DNASU Plasmid | PACS1 (H. sapiens )<br>In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: PACS1

Gene Name: phosphofurin acidic cluster sorting protein 1

Original Clone ID:

HR3509D-544-963-15.3

Keyword:

targeted domain

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pET15_NESG

Format:

CLOSED

Source:

Northeast Structural Genomics Consortium

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

Rutgers University
PSI:Biology
Northeast Structural Genomics Consortium

HsCD00596604

Price:

AVAILABLE

No restriction
Special MTA: PSI/NESG

Insert Sequence
Protein Sequence

Insert sequence: 1266nts

Open reading frame: 26 to 1291

Reference Sequence Alignment

CCATCACCATCACCATCACAGCCATATGCCAAGAAAGGTGGTGTATGACCAGCTCAATCA
GATCCTGGTGTCAGATGCAGCCCTCCCAGAAAATGTCATTCTGGTGAACACCACTGACTG
GCAGGGCCAGTATGTGGCTGAGCTGCTCCAGGACCAGCGGAAGCCTGTGGTGTGCACCTG
CTCCACCGTGGAGGTCCAGGCCGTGCTGTCCGCCCTGCTCACCCGGATCCAGCGCTACTG
CAACTGCAACTCTTCCATGCCTAGGCCAGTGAAGGTGGCTGCTGTGGGAGGCCGGAGCTA
CCTGAGCTCCATCCTCAGGTTCTTTGTCAAGTCCCTGGCCAACAAGACCTCCGACTGGCT
TGGCTACATGCGCTTCCTCATCATCCCCCTCGGTTCTCACCCTGAGGCCAAATACTTGGG
GTCAGTCGACAGTAAATACAGTAGTTCCTTCCTGGATTCTGGTTGGAGAGATCTGTTCAG
TCGCTCGGAGCCACCAGTGTCAGAGCAACTGGACGTGGCAGGGCGGGTGATGCAGTACGT
CAACGGGGCAGCCACGACACACCAGCTTCCCGTGGCCGAAGCCATGCTGACTTGCCGGCA
TAAGTTCCCTGATGAAGACTCCTATCAGAAGTTTATTCCCTTCATTGGCGTGGTGAAGGT
GGGTCTGGTTGAAGACTCTCCCTCCACAGCAGGCGATGGGGACGATTCTCCTGTGGTCAG
CCTTACTGTGCCCTCCACATCACCACCCTCCAGCTCGGGCCTGAGCCGAGACGCCACGGC
CACCCCTCCCTCCTCCCCATCTATGAGCAGCGCCCTGGCCATCGTGGGGAGCCCTAATAG
CCCATATGGGGACGTGATTGGCCTCCAGGTGGACTACTGGCTGGGCCACCCCGGGGAGCG
GAGGAGGGAAGGCGACAAGAGGGACGCCAGCTCGAAGAACACCCTCAAGAGTGTCTTCCG
CTCAGTGCAGGTGTCCCGCCTGCCCCATAGCGGGGAGGCCCAGCTTTCTGGCACCATGGC
CATGACTGTGGTCACCAAAGAAAAGAACAAGAAAGTTCCCACCATCTTCCTGAGCAAGAA
ACCCCGAGAAAAGGAGGTGGATTCTAAGAGCCAGGTCATTGAAGGCATCAGCCGCCTCAT
CTGCTCAGCCAAGCAGCAGCAGACTATGCTGAGAGTGTCCATCGATGGGGTCGAGTGGAG
TGACATCCAGTTCTTCCAGCTGGCAGCCCAGTGGCCCACCCATGTCAAGCACTTTCCAGT
GGGACTCTTCAGTGGCAGCAAGGCCACCTAG

