DNASU Plasmid | LCK (H. sapiens )<br>In pLDNT7_nFLAG (Flag-tagged<br>In vitro expression vector)

Explanation of Terms

Gene:

Gene Symbol: LCK

Gene Name: lymphocyte-specific protein tyrosine kinase

Original Clone ID:

HsCD00299427

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pLDNT7_nFLAG

Format:

CLOSED

Source:

Center for Personalized Diagnostics

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Center for Personalized Diagnostics

HsCD00617935

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1530nts

Open reading frame: 52 to 1581

CGCCTGGACCATGTGAATGGGGCCAGAGGGCTCCCGGGCTGGGCAGGGACCATGGGCTGT
GGCTGCAGCTCACACCCGGAAGATGACTGGATGGAAAACATCGATGTGTGTGAGAACTGC
CATTATCCCATAGTCCGACTGGATGGGAAGGGCAGGCTGCTCATCCGAAATGGCTCTGAG
GTGCGGGACCCACTGGTTACCTACGAAGGCTCCAATCCGCCGGCTTCCCCACTGCAAGAC
AACCTGGTTATCGCTCTGCACAGCTATGAGCCCTCTCACGACGGAGATCTGGGCTTTGAG
AAGGGGGAACCACTCCGCATCCTGGAGCAGAGCGGCGAGTGGTGGAAGGCGCAGTCCCTG
ACCACGGGCCAGGAAGGCTTCATCCCCTTCAATTTTGTGGCCAAAGCGAACAGCCTGGAG
CCCGAACCCTGGTTCTTCAAGAACCTGAGCCGCAAGGACGCGGAGCGGCAGCTCCTGGCG
CCCGGGAACACTCACGGCTCCTTCCTCATCCGGGAGAGCGAGAGCACCGCCGGGTCCTTT
TCACTGTCGGTCCGGGACTTCGACCAAAACCAGGGAGAGGTGGTGAAACATTACAAGATC
CGTAATCTGGACAACGGTGGCTTCTACATCTCCCCTCGAATCACTTTTCCCGGCCTGCAT
GAACTGGTCCGCCATTACACCAATGCTTCAGATGGGCTGTGCACACGGTTGAGCCGCCCC
TGCCAGACCCAGAAGCCCCAGAAGCCGTGGTGGGAGGACGAGTGGGAGGTTCCCAGGGAG
ACGCTGAAGCTGGTGGAGCGGCTGGGGGCTGCACAGTTCGGGGAGGTGTGGATGGGGTAC
TACAACGGGCACACGAAGGTGGCGGTGAAGAGCCTGAAGCAGGGCAGCATGTCCCCGGAC
GCCTTCCTGGCCGAGGCCAACCTCATGAAGCAGCTGCAACACCAGCGGCTGGTTCGGCTC
TACGCTGTGGTCACCCAGGAGCCCATCTACATCATCACTGAATACATGGAGAATGGGAGT
CTAGTGGATTTTCTCAAGACCCCTTCAGGCATCAAGTTGACCATCAACAAACTCCTGGAC
ATGGCAGCCCAAATTGCAGAAGGCATGGCATTCATTGAAGAGCGGAATTATATTCATCGT
GACCTTCGGGCTGCCAACATTCTGGTGTCTGACACCCTGAGCTGCAAGATTGCAGACTTT
GGCCTAGCACGCCTCATTGAGGACAACGAGTACACAGCCAGGGAGGGGGCCAAGTTTCCC
ATTAAGTGGACAGCGCCAGAAGCCATTAACTACGGGACATTCACCATCAAGTCAGATGTG
TGGTCTTTTGGGATCCTGCTGACGGAAATTGTCACCCACGGCCGCATCCCTTACCCAGGG
ATGACCAACCCGGAGGTGATTCAGAACCTGGAGCGAGGCTACCGCATGGTGCGCCCTGAC
AACTGTCCAGAGGAGCTGTACCAACTCATGAGGCTGTGCTGGAAGGAGCGCCCAGAGGAC
CGGCCCACCTTTGACTACCTGCGCAGTGTGCTGGAGGACTTCTTCACGGCCACAGAGGGC
CAGTACCAGCCTCAGCCTTGAGAGGAGGCCTTGAGAGGCCCTGGGGTTCTCCCCCTTTCT
CTCCAGCCTGACTTGGGGAGATGGAGTTCTTGTGCCATAGTCACATGGCCTATGCACATA
TGGACTCTGCACATGAATCCCACCCACATGTGACACATATGCACCTTGTGTCTGTACACG
TGTCCTGTAGTTGCGTGGACTCTGCACATGTCTTGTGCATGTGTAGCCTGTGCATGTATG
TCTTGGACACTGTACAAGGTACCCCTTTCTGGCTCTCCCATTTCCTGAGACCACCAGAGA
GAGGGGAGAAGCCTGGGATTGACAGAAGCTTCTGCCCACCTACTTTTCTTTCCTCAGATC
ATCCAGAAGTTCCTGAAGGGCCAGGACTTTATCTAATACCTCTGTGTGCTCCTCCTTGGT
GCCTGGCCTGGCACACATCAGGAGTTCAATAAATGTCTGTTGATGACTGCCG

