DNASU Plasmid | CCNA1 (H. sapiens )<br>In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: CCNA1

Gene Name: cyclin A1

Original Clone ID:

HR2982-52-465-15.19

Keyword:

full length protein truncation

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pET15_NESG

Format:

CLOSED

Source:

Northeast Structural Genomics Consortium

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Rutgers University
PSI:Biology
Northeast Structural Genomics Consortium

HsCD00598056

Price:

AVAILABLE

No restriction
Special MTA: PSI/NESG

Insert Sequence
Protein Sequence

Insert sequence: 1248nts

Open reading frame: 15 to 1262

Reference Sequence Alignment

ACCATCACAGCCATATGAGTGGAGTTGTGCTGGCTACAGTGGCCCGAGGTCCCGATGCTT
GTCAGATACTCACCAGAGCCCCGCTGGGCCAGGATCCCCCGCAGAGGACAGTGCTAGGGC
TGCTAACTGCAAATGGGCAGTACAGGAGGACCTGTGGCCAGGGGATCACAAGAATCAGGT
GTTATTCTGGATCAGAAAATGCCTTCCCTCCAGCTGGAAAGAAAGCACTCCCTGACTGTG
GGGTCCAAGAGCCCCCCAAGCAAGGGTTTGACATCTACATGGATGAACTAGAGCAGGGGG
ACAGAGACAGCTGCTCGGTCAGAGAGGGGATGGCATTTGAGGATGTGTATGAAGTAGACA
CCGGCACACTCAAGTCAGACCTGCACTTCCTGCTGGATTTCAACACAGTTTCCCCTATGC
TGGTAGATTCATCTCTCCTCTCCCAGTCTGAAGATATATCCAGTCTTGGCACAGATGTGA
TAAATGTGACTGAATATGCTGAAGAAATTTATCAGTACCTTAGGGAAGCTGAAATAAGGC
ACAGACCCAAAGCACACTACATGAAGAAGCAGCCAGACATCACGGAAGGCATGCGCACGA
TTCTGGTGGACTGGCTGGTGGAGGTTGGGGAAGAATATAAACTTCGAGCAGAGACCCTGT
ATCTGGCTGTCAACTTCCTGGACAGGTTCCTTTCATGTATGTCTGTTCTGAGAGGGAAAC
TGCAGCTCGTAGGAACAGCAGCTATGCTTTTGGCTTCGAAATATGAAGAGATATATCCTC
CTGAAGTAGACGAGTTTGTCTATATCACCGATGATACATACACAAAACGACAACTGTTAA
AAATGGAACACTTGCTTCTGAAAGTTCTAGCTTTTGATCTGACAGTACCAACCACCAACC
AGTTTCTCCTTCAGTACTTGAGGCGACAAGGAGTGTGCGTCAGGACTGAGAACCTGGCTA
AGTACGTAGCAGAGCTGAGTCTACTTGAAGCAGATCCATTCTTGAAATATCTTCCTTCAC
TGATAGCTGCAGCAGCTTTTTGCCTGGCAAACTATACTGTGAACAAGCACTTTTGGCCAG
AAACCCTTGCTGCATTTACAGGGTATTCATTAAGTGAAATTGTGCCTTGCCTGAGTGAGC
TTCATAAAGCGTACCTTGATATACCCCATCGACCTCAGCAAGCAATTAGGGAGAAGTACA
AGGCTTCAAAGTACCTGTGTGTGTCCCTCATGGAGCCACCTGCAGTTCTTCTTCTACAAT
AG

