Detailed Vector Information: pLIFE-miRNA

Explanation of Terms

Gene:
Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID:
None
Type:
No insert
Vector Name:
pLIFE-miRNA
Source:
Mangone Laboratory
Description:
Mammalian expression vector for expressing miRNAs; also contains red fluorescent protein, gateway cassette located within an intron of RFP 3'UTR, for ncRNA expression; ampicillin resistance in bacteria; Gateway cloning with L2R3 Gateway Cassette
Comments:
None
Publications:
PMID: 25074381
Title: 3pLIFE: a functional assay to detect miRNA targets in high-throughput
PMID: 26645212
Title: A human 3_UTR clone collection to study post-transcriptional gene regulation
Authors:
Arizona State University
Marco Mangone
Justin Wolter
Center for Personalized Diagnostics
EvNO00601504
Price: 
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AVAILABLE
No restriction
Special MTA: SPTA

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.