Bacterial expression vector with T7 promoter and C-terminal TEV-cleavable 10xHis tag and TVMV-cleavable MBP tag; contains argU and ileX rare tRNA; ampicillin resistance in bacteria; ligation independent cloning
Comments:
None
Publications:
PMID:24057978 Title: New LIC vectors for production of proteins from genes containing rare codons
Authors:
Midwest Center for Structural Genomics
PSI:Biology
Midwest Center for Structural Genomics
Bacterial expression vector with T7 promoter and C-terminal TEV-cleavable 10xHis tag and TVMV-cleavable MBP tag; contains argU and ileX rare tRNA; ampicillin resistance in bacteria; ligation independent cloning
Comments:
LIC region plus a portion of the beta-lactamase gene from pMCSG29 removed by digestion with BglI and XbaI then ligated into pMCSG53 digested with the same enzymes. Product is pMCSG29 with truncated ori and the argU & ileX tRNA genes