DNASU Plasmid | PRKCA (H. sapiens )<br>In pLDNT7_nFLAG (Flag-tagged<br>In vitro expression vector)

Explanation of Terms

Gene:

Gene Symbol: PRKCA

Gene Name: protein kinase C, alpha

Original Clone ID:

HsCD00299378

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pLDNT7_nFLAG

Format:

CLOSED

Source:

Center for Personalized Diagnostics

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Center for Personalized Diagnostics

HsCD00617910

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 2019nts

Open reading frame: 45 to 2063

GGCCGCAGCTCCCCGGCGGAGGCAAGAGGTGGTTGGGGGGGACCATGGCTGACGTTTTCC
CGGGCAACGACTCCACGGCGTCTCAGGACGTGGCCAACCGCTTCGCCCGCAAAGGGGCGC
TGAGGCAGAAGAACGTGCACGAGGTGAAGGACCACAAATTCATCGCGCGCTTCTTCAAGC
AGCCCACCTTCTGCAGCCACTGCACCGACTTCATCTGGGGGTTTGGGAAACAAGGCTTCC
AGTGCCAAGTTTGCTGTTTTGTGGTCCACAAGAGGTGCCATGAATTTGTTACTTTTTCTT
GTCCGGGTGCGGATAAGGGACCCGACACTGATGACCCCAGGAGCAAGCACAAGTTCAAAA
TCCACACTTACGGAAGCCCCACCTTCTGCGATCACTGTGGGTCACTGCTCTATGGACTTA
TCCATCAAGGGATGAAATGTGACACCTGCGATATGAACGTTCACAAGCAATGCGTCATCA
ATGTCCCCAGCCTCTGCGGAATGGATCACACTGAGAAGAGGGGGCGGATTTACCTAAAGG
CTGAGGTTGCTGATGAAAAGCTCCATGTCACAGTACGAGATGCAAAAAATCTAATCCCTA
TGGATCCAAACGGGCTTTCAGATCCTTATGTGAAGCTGAAACTTATTCCTGATCCCAAGA
ATGAAAGCAAGCAAAAAACCAAAACCATCCGCTCCACACTAAATCCGCAGTGGAATGAGT
CCTTTACATTCAAATTGAAACCTTCAGACAAAGACCGACGACTGTCTGTAGAAATCTGGG
ACTGGGATCGAACAACAAGGAATGACTTCATGGGATCCCTTTCCTTTGGAGTTTCGGAGC
TGATGAAGATGCCGGCCAGTGGATGGTACAAGTTGCTTAACCAAGAAGAAGGTGAGTACT
ACAACGTACCCATTCCGGAAGGGGACGAGGAAGGAAACATGGAACTCAGGCAGAAATTCG
AGAAAGCCAAACTTGGCCCTGCTGGCAACAAAGTCATCAGTCCCTCTGAAGACAGGAAAC
AACCTTCCAACAACCTTGACCGAGTGAAACTCACGGACTTCAATTTCCTCATGGTGTTGG
GAAAGGGGAGTTTTGGAAAGGTGATGCTTGCCGACAGGAAGGGCACAGAAGAACTGTATG
CAATCAAAATCCTGAAGAAGGATGTGGTGATTCAGGATGATGACGTGGAGTGCACCATGG
TAGAAAAGCGAGTCTTGGCCCTGCTTGACAAACCCCCGTTCTTGACGCAGCTGCACTCCT
GCTTCCAGACAGTGGATCGGCTGTACTTCGTCATGGAATATGTCAACGGTGGGGACCTCA
