Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
FLH220773.01F
Keyword:
None
Species:
Vibrio cholerae O1 biovar El Tor str. N16961
Type:
cDNA
Vector Name:
pANT7_cGST
Format:
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
VcCD00061296
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3996nts
Open reading frame: 1 to 3996
ATGCGCTCGATAAAAGCGATGTTCGTACTACTGTTTTGCTTGATGGCGATATTGGCGGTT
GCCATGTTTAAAGTGACTCGGCAAGTCGCAGATCTTAGGCTAGAGAGCCATCAATACAGC
GAGCAGCTTTTTCACTTTTATCGTCTATCTCAAGAGTTAAAACAAAGCTCAGACCACTTG
ACTAAGTTTGCCCGAGCGTATGCTAGCACTGGCAATGAGCGATGGTTGCGTATGTTTAAC
CAAGTTTTGAATGTCCGTAATGGCAAACTGCCAGCACCAAGCAATAATGATTACGAGTTT
TGGGATGTAGTTGTACTTAACGATTCGAGTGAACTTACACCAAACGCTAATCAGCGCTAT
CCCACATTGATTGAACGCATTCGTCAATCTGGTATTGGGTCGGTCGAGTTCCTTGAATTA
CAAAATGCGTTAGCGTTATCGGATCAGCTAGTCGCATTGGAGCGTGAAGCCTTCATGGCA
GTGAAGGGGTGGAAGAGAGAGTTTGCGGGGGAATACCGCTATACCGGGGAGCCAGATCTT
AACTTTGCTCGTTCTTTGCTTTATAGCGAAAAGTATTTTCTCGAGAAAGCCAAAATCATG
GCTGCAATTGGATCGGCGCACTCCAATATCGTAGATCGTATTGAATCTGAAGTGCGAGCG
GCTGATGAAAAAGCGCAATTTTATGAACGAGTTAATTTAGTTTTAGTCAGTTTATCACTG
TTGGTCATCATCGCTTCTTTCATACTACTTTGGGTTATTTATATTGCGCCATTGAGTATT
TTACTGAAAACGGTGGTGAGACAAGTTCGCAGTGAAGATTATAAATTTACCCTGACTCAA
AAAGCGTATGGCGAATTGCAACGCTTTATCGATAGTTTGAATGTGGTGTTTCACCATATT
TCCGAACAGCTCAGTTTTAATACCTTGGTGAAAGACTTCAACATCATCATTCGCAACCAT
CCGACCACGGAAGCCTTGTGCAATCAGGTGACACAGTTTTTACTGCACCATTTCCCCGTT
GAACTGATAGGTCTTTATCTCTATCAAAATGGTGTACTGACACGCATTGCCGGTGTTGGT
CAGTCGGAGTCTGCACAACGTGACTACAGTGATCCAACGACAACGCATTGTAGTGTGCTT
CGTTCGGGCAAGCCTTATGCAATGAAGTCACTCAATGGCAACTTCCAAGTGGTTTCTACC
TCAGGAGTGATTGCACTCAATGAGGTGTATTACCTACCTCTACAGGTCAATAATCAGCCG
ATAGCGTTACTGGAGTTAGGAACTGTAGGCATGCTGACAATGCAGCAATACCATTGGTTA
TCGCAGATGCTTGACGATCTCTCTGTCGCGATTCAACTTAGCCAAAATGCGGAGCAGCAA
CGTTTGGCAGAAAGAAAAGTGCTTGAACAATCACAGCTCAATCAAGAGATCCTCGATGCT
ACACCCAACCCGATGTACTGCTTGAGTGCAGATGGTAAATTCCTCACTGTCAATGCCAAG
TTCTTGGATCTCATGGGTTTATACAATAACGAAGTGATTGATAAAGAGCCGCAAGAGATT
TTCCCCGCTGAAGTTGCAGAGCGCTTTGCTGCGGCGCACCAAGTGTTGACTCAGCAACAA
AGTAGCCAAAATTACGAAGTTTCTTTGGTCGATTACACGGGTGTTCAGCGTGAAATGCTG
GTTTACGAGGCGTCTTTTAGCGATCGTGCGGACAAGGTCAATGGTATCGTCGGTATTTTG
TTGGATATGACCGAACGCAAAGAGATGGAAGATGAACTCCGCAAAGCCAAAGAGGCGGCG
GACGCGATGAGTCAGGCCAAAGGTGACTTCTTAGCGAATATGAGCCATGAAATCCGAACC
CCAATGAATGCAATTTTGGGTATGGCACATCTGGCATTGAATACCCCACTCGATACGACA
