Gene:
Gene Symbol:
WWP1
Gene Name:
WW domain containing E3 ubiquitin protein ligase 1
Original Clone ID:
HsCD00298953
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00616870
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2769nts
Open reading frame: 285 to 3053
GAGGGGCTGCTGGCGGCCTGGGCTGCCGGGGCCGACGCCTGGGTGGCTGCTGCCGCCGCG
CCTGCTGCGAGATGGCGATCTTGGGCGCGGAAGGGTGAGGGCGCCCGCCGCAGGAGGAGG
TGCCGCTGCCGTGGCCGCCCGGCTGCCGGGAGCCGACAGCTTCGCGCCGGGGTTGTCTCC
TCACAGACTATGAGCTCCTTGAAAGAGGGAATCGTGTCTTACTCATCTTTGTATCCCCAG
TGTCTAGCAGTTCCTGATACATAGTTTTAGCTGAATTTTGGGACATGGCCACTGCTTCAC
CAAGGTCTGATACTAGTAATAACCACAGTGGAAGGTTGCAGTTACAGGTAACTGTTTCTA
GTGCCAAACTTAAAAGAAAAAAGAACTGGTTCGGAACAGCAATATATACAGAAGTAGTTG
TAGATGGAGAAATTACGAAAACAGCAAAATCCAGTAGTTCTTCTAATCCAAAATGGGATG
AACAGCTAACTGTAAATGTTACGCCACAGACTACATTGGAATTTCAAGTTTGGAGCCATC
GCACTTTAAAAGCAGATGCTTTATTAGGAAAAGCAACGATAGATTTGAAACAAGCTCTGT
TGATACACAATAGAAAATTGGAAAGAGTGAAAGAACAATTAAAACTTTCCTTGGAAAACA
AGAATGGCATAGCACAAACTGGTGAATTGACAGTTGTGCTTGATGGATTGGTGATTGAGC
AAGAAAATATAACAAACTGCAGCTCATCTCCAACCATAGAAATACAGGAAAATGGTGATG
CCTTACATGAAAATGGAGAGCCTTCAGCAAGGACAACTGCCAGGTTGGCTGTTGAAGGCA
CGAATGGAATAGATAATCATGTACCTACAAGCACTCTAGTCCAAAACTCATGCTGCTCGT
ATGTAGTTAATGGAGACAACACACCTTCATCTCCGTCTCAGGTTGCTGCCAGACCCAAAA
ATACACCAGCTCCAAAACCACTCGCATCTGAGCCTGCCGATGACACTGTTAATGGAGAAT
CATCCTCATTTGCACCAACTGATAATGCGTCTGTCACGGGTACTCCAGTAGTGTCTGAAG
AAAATGCCTTGTCTCCAAATTGCACTAGTACTACTGTTGAAGATCCTCCAGTTCAAGAAA
TACTGACTTCCTCAGAAAACAATGAATGTATTCCTTCTACCAGTGCAGAATTGGAATCTG
AAGCTAGAAGTATATTAGAGCCTGACACCTCTAATTCTAGAAGTAGTTCTGCTTTTGAAG
CAGCCAAATCAAGACAGCCAGATGGGTGTATGGATCCTGTACGGCAGCAGTCTGGGAATG
CCAACACAGAAACCTTGCCATCAGGGTGGGAACAAAGAAAAGATCCTCATGGTAGAACCT
ATTATGTGGATCATAATACTCGAACTACCACATGGGAGAGACCACAACCTTTACCTCCAG
GTTGGGAAAGAAGAGTTGATGATCGTAGAAGAGTTTATTATGTGGATCATAACACCAGAA
CAACAACGTGGCAGCGGCCTACCATGGAATCTGTCCGAAATTTTGAACAGTGGCAATCTC
AGCGGAACCAATTGCAGGGAGCTATGCAACAGTTTAACCAACGATACCTCTATTCGGCTT
CAATGTTAGCTGCAGAAAATGACCCTTATGGACCTTTGCCACCAGGCTGGGAAAAAAGAG
TGGATTCAACAGACAGGGTTTACTTTGTGAATCATAACACAAAAACAACCCAGTGGGAAG
ATCCAAGAACTCAAGGCTTACAGAATGAAGAACCCCTGCCAGAAGGCTGGGAAATTAGAT
ATACTCGTGAAGGTGTAAGGTACTTTGTTGATCATAACACAAGAACAACAACATTCAAAG
ATCCTCGCAATGGGAAGTCATCTGTAACTAAAGGTGGTCCACAAATTGCTTATGAACGCG
GCTTTAGGTGGAAGCTTGCTCACTTCCGTTATTTGTGCCAGTCTAATGCACTACCTAGTC
ATGTAAAGATCAATGTGTCCCGGCAGACATTGTTTGAAGATTCCTTCCAACAGATTATGG
CATTAAAACCCTATGACTTGAGGAGGCGCTTATATGTAATATTTAGAGGAGAAGAAGGAC
TTGATTATGGTGGCCTAGCGAGAGAATGGTTTTTCTTGCTTTCACATGAAGTTTTGAACC
CAATGTATTGCTTATTTGAGTATGCGGGCAAGAACAACTATTGTCTGCAGATAAATCCAG
CATCAACCATTAATCCAGACCATCTTTCATACTTCTGTTTCATTGGTCGTTTTATTGCCA
TGGCACTATTTCATGGAAAGTTTATCGATACTGGTTTCTCTTTACCATTCTACAAGCGTA
