Detailed Plasmid Information

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH236849.01F
Keyword:
None
Species:
Vibrio cholerae O1 biovar El Tor str. N16961
Type:
cDNA
Vector Name:
pDONR221
Format: 
FUSION
Source:
HIP
Description:
None
Comments:
Reference contains "authentic frameshift" but this clone does not have that frameshift.
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HIP
VcCD00061261
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3255nts
Open reading frame: 1 to 3255

ATGCATTTGCCGCATTTTGCTCTGAGCTCAGTGCTCTCGCTAACGTTGTGTGCGATACCG
AATCTTGGTTTTGCAAGCTCCAACCAGACTGAAGATTCCACGCAGGCTCGGCCGACTTGG
TTGAGGGATATTGCGTTGTCCCCCGATGGTCAAAAGATAGCGTTTACTTATGCAGGTCAA
ATTTGGCTGGTGCCGGCTCAAGGCGGTGATGCCGTGGCGCTCACCGAAAGCGGTGTGTAC
AGCGAAACGCCGATTTGGTCACCTGACAGCCAATCGATCGCTTTTACGGCTGATCGATAT
GGTCTTGGTGATGTTTTTATTCTCTCGATCCAAGGTGGTGAGAGTCGGCGCTTGACCTAC
CACGGAGCGAAAGATATTCCGTATGCGTTTTCGGCTGATGGTCAGCAGCTCTATTTCTCT
TCTCGCCGACTGGGCGATGATAAAGCCAATGCGAACGTTAAACAGGGCAGCTTTATGGCT
CAGCTTTACTCGGTGCCTGCAGCGGGAGGACGTGAGCAACGCGTTTTACCTATTGCCATC
AGTGATTTAGCCATATCGCCTTCCCATAGTGACATCCTGTATACCAATCAACCCTCTGAT
GAACAGCCGTGGCGTAAAGGTGCGCTATCCGATGCTACACGTGACATTTGGCAATGGTCC
CCGCTCACGGGCAAACATACCCAAATCACCACTTTTCGTGGCGAAGACCGCAACCCTGTG
TGGAGTGCAGATGGCTCCTCTATGTACTATCTGTCTGAACAAGCGGGCAGTTTCAACGTC
TGGCAGCAGCGATTTGATGGTTCAGAACCCGTACAAATCACCGACCACCAAAAGCTGCCT
GTTCGTTTCTTAAGTGCCAGCTTACAAGGCGATTTGGCCTACGGTTTTGATGGCGAAATT
TGGCGACTGAAAGCCGGAGCTAAGCAAGCAGAAAAAGTGCCGGTCTCCATTCGTCGCAGC
GCGATGCCTGATGGCCGTCATAATGTGAATTTTAACCTTGAAGCCACGGAAATGGTGGTC
GCGCCGAATGCCGCTGAAGTGGCGATTGTGGCACGTGGCGATGTGTATGTGGTCTCTTTG
CTTTCCGGTTTAACGCAACGAATCACCGATACACCAGAAGCGGAGCGCGATGTTTCCTTT
TCCAGTGATGGGTATCGTTTGATCTACGCTTCTGAGCGTGAGGGCAGTTGGAATCTTTAT
CAAAGTTATGTGAATGATGGTGGGAAAAGCTTCTCCTCCTCATTGGATATTATCGAAGAG
CCGGTGCTGACGACGGAGCAAGATGTCATTCAGCCACTCTATTCTCCTAATCTTAAACGG
ATTGTTTATCGCGAGAATCGTAATACGCTCAAAGTGTATGACATCGAACAAGATAAAACC
TATACCTTGCTGGATGCTCATGCCCTCTACTCGTATTTCGACAAAGATCTGAGTTACCAG
TGGTCGCCAGATAGTGAGTATATCGTCACTCGCGATCGGGCGATGTCGAATGGCGACATT
CAGCTACTTAAATTTGATGGAAGTGAAGCGCCTATCAATTTGAGCCAAAGTGGTTTTTCT
GAGTTTGCCCCACAATTTAGTGCCGATGGACAGTGGGTTTACTGGCTAACCGATGCGAAA
GGTTTGCGCGATATTGATGATATGGTGGTGCAGTATGATGTGTACGGTGTTGCGCTCAAT
CGCGAAGCCAAATTCAATTTCAATAAAACCCAAGAGCAGTTGTGGCTTGAAGAGGAAATC
GCTGCGGAGAAAAACCTTGGGCCTGGCCAAAACCCACCAGCAGAGTTAACGGTGGTGGAA
AATAAAGGGCTTAAGCAGCGCACTATGCGTATGACTCCTACCTCACTCAATATTATTTTC
AAGCACTTAACGCACGATAACCAAGCACTGATCATCGCTTATCAGTTGGGAGACTCAGTA
CAAATTTCAGAAATCAATCTGCGCAGTGGGGAGATGACGGCGCTGTTTAACCGCTTGAGT
GAAGATGCGGCTTTACTTGCGATGGCTTCTGATGATGCGAGCCTTCTAATCATGGGGGAG
CACGGTATCGAGAATCTGAATGTCTTGACGGGTGAGAGCAAGTTTGTTCGTTATGAAGCC
AAGGCTAATTTCGATTTTCGTGCAGAAATCGCCTACTTGTTTGATCATGTCTGGCGACTT
ACTCAAACCAAATTTTATGACCCGCAAATGCATGGTGTAGATTGGCAACAGTACGGTGAT
TTGTATCGTAAACATCTGCCGAGCATCCGTACCTACAGCGATTTTGCCGAGTTACTGAGT
GAAATGGTCGGGGATTTGAATGTCTCCCATACAGGAGCCTTCTTTATGGCTGGTAACTCC
AGTTGGGAGGAGCCCGCATCCTTAGGGCTTTACTATGATGATCGTTACCGAGGTAAGGGC
GTGCGTGTGAAATCACTGCTACCCGGAGGCCCTGCAGATACTTATCAATCTCCGATCAAG
GCAGGAGCGATTATTTACTCCGTAAACGGGAAAGAAATTAGCGATCAACAGGATATTTAT
CCGTTCCTGAATTTTACCCAAGGTAAATTAACTCGTTTAAGTGTGCTGGTACCGGGGGAG
GAGAAAGCGCAGAACTTTACGTTAGTGCCGATCACTCTTGAAGAGGAAAGTGAGCTGCTT
TATGAGCAGTGGGTTGAACAGCGCCGAGCCTTAGTGGAGACGCTCTCCGATGGACGCCTT
GGCTATGTGCATCTTGCCGCGATGGATGCTGCCAGTTTTGAGCAGATGCAAAATGACATG
TTTGGCCTTGAAAAAGACAAACTCGGCTTAGTGGTCGACGTGCGTTTTAATGCTGGAGGT
TGGCTGCATGATCAAGTGATGGAGATATTGTCTGGAACGCGACATTCAGTCATGCAGACA
CGTGATGGTTATGTAGTCTCCTCTTTCCCTGAACGTCGCTGGGCGAAACCGAGCATTATG
CTCGCCAACGCAGACAGTTATTCAGACGGTTCGATCGTACCGTATTTCTATCAAAAAGAA
GGGCTAGGAAAGCTAGTGGGCGAAAGGGTTCCCGGTACTGGCACGGCAGTGATTTGGGAG
CAGCAACAAGAGCCCGGATTGATTTACGGAGTGCCGCAACTCGGTATCAAGGATGAGCAA
GGTCGTTGGTTTGAAAACCAAGAAATCATCCCAGATATTTTGGTTTATAACGATCCGGAA
TCGGTTGTCGCCGGCGAGGATCGCCAGTTGGCTGCCGCCGTAGAAGCCCTGCTGCTCGAG
ATCTCTTCAAAATTG

Protein sequence predicted based on nucleotide sequence

MHLPHFALSSVLSLTLCAIPNLGFASSNQTEDSTQARPTWLRDIALSPDGQKIAFTYAGQ
IWLVPAQGGDAVALTESGVYSETPIWSPDSQSIAFTADRYGLGDVFILSIQGGESRRLTY
HGAKDIPYAFSADGQQLYFSSRRLGDDKANANVKQGSFMAQLYSVPAAGGREQRVLPIAI
SDLAISPSHSDILYTNQPSDEQPWRKGALSDATRDIWQWSPLTGKHTQITTFRGEDRNPV
WSADGSSMYYLSEQAGSFNVWQQRFDGSEPVQITDHQKLPVRFLSASLQGDLAYGFDGEI
WRLKAGAKQAEKVPVSIRRSAMPDGRHNVNFNLEATEMVVAPNAAEVAIVARGDVYVVSL
LSGLTQRITDTPEAERDVSFSSDGYRLIYASEREGSWNLYQSYVNDGGKSFSSSLDIIEE
PVLTTEQDVIQPLYSPNLKRIVYRENRNTLKVYDIEQDKTYTLLDAHALYSYFDKDLSYQ
WSPDSEYIVTRDRAMSNGDIQLLKFDGSEAPINLSQSGFSEFAPQFSADGQWVYWLTDAK
GLRDIDDMVVQYDVYGVALNREAKFNFNKTQEQLWLEEEIAAEKNLGPGQNPPAELTVVE
NKGLKQRTMRMTPTSLNIIFKHLTHDNQALIIAYQLGDSVQISEINLRSGEMTALFNRLS
EDAALLAMASDDASLLIMGEHGIENLNVLTGESKFVRYEAKANFDFRAEIAYLFDHVWRL
TQTKFYDPQMHGVDWQQYGDLYRKHLPSIRTYSDFAELLSEMVGDLNVSHTGAFFMAGNS
SWEEPASLGLYYDDRYRGKGVRVKSLLPGGPADTYQSPIKAGAIIYSVNGKEISDQQDIY
PFLNFTQGKLTRLSVLVPGEEKAQNFTLVPITLEEESELLYEQWVEQRRALVETLSDGRL
GYVHLAAMDAASFEQMQNDMFGLEKDKLGLVVDVRFNAGGWLHDQVMEILSGTRHSVMQT
RDGYVVSSFPERRWAKPSIMLANADSYSDGSIVPYFYQKEGLGKLVGERVPGTGTAVIWE
QQQEPGLIYGVPQLGIKDEQGRWFENQEIIPDILVYNDPESVVAGEDRQLAAAVEALLLE
ISSKL

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 3255
Mutations:
None
Discrepancy:
del@2129,1
                                                    Reference contains "authentic frameshift" but this clone does not have that frameshift.

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable