CAD (H. sapiens )
In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  CAD
Gene Name:  carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase
Original Clone ID:
HR9133D-1918-2225-15.2
Keyword:
targeted domain
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pET15_NESG
Format: 
CLOSED
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
Rutgers University
PSI:Biology
Northeast Structural Genomics Consortium
HsCD00596833
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AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 930nts
Open reading frame: 51 to 980

CTTTAAGAAGGAGATATACCATGGGCCATCACCATCACCATCACAGCCATATGCTGCACT
CATTAGTGGGCCAACATATCCTGTCCGTCCAGCAGTTCACCAAGGATCAGATGTTTCACC
TGTTCAATGTGGCACACACACTGCGTATGATGGTGCAGAAGGAGCGGAGCCTCGACATCC
TGAAGGGGAAGGTCATGGCCTCCATGTTCTATGAAGTGAGCACACGGACCAGCAGCTCCT
TTGCAGCAGCCATGGCTCGGCTGGGAGGTGCTGTGCTCAGCTTCTCGGAAGCCACATCGT
CCGTCCAGAAGGGCGAATCCCTGGCTGACTCCGTGCAGACCATGAGCTGCTATGCCGACG
TCGTCATGCTCCGGCACCCCCAGCCTGGAGCAGTGGAGCTGGCCGCCAAGCACTGCCGGA
GGCCAGTGATCAATGCTGGGGGTGGGGTCGGAGAGCACCCCACCCAGGCCCTGCTGGACA
TCTTCACCATCCGTGAGGAGCTGGGAACTGTCAATGGCATGACGATCACGATGGTGGGTG
ACCTGAAGCACGGACGCACAGTACATTCCCTGGCCTGCCTGCTCACCCAGTATCGTGTCA
GCCTGCGCTACGTGGCACCTCCCAGCCTGCGCATGCCACCCACTGTGCGGGCCTTCGTGG
CCTCCCGCGGCACCAAGCAGGAGGAATTCGAGAGCATTGAGGAGGCGCTGCCTGACACTG
ATGTGCTCTACATGACTCGAATCCAGAAGGAACGATTTGGCTCTACCCAGGAGTACGAAG
CTTGCTTTGGTCAGTTCATCCTCACTCCCCACATCATGACCCGGACCAAGAAGAAGATGG
TGGTGATGCACCCGATGCCCCGTGTCAACGAGATAAGCGTGGAAGTGGACACGGATCCCC
GCGCAGCCTACTTCCGCCAGGCTGAGAACGGCATGTACATCCGCATGGCTCTGTTAGCCA
CCGTGCTGGGCCGTTTCTAGCTCGAGATCCGGCTG

Protein sequence predicted based on nucleotide sequence

MLHSLVGQHILSVQQFTKDQMFHLFNVAHTLRMMVQKERSLDILKGKVMASMFYEVSTRT
SSSFAAAMARLGGAVLSFSEATSSVQKGESLADSVQTMSCYADVVMLRHPQPGAVELAAK
HCRRPVINAGGGVGEHPTQALLDIFTIREELGTVNGMTITMVGDLKHGRTVHSLACLLTQ
YRVSLRYVAPPSLRMPPTVRAFVASRGTKQEEFESIEEALPDTDVLYMTRIQKERFGSTQ
EYEACFGQFILTPHIMTRTKKKMVVMHPMPRVNEISVEVDTDPRAAYFRQAENGMYIRMA
LLATVLGRF*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
c65t,S22F;c207t,A69A;

g316a,V106M;a392g,D131G;

g775a,A259T;t841a,S281T
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in HR9133D
Recommended expression in:
Not Applicable