Protein sequence predicted based on nucleotide sequence

MGCGCSSHPEDDWMENIDVCENCHYPIVRLDGKGRLLIRNGSEVRDPLVTYEGSNPPASP
LQDNLVIALHSYEPSHDGDLGFEKGEPLRILEQSGEWWKAQSLTTGQEGFIPFNFVAKAN
SLEPEPWFFKNLSRKDAERQLLAPGNTHGSFLIRESESTAGSFSLSVRDFDQNQGEVVKH
YKIRNLDNGGFYISPRITFPGLHELVRHYTNASDGLCTRLSRPCQTQKPQKPWWEDEWEV
PRETLKLVERLGAAQFGEVWMGYYNGHTKVAVKSLKQGSMSPDAFLAEANLMKQLQHQRL
VRLYAVVTQEPIYIITEYMENGSLVDFLKTPSGIKLTINKLLDMAAQIAEGMAFIEERNY
IHRDLRAANILVSDTLSCKIADFGLARLIEDNEYTAREGAKFPIKWTAPEAINYGTFTIK
SDVWSFGILLTEIVTHGRIPYPGMTNPEVIQNLERGYRMVRPDNCPEELYQLMRLCWKER
PEDRPTFDYLRSVLEDFFTATEGQYQPQP*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin
Host Type: bacterial Marker: chloramphenicol

Bacterial Selection Condition: 100 ug/mL ampicillin, 34 ug/mL chloramphenicol

Growth Condition: Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.

Comments: Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pLDNT7_nFLAG

Synonyms:

pLDNT7-Flag

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning

Comments:

None

Size (bp):

4557

Parent Vector:

None

Properties:

Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker

Author Name:

Daniel Schiwek

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

GGGCGAATTAATTCCGGTTATTTTCCACCATATTGCCGTCTTTTGGCAATGTGAGGGCCCGGAAACCTGGCCCTGTCTTCTTGACGAGCATTCCTAGGGGTCTTTCCCCTCTCGCCAAAGGAATGCAAGGTCTGTTGAATGTCGTGAAGGAAGCAGTTCCTCTGGAAGCTTCTTGAAGACAAACAACGTCTGTAGCGACCCTTTGCAGGCAGCGGAACCCCCCACCTGGCGACAGGTGCCTCTGCGGCCAAAAGCCACGTGTATAAGATACACCTGCAAAGGCGGCACAACCCCAGTGCCACGTTGTGAGTTGGATAGTTGTGGAAAGAGTCAAATGGCTCACCTCAAGCGTATTCAACAAGGGGCTGAAGGATGCCCAGAAGGTACCCCATTGTATGGGATCTGATCTGGGGCCTCGGTGCACATGCTTTACATGTGTTTAGTCGAGGTTAAAAAACGTCTAGGCCCCCCGAACCACGGGGACGTGGTTTTCCTTTGAAAAACACGATGATAATATGGATCGGATCCGAATTCGAGCTCCGTCATCAACAAGTTTGTACAAAAAAGCAGGCTCCACCATGGCCGACTACAAGGACCACGACGGCGACTACAAGGACCACGACATCGACTACAAGGACGACGACGACAAGAGATCTATAACTTCGTATAGCATACATTATACGAAGTTATTGATCCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTTTGGACCCAGCTTTCTTGTACAAAGTGGTTGATGACAAGCTTGCGGCCGCACTCGAGCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCACTCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATGAGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTGGAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACAGTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTGGTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTGGATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAACTCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCGAAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCATCCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCCAATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTGAAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCATGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCCAAAATCGGATCTGGTTCCGCGTTGAAGATCTGACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTTTAAACACTAGTCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGAAATTGTAAACGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATTGTAATACGACTCACTATA

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ColE1 ori

ColE1 origin of replication

1842

2524

promoter

T7 promoter

4462

4479

recombination site

LoxP

LoxP site for recombinational cloning

657

690

selectable marker

AmpR

ampicillin resistance gene

2622

3281

ssDNA origin

f1 ori

f1 origin for ssDNA production

3928

4368

tag

Flag

3xFlag epitope tag ORF and LoxP region

585

650

trxn termination sequence

T7 term

T7 terminator sequence

1614

1660

Gene

Gene Symbol :

LCK

Symbol Nomenclature :

LCK

SYNONYM :

LSK; YT16; p56lck; pp58lck

Designation :

Full Nomenclature :

lymphocyte-specific protein tyrosine kinase

GENEID :

3932

Locus Tag :

RP4-675E8.4

HGNC :

6524

MIM :

153390

Vega :

OTTHUMG00000007463

Target GenBank:

NM_005356

Pathways

NCI :

Alpha-synuclein signaling

NCI :

amb2 Integrin signaling

NCI :

Atypical NF-kappaB pathway

NCI :

Class I PI3K signaling events

NCI :

CXCR4-mediated signaling events

NCI :

EPHA forward signaling

NCI :

Ephrin B reverse signaling

NCI :

Glypican 1 network

NCI :

IL12-mediated signaling events

NCI :

IL2 signaling events mediated by PI3K

NCI :

IL2 signaling events mediated by STAT5

NCI :

IL2-mediated signaling events

NCI :

PDGFR-beta signaling pathway

NCI :

Regulation of p38-alpha and p38-beta

NCI :

SHP2 signaling

NCI :

Signaling events mediated by PTP1B

NCI :

TCR signaling in naïve CD4+ T cells

NCI :

TCR signaling in naïve CD8+ T cells

NCI :

Thromboxane A2 receptor signaling

Panther :

Parkinson disease

Panther :

T cell activation

Reactome :

Adaptive Immune System

Reactome :

CD28 co-stimulation

Reactome :

CD28 dependent PI3K/Akt signaling

Reactome :

CD28 dependent Vav1 pathway

Reactome :

Cell surface interactions at the vascular wall

Reactome :

Constitutive PI3K/AKT Signaling in Cancer

Reactome :

Costimulation by the CD28 family

Reactome :

CTLA4 inhibitory signaling

Reactome :

Cytokine Signaling in Immune system

Reactome :

DAP12 interactions

Reactome :

DAP12 signaling

Reactome :

Disease

Reactome :

Downstream signal transduction

Reactome :

Downstream signaling events of B Cell Receptor (BCR)

Reactome :

Downstream signaling of activated FGFR

Reactome :

Downstream TCR signaling

Reactome :

Fc epsilon receptor (FCERI) signaling

Reactome :

GAB1 signalosome

Reactome :

Generation of second messenger molecules

Reactome :

GPVI-mediated activation cascade

Reactome :

Hemostasis

Reactome :

HIV Infection

Reactome :

Host Interactions of HIV factors

Reactome :

Immune System

Reactome :

Innate Immune System

Reactome :

Interleukin-2 signaling

Reactome :

Nef and signal transduction

Reactome :

Nef Mediated CD4 Down-regulation

Reactome :

Nef-mediates down modulation of cell surface receptors by recruiting them to clathrin adapters

Reactome :

NGF signalling via TRKA from the plasma membrane

Reactome :

PD-1 signaling

Reactome :

PECAM1 interactions

Reactome :

Phosphorylation of CD3 and TCR zeta chains

Reactome :

PI-3K cascade

Reactome :

PI3K events in ERBB2 signaling

Reactome :

PI3K events in ERBB4 signaling

Reactome :

PI3K/AKT activation

Reactome :

PI3K/AKT Signaling in Cancer

Reactome :

PIP3 activates AKT signaling

Reactome :

Platelet activation, signaling and aggregation

Reactome :

Regulation of KIT signaling

Reactome :

Role of LAT2/NTAL/LAB on calcium mobilization

Reactome :

Signal Transduction

Reactome :

Signaling by EGFR

Reactome :

Signaling by EGFR in Cancer

Reactome :

Signaling by ERBB2

Reactome :

Signaling by ERBB4

Reactome :

Signaling by FGFR

Reactome :

Signaling by FGFR in disease

Reactome :

Signaling by Interleukins

Reactome :

Signaling by PDGF

Reactome :

Signaling by SCF-KIT

Reactome :

Signaling by the B Cell Receptor (BCR)

Reactome :

Signalling by NGF

Reactome :

TCR signaling

Reactome :

The role of Nef in HIV-1 replication and disease pathogenesis

Reactome :

Translocation of ZAP-70 to Immunological synapse

WikiPathway :

IL-2 Signaling pathway

WikiPathway :

Inflammatory Response Pathway

WikiPathway :

Notch Signaling Pathway

Protein

SMART domain :

TyrKc : Tyrosine kinase, catalytic domain

SMART domain :

SH3 : Src homology 3 domains

SMART domain :

S_TKc : Serine/Threonine protein kinases, catalytic domain

SMART domain :

SH2 : Src homology 2 domains

UniProt :

P06239

HPRD :

01080

Clone

Cloning Information :

654695

HIP Master Clone ID :

14591

Original Clone ID :

FLH231676.01L

Original Clone ID :

HsCD00299427

Organism

Species Specific ID:

3932

Genome

Chromosome :

Map Location :

1p34.3

Ensembl :

ENSG00000182866

Reagents

Labome :

Lck-antibody

LCK (H. sapiens )In pLDNT7_nFLAG (Flag-taggedIn vitro expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Pathways

Protein

Clone

Organism

Genome

Reagents

LCK (H. sapiens )
In pLDNT7_nFLAG (Flag-tagged
In vitro expression vector)