Gene:
Gene Symbol:
FLT1
Gene Name:
fms-related tyrosine kinase 1
Original Clone ID:
FLH230198.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00617917
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
GCGGACACTCCTCTCGGCTCCTCCCCGGCAGCGGCGGCGGCTCGGAGCGGGCTCCGGGGC
TCGGGTGCAGCGGCCAGCGGGCCTGGCGGCGAGGATTACCCGGGGAAGTGGTTGTCTCCT
GGCTGGAGCCGCGAGACGGGCGCTCAGGGCGCGGGGCCGGCGGCGGCGAACGAGAGGACG
GACTCTGGCGGCCGGGTCGTTGGCCGGGGGAGCGCGGGCACCGGGCGAGCAGGCCGCGTC
GCGCTCACCATGGTCAGCTACTGGGACACCGGGGTCCTGCTGTGCGCGCTGCTCAGCTGT
CTGCTTCTCACAGGATCTAGTTCAGGTTCAAAATTAAAAGATCCTGAACTGAGTTTAAAA
GGCACCCAGCACATCATGCAAGCAGGCCAGACACTGCATCTCCAATGCAGGGGGGAAGCA
GCCCATAAATGGTCTTTGCCTGAAATGGTGAGTAAGGAAAGCGAAAGGCTGAGCATAACT
AAATCTGCCTGTGGAAGAAATGGCAAACAATTCTGCAGTACTTTAACCTTGAACACAGCT
CAAGCAAACCACACTGGCTTCTACAGCTGCAAATATCTAGCTGTACCTACTTCAAAGAAG
AAGGAAACAGAATCTGCAATCTATATATTTATTAGTGATACAGGTAGACCTTTCGTAGAG
ATGTACAGTGAAATCCCCGAAATTATACACATGACTGAAGGAAGGGAGCTCGTCATTCCC
TGCCGGGTTACGTCACCTAACATCACTGTTACTTTAAAAAAGTTTCCACTTGACACTTTG
ATCCCTGATGGAAAACGCATAATCTGGGACAGTAGAAAGGGCTTCATCATATCAAATGCA
ACGTACAAAGAAATAGGGCTTCTGACCTGTGAAGCAACAGTCAATGGGCATTTGTATAAG
ACAAACTATCTCACACATCGACAAACCAATACAATCATAGATGTCCAAATAAGCACACCA
CGCCCAGTCAAATTACTTAGAGGCCATACTCTTGTCCTCAATTGTACTGCTACCACTCCC
TTGAACACGAGAGTTCAAATGACCTGGAGTTACCCTGATGAAAAAAATAAGAGAGCTTCC
GTAAGGCGACGAATTGACCAAAGCAATTCCCATGCCAACATATTCTACAGTGTTCTTACT
ATTGACAAAATGCAGAACAAAGACAAAGGACTTTATACTTGTCGTGTAAGGAGTGGACCA
TCATTCAAATCTGTTAACACCTCAGTGCATATATATGATAAAGCATTCATCACTGTGAAA
CATCGAAAACAGCAGGTGCTTGAAACCGTAGCTGGCAAGCGGTCTTACCGGCTCTCTATG
AAAGTGAAGGCATTTCCCTCGCCGGAAGTTGTATGGTTAAAAGATGGGTTACCTGCGACT
GAGAAATCTGCTCGCTATTTGACTCGTGGCTACTCGTTAATTATCAAGGACGTAACTGAA
GAGGATGCAGGGAATTATACAATCTTGCTGAGCATAAAACAGTCAAATGTGTTTAAAAAC
CTCACTGCCACTCTAATTGTCAATGTGAAACCCCAGATTTACGAAAAGGCCGTGTCATCG
TTTCCAGACCCGGCTCTCTACCCACTGGGCAGCAGACAAATCCTGACTTGTACCGCATAT
GGTATCCCTCAACCTACAATCAAGTGGTTCTGGCACCCCTGTAACCATAATCATTCCGAA
GCAAGGTGTGACTTTTGTTCCAATAATGAAGAGTCCTTTATCCTGGATGCTGACAGCAAC
ATGGGAAACAGAATTGAGAGCATCACTCAGCGCATGGCAATAATAGAAGGAAAGAATAAG
ATGGCTAGCACCTTGGTTGTGGCTGACTCTAGAATTTCTGGAATCTACATTTGCATAGCT
TCCAATAAAGTTGGGACTGTGGGAAGAAACATAAGCTTTTATATCACAGATGTGCCAAAT
GGGTTTCATGTTAACTTGGAAAAAATGCCGACGGAAGGAGAGGACCTGAAACTGTCTTGC
ACAGTTAACAAGTTCTTATACAGAGACGTTACTTGGATTTTACTGCGGACAGTTAATAAC
AGAACAATGCACTACAGTATTAGCAAGCAAAAAATGGCCATCACTAAGGAGCACTCCATC
ACTCTTAATCTTACCATCATGAATGTTTCCCTGCAAGATTCAGGCACCTATGCCTGCAGA
GCCAGGAATGTATACACAGGGGAAGAAATCCTCCAGAAGAAAGAAATTACAATCAGAGAT
CAGGAAGCACCATACCTCCTGCGAAACCTCAGTGATCACACAGTGGCCATCAGCAGTTCC
ACCACTTTAGACTGTCATGCTAATGGTGTCCCCGAGCCTCAGATCACTTGGTTTAAAAAC
AACCACAAAATACAACAAGAGCCTGGAATTATTTTAGGACCAGGAAGCAGCACGCTGTTT
ATTGAAAGAGTCACAGAAGAGGATGAAGGTGTCTATCACTGCAAAGCCACCAACCAGAAG
GGCTCTGTGGAAAGTTCAGCATACCTCACTGTTCAAGGAACCTCGGACAAGTCTAATCTG
GAGCTGATCACTCTAACATGCACCTGTGTGGCTGCGACTCTCTTCTGGCTCCTATTAACC
CTCCTTATCCGAAAAATGAAAAGGTCTTCTTCTGAAATAAAGACTGACTACCTATCAATT
ATAATGGACCCAGATGAAGTTCCTTTGGATGAGCAGTGTGAGCGGCTCCCTTATGATGCC
AGCAAGTGGGAGTTTGCCCGGGAGAGACTTAAACTGGGCAAATCACTTGGAAGAGGGGCT
TTTGGAAAAGTGGTTCAAGCATCAGCATTTGGCATTAAGAAATCACCTACGTGCCGGACT
GTGGCTGTGAAAATGCTGAAAGAGGGGGCCACGGCCAGCGAGTACAAAGCTCTGATGACT
GAGCTAAAAATCTTGACCCACATTGGCCACCATCTGAACGTGGTTAACCTGCTGGGAGCC
TGCACCAAGCAAGGAGGGCCTCTGATGGTGATTGTTGAATACTGCAAATATGGAAATCTC
TCCAACTACCTCAAGAGCAAACGTGACTTATTTTTTCTCAACAAGGATGCAGCACTACAC
ATGGAGCCTAAGAAAGAAAAAATGGAGCCAGGCCTGGAACAAGGCAAGAAACCAAGACTA
GATAGCGTCACCAGCAGCGAAAGCTTTGCGAGCTCCGGCTTTCAGGAAGATAAAAGTCTG
AGTGATGTTGAGGAAGAGGAGGATTCTGACGGTTTCTACAAGGAGCCCATCACTATGGAA
GATCTGATTTCTTACAGTTTTCAAGTGGCCAGAGGCATGGAGTTCCTGTCTTCCAGAAAG
TGCATTCATCGGGACCTGGCAGCGAGAAACATTCTTTTATCTGAGAACAACGTGGTGAAG
ATTTGTGATTTTGGCCTTGCCCGGGATATTTATAAGAACCCCGATTATGTGAGAAAAGGA
GATACTCGACTTCCTCTGAAATGGATGGCTCCCGAATCTATCTTTGACAAAATCTACAGC
ACCAAGAGCGACGTGTGGTCTTACGGAGTATTGCTGTGGGAAATCTTCTCCTTAGGTGGG
TCTCCATACCCAGGAGTACAAATGGATGAGGACTTTTGCAGTCGCCTGAGGGAAGGCATG
AGGATGAGAGCTCCTGAGTACTCTACTCCTGAAATCTATCAGATCATGCTGGACTGCTGG
CACAGAGACCCAAAAGAAAGGCCAAGATTTGCAGAACTTGTGGAAAAACTAGGTGATTTG
CTTCAAGCAAATGTACAACAGGATGGTAAAGACTACATCCCAATCAATGCCATACTGACA
GGAAATAGTGGGTTTACATACTCAACTCCTGCCTTCTCTGAGGACTTCTTCAAGGAAAGT
ATTTCAGCTCCGAAGTTTAATTCAGGAAGCTCTGATGATGTCAGATATGTAAATGCTTTC
AAGTTCATGAGCCTGGAAAGAATCAAAACCTTTGAAGAACTTTTACCGAATGCCACCTCC
ATGTTTGATGACTACCAGGGCGACAGCAGCACTCTGTTGGCCTCTCCCATGCTGAAGCGC
TTCACCTGGACTGACAGCAAACCCAAGGCCTCGCTCAAGATTGACTTGAGAGTAACCAGT
AAAAGTAAGGAGTCGGGGCTGTCTGATGTCAGCAGGCCCAGTTTCTGCCATTCCAGCTGT
GGGCACGTCAGCGAAGGCAAGCGCAGGTTCACCTACGACCACGCTGAGCTGGAAAGGAAA
ATCGCGTGCTGCTCCCCGCCCCCAGACTACAACTCGGTGGTCCTGTACTCCACCCCACCC
ATCTAGAGTTTGACACGAAGCCTTATTTCTAGAAGCACATGTGTATTTATACCCCCAGGA
AACTAGCTTTTGCCAGTATTATGCATATATAAGTTTACACCTTTATCTTTCCATGGGAGC
CAGCTGCTTTTTGTGATTTTTTTAATAGTGCTTTTTTTTTTTGACTAACAAGAATGTAAC
TCCAGATAGAGAAATAGTGACAAGTGAAGAACACTACTGCTAAATCCTCATGTTACTCAG
TGTTAGAGAAATCCTTCCTAAACCCAATGACTTCCCTGCTCCAACCCCCGCCACCTCAGG
GCACGCAGGACCAGTTTGATTGAGGAGCTGCACTGATCACCCAATGCATCACGTACCCCA
CTGGGCCAGCCCTGCAGCCCAAAACCCAGGGCAACAAGCCCGTTAGCCCCAGGGGATCAC
TGGCTGGCCTGAGCAACATCTCGGGAGTCCTCTAGCAGGCCTAAGACATGTGAGGAGGAA
AAGGAAAAAAAGCAAAAAGCAAGGGAGAAAAGAGAAACCGGGAGAAGGCATGAGAAAGAA
TTTGAGACGCACCATGTGGGCACGGAGGGGGACGGGGCTCAGCAATGCCATTTCAGTGGC
TTCCCAGCTCTGACCCTTCTACATTTGAGGGCCCAGCCAGGAGCAGATGGACAGCGATGA
GGGGACATTTTCTGGATTCTGGGAGGCAAGAAAAGGACAAATATCTTTTTTGGAACTAAA
GCAAATTTTAGACCTTTACCTATGGAAGTGGTTCTATGTCCATTCTCATTCGTGGCATGT
TTTGATTTGTAGCACTGAGGGTGGCACTCAACTCTGAGCCCATACTTTTGGCTCCTCTAG
TAAGATGCACTGAAAACTTAGCCAGAGTTAGGTTGTCTCCAGGCCATGATGGCCTTACAC
TGAAAATGTCACATTCTATTTTGGGTATTAATATATAGTCCAGACACTTAACTCAATTTC
TTGGTATTATTCTGTTTTGCACAGTTAGTTGTGAAAGAAAGCTGAGAAGAATGAAAATGC
AGTCCTGAGGAGAGTTTTCTCCATATCAAAACGAGGGCTGATGGAGGAAAAAGGTCAATA
AGGTCAAGGGAAGACCCCGTCTCTATACCAACCAAACCAATTCACCAACACAGTTGGGAC