Protein sequence predicted based on nucleotide sequence

MSGVVLATVARGPDACQILTRAPLGQDPPQRTVLGLLTANGQYRRTCGQGITRIRCYSGS
ENAFPPAGKKALPDCGVQEPPKQGFDIYMDELEQGDRDSCSVREGMAFEDVYEVDTGTLK
SDLHFLLDFNTVSPMLVDSSLLSQSEDISSLGTDVINVTEYAEEIYQYLREAEIRHRPKA
HYMKKQPDITEGMRTILVDWLVEVGEEYKLRAETLYLAVNFLDRFLSCMSVLRGKLQLVG
TAAMLLASKYEEIYPPEVDEFVYITDDTYTKRQLLKMEHLLLKVLAFDLTVPTTNQFLLQ
YLRRQGVCVRTENLAKYVAELSLLEADPFLKYLPSLIAAAAFCLANYTVNKHFWPETLAA
FTGYSLSEIVPCLSELHKAYLDIPHRPQQAIREKYKASKYLCVSLMEPPAVLLLQ*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinPurified: Not_Tested

SolubleProtein: Not_Tested

ProteinExpressed: Tested_Not_Found

Find experimental protocols in HR2982

Recommended expression in:

Not Applicable

Vector Name:

pET15_NESG

Synonyms:

pET15, pET15-modified, pET15Cc, pET15_NESG

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.

Comments:

Modified Novagen pET vector-common MCS

Size (bp):

5723

Parent Vector:

None

Properties:

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker

Author Name:

Gaetano T. Montleione
Jessica Bearden
Northeast Structural Genomics Consortium

Publications:

PMID: 15808222

Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:

Vector Sequence:

Sequence:

TTCTCATGTTTGACAGCTTATCATCGATAAGCTAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCGAGAAGCTTTGCGTCGACAGAGCTCGCAGGATCCGCAGAATTCGCCATATGGCTGTGATGGTGATGGTGATGGCCCATGGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAA

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI)

327

452

bacterial origin

ori

ColE1 origin of replication

3881

4563

gene fragment

ROP

repressor of primer ROP sequence

3281

3457

primer

T7 forward

T7 forward sequencing primer

481

496

primer

T7 reverse

T7 reverse sequencing primer

257

275

promoter

T7 promoter

478

497

repressor binding site

lacO

lacO regulatory sequence

456

478

selectable marker

AmpR

ampicillin resistance gene

4661

5230

tag

His

N-terminal 6x His tag

387

404

trxn termination sequence

T7 term

T7 termination sequence

257

275

Gene

Gene Symbol :

CCNA1

Symbol Nomenclature :

CCNA1

SYNONYM :

CT146

Designation :

cyclin-A1

Full Nomenclature :

cyclin A1

GENEID :

8900

GI :

4502611

GenBank Accession :

NM_003914

HGNC :

1577

MIM :

604036

Vega :

OTTHUMG00000016733

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00598056        ------------------------------------------------------------
NM_001111046.1      ------------------------------------------------------------
NM_001111047.1      ------------------------------------------------------------
NM_001111045.1      ATGGAGACCGGCTTTCCCGCAATCATGTACCCTGGATCTTTTATTGGGGGCTGGGGAGAA
NM_003914.3         ATGGAGACCGGCTTTCCCGCAATCATGTACCCTGGATCTTTTATTGGGGGCTGGGGAGAA
                                                                                

HsCD00598056        ------------------------------------------------------------
NM_001111046.1      ------------------------------------------------------------
NM_001111047.1      ------------------------------------------------------------
NM_001111045.1      GAGTATCTCAGCTGGGAAGGACCGGGGCTCCCAGATTTCGTCTTCCAGCAGCCCGTG---
NM_003914.3         GAGTATCTCAGCTGGGAAGGACCGGGGCTCCCAGATTTCGTCTTCCAGCAGCAGCCCGTG
                                                                                

HsCD00598056        ----------------ACCATCACAGCCATATGAGTGGAGTTGTGCTGGCTACAGTGGCC
NM_001111046.1      ------------ATGCACTGCAGCAACCCCAAGAGTGGAGTTGTGCTGGCTACAGTGGCC
NM_001111047.1      ------------ATGCACTGCAGCAACCCCAAGAGTGGAGTTGTGCTGGCTACAGTGGCC
NM_001111045.1      GAGTCTGAAGCAATGCACTGCAGCAACCCCAAGAGTGGAGTTGTGCTGGCTACAGTGGCC
NM_003914.3         GAGTCTGAAGCAATGCACTGCAGCAACCCCAAGAGTGGAGTTGTGCTGGCTACAGTGGCC
                                    ** . ..**.**. *:****************************

