PF0933 (P. furiosus )
In pET21_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  PF0933
Gene Name:  DNA repair helicase rad3
Original Clone ID:
PfR191-21.1
Keyword:
None
Species:
Pyrococcus furiosus
Type:
cDNA
Vector Name:
pET21_NESG
Format: 
FUSION
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
Northeast Structural Genomics Consortium
PfCD00591570
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AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 1893nts
Open reading frame: 51 to 1943

GGGGGCGAATTCCTCAGAATATTTTGTTTACTTAAGAAGGAGATATACATATGGAGGAGC
TACAGTACTTTCCCTACGAGAGGTTAAGGCCAAATCAGCGAGAATTTATAGAAATTGTGA
AAGAAGCTGTAAAAAGAGGGGAAAACCTTATTGTTGAAGCTCCCACTGGATTTGGAAAAA
CTATAAGCGTTTTAGCTGGAGTCCTTCCCTATGCAATCTCTTTAGGCTATAAAGTTGTGT
ACCTGGCTAGAACTCATAAGCAGATGGATAGAGTAATAGAGGAGCTCAAAAAAATTAGGG
AAAGGAATGAAGTTAGCGGAATTGAATTCAGGAGTAGAAGGGACTTATGTCTTCACTCCT
ACATCCAAACATTTGTCCAAGATGCCTACACTAGTATGATAGTCTGTAAAACCCTAAGAA
AATTGGGAAAGTGCCCATTCTTTGAAAATATAAAAGAGAAAAGAGACAGAATGGAGGAGA
TAGTGAGATTTTTCCTGAACTCTCCTCCTTTTCCCCATGAGGTTCTCGAATACAGCGAAT
TACTTGAGCTCTGCCCCTACGAAATTACGAAGAAAATTGGAGAGAAAGCTAATGTTATTG
TAGCGAGCTACCTGTATATGATAAGCCCTCCAATAAGGCAGGCTTTCTTAGAAAACTTAG
GTGTGGATTACTCAGATTTGATAGTTATATTTGACGAGGCTCACAACTTACCTGACCAAG
CAATTTCTGCACTTAGTGATAGGCTAAGCATAAGGAGCATAGAGAGGGCCATTAAGGAGG
CCGAAGAGTACGGAGAAAAGGACATTGAGAATTTCCTCTCAATACTCCTAAGGGGATTGG
AGATTCTATACAAAGAAAAGCTGGAAAATTATGAAAGTGAAGAAGTCCCGATAAGGCCAG
AAGAAGTGTTTCTTCACGTAGCTTCCGTTTTGGGATGGAGTGGAAGGGAAATCGGCATGA
TGTTGGAAGATATGATTGAAGTTGGAGATGCAATTAGGGAGGATAGAATAGAGAGAAACC
TCCCCCCTAGAAGCTATGTTGGAAGGGTGGGAGAGTTTCTGTGGAATTGGCTTGCCCTTC
GAGAGAGGGAAGATTACCTTCACCTCTTTACAAAAGAGAGAGGACTTGCGTTGGAGTTAG
TAGCATTAGATCCCTCAGTAGCCCTCGAATTCCTTGAAGAGGTTCACTCTGCAATATTCA
TGTCCGGAACTCTTAGCCCTTTGGAGGCATTTAGAGATATAATAGGAGTTAAAGCAGAGT
TAAAGAAGTTTCCCAGGATGATAAAGAGAGAAAATGCAATAGTTCTTGTGGCGAGAGACG
TGTCTACAAGGGGGATAGAGAGAAGCCCATCCCTTTATAGAAGAATTTCGGAATACATAT
TTGAGGCAGTAAGAAATACCCCAAAAAATGTTGGAGTTTTCACAGCTTCCTATGAAGTTC
TTGAAGGAATTCTAAGCACCAACGTCCACATAAAAATTGAAGAAGAGATAGGGAAAAAGG
TCTTTATAGAGAAGAGAGATGCACCGTCAAGAGAGAATGACGAAATGATAAGAGCATTCA
AAGAAGAATCCAAAGGGAAGGGAGCAGTATTATTTGGCGTTATGGGGGGAAGGAACAGTG
AAGGGCAAGACTACTCCGGAGATGAAATGAACGGTGTTGTCTTAGTTGGGATACCCTATG
CAAGGCCCACACCTAGAGTCCAGGCCCAAATTAGATACTTTGAGAAGAAATTCCCAGGGA
AGGGGAGGTATTATGGGTACGTTTTGCCAGCCCACAGAAAGCTCACCCAAGCTGCGGGGA
GAGTTCATAGGTCTGAAGAAGAGAAGGGAAGCATAGTCATTTTAGACTATAGAGTTTTAT
GGAAAAGTGTTAAGAAAGATCTTCCTGACTGGATGGTTGAGACAATGATTCCTGTAACCC
TCCCCCACTATGAGAATAAAACT

Protein sequence predicted based on nucleotide sequence

MEELQYFPYERLRPNQREFIEIVKEAVKRGENLIVEAPTGFGKTISVLAGVLPYAISLGY
KVVYLARTHKQMDRVIEELKKIRERNEVSGIEFRSRRDLCLHSYIQTFVQDAYTSMIVCK
TLRKLGKCPFFENIKEKRDRMEEIVRFFLNSPPFPHEVLEYSELLELCPYEITKKIGEKA
NVIVASYLYMISPPIRQAFLENLGVDYSDLIVIFDEAHNLPDQAISALSDRLSIRSIERA
IKEAEEYGEKDIENFLSILLRGLEILYKEKLENYESEEVPIRPEEVFLHVASVLGWSGRE
IGMMLEDMIEVGDAIREDRIERNLPPRSYVGRVGEFLWNWLALREREDYLHLFTKERGLA
LELVALDPSVALEFLEEVHSAIFMSGTLSPLEAFRDIIGVKAELKKFPRMIKRENAIVLV
ARDVSTRGIERSPSLYRRISEYIFEAVRNTPKNVGVFTASYEVLEGILSTNVHIKIEEEI
GKKVFIEKRDAPSRENDEMIRAFKEESKGKGAVLFGVMGGRNSEGQDYSGDEMNGVVLVG
IPYARPTPRVQAQIRYFEKKFPGKGRYYGYVLPAHRKLTQAAGRVHRSEEEKGSIVILDY
RVLWKSVKKDLPDWMVETMIPVTLPHYENKT

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
g421a,V141M;t1413c
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
SolubleProtein: Not_Tested
ProteinExpressed: Tested_Not_Found
Find experimental protocols in PfR191
Recommended expression in:
Not Applicable