DNASU Plasmid | ORC2 (H. sapiens )<br>In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: ORC2

Gene Name: origin recognition complex, subunit 2

Original Clone ID:

HR3138-1-561-14.5

Keyword:

full length protein truncation

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pET15_NESG

Format:

CLOSED

Source:

Northeast Structural Genomics Consortium

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

Rutgers University
PSI:Biology
Northeast Structural Genomics Consortium

HsCD00597153

Price:

AVAILABLE

No restriction
Special MTA: PSI/NESG

Insert Sequence
Protein Sequence

Insert sequence: 1686nts

Open reading frame: 51 to 1736

Reference Sequence Alignment

TTTAAGAAGGAGATATACCATGGGGCCATCACCATCACCATCACAGCCATATGAGTAAAC
CAGAATTAAAGGAAGACAAGATGCTGGAGGTTCACTTTGTGGGAGATGATGATGTTCTTA
ATCACATTCTAGATAGAGAAGGAGGAGCTAAATTGAAGAAGGAGCGAGCGCAGCTTTTGG
TCAACCCCAAAAAAATAATAAAGAAGCCAGAATATGATTTGGAGGAAGATGACCAGGAGG
TCTTAAAAGATCAGAACTATGTGGAAATTATGGGAAGAGATGTTCAAGAATCATTGAAAA
ATGGCTCTGCTACAGGTGGTGGAAATAAAGTTTATTCTTTTCAGAATAGAAAACACTCTG
AAAAGATGGCTAAATTAGCTTCAGAACTAGCAAAAACACCACAAAAAAGTGTTTCATTCA
GTTTGAAGAATGATCCTGAGATTACGATAAACGTTCCTCAAAGTAGCAAGGGCCATTCTG
CTTCAGACAAGGTTCAACCGAAGAACAATGACAAAAGTGAATTTCTGTCAACAGCACCTC
GTAGTCTAAGAAAAAGATTAATAGTTCCAAGGTCTCATTCTGACAGTGAAAGCGAATATT
CTGCTTCCAACTCAGAGGATGATGAAGGGGTTGCACTGGAACATGAAGAGGACACTAATG
CAGTCATATTCAGCCAAAAGATTCAAGCTCGGAATAGAATAGTTTCAGCTCCTGTTGGCA
AAGAAACACCTTCTAAGAGAATGAAAAGAGATAAAACAAGTGGCTTAGTAGAAGAATATT
TTGAAGCTCACAGCAGTTCAAAAGTTTTAACCTCTGATAGAACACTGCAGAAGCTAAAGA
GAGCTAAACTGGATCAGCAAACTTTGCGTAACTTATTGAGCAAGGTTTCCCCTTCCTTTT
CTGCCGAACTTAAACAACTAAATCAACAGTATGAAAAATTATTTCATAAATGGATGCTGC
AATTACACCTTGGGTTCAACATTGTGCTTTATGGTTTGGGTTCTAAGAGAGATTTACTAG
AAAGGTTTCGAACCACTATGCTGCAAGATTCCATTCACGTTGTCATCAATGGCTTCTTTC
CTGGAATCAGTGTGAAATCAGTCCTGAATTCTATAACAGAAGAAGTCCTCGATCATATGG
GTACTTTCCGCAGTATACTGGATCAGCTAGACCGGATAGTAAACAAATTTAAAGAAGATT
CTTCTTTAGAACTCTTCCTTCTCATCCACAATTTGGATAGCCAGATGTTGAGAGGAGAGA
AGAGCCAGCAAATCATTGGTCAGTTGTCATCTTTGCATAACATTTACCTTATAGCATCCA
TTGACCACCTCAATGCTCCTCTCATGTGGGATCATGCAAAGCAGAGTCTTTTTAACTGGC
TCTGGTATGAAACTACTACATACAGTCCTTATACTGAAGAAACCTCCTATGAGAACTCTC
TTCTGGTAAAGCAGTCTGGATCCCTGCCACTTAGCTCCCTTACTCATGTCTTACGAAGCC
TTACCCCTAACGCAAGGGGAATTTTCAGGCTACTAATAAAATACCAGCTGGACAACCAGG
ATAACCCTTCTTACATTGGCCTTTCTTTTCAAGATTTTTACCAGCAGTGTCGGGAGGCAA
TCCTCGTCAATAGTGATCTGACACTCCGGGCCCAGTTAACTGAATTTAGGGACCACAAGC
TTATAAGAACAAAGAAGGGAACTGATGGAGTAGAGTATTTATTAATTCCTGTTTAG

Protein sequence predicted based on nucleotide sequence

MSKPELKEDKMLEVHFVGDDDVLNHILDREGGAKLKKERAQLLVNPKKIIKKPEYDLEED
DQEVLKDQNYVEIMGRDVQESLKNGSATGGGNKVYSFQNRKHSEKMAKLASELAKTPQKS
VSFSLKNDPEITINVPQSSKGHSASDKVQPKNNDKSEFLSTAPRSLRKRLIVPRSHSDSE
SEYSASNSEDDEGVALEHEEDTNAVIFSQKIQARNRIVSAPVGKETPSKRMKRDKTSGLV
EEYFEAHSSSKVLTSDRTLQKLKRAKLDQQTLRNLLSKVSPSFSAELKQLNQQYEKLFHK
WMLQLHLGFNIVLYGLGSKRDLLERFRTTMLQDSIHVVINGFFPGISVKSVLNSITEEVL
DHMGTFRSILDQLDRIVNKFKEDSSLELFLLIHNLDSQMLRGEKSQQIIGQLSSLHNIYL
IASIDHLNAPLMWDHAKQSLFNWLWYETTTYSPYTEETSYENSLLVKQSGSLPLSSLTHV
LRSLTPNARGIFRLLIKYQLDNQDNPSYIGLSFQDFYQQCREAILVNSDLTLRAQLTEFR
DHKLIRTKKGTDGVEYLLIPV*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

a587t,Q196L;a641g,Q214R;

g649a,V217I;a713g,D238G;

t1123c,W375R;t1461c,N487N;

t1570a,F524I

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinPurified: Not_Tested

SolubleProtein: Not_Tested

ProteinExpressed: Tested_Not_Found

Find experimental protocols in HR3138

Recommended expression in:

Not Applicable

Vector Name:

pET15_NESG

Synonyms:

pET15, pET15-modified, pET15Cc, pET15_NESG

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.

Comments:

Modified Novagen pET vector-common MCS

Size (bp):

5723

Parent Vector:

None

Properties:

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker

Author Name:

Gaetano T. Montleione
Jessica Bearden
Northeast Structural Genomics Consortium

Publications:

PMID: 15808222

Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:

Vector Sequence:

Sequence:

TTCTCATGTTTGACAGCTTATCATCGATAAGCTAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCGAGAAGCTTTGCGTCGACAGAGCTCGCAGGATCCGCAGAATTCGCCATATGGCTGTGATGGTGATGGTGATGGCCCATGGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAA

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI)

327

452

bacterial origin

ori

ColE1 origin of replication

3881

4563

gene fragment

ROP

repressor of primer ROP sequence

3281

3457

primer

T7 forward

T7 forward sequencing primer

481

496

primer

T7 reverse

T7 reverse sequencing primer

257

275

promoter

T7 promoter

478

497

repressor binding site

lacO

lacO regulatory sequence

456

478

selectable marker

AmpR

ampicillin resistance gene

4661

5230

tag

His

N-terminal 6x His tag

387

404

trxn termination sequence

T7 term

T7 termination sequence

257

275

Gene

Gene Symbol :

ORC2

Symbol Nomenclature :

ORC2

SYNONYM :

ORC2L

Designation :

origin recognition complex protein 2 homolog|origin recognition complex subunit 2|origin recognition complex, subunit 2 homolog

Full Nomenclature :

origin recognition complex, subunit 2

GENEID :

4999

GenBank Accession :

NR_033915

HGNC :

8488

MIM :

601182

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00597153      TTTAAGAAGGAGATATACCATGGGGCCATCACCATCACCATCACAGCCATATGAGTAAAC
NM_006190.4       --------------------------------------------------ATGAGTAAAC
                                                                    **********

HsCD00597153      CAGAATTAAAGGAAGACAAGATGCTGGAGGTTCACTTTGTGGGAGATGATGATGTTCTTA
NM_006190.4       CAGAATTAAAGGAAGACAAGATGCTGGAGGTTCACTTTGTGGGAGATGATGATGTTCTTA
                  ************************************************************

HsCD00597153      ATCACATTCTAGATAGAGAAGGAGGAGCTAAATTGAAGAAGGAGCGAGCGCAGCTTTTGG
NM_006190.4       ATCACATTCTAGATAGAGAAGGAGGAGCTAAATTGAAGAAGGAGCGAGCGCAGCTTTTGG
                  ************************************************************

HsCD00597153      TCAACCCCAAAAAAATAATAAAGAAGCCAGAATATGATTTGGAGGAAGATGACCAGGAGG
NM_006190.4       TCAACCCCAAAAAAATAATAAAGAAGCCAGAATATGATTTGGAGGAAGATGACCAGGAGG
                  ************************************************************

HsCD00597153      TCTTAAAAGATCAGAACTATGTGGAAATTATGGGAAGAGATGTTCAAGAATCATTGAAAA
NM_006190.4       TCTTAAAAGATCAGAACTATGTGGAAATTATGGGAAGAGATGTTCAAGAATCATTGAAAA
                  ************************************************************

HsCD00597153      ATGGCTCTGCTACAGGTGGTGGAAATAAAGTTTATTCTTTTCAGAATAGAAAACACTCTG
NM_006190.4       ATGGCTCTGCTACAGGTGGTGGAAATAAAGTTTATTCTTTTCAGAATAGAAAACACTCTG
                  ************************************************************

HsCD00597153      AAAAGATGGCTAAATTAGCTTCAGAACTAGCAAAAACACCACAAAAAAGTGTTTCATTCA
NM_006190.4       AAAAGATGGCTAAATTAGCTTCAGAACTAGCAAAAACACCACAAAAAAGTGTTTCATTCA
                  ************************************************************

HsCD00597153      GTTTGAAGAATGATCCTGAGATTACGATAAACGTTCCTCAAAGTAGCAAGGGCCATTCTG
NM_006190.4       GTTTGAAGAATGATCCTGAGATTACGATAAACGTTCCTCAAAGTAGCAAGGGCCATTCTG
                  ************************************************************

HsCD00597153      CTTCAGACAAGGTTCAACCGAAGAACAATGACAAAAGTGAATTTCTGTCAACAGCACCTC
NM_006190.4       CTTCAGACAAGGTTCAACCGAAGAACAATGACAAAAGTGAATTTCTGTCAACAGCACCTC
                  ************************************************************

HsCD00597153      GTAGTCTAAGAAAAAGATTAATAGTTCCAAGGTCTCATTCTGACAGTGAAAGCGAATATT
NM_006190.4       GTAGTCTAAGAAAAAGATTAATAGTTCCAAGGTCTCATTCTGACAGTGAAAGCGAATATT
                  ************************************************************

HsCD00597153      CTGCTTCCAACTCAGAGGATGATGAAGGGGTTGCACTGGAACATGAAGAGGACACTAATG
NM_006190.4       CTGCTTCCAACTCAGAGGATGATGAAGGGGTTGCACAGGAACATGAAGAGGACACTAATG
                  ************************************:***********************

HsCD00597153      CAGTCATATTCAGCCAAAAGATTCAAGCTCGGAATAGAATAGTTTCAGCTCCTGTTGGCA
NM_006190.4       CAGTCATATTCAGCCAAAAGATTCAAGCTCAGAATAGAGTAGTTTCAGCTCCTGTTGGCA
                  ******************************.*******.*********************

HsCD00597153      AAGAAACACCTTCTAAGAGAATGAAAAGAGATAAAACAAGTGGCTTAGTAGAAGAATATT
NM_006190.4       AAGAAACACCTTCTAAGAGAATGAAAAGAGATAAAACAAGTGACTTAGTAGAAGAATATT
                  ******************************************.*****************

HsCD00597153      TTGAAGCTCACAGCAGTTCAAAAGTTTTAACCTCTGATAGAACACTGCAGAAGCTAAAGA
NM_006190.4       TTGAAGCTCACAGCAGTTCAAAAGTTTTAACCTCTGATAGAACACTGCAGAAGCTAAAGA
                  ************************************************************

HsCD00597153      GAGCTAAACTGGATCAGCAAACTTTGCGTAACTTATTGAGCAAGGTTTCCCCTTCCTTTT
NM_006190.4       GAGCTAAACTGGATCAGCAAACTTTGCGTAACTTATTGAGCAAGGTTTCCCCTTCCTTTT
                  ************************************************************

HsCD00597153      CTGCCGAACTTAAACAACTAAATCAACAGTATGAAAAATTATTTCATAAATGGATGCTGC
NM_006190.4       CTGCCGAACTTAAACAACTAAATCAACAGTATGAAAAATTATTTCATAAATGGATGCTGC
                  ************************************************************

HsCD00597153      AATTACACCTTGGGTTCAACATTGTGCTTTATGGTTTGGGTTCTAAGAGAGATTTACTAG
NM_006190.4       AATTACACCTTGGGTTCAACATTGTGCTTTATGGTTTGGGTTCTAAGAGAGATTTACTAG
                  ************************************************************

HsCD00597153      AAAGGTTTCGAACCACTATGCTGCAAGATTCCATTCACGTTGTCATCAATGGCTTCTTTC
NM_006190.4       AAAGGTTTCGAACCACTATGCTGCAAGATTCCATTCACGTTGTCATCAATGGCTTCTTTC
                  ************************************************************

HsCD00597153      CTGGAATCAGTGTGAAATCAGTCCTGAATTCTATAACAGAAGAAGTCCTCGATCATATGG
NM_006190.4       CTGGAATCAGTGTGAAATCAGTCCTGAATTCTATAACAGAAGAAGTCCTCGATCATATGG
                  ************************************************************

HsCD00597153      GTACTTTCCGCAGTATACTGGATCAGCTAGACCGGATAGTAAACAAATTTAAAGAAGATT
NM_006190.4       GTACTTTCCGCAGTATACTGGATCAGCTAGACTGGATAGTAAACAAATTTAAAGAAGATT
                  ******************************** ***************************

HsCD00597153      CTTCTTTAGAACTCTTCCTTCTCATCCACAATTTGGATAGCCAGATGTTGAGAGGAGAGA
NM_006190.4       CTTCTTTAGAACTCTTCCTTCTCATCCACAATTTGGATAGCCAGATGTTGAGAGGAGAGA
                  ************************************************************

HsCD00597153      AGAGCCAGCAAATCATTGGTCAGTTGTCATCTTTGCATAACATTTACCTTATAGCATCCA
NM_006190.4       AGAGCCAGCAAATCATTGGTCAGTTGTCATCTTTGCATAACATTTACCTTATAGCATCCA
                  ************************************************************

HsCD00597153      TTGACCACCTCAATGCTCCTCTCATGTGGGATCATGCAAAGCAGAGTCTTTTTAACTGGC
NM_006190.4       TTGACCACCTCAATGCTCCTCTCATGTGGGATCATGCAAAGCAGAGTCTTTTTAACTGGC
                  ************************************************************

HsCD00597153      TCTGGTATGAAACTACTACATACAGTCCTTATACTGAAGAAACCTCCTATGAGAACTCTC
NM_006190.4       TCTGGTATGAAACTACTACATACAGTCCTTATACTGAAGAAACCTCCTATGAGAACTCTC
                  ************************************************************

HsCD00597153      TTCTGGTAAAGCAGTCTGGATCCCTGCCACTTAGCTCCCTTACTCATGTCTTACGAAGCC
NM_006190.4       TTCTGGTAAAGCAGTCTGGATCCCTGCCACTTAGCTCCCTTACTCATGTCTTACGAAGCC
                  ************************************************************

HsCD00597153      TTACCCCTAACGCAAGGGGAATTTTCAGGCTACTAATAAAATACCAGCTGGACAACCAGG
NM_006190.4       TTACCCCTAATGCAAGGGGAATTTTCAGGCTACTAATAAAATACCAGCTGGACAACCAGG
                  ********** *************************************************

HsCD00597153      ATAACCCTTCTTACATTGGCCTTTCTTTTCAAGATTTTTACCAGCAGTGTCGGGAGGCAA
NM_006190.4       ATAACCCTTCTTACATTGGCCTTTCTTTTCAAGATTTTTACCAGCAGTGTCGGGAGGCAT
                  ***********************************************************:

HsCD00597153      TCCTCGTCAATAGTGATCTGACACTCCGGGCCCAGTTAACTGAATTTAGGGACCACAAGC
NM_006190.4       TCCTCGTCAATAGTGATCTGACACTCCGGGCCCAGTTAACTGAATTTAGGGACCACAAGC
                  ************************************************************

HsCD00597153      TTATAAGAACAAAGAAGGGAACTGATGGAGTAGAGTATTTATTAATTCCTGTTTAG----
NM_006190.4       TTATAAGAACAAAGAAGGGAACTGATGGAGTAGAGTATTTATTAATTCCTGTTGATAATG
                  ***************************************************** *     

HsCD00597153      --------------------------------------------
NM_006190.4       GAACATTGACTGATTTCTTGGAAAAGGAAGAAGAGGAGGCTTGA

Pathways

Reactome :

Activation of ATR in response to replication stress

Reactome :

Activation of the pre-replicative complex

Reactome :

Assembly of the ORC complex at the origin of replication

Reactome :

Assembly of the pre-replicative complex

Reactome :

Association of licensing factors with the pre-replicative complex

Reactome :

CDC6 association with the ORC:origin complex

Reactome :

CDT1 association with the CDC6:ORC:origin complex

Reactome :

Cell Cycle

Reactome :

Cell Cycle Checkpoints

Reactome :

Cell Cycle, Mitotic

Reactome :

DNA Replication

Reactome :

DNA Replication Pre-Initiation

Reactome :

E2F mediated regulation of DNA replication

Reactome :

E2F-enabled inhibition of pre-replication complex formation

Reactome :

G1/S Transition

Reactome :

G2/M Checkpoints

Reactome :

M/G1 Transition

Reactome :

Mitotic G1-G1/S phases

Reactome :

Mitotic M-M/G1 phases

Reactome :

Orc1 removal from chromatin

Reactome :

Regulation of DNA replication

Reactome :

Removal of licensing factors from origins

Reactome :

S Phase

Reactome :

Switching of origins to a post-replicative state

Reactome :

Synthesis of DNA

WikiPathway :

Cell cycle

WikiPathway :

DNA Replication

WikiPathway :

G1 to S cell cycle control

Protein

Structural Annotation Wiki :

HR3138

UniProt :

Q13416

HPRD :

03112

Clone

Cloning Information :

627283

Original Clone ID :

HR3138-1-561-14.5

Experimental

TargetTrack - Experimental data :

HR3138

Organism

Species Specific ID:

4999

Genome

Chromosome :

Map Location :

2q33

Reagents

Labome :

ORC2-antibody

ORC2 (H. sapiens )In pET15_NESG (His-tagged bacterial expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Pathways

Protein

Clone

Experimental

Organism

Genome

Reagents

ORC2 (H. sapiens )
In pET15_NESG (His-tagged bacterial expression vector)