HsCD00598056        CGAGGTCCCGATGCTTGTCAGATACTCACCAGAGCCCCGCTGGGCCAGGATCCCCCGCAG
NM_001111046.1      CGAGGTCCCGATGCTTGTCAGATACTCACCAGAGCCCCGCTGGGCCAGGATCCCCCGCAG
NM_001111047.1      CGAGGTCCCGATGCTTGTCAGATACTCACCAGAGCCCCGCTGGGCCAGGATCCCCCGCAG
NM_001111045.1      CGAGGTCCCGATGCTTGTCAGATACTCACCAGAGCCCCGCTGGGCCAGGATCCCCCGCAG
NM_003914.3         CGAGGTCCCGATGCTTGTCAGATACTCACCAGAGCCCCGCTGGGCCAGGATCCCCCGCAG
                    ************************************************************

HsCD00598056        AGGACAGTGCTAGGGCTGCTAACTGCAAATGGGCAGTACAGGAGGACCTGTGGCCAGGGG
NM_001111046.1      AGGACAGTGCTAGGGCTGCTAACTGCAAATGGGCAGTACAGGAGGACCTGTGGCCAGGGG
NM_001111047.1      AGGACAGTGCTAGGGCTGCTAACTGCAAATGGGCAGTACAGGAGGACCTGTGGCCAGGGG
NM_001111045.1      AGGACAGTGCTAGGGCTGCTAACTGCAAATGGGCAGTACAGGAGGACCTGTGGCCAGGGG
NM_003914.3         AGGACAGTGCTAGGGCTGCTAACTGCAAATGGGCAGTACAGGAGGACCTGTGGCCAGGGG
                    ************************************************************

HsCD00598056        ATCACAAGAATCAGGTGTTATTCTGGATCAGAAAATGCCTTCCCTCCAGCTGGAAAGAAA
NM_001111046.1      ATCACAAGAATCAGGTGTTATTCTGGATCAGAAAATGCCTTCCCTCCAGCTGGAAAGAAA
NM_001111047.1      ATCACAAGAATCAGGTGTTATTCTGGATCAGAAAATGCCTTCCCTCCAGCTGGAAAGAAA
NM_001111045.1      ATCACAAGAATCAGGTGTTATTCTGGATCAGAAAATGCCTTCCCTCCAGCTGGAAAGAAA
NM_003914.3         ATCACAAGAATCAGGTGTTATTCTGGATCAGAAAATGCCTTCCCTCCAGCTGGAAAGAAA
                    ************************************************************

HsCD00598056        GCACTCCCTGACTGTGGGGTCCAAGAGCCCCCCAAGCAAGGGTTTGACATCTACATGGAT
NM_001111046.1      GCACTCCCTGACTGTGGGGTCCAAGAGCCCCCCAAGCAAGGGTTTGACATCTACATGGAT
NM_001111047.1      GCACTCCCTGACTGTGGGGTCCAAGAGCCCCCCAAGCAAGGGTTTGACATCTACATGGAT
NM_001111045.1      GCACTCCCTGACTGTGGGGTCCAAGAGCCCCCCAAGCAAGGGTTTGACATCTACATGGAT
NM_003914.3         GCACTCCCTGACTGTGGGGTCCAAGAGCCCCCCAAGCAAGGGTTTGACATCTACATGGAT
                    ************************************************************