CCAAAACACAGGAAGTCAGTCACGTTTCCTTTTCATTTAATGGGGATTCCACTATCTCAC
ACTAATCTGAAAGGATGTGGAAGAGCATTAGCTGGCGCATATTAAGCACTTTAAGCTCCT
TGAGTAAAAAGGTGGTATGTAATTTATGCAAGGTATTTCTCCAGTTGGGACTCAGGATAT
TAGTTAATGAGCCATCACTAGAAGAAAAGCCCATTTTCAACTGCTTTGAAACTTGCCTGG
GGTCTGAGCATGATGGGAATAGGGAGACAGGGTAGGAAAGGGCGCCTACTCTTCAGGGTC
TAAAGATCAAGTGGGCCTTGGATCGCTAAGCTGGCTCTGTTTGATGCTATTTATGCAAGT
TAGGGTCTATGTATTTA
Protein sequence predicted based on nucleotide sequence
MVSYWDTGVLLCALLSCLLLTGSSSGSKLKDPELSLKGTQHIMQAGQTLHLQCRGEAAHK WSLPEMVSKESERLSITKSACGRNGKQFCSTLTLNTAQANHTGFYSCKYLAVPTSKKKET ESAIYIFISDTGRPFVEMYSEIPEIIHMTEGRELVIPCRVTSPNITVTLKKFPLDTLIPD GKRIIWDSRKGFIISNATYKEIGLLTCEATVNGHLYKTNYLTHRQTNTIIDVQISTPRPV KLLRGHTLVLNCTATTPLNTRVQMTWSYPDEKNKRASVRRRIDQSNSHANIFYSVLTIDK MQNKDKGLYTCRVRSGPSFKSVNTSVHIYDKAFITVKHRKQQVLETVAGKRSYRLSMKVK AFPSPEVVWLKDGLPATEKSARYLTRGYSLIIKDVTEEDAGNYTILLSIKQSNVFKNLTA TLIVNVKPQIYEKAVSSFPDPALYPLGSRQILTCTAYGIPQPTIKWFWHPCNHNHSEARC DFCSNNEESFILDADSNMGNRIESITQRMAIIEGKNKMASTLVVADSRISGIYICIASNK VGTVGRNISFYITDVPNGFHVNLEKMPTEGEDLKLSCTVNKFLYRDVTWILLRTVNNRTM HYSISKQKMAITKEHSITLNLTIMNVSLQDSGTYACRARNVYTGEEILQKKEITIRDQEA PYLLRNLSDHTVAISSSTTLDCHANGVPEPQITWFKNNHKIQQEPGIILGPGSSTLFIER VTEEDEGVYHCKATNQKGSVESSAYLTVQGTSDKSNLELITLTCTCVAATLFWLLLTLLI RKMKRSSSEIKTDYLSIIMDPDEVPLDEQCERLPYDASKWEFARERLKLGKSLGRGAFGK VVQASAFGIKKSPTCRTVAVKMLKEGATASEYKALMTELKILTHIGHHLNVVNLLGACTK QGGPLMVIVEYCKYGNLSNYLKSKRDLFFLNKDAALHMEPKKEKMEPGLEQGKKPRLDSV TSSESFASSGFQEDKSLSDVEEEEDSDGFYKEPITMEDLISYSFQVARGMEFLSSRKCIH RDLAARNILLSENNVVKICDFGLARDIYKNPDYVRKGDTRLPLKWMAPESIFDKIYSTKS DVWSYGVLLWEIFSLGGSPYPGVQMDEDFCSRLREGMRMRAPEYSTPEIYQIMLDCWHRD PKERPRFAELVEKLGDLLQANVQQDGKDYIPINAILTGNSGFTYSTPAFSEDFFKESISA PKFNSGSSDDVRYVNAFKFMSLERIKTFEELLPNATSMFDDYQGDSSTLLASPMLKRFTW TDSKPKASLKIDLRVTSKSKESGLSDVSRPSFCHSSCGHVSEGKRRFTYDHAELERKIAC CSPPPDYNSVVLYSTPPI*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: chloramphenicol
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