TGTACCACATTCAGCAAGTAGGAAAATTTAAGGAACCACAAGCAGTATTCTATGCGGCAG
AGATTTCCATCGGATTGTTCTTTCTTCATAAAAGAGGAATCATTTATAGGGATCTGAAGT
TAGATAACGTCATGTTGGATTCAGAAGGACATATCAAAATTGCTGACTTTGGGATGTGCA
AGGAACACATGATGGATGGAGTCACGACCAGGACCTTCTGTGGGACTCCAGATTATATCG
CCCCAGAGATAATCGCTTATCAGCCGTATGGAAAATCTGTGGACTGGTGGGCCTATGGCG
TCCTGTTGTATGAAATGCTTGCCGGGCAGCCTCCATTTGATGGTGAAGATGAAGACGAGC
TATTTCAGTCTATCATGGAGCACAACGTTTCCTATCCAAAATCCTTGTCCAAGGAGGCTG
TTTCTATCTGCAAAGGACTGATGACCAAACACCCAGCCAAGCGGCTGGGCTGTGGGCCTG
AGGGGGAGAGGGACGTGAGAGAGCATGCCTTCTTCCGGAGGATCGACTGGGAAAAACTGG
AGAACAGGGAGATCCAGCCACCATTCAAGCCCAAAGTGTGTGGCAAAGGAGCAGAGAACT
TTGACAAGTTCTTCACACGAGGACAGCCCGTCTTAACACCACCTGATCAGCTGGTTATTG
CTAACATAGACCAGTCTGATTTTGAAGGGTTCTCGTATGTCAACCCCCAGTTTGTGCACC
CCATCTTACAGAGTGCAGTATGAAACTCACCAGCGAGAACAAACACCTCCCCAGCCCCCA
GCCCTCCCCGCAGTGGGAAGTGAATCCTTAACCCTAAAATTTTAAGGCCACGGCCTTGTG
TCTGATTCCATATGGAGGCCTGAAAATTGTAGGGTTATTAGTCCAAATGTGATCAACTGT
TCAGGGTCTCTCTCTTACAACCAAGAACATTATCTTAGTGGAAGATGGTACGTCATGCTC
AGTGTCCAGTTTAATTCTGTAGAAGTTACGTCTGGCTCTAGGTTAACCCTTCCTAGAAAG
CAAGCAGACTGTTGCCCCATTTTGGGTACAATTTGATATACTTTCCATACCCTCCATCTG
TGGATTTTTCAGCATTGGAATCCCCCAACCAGAGATGTTAAAGTGAGCCTGTCCCAGGAA
ACATCTCCACCCAAGACGTCTTTGGAATCCAAGAACAGGAAGCCAAGAGAGTGAGCAGGG
AGGGATTGGGGGTGGGGGAGGCCTCAAAATACCGACTGCGTCCATTCTCTGCCTCCATGG
AAACAGCCCCTAGAATCTGAAAGGCCGGGATAAACCTAATCACTGTTCCCAAACATTGAC
AAATCCTAACCCAACCATGGTCCAGCAGTTACCAGTTTAAACAAAAAAACCTCAGATGAG
TGTTGGGTGAATCTGTCATCTGGTACCCTCCTTGGTTGATAACTGTCTTGATACTTTTCA
TTCTTTGTAAGAGGCCAAATCGTCTAAGGACGTTGCTGAACAAGCGTGTGAAATCATTTC
AGATCAAGGATAAGCCAGTGTGTACATATGTTCATTTTAATCTCTGGGAGATTATTTTTC
CATCCAGGGTGCCATCAGTAATCATGCCACTACTCACCAGTGTTGTTCGCCAACACCCAC
CCCCACACACACCAACATTTTGCTGCCTACCTTGTTATCCTTCTCAAGAAGCTGAAGTGT
ACGCCCTCTCCCCTTTTGTGCTTATTTATTTAATAGGCTGCAGTGTCGCTTATGAAAGTA
CGATGTACAGTAACTTAATGGAAGTGCTGACTCTAGCATCAGCCTCTACCGATTGATTTT
CCTCCCTTCTCTAGCCCTGGATGTCCACTTAGGGATAAAAAGAATATGGTTTTGGTTCCC
