hrpA (V. cholerae O1 biovar El Tor str. N16961 )
In pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH236886.01F
Keyword:
None
Species:
Vibrio cholerae O1 biovar El Tor str. N16961
Type:
cDNA
Vector Name:
pDONR221
Format: 
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
VcCD00061276
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3930nts
Open reading frame: 1 to 3930

ATGACTTCGTCACAGCCCACACCCGATTCGGCAGCTCAGCCAAAAGCCAATAGCGCTGCC
AGTTTAAAAAAGGCGCTCGGAGAGTGCTTGATCAAAGACCGTTTTCGTTTCAGTAAACGC
ATTGATGGTGCCAGTAAAATTAAAAATGAATCGGCACGCAACGCGGTATTTGACGAAATC
GCGCTCGATATTGCGCAATCCATGATGGTGGTTGAGCAGCGTAAACAGCAGATGCCCAAA
ATCGAGTATCCTGCGCTGCTGCCAGTTAGCCAAAAACGCGATGATATTGCGCAGGCGATT
GCTCATCATCAAGTGGTGATCGTGGCCGGTGAAACCGGTTCAGGCAAAACCACTCAGCTA
CCGAAAATTTGTGCAGAATTAGGCCGTGGTAAATACGGTTTGATTGGCCACACTCAGCCG
CGCCGTCTTGCTGCGCGATCAGTGGCAAACCGCATTGCCGAAGAGATGGAAACCGAACTC
GGTGGCTTTGTGGGCTATAAAGTTCGCTTTACCGATCAAATCTCGGATCAGACTCAAATC
AAATTGATGACTGACGGTATCTTGCTGGCGGAGATCCAAAACGATCGTTTCCTCAACCAG
TACGACACCATCATTATCGATGAAGCGCACGAGCGTAGCCTCAACATCGACTTTATCTTA
GGCTATCTCAAGCAGCTTTTGCCGCGTCGTCCCGATCTCAAAGTCATCATCACCTCGGCG
ACCATCGACCCTGAGCGCTTCTCGAAGCATTTTAGCAATGCGCCGATCATCGAAGTCTCA
GGCCGTACTTATCCTGTGGAAGTGCGCTATCGCCCTCTGGCGGGGGATGATGACAGCGAA
AGCGATCGCGACCAACTGGAAGGCATTTTCCAAGCCGTGGATGAGCTGTGCGATGAAGGG
CTGGGCGACATTCTGATCTTCATGAACGGTGAGCGCGAAATTCGTGATACCGCGGATGCG
CTTTCAAAGCGCAACCTGCGTGATACGGAAATTGTGCCACTGTATGCACGTTTGTCAGCC
GGTGAGCAGAACAAGATTTTCCAACCTCATGCGGGTCGTCGCATTGTGCTGGCCACCAAC
GTGGCGGAAACCTCGCTAACGGTTCCGGGCATCAAATACGTTATCGACCCAGGTACCGCG
CGGATCAGTCGTTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCC
CAAGCCAGCGCTAACCAGCGTAAAGGCCGCTGTGGACGTACCGAAGAGGGGATCTGTATC
CGTCTCTATTCGGAAGAGGATTTCTTGTCACGCCCTGAGTTTACTGACCCTGAGATTCTG
CGCACCAACTTAGCCTCGGTTATCTTGCAGATGACGGCATTGGGGCTAGGCGATATTGAA
GCCTTCCCCTTTGTGGAAGCGCCAGATAAGCGCAACATTCAAGATGGTGTGCGTCTGCTC
GAAGAGCTAGGGGCTATCAACGATCAAATCAAAGACCCGAAAAAACGCCTCACTGAAAGT
GGTAAGCAGCTAGCGCGTTTGCCCATTGATCCACGTTTGGCACGCATGGTGCTCGAAGCG