Protein sequence predicted based on nucleotide sequence

MPRKVVYDQLNQILVSDAALPENVILVNTTDWQGQYVAELLQDQRKPVVCTCSTVEVQAV
LSALLTRIQRYCNCNSSMPRPVKVAAVGGRSYLSSILRFFVKSLANKTSDWLGYMRFLII
PLGSHPEAKYLGSVDSKYSSSFLDSGWRDLFSRSEPPVSEQLDVAGRVMQYVNGAATTHQ
LPVAEAMLTCRHKFPDEDSYQKFIPFIGVVKVGLVEDSPSTAGDGDDSPVVSLTVPSTSP
PSSSGLSRDATATPPSSPSMSSALAIVGSPNSPYGDVIGLQVDYWLGHPGERRREGDKRD
ASSKNTLKSVFRSVQVSRLPHSGEAQLSGTMAMTVVTKEKNKKVPTIFLSKKPREKEVDS
KSQVIEGISRLICSAKQQQTMLRVSIDGVEWSDIQFFQLAAQWPTHVKHFPVGLFSGSKA
T*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

g237t,P79P;a269g,Q90R;

t380a,V127E;t966c,S322S;

a1183c,K395Q

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinPurified: Not_Tested

SolubleProtein: Not_Tested

ProteinExpressed: Tested_Not_Found

Find experimental protocols in HR3509D

Recommended expression in:

Not Applicable

Vector Name:

pET15_NESG

Synonyms:

pET15, pET15-modified, pET15Cc, pET15_NESG

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.

Comments:

Modified Novagen pET vector-common MCS

Size (bp):

5723

Parent Vector:

None

Properties:

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker

Author Name:

Gaetano T. Montleione
Jessica Bearden
Northeast Structural Genomics Consortium

Publications:

PMID: 15808222

Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:

Vector Sequence:

Sequence:

TTCTCATGTTTGACAGCTTATCATCGATAAGCTAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCGAGAAGCTTTGCGTCGACAGAGCTCGCAGGATCCGCAGAATTCGCCATATGGCTGTGATGGTGATGGTGATGGCCCATGGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAA

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI)

327

452

bacterial origin

ori

ColE1 origin of replication

3881

4563

gene fragment

ROP

repressor of primer ROP sequence

3281

3457

primer

T7 forward

T7 forward sequencing primer

481

496

primer

T7 reverse

T7 reverse sequencing primer

257

275

promoter

T7 promoter

478

497

repressor binding site

lacO

lacO regulatory sequence

456

478

selectable marker

AmpR

ampicillin resistance gene

4661

5230

tag

His

N-terminal 6x His tag

387

404

trxn termination sequence

T7 term

T7 termination sequence

257

275

Gene

Gene Symbol :

PACS1

Symbol Nomenclature :

PACS1

SYNONYM :

MRD17

Designation :

PACS-1|cytosolic sorting protein PACS-1

Full Nomenclature :

phosphofurin acidic cluster sorting protein 1

GENEID :

55690

GI :

30089916

GenBank Accession :

NM_018026

HGNC :

30032

MIM :

607492

Vega :

OTTHUMG00000166889

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00596604      ------------------------------------------------------------
NM_018026.3       ATGGCGGAACGCGGAGGGGCGGGCGGTGGTCCCGGAGGCGCCGGGGGCGGCAGCGGCCAG
                                                                              

HsCD00596604      ------------------------------------------------------------
NM_018026.3       CGGGGATCCGGGGTCGCCCAGTCCCCTCAGCAGCCGCCGCCGCAGCAGCAGCAGCAGCAG
                                                                              

HsCD00596604      ------------------------------------------------------------
NM_018026.3       CCGCCGCAGCAGCCGACGCCCCCCAAGCTGGCCCAGGCCACCTCGTCGTCCTCGTCCACC
                                                                              

HsCD00596604      ------------------------------------------------------------
NM_018026.3       TCGGCGGCGGCTGCCTCCTCCTCGTCCTCGTCTACCTCCACCTCCATGGCCGTGGCGGTG
                                                                              

HsCD00596604      ------------------------------------------------------------
NM_018026.3       GCCTCGGGCTCCGCGCCTCCCGGTGGCCCGGGGCCAGGCCGCACCCCCGCCCCGGTGCAG
                                                                              

HsCD00596604      ------------------------------------------------------------
NM_018026.3       ATGAACCTGTACGCCACCTGGGAGGTGGACCGGAGCTCGTCCAGCTGCGTGCCTAGGCTA
                                                                              

HsCD00596604      ------------------------------------------------------------
NM_018026.3       TTCAGCTTGACCCTGAAGAAACTCGTCATGCTAAAAGAAATGGACAAAGATCTTAACTCA
                                                                              

HsCD00596604      ------------------------------------------------------------
NM_018026.3       GTGGTCATCGCTGTGAAGCTGCAGGGTTCAAAAAGAATTCTTCGCTCCAACGAGATCGTC
                                                                              

HsCD00596604      ------------------------------------------------------------
NM_018026.3       CTTCCAGCTAGTGGACTGGTGGAAACAGAGCTCCAATTAACCTTCTCCCTTCAGTACCCT
                                                                              

HsCD00596604      ------------------------------------------------------------
NM_018026.3       CATTTCCTTAAGCGAGATGCCAACAAGCTGCAGATCATGCTGCAAAGGAGAAAACGTTAC
                                                                              

HsCD00596604      ------------------------------------------------------------
NM_018026.3       AAGAATCGGACCATCTTGGGCTATAAGACCTTGGCCGTGGGACTCATCAACATGGCAGAG
                                                                              

HsCD00596604      ------------------------------------------------------------
NM_018026.3       GTGATGCAGCATCCTAATGAAGGCGCACTGGTGCTTGGCCTACACAGCAACGTGAAGGAT
                                                                              

HsCD00596604      ------------------------------------------------------------
NM_018026.3       GTCTCTGTGCCTGTGGCAGAAATAAAGATCTACTCCCTGTCCAGCCAACCCATTGACCAT
                                                                              

HsCD00596604      ------------------------------------------------------------
NM_018026.3       GAAGGAATCAAATCCAAGCTTTCTGATCGTTCTCCTGATATTGACAATTATTCTGAGGAA
                                                                              

HsCD00596604      ------------------------------------------------------------
NM_018026.3       GAGGAAGAGAGTTTCTCATCAGAACAGGAAGGCAGTGATGATCCATTGCATGGGCAGGAC
                                                                              

HsCD00596604      ------------------------------------------------------------
NM_018026.3       TTGTTCTACGAAGACGAAGATCTCCGGAAAGTGAAGAAGACCCGGAGGAAACTAACCTCA
                                                                              

HsCD00596604      ------------------------------------------------------------
NM_018026.3       ACCTCTGCCATCACAAGGCAACCTAACATCAAACAGAAGTTTGTGGCCCTCCTGAAGCGG
                                                                              

HsCD00596604      ------------------------------------------------------------
NM_018026.3       TTTAAAGTTTCAGATGAGGTGGGCTTTGGGCTGGAGCATGTGTCCCGCGAGCAGATCCGG
                                                                              

HsCD00596604      ------------------------------------------------------------
NM_018026.3       GAAGTGGAAGAGGACTTGGATGAATTGTATGACAGTCTGGAGATGTACAACCCCAGCGAC
                                                                              

HsCD00596604      ------------------------------------------------------------
NM_018026.3       AGTGGCCCTGAGATGGAGGAGACAGAAAGCATCCTCAGCACGCCAAAGCCCAAGCTCAAG
                                                                              

HsCD00596604      ------------------------------------------------------------
NM_018026.3       CCTTTCTTTGAGGGGATGTCGCAGTCCAGCTCCCAGACGGAGATTGGCAGCCTCAACAGC
                                                                              

HsCD00596604      ------------------------------------------------------------
NM_018026.3       AAAGGCAGCCTCGGAAAAGACACCACCAGCCCTATGGAATTGGCTGCTCTAGAAAAAATT
                                                                              

HsCD00596604      ------------------------------------------------------------
NM_018026.3       AAATCTACTTGGATTAAAAACCAAGATGACAGCTTGACTGAAACAGACACTCTGGAAATC
                                                                              

HsCD00596604      ------------------------------------------------------------
NM_018026.3       ACTGACCAGGACATGTTTGGAGATGCCAGCACGAGTCTGGTTGTGCCGGAGAAAGTCAAA
                                                                              

HsCD00596604      ------------------------------------------------------------
NM_018026.3       ACTCCCATGAAGTCCAGTAAAACGGATCTCCAGGGCTCTGCCTCCCCCAGCAAAGTGGAG
                                                                              

HsCD00596604      ------------------------------------------------------------
NM_018026.3       GGGGTGCACACACCCCGGCAGAAGAGGAGCACGCCCCTGAAGGAGCGGCAGCTCTCCAAG
                                                                              

HsCD00596604      -----------------------------------------CCATCACCATCACCATCAC
NM_018026.3       CCCCTAAGTGAGAGGACCAACAGTTCCGACAGCGAGCGCTCCCCAGATCTGGGCCACAGC
                                                           **.: * *:  .*** ..*

HsCD00596604      AGCCATATGCCAAGAAAGGTGGTGTATGACCAGCTCAATCAGATCCTGGTGTCAGATGCA
NM_018026.3       ACGCAGATTCCAAGAAAGGTGGTGTATGACCAGCTCAATCAGATCCTGGTGTCAGATGCA
                  *  ** ** ***************************************************

HsCD00596604      GCCCTCCCAGAAAATGTCATTCTGGTGAACACCACTGACTGGCAGGGCCAGTATGTGGCT
NM_018026.3       GCCCTCCCAGAAAATGTCATTCTGGTGAACACCACTGACTGGCAGGGCCAGTATGTGGCT
                  ************************************************************

HsCD00596604      GAGCTGCTCCAGGACCAGCGGAAGCCTGTGGTGTGCACCTGCTCCACCGTGGAGGTCCAG
NM_018026.3       GAGCTGCTCCAGGACCAGCGGAAGCCTGTGGTGTGCACCTGCTCCACCGTGGAGGTCCAG
                  ************************************************************

HsCD00596604      GCCGTGCTGTCCGCCCTGCTCACCCGGATCCAGCGCTACTGCAACTGCAACTCTTCCATG
NM_018026.3       GCCGTGCTGTCCGCCCTGCTCACCCGGATCCAGCGCTACTGCAACTGCAACTCTTCCATG
                  ************************************************************

HsCD00596604      CCTAGGCCAGTGAAGGTGGCTGCTGTGGGAGGCCGGAGCTACCTGAGCTCCATCCTCAGG
NM_018026.3       CCGAGGCCAGTGAAGGTGGCTGCTGTGGGAGGCCAGAGCTACCTGAGCTCCATCCTCAGG
                  ** *******************************.*************************

HsCD00596604      TTCTTTGTCAAGTCCCTGGCCAACAAGACCTCCGACTGGCTTGGCTACATGCGCTTCCTC
NM_018026.3       TTCTTTGTCAAGTCCCTGGCCAACAAGACCTCCGACTGGCTTGGCTACATGCGCTTCCTC
                  ************************************************************

HsCD00596604      ATCATCCCCCTCGGTTCTCACCCTGAGGCCAAATACTTGGGGTCAGTCGACAGTAAATAC
NM_018026.3       ATCATCCCCCTCGGTTCTCACCCTGTGGCCAAATACTTGGGGTCAGTCGACAGTAAATAC
                  *************************:**********************************

HsCD00596604      AGTAGTTCCTTCCTGGATTCTGGTTGGAGAGATCTGTTCAGTCGCTCGGAGCCACCAGTG
NM_018026.3       AGTAGTTCCTTCCTGGATTCTGGTTGGAGAGATCTGTTCAGTCGCTCGGAGCCACCAGTG
                  ************************************************************

HsCD00596604      TCAGAGCAACTGGACGTGGCAGGGCGGGTGATGCAGTACGTCAACGGGGCAGCCACGACA
NM_018026.3       TCAGAGCAACTGGACGTGGCAGGGCGGGTGATGCAGTACGTCAACGGGGCAGCCACGACA
                  ************************************************************

HsCD00596604      CACCAGCTTCCCGTGGCCGAAGCCATGCTGACTTGCCGGCATAAGTTCCCTGATGAAGAC
NM_018026.3       CACCAGCTTCCCGTGGCCGAAGCCATGCTGACTTGCCGGCATAAGTTCCCTGATGAAGAC
                  ************************************************************

HsCD00596604      TCCTATCAGAAGTTTATTCCCTTCATTGGCGTGGTGAAGGTGGGTCTGGTTGAAGACTCT
NM_018026.3       TCCTATCAGAAGTTTATTCCCTTCATTGGCGTGGTGAAGGTGGGTCTGGTTGAAGACTCT
                  ************************************************************

HsCD00596604      CCCTCCACAGCAGGCGATGGGGACGATTCTCCTGTGGTCAGCCTTACTGTGCCCTCCACA
NM_018026.3       CCCTCCACAGCAGGCGATGGGGACGATTCTCCTGTGGTCAGCCTTACTGTGCCCTCCACA
                  ************************************************************

HsCD00596604      TCACCACCCTCCAGCTCGGGCCTGAGCCGAGACGCCACGGCCACCCCTCCCTCCTCCCCA
NM_018026.3       TCACCACCCTCCAGCTCGGGCCTGAGCCGAGACGCCACGGCCACCCCTCCCTCCTCCCCA
                  ************************************************************

HsCD00596604      TCTATGAGCAGCGCCCTGGCCATCGTGGGGAGCCCTAATAGCCCATATGGGGACGTGATT
NM_018026.3       TCTATGAGCAGCGCCCTGGCCATCGTGGGGAGCCCTAATAGCCCATATGGGGACGTGATT
                  ************************************************************

HsCD00596604      GGCCTCCAGGTGGACTACTGGCTGGGCCACCCCGGGGAGCGGAGGAGGGAAGGCGACAAG
NM_018026.3       GGCCTCCAGGTGGACTACTGGCTGGGCCACCCCGGGGAGCGGAGGAGGGAAGGCGACAAG
                  ************************************************************

HsCD00596604      AGGGACGCCAGCTCGAAGAACACCCTCAAGAGTGTCTTCCGCTCAGTGCAGGTGTCCCGC
NM_018026.3       AGGGACGCCAGCTCGAAGAACACCCTCAAGAGTGTCTTCCGCTCAGTGCAGGTGTCCCGC
                  ************************************************************

HsCD00596604      CTGCCCCATAGCGGGGAGGCCCAGCTTTCTGGCACCATGGCCATGACTGTGGTCACCAAA
NM_018026.3       CTGCCCCATAGTGGGGAGGCCCAGCTTTCTGGCACCATGGCCATGACTGTGGTCACCAAA
                  *********** ************************************************

HsCD00596604      GAAAAGAACAAGAAAGTTCCCACCATCTTCCTGAGCAAGAAACCCCGAGAAAAGGAGGTG
NM_018026.3       GAAAAGAACAAGAAAGTTCCCACCATCTTCCTGAGCAAGAAACCCCGAGAAAAGGAGGTG
                  ************************************************************

HsCD00596604      GATTCTAAGAGCCAGGTCATTGAAGGCATCAGCCGCCTCATCTGCTCAGCCAAGCAGCAG
NM_018026.3       GATTCTAAGAGCCAGGTCATTGAAGGCATCAGCCGCCTCATCTGCTCAGCCAAGCAGCAG
                  ************************************************************

HsCD00596604      CAGACTATGCTGAGAGTGTCCATCGATGGGGTCGAGTGGAGTGACATCCAGTTCTTCCAG
NM_018026.3       CAGACTATGCTGAGAGTGTCCATCGATGGGGTCGAGTGGAGTGACATCAAGTTCTTCCAG
                  ************************************************.***********

HsCD00596604      CTGGCAGCCCAGTGGCCCACCCATGTCAAGCACTTTCCAGTGGGACTCTTCAGTGGCAGC
NM_018026.3       CTGGCAGCCCAGTGGCCCACCCATGTCAAGCACTTTCCAGTGGGACTCTTCAGTGGCAGC
                  ************************************************************

HsCD00596604      AAGGCCACCTAG
NM_018026.3       AAGGCCACCTGA
                  **********..

Pathways

Reactome :

Disease

Reactome :

HIV Infection

Reactome :

Host Interactions of HIV factors

Reactome :

Nef mediated downregulation of MHC class I complex cell surface expression

Reactome :

Nef-mediates down modulation of cell surface receptors by recruiting them to clathrin adapters

Reactome :

The role of Nef in HIV-1 replication and disease pathogenesis

Protein

Structural Annotation Wiki :

HR3509D

UniProt :

Q6VY07

HPRD :

06320

Clone

Cloning Information :

629065

Original Clone ID :

HR3509D-544-963-15.3

Experimental

TargetTrack - Experimental data :

HR3509D

Organism

Species Specific ID:

55690

Genome

Chromosome :

Map Location :

11q13.1-q13.2

Ensembl :

ENSG00000175115

Reagents

Labome :

PACS1-antibody

PACS1 (H. sapiens )In pET15_NESG (His-tagged bacterial expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Pathways

Protein

Clone

Experimental

Organism

Genome

Reagents

PACS1 (H. sapiens )
In pET15_NESG (His-tagged bacterial expression vector)