CAACGCAAATACGTGACTCGTATTAATGAATCCGCCAAAAACTTACTGGGCATTATCAAT
GACATTCTCGACTTTTCCAAAATGGAGGCGGGTAAACTCAATGTAGAACGGATCGATTTC
AATCTTGATGATGTGCTGGATAATGTGACGGCAGTCGTCTCATTGAAAGCCCAAGAGAAA
GGCATTGAGTTTTTGATGGATATCGACCCGCACATCCCACTAGGGTTGATTGGCGATCCG
CTTAGGCTTGGTCAAGTGATGGTCAATCTGTGTGGTAATGCGGTGAAGTTTACCTCACAA
GGCGAAATTGTACTGTCTGCACGAGTGAGCCAATTGAATGAACAGCAAGTCACGCTGCGG
TTTGCCGTCAAAGATACAGGGATTGGCATTGCCCAAGACAAGCTGGACGATTTGTTCGAT
GCTTTCTCGCAGGCAGATAACAGCATTACGCGTCAATATGGGGGCACTGGGCTTGGATTG
AGCATCAGTAAGCAATTGGTCGAGCTTATGGGAGGGCAAATTACCGTTCATAGTGAAGAA
GGTAAAGGTTCCACCTTTGAGTTTTCTGTGGTGTGTGGTCTGCAAGATGCCAAAATGCGT
GATATTTCCCAACCTGTGCATGGTCTAGCGGATAAGCGAGTGTTAGTGGTGGATGATAAT
GATTCTGCTCGCCACATTCTCGACTCTTTACTGACCGCTATGCGTTTTAACGTCGTGACG
GTGAGTAATGGGTTTGAGGCGCTATCGGAAATTCAACAGAAACCCTTTGATATGTTGTTT
GTTGACTGGAACATGCCTGGTATGAATGGCATTGAGCTACTGACGCGAGTGAAAACCCTC
GGCCTTCGTGGGCACATGAAGAGCTTTTTAGTGACGGCTTATGGACGTGAGATTAACCTT
GATGGCGAAAACAGCAAGTTGGTTGACGCTTTAATTGTCAAACCGGTTAATCCCTCGAAT
CTGCTGGATGCGATTGTGACCAGTTATGGAATTGAGCATGTACGCCATAAAACGAACGAA
GCGCATCACAACAGCAGGCCGAATTTTGCCGGCCAGAGTCTGTTGCTCGTCGAAGATAAT
GAGGTGAACCAAGAAGTTGCGCTTGGCCTACTCCAAGACACTGGCCTTAATGTCGTGGTC
GCCAATCATGGTCAAGAAGCGCTAGAGAGGTTAGAGCATGCCGATTTTGATCTGGTGCTA
ATGGATATGCAGATGCCGGTCATGGATGGGATCACCGCGACTGAGCATATTCGAGCCAAT
CCGCGGTGGCATAGCTTACCCATCGTTGCTATGACCGCTAATGCGATGGCGATAGATATT
GAACGCTGCTTGCAAGCAGGCATGAATGATCATCTTTCCAAACCGATTGAAGTGGATAAG
TTTTATCAGGTACTCAGGCATTTTCTTCAGGTGAGTGATGAGCAGAGCTTTTCATCGCAT
TCCCCAAATAAACCCAGCGCATCAAAACCTGCGATGAACGCTTTGTTGGATCTGCCTCAG
CTGACGGGCGTTGATTTGGAACAAGCTATCTTCCGGATTGGGGGCAATCAACAACGCTAT
TTTGAAATTCTGCGTCACTTTATCGATAGCCAAGTTGATGAGCTGAACAGTGTAACAGGG
CTAATCGAAGCGCAAGACTGGAGCTCTGCGACCCGAGTGGCTCATACTCTGAAAGGTTCT
GCCGCGAATTTAGGATTAGAAGCTTTAGCTCAGTTGGCGGCAGAGATGGAGCAGAGTCTA
GAGCAGCAAGTCCTACCTTCGTTTGAGCTGCTTCAACAAGCTCAAAGCTTGCTAACCCAA
GTCGATGATGAACTCTCTGCGTGGGAACATCAGCATAAAGAGCCGCTATTGGCGGAGAAC
CTAGATTGGGGTTTGTGGTATCAGCAGTTGCAACAGGCGATCAATGATTATGATGTCACC
GCATTAAGCCTGAGTAAGTCCATTCGCCAAGTTTCCCAGTGGGATTCGGCGCAGCAAGCC
GCCTTAATTTCTGCAATTGAAGATTTTGATTTTGAGCAGGCAAAGGCGCTATTAGCCAGT
TATCCGCAGTTAAGCGGTGAGCGTTTAAACCAGTTG
Protein sequence predicted based on nucleotide sequence
