMPDIEAVIHDISLQIVKV
LYKDVAEAPLFKTVTFETDEYDFPAAKSGTDEDMILMMDVAHLANKAAEGDDALRDEVIG
MLWHELTHGYNNSPNSGEYATGDEYHSYLESLANYMRIKAGFHESRRVGIKWVDNWNVDA
YEQTSFFLEWVANSHRSVDFIYLFNKAAGDLKEWSFEAAFKSIFGEDKGVSIFWQEYHDA
QGIEPPYPTPVDGYRNFAVDEGVSISTNATTVIIPDWDAYEGEDKLIDNNVTKKFNAFIE
GTWWLEEHASHLFPINDITNVEVTFELPDAIVLHKYSVTTGNDNPQRDPTLWTISGSVDG
EVWTQLDSDNYPSDPERLITFHYDIDDAVTAYKYYQFVFENTQPAGDSIGGDNGRLVQIG
EIALLTEE

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in CsR136
Recommended expression in:
Not Applicable