ATTTCTAGTTCACGTTGAATGACAGGCCTGGAGCTGTAGAATCAGGAAACCCGGATGCCT
AACAGCTCAAAGATGTTTTGTTAATAGAAGGATTTTAATACGTTTTGCAAATGCATCATG
CAATGAATTTTGCATGTTTATAATAAACCTTAATAACAAGTGAATCTATATTATTGATAT
AATCGTATCAAGTATAAAGAGAGTATTATAATAATTTTATAAGACACAATTGTGCTCTAT
TTGTGCAGGTTCTTGTTTCTAATCCTCTTTTCTAATTAAGTTTTAGCTGAATCCCTTGCT
TCTGTGCTTTCCCTCCCTGCACATGGGCACTGTATCAGATAGATTACTTTTTAAATGTAG
ATAAAATTTCAAAAATGAATGGCTAGTTTACGTGATAGATTAGGCTCTTACTACATATGT
GTGTGTATATATATGTATTTGATTCTACCTGCAAACAAATTTTTATTGGTGAGGACTATT
TTTGAGCTGACACTCCCTCTTAGTTTCTTCATGTCACCTTTCGTCCTGGTTCCTCCGCCA
CTCTTCCTCTTGGGGACAACAGGAAGTGTCTGATTCCAGTCTGCCTAGTACGTTGGTACA
CACGTGGCATTGCCGCAGCACCTGGGCTGACCTTTGTGTGTGCGTGTGTGTGTGTTTCCT
TCTTCCCTTCAGCCTGTGACTGTTGCTGACTCCAGGGGTGGGAGGGATGGGGAGACTCCC
CTCTTGCTGTGTGTACTGGACACGCAGGAAGCATGCTGTCTTGCTGCCTCTGCAACGACC
TGTCGTTTGCTCCAGCATGCACAAACTTCGTGAGACCAACACAGCCGTGCCCTGCAGGCA
CCAGCACGTGCTTTTCAGAGGCTGCGGACTTTCTTCCAGCCATTGTGGCATTGGCCTTTC
CAGTCTTGGGAGGAGCGCGCTGCTTTGGTGAGACACCCCCATGCAAGGTCCTCAGAGTAG
CCGGGTTCTACCACAAACAGAAACAGAATGAAAGTAGCTGTCAGTCCTTGTAGAGAGCCG
CTCTGTTTCCTCCCAGAAGCATCTCCCAGCTAAGCTCGCATTATTTTTCTCCTCTGGCTG
TTTGCCTGAAGTTCACAGAACACACAACCATGAAAGGCTTTTTGAGGTGAGAGGCCCAGG
TGGTCCTGGCAACCCTGAGTAGAAGGAGAGACGGGGTAGGGAACGGGCCCGGCCAGAAAA
GAACCATTTCTTCTGCCATCTTTTATGCACCATAGACATCGAGACTCCAGGGGGTCCTGG
CTCCCCTGTCCCTGCAGCCCTGCAGGTCAGTGCATGATCTGGGTTCGTGTCCTGACCAGG
TGCTCCTCCTTTGATCCGAGGGGAAAGGGACTGGTTTATAGAAAGAGCCTAGGAGACAAA
AGGGCCAGTCCCCCTGCCCAGAATGGAGCAGCAGCAGGACAGACCCCCACGAGGCCCCCC
AGAGAGGAGGAAGATCCCACGGAGGAACACATGAGGTTAGGGACCCTTGTTCAGCACCCC
AAACAGCCTGCCTGTTTAAAGCAGGCAGCAGGCTTAGGCCTTCCCTGCAACCCCAACACC
CACAAGTTTGTTTCTCTAGGAAACACATTCACTGTCTCAGCTGGCTGTTACTCTCTCAGA
CCATATGGCAAAGTTTTCCAAGAAAATGCCCCGACAGGGGTGCCCAGCACACTGCCTGAG
GGACAACAGACATCAGAACAAACCCCCAGAGAGAAACAGTCAAAATCAGGGCCCGGTGCA
GTGTTGTCATGTGGAACCTGCTTTATCCATTGCTGAGTGTTGAATGTGGGTAATGGTTAG
GGCTTTCCAGATCTCAGCAGCCAAAGACAGTTATTGTTGGAAGACTGTCATGTAGATAAC
CATGAGCAATGGCTCGCCTCAGAATCAGTTCATAAAATTCTATGGTACTGGCCCCTTCGT
GGGTATTGTGTGAAATGAGATGGTGGCGAGGGGTGCGCTGTGGAACTGCCGCAGCCACGC
AGGAGGTCCCTGGGGGATGCTTTGGGAAGTCCTTGCCCCTGAGCACTGCCTGATTGCCAG
GGCCTGTGGAGGTCTAGGCCGCCTGGCAGAATCTAGCACCGTCCGAATCCCCGCAGGACC
CATGGAGCTATGACCACACCAGGCCATTCAAATGGCTCTGCATTATCTTCCCTTGGAAGG
TGGCCACTCCTCGGTGGCAGGGCCTTTCCCTGAGGCTGCAGGCCGTGGGCTGGCAGCCCG
TCTCTTGGCATTTCAATTGAAGGTCACCAGGTGCTGGGTTTGAAAGGAAGTCACTGGAGT
GCTGCCAGGGGCCGCCCTCCAAGGTTAATGAGAGGCCCACATCCAGGCAAGAACTAATTC
AAAAGGCAGATCAGAAACCACAGGAGTCAAAATTATTGCTCCGGCAGTGCTTCCCTTCCT
TTCATCCACTGGCCTCGTGTGGTCCATGCAGGGCCACTGTCTGCCCTTTCTGATGCCACG
TATTAGGCTTTCTTACTCAGAATTTTGATAGAAAACCATGGGGCCAAGAGCTCTGGAAGC
CTGGCCGGAAAGACCAAGGTTCATGCAGCCCAACAAATGATTGTTGAGCACCTCTCGGAG
CCAAAGTCCTTAGGCGAGTGTGGTGACTTCCTGGAAGGAGGATGCAGACTTCCAGAGAGC
CCCCCCAACGGACGTGCTGAGAAGGGAGAGGGAGGCGGGGGCTGTAGTCAGGAAGGAGCC
AGAGAAGAACAGGGTTTGGGTGCATCCAGAAATATGCCTGCAGTAGGAGGGAGAGGAAGG
GGTGCCACCGTCAACGGCTTCCCATCGGAGGTGGTTGGTGCAGATGGAAGTTTCTGTCTG
CTGGCCCTCAAGAGAGTGTTTTGCCAGGGACACAGTCTGTTCCTCCTCAGAAAACACCCC
CCAAATGCTAACAACATCCCCACCAGCTGCTAGAAGCCCCTTTCCCCTCCCCACCTTGAA
GTAGCTCATAGTTCTCTGGGCAGAGCCAGACCATCCAGTGTACCCCAGAGGCCAGTAGGT
TCCTGCCCATTTTCCTCTCTGGCTTCCTGCCAAGAATTATGGCAGCTGAGGATGAATGGA
GAAGTAAAAACAACTAACACCGCACAACTAACAACTAACACCGCAGTTCCCACCTGGGTT
CCACTTAGCAGGAGACATTTCGGAGGGTTTTTTTTGTTTTTGTTCCTGTTTTTTTTTTTT
TTGCTGGAATTTGTTTTCTCAGTACTGAAAAGAGAAAAAGTGACAATCTTGTATTTTTAA
AAGCCTCGGAAAGGTGATACCATCTGACAGTCATTTTCTCACGTTGGTCTTCTAAAGTCA
CCTATTTCTTGTGTGTGCACATCACACCATTTCCTGTTTCTTTATAACCCGACAAGGGTA
GGAGTGCCTGTTTCCCCTGCTGGGCACACCAGACAATCGTAATCACAAAACAGACACTGA
GCCAGGGGCCCAAAGGGTGTGATCATGAGAGTTACCGGGACAGCAGTAGGCATGACAGTC
ACCAGGAAGGACAAGGGTGCTCTGTTGTTAGTGGCCACACACCAATTTGACAAGGAGTGT
TGCGAAATTTTTATTTATTTATTTATTTATTTTGAGATGGAGTTTCACTCTTGTTGCCCA
GGCTGGAGTGCGGTGGTACAATCTCGGCTCACTGCAACCTCCACCTCCCAGGTTCAAGCG
ATTCTCCTGCCTCAGCCTCCCAAGTACCTGGGACTACAGGTGCGTGCCACCACACCCAGC
TAAATTTTGTGTTTTTAGTAGAGATGGGGTTTCACCATGTTGGCCAGGATGGTCTTGAAC
CCCTGACCTCATGATCTGCCTGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGC
CACCACGCCCAGCCAAAATATTTTTTTAAAGTCATTTTCCTTAAGCTGCTTGGGCTACAT
GTGAAATACACTGGACGGTCAACATTCCTGTCTCCTCCCATTTGGGCTGATGCAGCAGAT
CCAGGGAATGTTACCTGTTTCTGCTGCTAGAAGATCCAGGAAATTGGGAAGGTTACCTGA
CGCACACATGGATGAAGGCCATCATCTAGAAATGGGGTCAACCACAATTGTGTTAATTCC
GTAGTGTCAGGGATTCTTCGGGAAGGTCAACAGTATGAAGGATTCTGACCCCTGTGCCTC
CCATTTATGTGATCAGGTGACAGTTAATAACCGTGGAGGTCACACTCAGCCATCCAACAG
CCTTACAGTGACCCTACACAAAAGCCCCCAAATTCCAAAGACTTTTTCTTAACCTAAAGG
AAGAAATTATTTGTTAATTCCAGTAGAGCAACTGAATATACTGGGCTATTTGTACTTTTT
TATAGAGAACTTTAATAATAATTCTTTAAAAATGAGTTTTTAGAACAAAGCAACTGACGA
TTTCCTAAGATTCCAATGCCCTGGAGCTTGTAGGAGGACTTAGCCTGGGTCAGCTGGAGC
ACCCCCGACCTGATCTCCCACTGCCAGATTTTCCCATGCTCCTAGGGTATGGAGTCCACG
TGGGAATGACTGCAAGTTCAGGTGGAACTTGGCCGACTGATGCTCTGCGAGTTTTTAATA
GACACTGGGGACAACTGCTTAAGGTTTAGAAACTTCCAAACCACAGGAAAGACATTTTTA
GTGTCCCCCATCCAGAGGCAGCCCTGGAATAGGATTCCCAGGGGTTTCTGGGACCCCTTT
CCTTGCTCCGTGAGGCTCTGTGGCCATCTTTTGGCAGGAGGAGGATGCTTCCTTGGCTCT
GTGCCCAGACCCGCCTGGTCCCCAGGTCTCTCACCTTGGGTGAAGATTCAGAGATGCCCT
GTAAGGATTTTGCCCACTGGGCAACTCAGAAATACTTCGATCTCCCAAGATATAAGAGGC
AGCAGCAAACGTGCCTATTGACGTCTGTTTCATAGTTACCACTTACGCGAGTAGACAGAA
CTCGGCTTTTCAGAAAATAGGTGTCAAGTCCACTTTATAAGAACCTTTTTTTCTAAAATA
AGATAAAAGGTGGCTTTGCATTTTCTGATTAAACGACTGTGTCTTTGTCACCTCTGCTTA
ACTTTAGGAGTATCCATTCCTGTGATTGTAGACTTTTGTTGATATTCTTCCTGGAAGAAT
ATCATTCTTTTCTTGAAGGGTTGGTTTACTAGAATATTCAAAATCAATCATGAAGGCAGT
TACTATTTTGAGTCTAAAGGTTTTCTAAAAATTAACCTCACATCCCTTCTGTTAGGGTCT
TTCAGAATATCTTTTATAAACAGAAGCATTTGAAGTCATTGCTTTTGCTACATGATTTGT
GTGTGTGAAGGACATACCACGTTTAAATCATTAATTGAAAAACATCATATAAGCCCCAAC
TTTGTTTGGAGGAAGAGACGGAGGTTGAGGTTTTTCCTTCTGTATAAGCACCTACTGACA
AAATGTAGAGGCCATTCAACCGTCAAACACCATTTGGTTATATCGCAGAGGAGACGGATG
TGTAAATTACTGCATTGCTTTTTTTTTCAGTTTGTATAACCTCTAATCTCCGTTTGCATG
ATACGCTTTGTTAGAAACATTAATTGTAGTTTGGAAGCAAGTGTGTATGAATAAAGATAA
TGATCATTCCAAAAAAAAAAAAAAAAA