MRSIKAMFVLLFCLMAILAVAMFKVTRQVADLRLESHQYSEQLFHFYRLSQELKQSSDHL TKFARAYASTGNERWLRMFNQVLNVRNGKLPAPSNNDYEFWDVVVLNDSSELTPNANQRY PTLIERIRQSGIGSVEFLELQNALALSDQLVALEREAFMAVKGWKREFAGEYRYTGEPDL NFARSLLYSEKYFLEKAKIMAAIGSAHSNIVDRIESEVRAADEKAQFYERVNLVLVSLSL LVIIASFILLWVIYIAPLSILLKTVVRQVRSEDYKFTLTQKAYGELQRFIDSLNVVFHHI SEQLSFNTLVKDFNIIIRNHPTTEALCNQVTQFLLHHFPVELIGLYLYQNGVLTRIAGVG QSESAQRDYSDPTTTHCSVLRSGKPYAMKSLNGNFQVVSTSGVIALNEVYYLPLQVNNQP IALLELGTVGMLTMQQYHWLSQMLDDLSVAIQLSQNAEQQRLAERKVLEQSQLNQEILDA TPNPMYCLSADGKFLTVNAKFLDLMGLYNNEVIDKEPQEIFPAEVAERFAAAHQVLTQQQ SSQNYEVSLVDYTGVQREMLVYEASFSDRADKVNGIVGILLDMTERKEMEDELRKAKEAA DAMSQAKGDFLANMSHEIRTPMNAILGMAHLALNTPLDTTQRKYVTRINESAKNLLGIIN DILDFSKMEAGKLNVERIDFNLDDVLDNVTAVVSLKAQEKGIEFLMDIDPHIPLGLIGDP LRLGQVMVNLCGNAVKFTSQGEIVLSARVSQLNEQQVTLRFAVKDTGIGIAQDKLDDLFD AFSQADNSITRQYGGTGLGLSISKQLVELMGGQITVHSEEGKGSTFEFSVVCGLQDAKMR DISQPVHGLADKRVLVVDDNDSARHILDSLLTAMRFNVVTVSNGFEALSEIQQKPFDMLF VDWNMPGMNGIELLTRVKTLGLRGHMKSFLVTAYGREINLDGENSKLVDALIVKPVNPSN LLDAIVTSYGIEHVRHKTNEAHHNSRPNFAGQSLLLVEDNEVNQEVALGLLQDTGLNVVV ANHGQEALERLEHADFDLVLMDMQMPVMDGITATEHIRANPRWHSLPIVAMTANAMAIDI ERCLQAGMNDHLSKPIEVDKFYQVLRHFLQVSDEQSFSSHSPNKPSASKPAMNALLDLPQ LTGVDLEQAIFRIGGNQQRYFEILRHFIDSQVDELNSVTGLIEAQDWSSATRVAHTLKGS AANLGLEALAQLAAEMEQSLEQQVLPSFELLQQAQSLLTQVDDELSAWEHQHKEPLLAEN LDWGLWYQQLQQAINDYDVTALSLSKSIRQVSQWDSAQQAALISAIEDFDFEQAKALLAS YPQLSGERLNQL
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Insert was fully sequenced in parent vector
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
3996
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pANT7_cGST
Synonyms:
pANT7-cGST
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp):
5964
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
3931
3249
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1876
2085
primer
NAP138
NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?
2405
2384
primer
NAP150
Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?
388
408
promoter
T7
T7 transcriptional start sequence
5940
5964
recombination site
attR1
AttR1 site for recombination in empty vector
549
565
recombination site
attR2
AttR2 site for recombination in empty vector
2236
2251
selectable marker
CmR
chloramphenicol resistance
782
1438
selectable marker
AmpR
ampicillin resistance gene
4688
4029
tag
GST
Glutathione transferase (GST) tag
2282
2950