TGTTAAGTAAAAAACTTACTATTAAGGATTTGGAATCTATTGATACTGAATTTTATAACT
CCCTTATCTGGATAAGAGATAACAACATTGAAGAATGTGGCTTAGAAATGTACTTTTCTG
TTGACATGGAGATTTTGGGAAAAGTTACTTCACATGACCTGAAGTTGGGAGGTTCCAATA
TTCTGGTGACTGAGGAGAACAAAGATGAATATATTGGTTTAATGACAGAATGGCGTTTTT
CTCGAGGAGTACAAGAACAGACCAAAGCTTTCCTTGATGGTTTTAATGAAGTTGTTCCTC
TTCAGTGGCTACAGTACTTCGATGAAAAAGAATTAGAGGTTATGTTGTGTGGCATGCAGG
AGGTTGACTTGGCAGATTGGCAGAGAAATACTGTTTATCGACATTATACAAGAAACAGCA
AGCAAATCATTTGGTTTTGGCAGTTTGTGAAAGAGACAGACAATGAAGTAAGAATGCGAC
TATTGCAGTTCGTCACTGGAACCTGCCGTTTACCTCTAGGAGGATTTGCTGAGCTCATGG
GAAGTAATGGGCCTCAAAAGTTTTGCATTGAAAAAGTTGGCAAAGACACTTGGTTACCAA
GAAGCCATACATGTTTTAATCGCTTGGATCTACCACCATATAAGAGTTATGAACAACTAA
AGGAAAAACTTCTTTTTGCAATAGAAGAGACAGAGGGATTTGGACAAGAATGAATGTGGC
TTCTTATTTTGGAGGAGCTCTTGCATTTAAATACCCCAGCCGAGAAAAATTGCACAGATA
GTGTATATAAGCTGTTCATTCTGTACAGTGAATTTTCCGAACCTCTCAAAGTATGTTTTC
CGTTCTTCCACAGAAATATGCAAAACAGTTCATCCTTTTCTACTTTATTTATTGTTCCCT
TGAAATGACTGACCAGGAAAAAGATCATCCTTAAATTTTGAAGCAAGTGAGAGACTTTAT
TAAAAATACATATATATCTATATAAACATATATGATAGTGGCTCTAGTTTTATAGAGCTC
CAAGTGTATTAAACATGACAGCCATTCATTCATAAAGATCTGGATTTGCTTTACCTTGTT
AATATTATCTAGGGGAAAAAGTGCAAATTGCTCCATGTTCTTCTCTCCCTTATGTAACAT
CTCCTGAGGGTGTTTAGTTGCATGGCTGTTCAGAAAGGTATTAAGGGCTTAGGCCAAATC
TTACTTTGAGTATGTTAAAAAAAAAAAAATGCTGCTGGCTTTTCTGAAGACAGGTGCTTG
AACTTGTCAGTTTGTTTTAAATAAATACAATAGTTGAAAATTTTTCTCTGTTACATCAGT
AATATTGTTAAAGTAATGGATAGAACCATAACTTACACATGAAAGTCATATACTAGATCC
AATACTATTTAGTTTATTATCGAAATTGGAAGGATTCATTGAGCAGCATAGAAGTTTGTT
TACATGTTACTTTGAGATGCTAGGTATTTGTGGAATTAAAAAGAATCAGGCTCTTTTGCA
AAAAAAAAAAAAAAA
Protein sequence predicted based on nucleotide sequence
MATASPRSDTSNNHSGRLQLQVTVSSAKLKRKKNWFGTAIYTEVVVDGEITKTAKSSSSS NPKWDEQLTVNVTPQTTLEFQVWSHRTLKADALLGKATIDLKQALLIHNRKLERVKEQLK LSLENKNGIAQTGELTVVLDGLVIEQENITNCSSSPTIEIQENGDALHENGEPSARTTAR LAVEGTNGIDNHVPTSTLVQNSCCSYVVNGDNTPSSPSQVAARPKNTPAPKPLASEPADD TVNGESSSFAPTDNASVTGTPVVSEENALSPNCTSTTVEDPPVQEILTSSENNECIPSTS AELESEARSILEPDTSNSRSSSAFEAAKSRQPDGCMDPVRQQSGNANTETLPSGWEQRKD PHGRTYYVDHNTRTTTWERPQPLPPGWERRVDDRRRVYYVDHNTRTTTWQRPTMESVRNF EQWQSQRNQLQGAMQQFNQRYLYSASMLAAENDPYGPLPPGWEKRVDSTDRVYFVNHNTK TTQWEDPRTQGLQNEEPLPEGWEIRYTREGVRYFVDHNTRTTTFKDPRNGKSSVTKGGPQ IAYERGFRWKLAHFRYLCQSNALPSHVKINVSRQTLFEDSFQQIMALKPYDLRRRLYVIF RGEEGLDYGGLAREWFFLLSHEVLNPMYCLFEYAGKNNYCLQINPASTINPDHLSYFCFI GRFIAMALFHGKFIDTGFSLPFYKRMLSKKLTIKDLESIDTEFYNSLIWIRDNNIEECGL EMYFSVDMEILGKVTSHDLKLGGSNILVTEENKDEYIGLMTEWRFSRGVQEQTKAFLDGF NEVVPLQWLQYFDEKELEVMLCGMQEVDLADWQRNTVYRHYTRNSKQIIWFWQFVKETDN EVRMRLLQFVTGTCRLPLGGFAELMGSNGPQKFCIEKVGKDTWLPRSHTCFNRLDLPPYK SYEQLKEKLLFAIEETEGFGQE*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: chloramphenicol
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
