Mammalian expression vector for expressing miRNAs; also contains red fluorescent protein, gateway cassette located within an intron of RFP 3'UTR, for ncRNA expression; ampicillin resistance in bacteria; Gateway cloning with L2R3 Gateway Cassette
Comments:
None
Publications:
PMID:25074381 Title: 3pLIFE: a functional assay to detect miRNA targets in high-throughput PMID:26645212 Title: A human 3_UTR clone collection to study post-transcriptional gene regulation
Authors:
Arizona State University
Marco Mangone
Justin Wolter
Center for Personalized Diagnostics
Mammalian expression vector for expressing miRNAs; also contains red fluorescent protein, gateway cassette located within an intron of RFP 3'UTR, for ncRNA expression; ampicillin resistance in bacteria; Gateway cloning with L2R3 Gateway Cassette
Comments:
Growth before gateway recombination requires DB3.1 E. coli cells.