HsCD00598056        GAACTAGAGCAGGGGGACAGAGACAGCTGCTCGGTCAGAGAGGGGATGGCATTTGAGGAT
NM_001111046.1      GAACTAGAGCAGGGGGACAGAGACAGCTGCTCGGTCAGAGAGGGGATGGCATTTGAGGAT
NM_001111047.1      GAACTAGAGCAGGGGGACAGAGACAGCTGCTCGGTCAGAGAGGGGATGGCATTTGAGGAT
NM_001111045.1      GAACTAGAGCAGGGGGACAGAGACAGCTGCTCGGTCAGAGAGGGGATGGCATTTGAGGAT
NM_003914.3         GAACTAGAGCAGGGGGACAGAGACAGCTGCTCGGTCAGAGAGGGGATGGCATTTGAGGAT
                    ************************************************************

HsCD00598056        GTGTATGAAGTAGACACCGGCACACTCAAGTCAGACCTGCACTTCCTGCTGGATTTCAAC
NM_001111046.1      GTGTATGAAGTAGACACCGGCACACTCAAGTCAGACCTGCACTTCCTGCTGGATTTCAAC
NM_001111047.1      GTGTATGAAGTAGACACCGGCACACTCAAGTCAGACCTGCACTTCCTGCTGGATTTCAAC
NM_001111045.1      GTGTATGAAGTAGACACCGGCACACTCAAGTCAGACCTGCACTTCCTGCTGGATTTCAAC
NM_003914.3         GTGTATGAAGTAGACACCGGCACACTCAAGTCAGACCTGCACTTCCTGCTGGATTTCAAC
                    ************************************************************

HsCD00598056        ACAGTTTCCCCTATGCTGGTAGATTCATCTCTCCTCTCCCAGTCTGAAGATATATCCAGT
NM_001111046.1      ACAGTTTCCCCTATGCTGGTAGATTCATCTCTCCTCTCCCAGTCTGAAGATATATCCAGT
NM_001111047.1      ACAGTTTCCCCTATGCTGGTAGATTCATCTCTCCTCTCCCAGTCTGAAGATATATCCAGT
NM_001111045.1      ACAGTTTCCCCTATGCTGGTAGATTCATCTCTCCTCTCCCAGTCTGAAGATATATCCAGT
NM_003914.3         ACAGTTTCCCCTATGCTGGTAGATTCATCTCTCCTCTCCCAGTCTGAAGATATATCCAGT
                    ************************************************************

HsCD00598056        CTTGGCACAGATGTGATAAATGTGACTGAATATGCTGAAGAAATTTATCAGTACCTTAGG
NM_001111046.1      CTTGGCACAGATGTGATAAATGTGACTGAATATGCTGAAGAAATTTATCAGTACCTTAGG
NM_001111047.1      CTTGGCACAGATGTGATAAATGTGACTGAATATGCTGAAGAAATTTATCAGTACCTTAGG
NM_001111045.1      CTTGGCACAGATGTGATAAATGTGACTGAATATGCTGAAGAAATTTATCAGTACCTTAGG
NM_003914.3         CTTGGCACAGATGTGATAAATGTGACTGAATATGCTGAAGAAATTTATCAGTACCTTAGG
                    ************************************************************

HsCD00598056        GAAGCTGAAATAAGGCACAGACCCAAAGCACACTACATGAAGAAGCAGCCAGACATCACG
NM_001111046.1      GAAGCTGAAATAAGGCACAGACCCAAAGCACACTACATGAAGAAGCAGCCAGACATCACG
NM_001111047.1      GAAGCTGAAATAAGGCACAGACCCAAAGCACACTACATGAAGAAGCAGCCAGACATCACG
NM_001111045.1      GAAGCTGAAATAAGGCACAGACCCAAAGCACACTACATGAAGAAGCAGCCAGACATCACG
NM_003914.3         GAAGCTGAAATAAGGCACAGACCCAAAGCACACTACATGAAGAAGCAGCCAGACATCACG
                    ************************************************************

