PKLR (H. sapiens )
In pLDNT7_nFLAG (Flag-tagged
In vitro expression vector)

Explanation of Terms

Gene:
Gene Symbol:  PKLR
Gene Name:  pyruvate kinase, liver and RBC
Original Clone ID:
HsCD00299377
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format: 
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00617909
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1725nts
Open reading frame: 26 to 1750

GCAGCCCCAGGCCCACACTGAAAGCATGTCGATCCAGGAGAACATATCATCCCTGCAGCT
TCGGTCATGGGTCTCTAAGTCCCAAAGAGACTTAGCAAAGTCCATCCTGATTGGGGCTCC
AGGAGGGCCAGCGGGGTATCTGCGGCGGGCCAGTGTGGCCCAACTGACCCAGGAGCTGGG
CACTGCCTTCTTCCAGCAGCAGCAGCTGCCAGCTGCTATGGCAGACACCTTCCTGGAACA
CCTCTGCCTACTGGACATTGACTCCGAGCCCGTGGCTGCTCGCAGTACCAGCATCATTGC
CACCATCGGGCCAGCATCTCGCTCCGTGGAGCGCCTCAAGGAGATGATCAAGGCCGGGAT
GAACATTGCGCGACTCAACTTCTCCCACGGCTCCCACGAGTACCATGCTGAGTCCATCGC
CAACGTCCGGGAGGCGGTGGAGAGCTTTGCAGGTTCCCCACTCAGCTACCGGCCCGTGGC
CATCGCCCTGGACACCAAGGGACCGGAGATCCGCACTGGGATCCTGCAGGGGGGTCCAGA
GTCGGAAGTGGAGCTGGTGAAGGGCTCCCAGGTGCTGGTGACTGTGGACCCCGCGTTCCG
GACGCGGGGGAACGCGAACACCGTGTGGGTGGACTACCCCAATATTGTCCGGGTCGTGCC
GGTGGGGGGCCGCATCTACATTGACGACGGGCTCATCTCCCTAGTGGTCCAGAAAATCGG
CCCAGAGGGACTGGTGACCCAAGTGGAGAACGGCGGCGTCCTGGGCAGCCGGAAGGGCGT
GAACTTGCCAGGGGCCCAGGTGGACTTGCCCGGGCTGTCCGAGCAGGACGTCCGAGACCT
GCGCTTCGGGGTGGAGCATGGGGTGGACATCGTCTTTGCCTCCTTTGTGCGGAAAGCCAG
CGACGTGGCTGCCGTCAGGGCTGCTCTGGGTCCGGAAGGACACGGCATCAAGATCATCAG
CAAAATTGAGAACCACGAAGGCGTGAAGAGGTTTGATGAAATCCTGGAGGTGAGCGACGG
CATCATGGTGGCACGGGGGGACCTAGGCATCGAGATCCCAGCAGAGAAGGTTTTCCTGGC
TCAGAAGATGATGATTGGGCGCTGCAACTTGGCGGGCAAGCCTGTTGTCTGTGCCACACA
GATGCTGGAGAGCATGATTACCAAGCCCCGGCCAACGAGGGCAGAGACAAGCGATGTCGC
CAATGCTGTGCTGGATGGGGCTGACTGCATCATGCTGTCAGGGGAGACTGCCAAGGGCAA
CTTCCCTGTGGAAGCGGTGAAGATGCAGCATGCGATTGCCCGGGAGGCAGAGGCCGCAGT
GTACCACCGGCAGCTGTTTGAGGAGCTACGTCGGGCAGCGCCACTAAGCCGTGATCCCAC
TGAGGTCACCGCCATTGGTGCTGTGGAGGCTGCCTTCAAGTGCTGTGCTGCTGCCATCAT
TGTGCTGACCACAACTGGCCGCTCAGCCCAGCTTCTGTCTCGGTACCGACCTCGGGCAGC
AGTCATTGCTGTCACCCGCTCTGCCCAGGCTGCCCGCCAGGTCCACTTATGCCGAGGAGT
CTTCCCCTTGCTTTACCGTGAACCTCCAGAAGCCATCTGGGCAGATGATGTAGATCGCCG
GGTGCAATTTGGCATTGAAAGTGGAAAGCTCCGTGGCTTCCTCCGTGTTGGAGACCTGGT
GATTGTGGTGACAGGCTGGCGACCTGGCTCCGGCTACACCAACATCATGAGGGTGCTAAG
CATATCCTGAGACGCCCCTCCCCCCTCTGGCCCAGCCTACCCTTGTACCCCATCCCTTCC
TCCCCAGTCTACGTTCTCCAGCCCACACCCCTCCAAAGCCCCACCTTTAAGTCCTCTCTT
CTCTATTCCTGACCCTCCCTACCTGAGGCCTATCTGAGACTATAACTGTCATCTAGCCCC
TTCGAGGTTGCCCCTTCCCCATCTCCATTTCACACAGGTCCTGAAAGTCTGTGTCCAATT
ATGCACTGGCCACCCAACAGCACCAATTGTACATTCTCTGCATCCAATCTGCTCAGCAGG
CCCTAAGATGCCTTGAGTCTTTAATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAA

Protein sequence predicted based on nucleotide sequence

MSIQENISSLQLRSWVSKSQRDLAKSILIGAPGGPAGYLRRASVAQLTQELGTAFFQQQQ
LPAAMADTFLEHLCLLDIDSEPVAARSTSIIATIGPASRSVERLKEMIKAGMNIARLNFS
HGSHEYHAESIANVREAVESFAGSPLSYRPVAIALDTKGPEIRTGILQGGPESEVELVKG
SQVLVTVDPAFRTRGNANTVWVDYPNIVRVVPVGGRIYIDDGLISLVVQKIGPEGLVTQV
ENGGVLGSRKGVNLPGAQVDLPGLSEQDVRDLRFGVEHGVDIVFASFVRKASDVAAVRAA
LGPEGHGIKIISKIENHEGVKRFDEILEVSDGIMVARGDLGIEIPAEKVFLAQKMMIGRC
NLAGKPVVCATQMLESMITKPRPTRAETSDVANAVLDGADCIMLSGETAKGNFPVEAVKM
QHAIAREAEAAVYHRQLFEELRRAAPLSRDPTEVTAIGAVEAAFKCCAAAIIVLTTTGRS
AQLLSRYRPRAAVIAVTRSAQAARQVHLCRGVFPLLYREPPEAIWADDVDRRVQFGIESG
KLRGFLRVGDLVIVVTGWRPGSGYTNIMRVLSIS*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Host Type:  bacterial    Marker:  chloramphenicol
Bacterial Selection Condition: 100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition: Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments: Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable