Gene:
Gene Symbol:
PTK2
Gene Name:
protein tyrosine kinase 2
Original Clone ID:
HsCD00299398
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00617920
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3021nts
Open reading frame: 32 to 3052
ATATGACAGATACCTAGCATCTAGCAAAATAATGGCAGCTGCTTACCTTGACCCCAACTT
GAATCACACACCAAATTCGAGTACTAAGACTCACCTGGGTACTGGTATGGAACGTTCTCC
TGGTGCAATGGAGCGAGTATTAAAGGTCTTTCATTATTTTGAAAGCAATAGTGAGCCAAC
CACCTGGGCCAGTATTATCAGGCATGGAGATGCTACTGATGTCAGGGGCATCATTCAGAA
GATAGTGGACAGTCACAAAGTAAAGCATGTGGCCTGCTATGGATTCCGCCTCAGTCACCT
GCGGTCAGAGGAGGTTCACTGGCTTCACGTGGATATGGGCGTCTCCAGTGTGAGGGAGAA
GTATGAGCTTGCTCACCCACCAGAGGAGTGGAAATATGAATTGAGAATTCGTTATTTGCC
AAAAGGATTTCTAAACCAGTTTACTGAAGATAAGCCAACTTTGAATTTCTTCTATCAACA
GGTGAAGAGCGATTATATGTTAGAGATAGCTGATCAAGTGGACCAGGAAATTGCTTTGAA
GTTGGGTTGTCTAGAAATACGGCGATCATACTGGGAGATGCGGGGCAATGCACTAGAAAA
GAAGTCTAACTATGAAGTATTAGAAAAAGATGTTGGTTTAAAGCGATTTTTTCCTAAGAG
TTTACTGGATTCTGTCAAGGCCAAAACACTAAGAAAACTGATCCAACAAACATTTAGACA
ATTTGCCAACCTTAATAGAGAAGAAAGTATTCTGAAATTCTTTGAGATCCTGTCTCCAGT
CTACAGATTTGATAAGGAATGCTTCAAGTGTGCTCTTGGTTCAAGCTGGATTATTTCAGT
GGAACTGGCAATCGGCCCAGAAGAAGGAATCAGTTACCTAACGGACAAGGGCTGCAATCC
CACACATCTTGCTGACTTCACTCAAGTGCAAACCATTCAGTATTCAAACAGTGAAGACAA
GGACAGAAAAGGAATGCTACAACTAAAAATAGCAGGTGCACCCGAGCCTCTGACAGTGAC
GGCACCATCCCTAACCATTGCGGAGAATATGGCTGACCTAATAGATGGGTACTGCCGGCT
GGTGAATGGAACCTCGCAGTCATTTATCATCAGACCTCAGAAAGAAGGTGAACGGGCTTT
GCCATCAATACCAAAGTTGGCCAACAGCGAAAAGCAAGGCATGCGGACACACGCCGTCTC
TGTGTCAGAAACAGATGATTATGCTGAGATTATAGATGAAGAAGATACTTACACCATGCC
CTCAACCAGGGATTATGAGATTCAAAGAGAAAGAATAGAACTTGGACGATGTATTGGAGA
AGGCCAATTTGGAGATGTACATCAAGGCATTTATATGAGTCCAGAGAATCCAGCTTTGGC
GGTTGCAATTAAAACATGTAAAAACTGTACTTCGGACAGCGTGAGAGAGAAATTTCTTCA
AGAAGCCTTAACAATGCGTCAGTTTGACCATCCTCATATTGTGAAGCTGATTGGAGTCAT
CACAGAGAATCCTGTCTGGATAATCATGGAGCTGTGCACACTTGGAGAGCTGAGGTCATT
TTTGCAAGTAAGGAAATACAGTTTGGATCTAGCATCTTTGATCCTGTATGCCTATCAGCT
TAGTACAGCTCTTGCATATCTAGAGAGCAAAAGATTTGTACACAGGGACATTGCTGCTCG
GAATGTTCTGGTGTCCTCAAATGATTGTGTAAAATTAGGAGACTTTGGATTATCCCGATA
TATGGAAGATAGTACTTACTACAAAGCTTCCAAAGGAAAATTGCCTATTAAATGGATGGC
TCCAGAGTCAATCAATTTTCGACGTTTTACCTCAGCTAGTGACGTATGGATGTTTGGTGT
GTGTATGTGGGAGATACTGATGCATGGTGTGAAGCCTTTTCAAGGAGTGAAGAACAATGA
TGTAATCGGTCGAATTGAAAATGGGGAAAGATTACCAATGCCTCCAAATTGTCCTCCTAC
CCTCTACAGCCTTATGACGAAATGCTGGGCCTATGACCCCAGCAGGCGGCCCAGGTTTAC
TGAACTTAAAGCTCAGCTCAGCACAATCCTGGAGGAAGAGAAGGCTCAGCAAGAAGAGCG
CATGAGGATGGAGTCCAGAAGACAGGCCACAGTGTCCTGGGACTCCGGAGGGTCTGATGA
AGCACCGCCCAAGCCCAGCAGACCGGGTTATCCCAGTCCGAGGTCCAGCGAAGGATTTTA
TCCCAGCCCACAGCACATGGTACAAACCAATCATTACCAGGACTCTACAGTATTGGACCT
GCGAGGGATTGGGCAAGTGTTGCCAACCCATCTGATGGAAGAGCGTCTAATCCGACAGCA
ACAGGAAATGGAAGAAGATCAGCGCTGGCTGGAAAAAGAGGAAAGATTTCTGAAACCTGA
TGTGAGACTCTCTCGAGGCAGTATTGACAGGGAGGATGGAAGTCTTCAGGGTCCGATTGG
AAACCAACATATATATCAGCCTGTGGGTAAACCAGATCCTGCAGCTCCACCAAAGAAACC
GCCTCGCCCTGGAGCTCCCGGTCATCTGGGAAGCCTTGCCAGCCTCAGCAGCCCTGCTGA
CAGCTACAACGAGGGTGTCAAGCTTCAGCCCCAGGAAATCAGCCCCCCTCCTACTGCCAA
CCTGGACCGGTCGAATGATAAGGTGTACGAGAATGTGACGGGCCTGGTGAAAGCTGTCAT
CGAGATGTCCAGTAAAATCCAGCCAGCCCCACCAGAGGAGTATGTCCCTATGGTGAAGGA
AGTCGGCTTGGCCCTGAGGACATTATTGGCCACTGTGGATGAGACCATTCCCCTCCTACC
AGCCAGCACCCACCGAGAGATTGAGATGGCACAGAAGCTATTGAACTCTGACCTGGGTGA
GCTCATCAACAAGATGAAACTGGCCCAGCAGTATGTCATGACCAGCCTCCAGCAAGAGTA
CAAAAAGCAAATGCTGACTGCTGCTCACGCCCTGGCTGTGGATGCCAAAAACTTACTCGA
TGTCATTGACCAAGCAAGACTGAAAATGCTTGGGCAGACGAGACCACACTGAGCCTCCCC
TAGGAGCACGTCTTGCTACCCTCTTTTGAAGATGTTCTCTAGCCTTCCACCAGCAGCGAG
GAATTAACCCTGTGTCCTCAGTCGCCAGCACTTACAGCTCCAACTTTTTTGAATGACCAT
CTGGTTGAAAAATCTTTCTCATATAAGTTTAACCACACTTTGATTTGGGTTCATTTTTTG
TTTTGTTTTTTTCAATCATGATATTCAGAAAAATCCAGGATCCAAAATGTGGCGTTTTTC
TAAGAATGAAAATTATATGTAAGCTTTTAAGCATCATGAAGAACAATTTATGTTCACATT
AAGATACGTTCTAAAGGGGGATGGCCAAGGGGTGACATCTTAATTCCTAAACTACCTTAG
CTGCATAGTGGAAGAGGAGAGCATGAAGCAAAGAATTCCAGGAAACCCAAGAGGCTGAGA
ATTCTTTTGTCTACCATAGAATTATTATCCAGACTGGAATTTTTGTTTGTTAGAACACCC
TTCAGTTGCAATATGCTAATCCCACTTTACAAAGAATATAAAAGCTATATTTTGAAGACT
TGAGTTATTTCAGAAAAAACTACAGCCCTTTTTGTCTTACCTGCCTTTTACTTTCGTGTG
GATATGTGAAGCATTGGGTCGGGAACTAGCTGTAGAACACAACTAAAAACTCATGTCTTT
TTTCACAGAATAATGTGCCAGTTTTTTGTAGCAATGATATTTCTCTTGGAAGCAGAAATG
CTTTGTACCAGAGCACCTCCAAACTGCATTGAGGAGAAGTTCCAGAACCATCCCCTTTTT
CCATTTTTATATAATTTATAAAGAAAGATTAAAGCCATGTTGACTATTTTACAGCCACTG
GAGTTAACTAACCCTTCCTTGTATCTGTCTTCCCAGGAGAGAATGAAGCAAAACAGGAAT
TTGGTTTTCTTTTGATGTCCAGTTACACCATCCATTCTGTTAATTTTGAAAAAATATACC
CTCCCTTTAGTTTGTTGGGGGATATAAATTATTCTCAGGAAGAATATAATGAACTGTACA
GTTACTTTGACCTATTAAAAAGGTGTTACCAGTAAAGTTCTTGTTGTAAAAAAAAAAAAA
AA
Protein sequence predicted based on nucleotide sequence
MAAAYLDPNLNHTPNSSTKTHLGTGMERSPGAMERVLKVFHYFESNSEPTTWASIIRHGD ATDVRGIIQKIVDSHKVKHVACYGFRLSHLRSEEVHWLHVDMGVSSVREKYELAHPPEEW KYELRIRYLPKGFLNQFTEDKPTLNFFYQQVKSDYMLEIADQVDQEIALKLGCLEIRRSY WEMRGNALEKKSNYEVLEKDVGLKRFFPKSLLDSVKAKTLRKLIQQTFRQFANLNREESI LKFFEILSPVYRFDKECFKCALGSSWIISVELAIGPEEGISYLTDKGCNPTHLADFTQVQ TIQYSNSEDKDRKGMLQLKIAGAPEPLTVTAPSLTIAENMADLIDGYCRLVNGTSQSFII RPQKEGERALPSIPKLANSEKQGMRTHAVSVSETDDYAEIIDEEDTYTMPSTRDYEIQRE RIELGRCIGEGQFGDVHQGIYMSPENPALAVAIKTCKNCTSDSVREKFLQEALTMRQFDH PHIVKLIGVITENPVWIIMELCTLGELRSFLQVRKYSLDLASLILYAYQLSTALAYLESK RFVHRDIAARNVLVSSNDCVKLGDFGLSRYMEDSTYYKASKGKLPIKWMAPESINFRRFT SASDVWMFGVCMWEILMHGVKPFQGVKNNDVIGRIENGERLPMPPNCPPTLYSLMTKCWA YDPSRRPRFTELKAQLSTILEEEKAQQEERMRMESRRQATVSWDSGGSDEAPPKPSRPGY PSPRSSEGFYPSPQHMVQTNHYQDSTVLDLRGIGQVLPTHLMEERLIRQQQEMEEDQRWL EKEERFLKPDVRLSRGSIDREDGSLQGPIGNQHIYQPVGKPDPAAPPKKPPRPGAPGHLG SLASLSSPADSYNEGVKLQPQEISPPPTANLDRSNDKVYENVTGLVKAVIEMSSKIQPAP PEEYVPMVKEVGLALRTLLATVDETIPLLPASTHREIEMAQKLLNSDLGELINKMKLAQQ YVMTSLQQEYKKQMLTAAHALAVDAKNLLDVIDQARLKMLGQTRPH*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: chloramphenicol
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