Protein sequence predicted based on nucleotide sequence

MADVFPGNDSTASQDVANRFARKGALRQKNVHEVKDHKFIARFFKQPTFCSHCTDFIWGF
GKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPDTDDPRSKHKFKIHTYGSPTFCDHCGS
LLYGLIHQGMKCDTCDMNVHKQCVINVPSLCGMDHTEKRGRIYLKAEVADEKLHVTVRDA
KNLIPMDPNGLSDPYVKLKLIPDPKNESKQKTKTIRSTLNPQWNESFTFKLKPSDKDRRL
SVEIWDWDRTTRNDFMGSLSFGVSELMKMPASGWYKLLNQEEGEYYNVPIPEGDEEGNME
LRQKFEKAKLGPAGNKVISPSEDRKQPSNNLDRVKLTDFNFLMVLGKGSFGKVMLADRKG
TEELYAIKILKKDVVIQDDDVECTMVEKRVLALLDKPPFLTQLHSCFQTVDRLYFVMEYV
NGGDLMYHIQQVGKFKEPQAVFYAAEISIGLFFLHKRGIIYRDLKLDNVMLDSEGHIKIA
DFGMCKEHMMDGVTTRTFCGTPDYIAPEIIAYQPYGKSVDWWAYGVLLYEMLAGQPPFDG
EDEDELFQSIMEHNVSYPKSLSKEAVSICKGLMTKHPAKRLGCGPEGERDVREHAFFRRI
DWEKLENREIQPPFKPKVCGKGAENFDKFFTRGQPVLTPPDQLVIANIDQSDFEGFSYVN
PQFVHPILQSAV*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin
Host Type: bacterial Marker: chloramphenicol

Bacterial Selection Condition: 100 ug/mL ampicillin, 34 ug/mL chloramphenicol

Growth Condition: Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.

Comments: Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pLDNT7_nFLAG

Synonyms:

pLDNT7-Flag

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning

Comments:

None

Size (bp):

4557

Parent Vector:

None

Properties:

Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker

Author Name:

Daniel Schiwek

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

GGGCGAATTAATTCCGGTTATTTTCCACCATATTGCCGTCTTTTGGCAATGTGAGGGCCCGGAAACCTGGCCCTGTCTTCTTGACGAGCATTCCTAGGGGTCTTTCCCCTCTCGCCAAAGGAATGCAAGGTCTGTTGAATGTCGTGAAGGAAGCAGTTCCTCTGGAAGCTTCTTGAAGACAAACAACGTCTGTAGCGACCCTTTGCAGGCAGCGGAACCCCCCACCTGGCGACAGGTGCCTCTGCGGCCAAAAGCCACGTGTATAAGATACACCTGCAAAGGCGGCACAACCCCAGTGCCACGTTGTGAGTTGGATAGTTGTGGAAAGAGTCAAATGGCTCACCTCAAGCGTATTCAACAAGGGGCTGAAGGATGCCCAGAAGGTACCCCATTGTATGGGATCTGATCTGGGGCCTCGGTGCACATGCTTTACATGTGTTTAGTCGAGGTTAAAAAACGTCTAGGCCCCCCGAACCACGGGGACGTGGTTTTCCTTTGAAAAACACGATGATAATATGGATCGGATCCGAATTCGAGCTCCGTCATCAACAAGTTTGTACAAAAAAGCAGGCTCCACCATGGCCGACTACAAGGACCACGACGGCGACTACAAGGACCACGACATCGACTACAAGGACGACGACGACAAGAGATCTATAACTTCGTATAGCATACATTATACGAAGTTATTGATCCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTTTGGACCCAGCTTTCTTGTACAAAGTGGTTGATGACAAGCTTGCGGCCGCACTCGAGCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCACTCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATGAGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTGGAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACAGTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTGGTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTGGATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAACTCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCGAAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCATCCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCCAATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTGAAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCATGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCCAAAATCGGATCTGGTTCCGCGTTGAAGATCTGACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTTTAAACACTAGTCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGAAATTGTAAACGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATTGTAATACGACTCACTATA

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ColE1 ori

ColE1 origin of replication

1842

2524

promoter

T7 promoter

4462

4479

recombination site

LoxP

LoxP site for recombinational cloning

657

690

selectable marker

AmpR

ampicillin resistance gene

2622

3281

ssDNA origin

f1 ori

f1 origin for ssDNA production

3928

4368

tag

Flag

3xFlag epitope tag ORF and LoxP region

585

650

trxn termination sequence

T7 term

T7 terminator sequence

1614

1660

Gene

Gene Symbol :

PRKCA

Symbol Nomenclature :

PRKCA

SYNONYM :

AAG6; PKC-alpha; PKCA; PRKACA

Designation :

PKC-A|aging-associated gene 6|protein kinase C alpha type

Full Nomenclature :

protein kinase C, alpha

GENEID :

5578

HGNC :

9393

MIM :

176960

Vega :

OTTHUMG00000179533

Target GenBank:

NM_002737

Pathways

NCI :

a6b1 and a6b4 Integrin signaling

NCI :

ATF-2 transcription factor network

NCI :

Canonical NF-kappaB pathway

NCI :

Downstream signaling in naïve CD8+ T cells

NCI :

Endothelins

NCI :

ErbB1 downstream signaling

NCI :

IL8- and CXCR1-mediated signaling events

NCI :

IL8- and CXCR2-mediated signaling events

NCI :

mTOR signaling pathway

NCI :

PAR1-mediated thrombin signaling events

NCI :

PDGFR-beta signaling pathway

NCI :

Ras signaling in the CD4+ TCR pathway

NCI :

Regulation of Ras family activation

NCI :

Retinoic acid receptors-mediated signaling

NCI :

Role of Calcineurin-dependent NFAT signaling in lymphocytes

NCI :

Signaling events mediated by VEGFR1 and VEGFR2

NCI :

Signaling events regulated by Ret tyrosine kinase

NCI :

Syndecan-4-mediated signaling events

NCI :

TCR signaling in naïve CD4+ T cells

NCI :

TCR signaling in naïve CD8+ T cells

NCI :

Thromboxane A2 receptor signaling

NCI :

VEGFR1 specific signals

Panther :

5HT2 type receptor mediated signaling pathway

Panther :

Alpha adrenergic receptor signaling pathway

Panther :

Alzheimer disease-amyloid secretase pathway

Panther :

Angiogenesis

Panther :

Angiotensin II-stimulated signaling through G proteins and beta-arrestin

Panther :

Apoptosis signaling pathway

Panther :

EGF receptor signaling pathway

Panther :

Endothelin signaling pathway

Panther :

FGF signaling pathway

Panther :

Heterotrimeric G-protein signaling pathway-Gq alpha and Go alpha mediated pathway

Panther :

Histamine H1 receptor mediated signaling pathway

Panther :

Muscarinic acetylcholine receptor 1 and 3 signaling pathway

Panther :

Oxytocin receptor mediated signaling pathway

Panther :

PDGF signaling pathway

Panther :

Thyrotropin-releasing hormone receptor signaling pathway

Panther :

VEGF signaling pathway

Panther :

Wnt signaling pathway

Reactome :

Acetylcholine regulates insulin secretion

Reactome :

beta-catenin independent WNT signaling

Reactome :

Ca-dependent events

Reactome :

Ca2+ pathway

Reactome :

Calmodulin induced events

Reactome :

CaM pathway

Reactome :

Cell Cycle

Reactome :

Cell Cycle, Mitotic

Reactome :

DAG and IP3 signaling

Reactome :

DAP12 interactions

Reactome :

DAP12 signaling

Reactome :

Depolymerisation of the Nuclear Lamina

Reactome :

Disease

Reactome :

Diseases associated with visual transduction

Reactome :

Disinhibition of SNARE formation

Reactome :

Downstream signal transduction

Reactome :

Downstream signaling of activated FGFR

Reactome :

EGFR interacts with phospholipase C-gamma

Reactome :

EGFR Transactivation by Gastrin

Reactome :

Extracellular matrix organization

Reactome :

G alpha (z) signalling events

Reactome :

G-protein mediated events

Reactome :

Gastrin-CREB signalling pathway via PKC and MAPK

Reactome :

Gene Expression

Reactome :

Glutamate Binding, Activation of AMPA Receptors and Synaptic Plasticity

Reactome :

GPCR downstream signaling

Reactome :

Hemostasis

Reactome :

HuR stabilizes mRNA

Reactome :

Immune System

Reactome :

Inactivation, recovery and regulation of the phototransduction cascade

Reactome :

Innate Immune System

Reactome :

Integration of energy metabolism

Reactome :

M Phase

Reactome :

Metabolism

Reactome :

Metabolism of mRNA

Reactome :

Metabolism of RNA

Reactome :

Mitotic M-M/G1 phases

Reactome :

Mitotic Prophase

Reactome :

Neuronal System

Reactome :

Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell

Reactome :

NGF signalling via TRKA from the plasma membrane

Reactome :

Non-integrin membrane-ECM interactions

Reactome :

Nuclear Envelope Breakdown

Reactome :

Opioid Signalling

Reactome :

PCP/CE pathway

Reactome :

Phospholipase C-mediated cascade

Reactome :

Platelet activation, signaling and aggregation

Reactome :

PLC beta mediated events

Reactome :

PLC-gamma1 signalling

Reactome :

PLCG1 events in ERBB2 signaling

Reactome :

Regulation of insulin secretion

Reactome :

Regulation of KIT signaling

Reactome :

Regulation of mRNA stability by proteins that bind AU-rich elements

Reactome :

Response to elevated platelet cytosolic Ca2+

Reactome :

Signal Transduction

Reactome :

Signaling by EGFR

Reactome :

Signaling by EGFR in Cancer

Reactome :

Signaling by ERBB2

Reactome :

Signaling by FGFR

Reactome :

Signaling by FGFR in disease

Reactome :

Signaling by GPCR

Reactome :

Signaling by PDGF

Reactome :

Signaling by SCF-KIT

Reactome :

Signaling by Wnt

Reactome :

Signalling by NGF

Reactome :

Syndecan interactions

Reactome :

The phototransduction cascade

Reactome :

Trafficking of AMPA receptors

Reactome :

Trafficking of GluR2-containing AMPA receptors

Reactome :

Transmission across Chemical Synapses

Reactome :

Visual phototransduction

Reactome :

WNT5A-dependent internalization of FZD4

WikiPathway :

Alpha 6 Beta 4 signaling pathway

WikiPathway :

angiogenesis overview

WikiPathway :

Calcium Regulation in the Cardiac Cell

WikiPathway :

EGF/EGFR Signaling Pathway

WikiPathway :

ErbB signaling pathway

WikiPathway :

FSH signaling pathway

WikiPathway :

G Protein Signaling Pathways

WikiPathway :

Insulin Signaling

WikiPathway :

Keap1-Nrf2 Pathway

WikiPathway :

Kit receptor signaling pathway

WikiPathway :

miRs in Muscle Cell Differentiation

WikiPathway :

Myometrial Relaxation and Contraction Pathways

WikiPathway :

TOR signaling

WikiPathway :

Wnt Signaling Pathway

WikiPathway :

Wnt Signaling Pathway and Pluripotency

Protein

SMART domain :

C2 : Protein kinase C conserved region 2 (CalB)

SMART domain :

C1 : Protein kinase C conserved region 1 (C1) domains (Cysteine-rich domains)

SMART domain :

TyrKc : Tyrosine kinase, catalytic domain

SMART domain :

S_TK_X : Extension to Ser/Thr-type protein kinases

SMART domain :

S_TKc : Serine/Threonine protein kinases, catalytic domain

UniProt :

P17252

UniProt :

Q7Z727

HPRD :

01498

Clone

Cloning Information :

654638

HIP Master Clone ID :

136423

Original Clone ID :

FLH231619.01L

Original Clone ID :

HsCD00299378

Organism

Species Specific ID:

5578

Genome

Chromosome :

Map Location :

17q22-q23.2

Ensembl :

ENSG00000154229

Reagents

Labome :

PKC-alpha-antibody

PRKCA (H. sapiens )In pLDNT7_nFLAG (Flag-taggedIn vitro expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Pathways

Protein

Clone

Organism

Genome

Reagents

PRKCA (H. sapiens )
In pLDNT7_nFLAG (Flag-tagged
In vitro expression vector)