HsCD00598056        GAAGGCATGCGCACGATTCTGGTGGACTGGCTGGTGGAGGTTGGGGAAGAATATAAACTT
NM_001111046.1      GAAGGCATGCGCACGATTCTGGTGGACTGGCTGGTGGAGGTTGGGGAAGAATATAAACTT
NM_001111047.1      GAAGGCATGCGCACGATTCTGGTGGACTGGCTGGTGGAGGTTGGGGAAGAATATAAACTT
NM_001111045.1      GAAGGCATGCGCACGATTCTGGTGGACTGGCTGGTGGAGGTTGGGGAAGAATATAAACTT
NM_003914.3         GAAGGCATGCGCACGATTCTGGTGGACTGGCTGGTGGAGGTTGGGGAAGAATATAAACTT
                    ************************************************************

HsCD00598056        CGAGCAGAGACCCTGTATCTGGCTGTCAACTTCCTGGACAGGTTCCTTTCATGTATGTCT
NM_001111046.1      CGAGCAGAGACCCTGTATCTGGCTGTCAACTTCCTGGACAGGTTCCTTTCATGTATGTCT
NM_001111047.1      CGAGCAGAGACCCTGTATCTGGCTGTCAACTTCCTGGACAGGTTCCTTTCATGTATGTCT
NM_001111045.1      CGAGCAGAGACCCTGTATCTGGCTGTCAACTTCCTGGACAGGTTCCTTTCATGTATGTCT
NM_003914.3         CGAGCAGAGACCCTGTATCTGGCTGTCAACTTCCTGGACAGGTTCCTTTCATGTATGTCT
                    ************************************************************

HsCD00598056        GTTCTGAGAGGGAAACTGCAGCTCGTAGGAACAGCAGCTATGCTTTTGGCTTCGAAATAT
NM_001111046.1      GTTCTGAGAGGGAAACTGCAGCTCGTAGGAACAGCAGCTATGCTTTTGGCTTCGAAATAT
NM_001111047.1      GTTCTGAGAGGGAAACTGCAGCTCGTAGGAACAGCAGCTATGCTTTTGGCTTCGAAATAT
NM_001111045.1      GTTCTGAGAGGGAAACTGCAGCTCGTAGGAACAGCAGCTATGCTTTTGGCTTCGAAATAT
NM_003914.3         GTTCTGAGAGGGAAACTGCAGCTCGTAGGAACAGCAGCTATGCTTTTGGCTTCGAAATAT
                    ************************************************************

HsCD00598056        GAAGAGATATATCCTCCTGAAGTAGACGAGTTTGTCTATATCACCGATGATACATACACA
NM_001111046.1      GAAGAGATATATCCTCCTGAAGTAGACGAGTTTGTCTATATCACCGATGATACATACACA
NM_001111047.1      GAAGAGATATATCCTCCTGAAGTAGACGAGTTTGTCTATATCACCGATGATACATACACA
NM_001111045.1      GAAGAGATATATCCTCCTGAAGTAGACGAGTTTGTCTATATCACCGATGATACATACACA
NM_003914.3         GAAGAGATATATCCTCCTGAAGTAGACGAGTTTGTCTATATCACCGATGATACATACACA
                    ************************************************************

HsCD00598056        AAACGACAACTGTTAAAAATGGAACACTTGCTTCTGAAAGTTCTAGCTTTTGATCTGACA
NM_001111046.1      AAACGACAACTGTTAAAAATGGAACACTTGCTTCTGAAAGTTCTAGCTTTTGATCTGACA
NM_001111047.1      AAACGACAACTGTTAAAAATGGAACACTTGCTTCTGAAAGTTCTAGCTTTTGATCTGACA
NM_001111045.1      AAACGACAACTGTTAAAAATGGAACACTTGCTTCTGAAAGTTCTAGCTTTTGATCTGACA
NM_003914.3         AAACGACAACTGTTAAAAATGGAACACTTGCTTCTGAAAGTTCTAGCTTTTGATCTGACA
                    ************************************************************

HsCD00598056        GTACCAACCACCAACCAGTTTCTCCTTCAGTACTTGAGGCGACAAGGAGTGTGCGTCAGG
NM_001111046.1      GTACCAACCACCAACCAGTTTCTCCTTCAGTACTTGAGGCGACAAGGAGTGTGCGTCAGG
NM_001111047.1      GTACCAACCACCAACCAGTTTCTCCTTCAGTACTTGAGGCGACAAGGAGTGTGCGTCAGG
NM_001111045.1      GTACCAACCACCAACCAGTTTCTCCTTCAGTACTTGAGGCGACAAGGAGTGTGCGTCAGG
NM_003914.3         GTACCAACCACCAACCAGTTTCTCCTTCAGTACTTGAGGCGACAAGGAGTGTGCGTCAGG
                    ************************************************************

HsCD00598056        ACTGAGAACCTGGCTAAGTACGTAGCAGAGCTGAGTCTACTTGAAGCAGATCCATTCTTG
NM_001111046.1      ACTGAGAACCTGGCTAAGTACGTAGCAGAGCTGAGTCTACTTGAAGCAGATCCATTCTTG
NM_001111047.1      ACTGAGAACCTGGCTAAGTACGTAGCAGAGCTGAGTCTACTTGAAGCAGATCCATTCTTG
NM_001111045.1      ACTGAGAACCTGGCTAAGTACGTAGCAGAGCTGAGTCTACTTGAAGCAGATCCATTCTTG
NM_003914.3         ACTGAGAACCTGGCTAAGTACGTAGCAGAGCTGAGTCTACTTGAAGCAGATCCATTCTTG
                    ************************************************************

HsCD00598056        AAATATCTTCCTTCACTGATAGCTGCAGCAGCTTTTTGCCTGGCAAACTATACTGTGAAC
NM_001111046.1      AAATATCTTCCTTCACTGATAGCTGCAGCAGCTTTTTGCCTGGCAAACTATACTGTGAAC
NM_001111047.1      AAATATCTTCCTTCACTGATAGCTGCAGCAGCTTTTTGCCTGGCAAACTATACTGTGAAC
NM_001111045.1      AAATATCTTCCTTCACTGATAGCTGCAGCAGCTTTTTGCCTGGCAAACTATACTGTGAAC
NM_003914.3         AAATATCTTCCTTCACTGATAGCTGCAGCAGCTTTTTGCCTGGCAAACTATACTGTGAAC
                    ************************************************************

HsCD00598056        AAGCACTTTTGGCCAGAAACCCTTGCTGCATTTACAGGGTATTCATTAAGTGAAATTGTG
NM_001111046.1      AAGCACTTTTGGCCAGAAACCCTTGCTGCATTTACAGGGTATTCATTAAGTGAAATTGTG
NM_001111047.1      AAGCACTTTTGGCCAGAAACCCTTGCTGCATTTACAGGGTATTCATTAAGTGAAATTGTG
NM_001111045.1      AAGCACTTTTGGCCAGAAACCCTTGCTGCATTTACAGGGTATTCATTAAGTGAAATTGTG
NM_003914.3         AAGCACTTTTGGCCAGAAACCCTTGCTGCATTTACAGGGTATTCATTAAGTGAAATTGTG
                    ************************************************************

HsCD00598056        CCTTGCCTGAGTGAGCTTCATAAAGCGTACCTTGATATACCCCATCGACCTCAGCAAGCA
NM_001111046.1      CCTTGCCTGAGTGAGCTTCATAAAGCGTACCTTGATATACCCCATCGACCTCAGCAAGCA
NM_001111047.1      CCTTGCCTGAGTGAGCTTCATAAAGCGTACCTTGATATACCCCATCGACCTCAGCAAGCA
NM_001111045.1      CCTTGCCTGAGTGAGCTTCATAAAGCGTACCTTGATATACCCCATCGACCTCAGCAAGCA
NM_003914.3         CCTTGCCTGAGTGAGCTTCATAAAGCGTACCTTGATATACCCCATCGACCTCAGCAAGCA
                    ************************************************************

HsCD00598056        ATTAGGGAGAAGTACAAGGCTTCAAAGTACCTGTGTGTGTCCCTCATGGAGCCACCTGCA
NM_001111046.1      ATTAGGGAGAAGTACAAGGCTTCAAAGTACCTGTGTGTGTCCCTCATGGAGCCACCTGCA
NM_001111047.1      ATTAGGGAGAAGTACAAGGCTTCAAAGTACCTGTGTGTGTCCCTCATGGAGCCACCTGCA
NM_001111045.1      ATTAGGGAGAAGTACAAGGCTTCAAAGTACCTGTGTGTGTCCCTCATGGAGCCACCTGCA
NM_003914.3         ATTAGGGAGAAGTACAAGGCTTCAAAGTACCTGTGTGTGTCCCTCATGGAGCCACCTGCA
                    ************************************************************

HsCD00598056        GTTCTTCTTCTACAATAG
NM_001111046.1      GTTCTTCTTCTACAATAA
NM_001111047.1      GTTCTTCTTCTACAATAA
NM_001111045.1      GTTCTTCTTCTACAATAA
NM_003914.3         GTTCTTCTTCTACAATAA
                    *****************.

Pathways

NCI :

C-MYB transcription factor network

Panther :

p53 pathway feedback loops 2

Reactome :

APC/C-mediated degradation of cell cycle proteins

Reactome :

Cdc20:Phospho-APC/C mediated degradation of Cyclin A

Reactome :

Cell Cycle

Reactome :

Cell Cycle, Mitotic

Reactome :

Cellular responses to stress

Reactome :

Cellular Senescence

Reactome :

Cyclin A/B1 associated events during G2/M transition

Reactome :

Cyclin A:Cdk2-associated events at S phase entry

Reactome :

Cyclin E associated events during G1/S transition

Reactome :

DNA Damage/Telomere Stress Induced Senescence

Reactome :

DNA Replication

Reactome :

E2F mediated regulation of DNA replication

Reactome :

G0 and Early G1

Reactome :

G1/S Transition

Reactome :

G1/S-Specific Transcription

Reactome :

G2 Phase

Reactome :

G2/M Transition

Reactome :

Mitotic G1-G1/S phases

Reactome :

Mitotic G2-G2/M phases

Reactome :

Orc1 removal from chromatin

Reactome :

Phosphorylation of proteins involved in the G2/M transition by Cyclin A:Cdc2 complexes

Reactome :

Regulation of APC/C activators between G1/S and early anaphase

Reactome :

Regulation of DNA replication

Reactome :

Regulation of mitotic cell cycle

Reactome :

Removal of licensing factors from origins

Reactome :

S Phase

Reactome :

SCF(Skp2)-mediated degradation of p27/p21

Reactome :

Senescence-Associated Secretory Phenotype (SASP)

Reactome :

Switching of origins to a post-replicative state

Reactome :

Synthesis of DNA

WikiPathway :

AMPK signaling

WikiPathway :

Cell cycle

WikiPathway :

G1 to S cell cycle control

Protein

Structural Annotation Wiki :

HR2982

UniProt :

P78396

HPRD :

04946

Clone

Cloning Information :

626137

Original Clone ID :

HR2982-52-465-15.19

Experimental

TargetTrack - Experimental data :

HR2982

Organism

Species Specific ID:

8900

Genome

Chromosome :

Map Location :

13q12.3-q13

Ensembl :

ENSG00000133101

Reagents

Labome :

CCNA1-antibody

CCNA1 (H. sapiens )In pET15_NESG (His-tagged bacterial expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Pathways

Protein

Clone

Experimental

Organism

Genome

Reagents

CCNA1 (H. sapiens )
In pET15_NESG (His-tagged bacterial expression vector)