TCTAAATTAGGCTGTTTGAAAGAAGTGATGATTATTGCTTCCGCGTTGTCGATTCAAGAT
CCGCGTGAAAGACCGAGCGACAAGCAGCAATCGGCCGATGACAAACATCGCCGCTTTAAT
CATGAAGATTCAGATTTCCTGACGTTAGTGAACTTATGGCACTACATCGGACAGCAGCAA
AAAGCGTTGACCAGTAACCAGTTCCGCCGTCAGTGCAAGCTTGATTACCTCAACTATTTG
CGTGTGCGCGAATGGCAGGATGTGTACACCCAACTGCATCAATCGACTCGGGAAATGGGT
TTTAAGCTCAATGATGAGCCGGGCAGTTACCATGCGGTGCACAGTGCGATCTTGGTCGGT
TTGTTGTCGCACATCGGTATGAAAGATCAGGAGAAGAACGAGTACCACGGCGCACGTAAC
GCACGTTTCAACATTTTTCCTGCCTCTGGCTTATTTAAAAAGCAGCCGAAATGGGTCATG
TCGGCAGAGCTGGTGGAAACCTCCAAACTGTGGGCGCGCGTGGTTGCGAAAATCGAACCG
GATTGGATTGAGCCACTAGCGAAACACCTGAGCAAGCGCAGTTACAGTGAGCCACATTGG
TCGAAGAAAAATGCGGCGGTGATGGCGTATGAAAAAGTGATGCTGTACGGCATCCCGATT
GTTCCCAAGCGCTTGGTCAACTATGGCACGATTGATCCTGTGCTCAGTCGCGAAATTTTC
ATTCGCAGCGCACTGGTAGAAGGGGATTGGGAAACCAAACACGCCTTCTTCAAACAAAAC
CGCGCGCTACTCGCCGAAGTGGAAGAGTTAGAACACAAATCGCGTCGCCGCGATATTCTG
GTTGATGATGAAGAGTTGTTCCAATTCTACGACCAGCGTGTCGGTACCGAAGTGGTGTCT
GGTCGCCATTTTGATGCGTGGTGGAAAACCGCTAGTCGTAAAACGCCGGATCTGCTCAGC
TTTGAAAAAGAGATGCTGTTCAAAGGCGATGCCAGCCACATTACCGATCTGGATTACCCG
AATTTCTGGCATCAAGGCAATCTGAAACTCAAACTCAGTTACCAGTTTGAGCCGGGCGAG
AACAGTGACGGTGTGACGGTACACATTCCGCTGCCAATCTTAAATCAAGTTGAGCCACAT
GGCTTTGACTGGCAGATTCCCGGTTTGCGCCATGAGTTGGTGGTGAGCTTAATTAAATCG
CTACCGAAAACGCTGCGTAAAAACTTTGTGCCAGCCCCCAATTACGCTGACGCTTTTTTG
GCGCGTGTAACACCGTTTGAAATGCCGCTGCTCGATGCAATGGAAAAAGAGCTGCGCCGC
ATGACGGGTGTGACGGTGCTGCGTGAAGATTGGAAACTCGATCAACTGCCTGCGCATTTA
AAAATTACTTATCGCGCGGTGGATCATCGCAATCGCAAGCTCAATGAAAGCTGCGATCTG
CATGAGCTGAAAGAGAGCTTGAAAGAGAAAGTGCAAGAGACACTTTCACAAGTGGCCGAT
GACGATATCGAGCAGCGCGATCTGCATACGTGGTCGTTTGGTGAGCTACCGAAAGTGTAC
CAGCAAAAGCGTGGTGGCTTTGAAGTTAGAGCTTATCCGGCGTTAGTCGATAAGAAAGAC
AGCGTCGAAATCAAACTGTTTGAAACCGAGCAAGAGCAGCTTAGCGCGATGCGTGCCGGT
CAGCGCCGTTTGATTTTGCTTAACGTGCCATCACCGATCAAATATCTGCACGCCAATCTG
CCGAACAAATCTAAACTCGGTTTGTACTTTAACCCGTATGGCAAAGTGCTCGATTTGATT
GATGACTGCATTGCCTGCGGCGTTGATAAGCTGATTGAAGAGCGCGGCGGCATGGTGTGG
GAGCCACAAGCATTTGAAGCGCTCAAAGAGCATGTGCGTGCCGAGCTTGGCGATACAGTA
GTTGAGATTGCCAAGCAGGTAGAAACCATTTTGACCACGGCTTACAACATCAATAAGCGT
TTGAAAGGTAAGGTGGATTTCACGATGGCGTTTGCGCTTTCTGATGTGAAAGCACAAATC
GAAGGGCTGATTTTCAGCGGCTTTGCGACTGAGTGCGGTTGGAAACGTCTGCCGGACATT
CTGCGTTACATGCGCGCGATTGAACGCCGGATGGAAAAACTGCCGGTCGATCCCAACAAA
GATCGTCTGCATATGCTGAAAATTGAATCGGTAGCCAACAAGTATAAAGAGCTGCTGAAC
AAGATCCCGAAAGGGATGGCGATTCCCGATAACGTGCGAGAAATCCGCTGGATGCTCGAA
GAGTTACGCGTGAGTTATTTTGCTCAGCAATTGGGTACGCCGTATCCGGTTTCGGATAAG
CGGATCATCAACGCGATTGAGGCTTGTTTG

Protein sequence predicted based on nucleotide sequence

MTSSQPTPDSAAQPKANSAASLKKALGECLIKDRFRFSKRIDGASKIKNESARNAVFDEI
ALDIAQSMMVVEQRKQQMPKIEYPALLPVSQKRDDIAQAIAHHQVVIVAGETGSGKTTQL
PKICAELGRGKYGLIGHTQPRRLAARSVANRIAEEMETELGGFVGYKVRFTDQISDQTQI
KLMTDGILLAEIQNDRFLNQYDTIIIDEAHERSLNIDFILGYLKQLLPRRPDLKVIITSA
TIDPERFSKHFSNAPIIEVSGRTYPVEVRYRPLAGDDDSESDRDQLEGIFQAVDELCDEG
LGDILIFMNGEREIRDTADALSKRNLRDTEIVPLYARLSAGEQNKIFQPHAGRRIVLATN
VAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQASANQRKGRCGRTEEGICI
RLYSEEDFLSRPEFTDPEILRTNLASVILQMTALGLGDIEAFPFVEAPDKRNIQDGVRLL
EELGAINDQIKDPKKRLTESGKQLARLPIDPRLARMVLEASKLGCLKEVMIIASALSIQD
PRERPSDKQQSADDKHRRFNHEDSDFLTLVNLWHYIGQQQKALTSNQFRRQCKLDYLNYL
RVREWQDVYTQLHQSTREMGFKLNDEPGSYHAVHSAILVGLLSHIGMKDQEKNEYHGARN
ARFNIFPASGLFKKQPKWVMSAELVETSKLWARVVAKIEPDWIEPLAKHLSKRSYSEPHW
SKKNAAVMAYEKVMLYGIPIVPKRLVNYGTIDPVLSREIFIRSALVEGDWETKHAFFKQN
RALLAEVEELEHKSRRRDILVDDEELFQFYDQRVGTEVVSGRHFDAWWKTASRKTPDLLS
FEKEMLFKGDASHITDLDYPNFWHQGNLKLKLSYQFEPGENSDGVTVHIPLPILNQVEPH
GFDWQIPGLRHELVVSLIKSLPKTLRKNFVPAPNYADAFLARVTPFEMPLLDAMEKELRR
MTGVTVLREDWKLDQLPAHLKITYRAVDHRNRKLNESCDLHELKESLKEKVQETLSQVAD
DDIEQRDLHTWSFGELPKVYQQKRGGFEVRAYPALVDKKDSVEIKLFETEQEQLSAMRAG
QRRLILLNVPSPIKYLHANLPNKSKLGLYFNPYGKVLDLIDDCIACGVDKLIEERGGMVW
EPQAFEALKEHVRAELGDTVVEIAKQVETILTTAYNINKRLKGKVDFTMAFALSDVKAQI
EGLIFSGFATECGWKRLPDILRYMRAIERRMEKLPVDPNKDRLHMLKIESVANKYKELLN
KIPKGMAIPDNVREIRWMLEELRVSYFAQQLGTPYPVSDKRIINAIEACL

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 3930
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable